[Objective]To study the possibility of differentiation of rabbit marrow mesenchymal stem cells(MSCs)into endothelial cells in vitro.[Method]BMSCs of rabbits were obtained by using gradient centrifuge method.The adhesive cells were preserved to passage culture to get pure MSCs.MSCs in the second generation were used.After induction with vascular endothelial growth factor(VEGF),microscopic exarm,cytoimmunofluorescent staining confirmd the trial group cells becomes to endothelial cells.W-P corpuscles which was the symbol of endothelial cells were observed by transmission electron microscope.[Result]The differentiated cells demonstrated the characters of endothelial cells under phase contrast microscopy.The cytoimmunofluore-scent staining showed that the trial group cells were induced to endothelial cells.W-P corpuscles could be seen under transmission electron microscope.[Conclusion]The adult rabbit BMSCs can present features of endothelial cells in certain induction medium,they are ideal donor cells of tissue engineering vascularization.

In order to overcome the problems existing in traditional teaching of elemental chemistry experiment,a series of reformations including teaching method have been carried out,and good results have been achieved.

A series of reform on the teaching of medical chemistry has been carried out to improve the quality of instruction,which includes the goal of instruction,the content of course and the teaching method.Good results have been achieved.

Urinary incontinence in the elderly is a multifactorial geriatric syndrome with a high prevalence and low rates of diagnosis and treatment.Evaluation of urinary incontinence is the basis for treatment of elderly patients with urinary incontinence and includes risk factors,medical history,physical examinations and supplementary examinations.Treatment of urinary incontinence in the elderly should follow the principles of noninvasive treatment before invasive treatment and behavioral and physical therapy before drug therapy.Behavioral therapy mainly includes lifestyle intervention,bladder training,and pelvic floor muscle training.Physical therapy includes electrical and magnetic stimulation.When the effect of behavioral therapy combined with physical therapy is poor,drug treatment can be added.

The Chinese herbal compound formula preparation,which is guided by traditional Chinese medicine (TCM) theories,and based on long-term clinical application,has its unique value.The multi-compound and multi-target characteristics are also features of Chinese materia medica (CMM).The finding and exploration of innovation CMM in modern times should be based on TCM theory and clinical application,as well as the combination of efficacy in the concept of holism and biomedical technology.Key issue in the research,development and evaluation of innovation Chinese medicine is to use to the methodology to illustrate the scientific meaning in according to the clinical application characteristics of Chinese herbal compound formula preparation.During the research and development process,considerations on CMM characteristics should be taken from research evidence,to pharmacodynamics and toxicology studies.Pharmacodynamics researches should reflect the properties of TCM syndrome.Aspects,such as research and application of TCM syndrome model,correspondence between disease and syndrome model,research method of correspondence between syndrome and prescription,identification of biological markers corresponding to CMM pharmacologic effect,are important.So we should catch up the historical chance for TCM development,speedily develop the advantage areas,and promote the modernization and innovation of TCM.

Objective To investigate the expressions and clinical significances of Runt-domain-related 3 (Runx3) and chromodomain helicase DNA-binding protein 5 (CHD5) in colorectal cancer.Methods Ninety-six colorectal cancer tissue samples and matched adjacent normal tissues and 72 colorectal adenoma tissues were collected.Real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting were used to detect the mRNA and protein expression of Runx3 and CHD5.The associations of Runx3 and CHD5 expression with clinical pathological characteristics,diagnostic value and prognosis relationship of patients were further analyzed.Results Runx3 and CHD5 relative expressions of mRNA and protein were 0.35 ± 0.00,0.28 ± 0.02 and 0.26 ± 0.02,0.31 ± 0.01,which were significantly lower than those in the matched adjacent tissues 0.95 ± 0.02,0.92 ± 0.02 and 0.89 ± 0.03,0.93 ± 0.02 (t =2.36,P ＜ 0.05;t =1.25,P ＜ 0.05;t =1.37,P ＜ 0.05;t =1.13,P ＜ 0.05) and colorectal adenoma tissues 0.89 ± 0.02,0.90 ± 0.02 and 0.85±0.02,0.87±0.04 (t=2.27,P＜0.05;t=2.16,P＜0.05;t=1.25,P＜0.05;t=2.65,P＜0.05).Runx3 and CHD5 expressions differed significantly between tumors with different TNM stages (x2 =4.65,P =0.031;x2 =7.89,P =0.005),depths of tumor invasion (x2 =4.17,P =0.041;x2 =4.86,P =0.028),lymph node statuses (x2 =4.20,P =0.040;x2 =7.02,P =0.008),or histological differentiation (x2 =7.31 P =0.036;x2 =9.54,P =0.023).Linear correlation analysis showed that the expressions of the two genes were positively correlated (r =0.572,P =0.001).Receiver operating characteristic (ROC) curve showed that Runx3 and CHD5 had diagnostic value (AUC were 0.712,0.745;sensitivity and specificity were 45.9％,52.5％ and 83.6％,81.4％ respectively).Runx3 and CHD5 both low expression group compared with the other patient groups in overall survival time (x2 =8.156,P ＜ O.05) and progression-free survival (x2 =6.325,P ＜ 0.05) had statistically significant differences.Conclusion Runx3 and CHD5 are low expressed in colorectal cancer and may prove useful as biomarkers for diagnosis target and prognostic indication in patients with colorectal cancer.

Computerized intermediate-frequency therapy apparatus is a kind of widely-used clinical equipment. This paper is to indicate the principle and design method of the central control system based on USB for computerized intermediate-frequency therapy apparatus. With this system, the labor intensity, work efficiency and therapeutic efficacy can all be improved.

OBJECTIVE: To studies on the stability of glucosamine-?-aminoacids-nickel(Ⅱ) ternary complexes(GANTC). METHODS: The formation constant of GANTC were determined at (25?0.1) ℃,I=0.1 mol?L-1 KNO3 by pH method. RESULTS: The formation constant of GANTC were Gly 13.73(7.33),Pro 13.97(7.12),Ser 13.15(7.07),Val 13.46(6.84),iLe 13.55(6.88),Phe 13.13(6.93) and Met 13.17(7.18). CONCLUSIONS: The two biologically active ligand,?-aminoacid and glucosamine accommodate each other when coordinated to nickel(Ⅱ) ion. The formation constant of the ternary complexes linearly increase with the increase of protonation constant of aminoacid. Linear free energy relationship does exist in the presence of ternary system.

Objective To know a kind of method to prevent contamination of water system in department of stomatology. Methods Divided 120 patients with dental diseases into the control group and the observation group randomly, there were 60 cases in each group. Replace the holding tank of wa-ter and distilled water per 24 hours was used in the control group, while the method of replace the hold-ing tank of water and distilled water for each person was used in the observation group, and then com-pared the contamination condition of water system between the two groups. Results The percent of pass of water system in the observaiton group was 100%, which significant higher than the 68.33% in the control group. Conclusions Replace the holding tank of water and distilled water for each person is a kind of method to avoid contamination of water system in department of stomatology.

Objective:To compare the content of polydatin and emodin between polygonum cuspidatum pieces and dispensing gran-ules to provide theoretical basis for the improvement of preparation process of the granules. Methods:An HPLC method with linear gra-dient elution was used to determine the content of polydatin and emodin in the pieces and dispensing granules, and the difference in the two contents was compared. Results: The content of polydatin and emodin in polygonum cuspidatum pieces met the requirement de-scribed in China pharmacopoeia, which was lower than that in the dispensing granules, and the contents of the two components in the commercial dispensing granules were not equivalent to the labeled amount, which was 1/15 and 1/27 of that in the pieces. Conclu-sion:The transport rate of the traditional preparation process of dispensing granules is with poor efficiency, which should be improved.