Objective To investigate the effect of tunor necrosis factor alpha (TNF-α) antagonist treatment and traditional disease-modifying antirheumatic drugs treatment on the quality of life of male patients with ankylosing spondylitis (AS).Methods In 42 patients with AS open-label study,patients were grouped after consent,and divided into the TNF-α antagonist treatment group and the non-TNF-α antagonist treatment group.All patients were treated for 4 months.The clinical and laboratory indexes and quality of life changes were analyzed before and after the treatment.T test,Pearson's correlation analysis were used for statistical analysis with software version 13.0.Results There were no significant differences in age,disease course,disease activity and the quality of life between the two groups before treatment.Disease activity and the quality of life of the two groups after treatment were improved compared with that of before treatment.After treatment,the Bath ankylosing spondylitis disease activity index (BASDAI) score [(1.9±1.6) scores,(3.0±1.3) scores,t=-2.429,P=0.020],erythrocyte sedimentation rate (ESR) [(9±6) mm/1 h,(18±17) mm/1 h,t=-2.286,P=0.031] and C reactive protein(CRP) levels [(18±21) mg/L,(62±85) mg/L,t=-2.258,P=0.035] of the TNF-α antagonists treatment group decreased significantly than those non-TNF-α antagonist treatment group,while the hemoglobin (Hb) levels [(143±15) g/L,(138±18) g/L,t=2.545,P=0.015] were significantly increased in TNF-α antagonist treatment group.The improvement extent of quality of life was more evident in TNF-α antagonists treatment group,such as,the average score [(72± 15) scores,(55±19) scores,t =3.254,P=0.002].The average degree of improvement in quality of life and BASDAI score,Bath ankylosing spondylitis functional index (BASFI) score were negatively related to the improvement in the TNF-α treatment group (r=-0.497,P=0.018; r=-0.558,P=0.007).Conclusion TNF-α antagonist treatment can not only improve the AS disease activity,but can also improve the quality of sexual life of male patients,which may direct affect on male reproductive system.

Objective To discuss the repair methods for the cartilage defects of the knee joint. Methods The cylindrical osteochondral grafts were harvested from the non-weight-bearing area of the knee joint and then transplanted to the cartilage defects in 6 patients with cartilage defects of the knee joint. Results The clinical symptoms of all the patients followed up for 2-24 months disappeared and the joint mobility recovered to normal. A follow-up MRI showed consistent cartilage coverage and the excellent position of the cylindrical osteochondral grafts. Conclusions The osteochondral autograft is a practical surgical method because it is characterized by less trauma, simple performance and good maintenance of the curvature of the articular surface.

The paper states the superiority of optimizing the teaching effect of clinical medicine by making use of information technology,analyzes the problems of information technology in optimizing the classroom teaching of clinical medicine,discusses the strategy of teaching with information technology in the aspect of classroom teaching structure,and thus reaches the effect of improving teaching efficiency and quality.

The myocardial nutrition after myocardial revascu-larization was studied in 14 rabbits with the aid of Nd-YAG laser, before Ligation of the anterior descending branch of the left coronary artery (only ligated LAD in 4 rabbit), the rabbits were seperatively Killed 3 or 5 weekafter operating. Myocardial samples were abtained in the parti of 0 - 2,2 - 4,4 - 6mm from laser hole. Sample numbers were 30 in myocardial revascularization group (MRG); 20 in myocardial Infarction group(MIG); 20 in normal group (NG). Amount of ATP and CP in myocar-dia were determined with luciferase. Resalts.ATP and CP near laser holes were normal 3 week late. This far from laser holes was lower than that of normal myocardia 5 week late. Recover of CP amount was slower than that of ATP amount. Inclusion, myocardial revascularization can play role in myocardial nutrition.

Cytoskeletal Proteins ; biosynthesis ; genetics ; physiology ; Humans ; Neoplasm Metastasis ; Neoplasms ; pathology ; Signal Transduction

Endoscopy ; methods ; Humans ; Neck ; surgery

Animals ; Bone Neoplasms ; pathology ; Cell Adhesion ; Cell Culture Techniques ; methods ; Coculture Techniques ; Drug Screening Assays, Antitumor ; methods ; Humans ; Neoplasm Invasiveness ; Tumor Cells, Cultured

Objective To establish a high-performance liquid chromatography method for determination of mycophenolic acid in human plasma.Methods The method was performed with C18 column(3.9mm?300mm).The mobile phase consisted of 40mmoL/L TBA solution-acetonitrile adjusted to pH 4 with orthophosphoric acid(55∶45,V∶V),with a flow rate 0.8mL/min.The UV detection wavelength was 254nm.Plasma samples were extracted with methanol.Results The linear concentration of the calibration curve ranged from 0.8 to 51.45mg/L(r=0.9998).The relative recoveries were between 95.5% and 109.2%,and the extraction recoveries were more than 90%.Relative standard deviation(RSD) of intraday and interday assays were both less than 10%.Conclusion The method is proved to be reliable and simple for monitoring mycophenolic acid in human plasma and bioequivalence research.

Objective: To discuss the rational drug application of Kawasaki disease in children with upper respiratory tract infection in order to provide reference for the therapy of Kawasaki disease suspected of bacterial meningitis.Methods: The medical record of a 2-year-old boy with Kawasaki disease was retrospectively analyzed, and the anti-infection treatment was analyzed and evaluated.Meanwhile, the mechanism of elevated leukocyte count in cerebrospinal fluid (CSF)of children with incomplete Kawasaki disease was discussed.The pharmaceutical care and treatment results in the diagnosis and treatment process were analyzed as well.Results: The anti-infective therapy in early stage was reasonable as the early symptoms of respiratory tract infection in the child were obvious, however, the choice of aztreonam was defective.On the premise of the clinical diagnosis of Kawasaki disease and excluding bacterial meningitis, the medication was timely adjusted.Finally, the symptoms of the child were improved.Conclusion: The application of anti-infection drugs should be reasonable in clinics, and the abnormality in cerebrospinal fluid should be paid more attention in children with Kawasaki disease.It is necessary to strengthen clinical observation and pharmaceutical care in order to guarantee the safety of medication for patients.