Objective:To investigate the molecular mechanisms of proliferation and apoptosis by penta-brominated Diphenyl Ethers through regulation of mRNA and protein expression of Bcl-2 and Bax in breast cancer MCF-7 cells.Methods:Human breast cancer MCF-7 cells were grown in DMEM medium,MTT assay was employed to investigate cell proliferation.RT-PCR and immunohistochemistry was performed to determine mRNA and protein expression of Bcl-2 and Bax,Results:Within the range of 1?(10~(-8)～10~(-4))mol/L,5-BDEs stimulated proliferation in MCF-7 cells with a dose-dependent and time-dependent fashion.RT-PCR and immunohistochemistry analyses revealed that treatment with 1?(10~(-8)～10~(-4))mol/L for 72h resulted in increased mRNA and protein expression of Bcl-2 and inhibition of Bax.Conclusion:5-BDE possessed estrogenic activity and could markedly stimulate proliferation in estrogen responsive breast cancer MCF-7 cells.Up-regulation of Bcl-2 mRNA and protein expression was suggested to be involved in this biological effect.

Objective To investigate the effect and function analysis by sulfentanyl in patients with tongue cancer postoperative analgesia.Methods A total of 74 patients with radical resection of tongue cancer in department of anesthesiology with general anesthesia from our hospital were collected, patients or their families signed consent, according to postoperative self-controlled intravenous analgesia drugs divided into experimental group and control group with 37 cases in each group.Patients in control group were treated by fentanyl self-controlled intravenous analgesia;patients in experimental group were treated by sulfentanyl self-controlled intravenous analgesia, determination of the analgesic effect, sedative effect and the changed of vital signs postoperative 2, 4, 8, 12, 24, 48 h,at the same time recorded complications.Results After operation, at different time points analgesic effect and sedative effect were changed in the two groups, the VAS scored of the experimental group was lower than the control group postoperative 2, 4, 8, 12, 24, 48 h time points, the Ramsay Sedation scored of the experimental group was lower than the control group postoperative 2 h, 4 h, 8 h, 12 h time points ( P <0.05 ); the changed of vital signs and adverse reactions between the two groups had no difference within 48 hours ( P >0.05 ) . Conclusion Sulfentanyl in the treatment of patients with radical resection of tongue cancer the analgesic effect and sedative effect are better than fentanyl, has no effect on the vital signs, and the role is security and exact.

OBJECTIVETo investigate the effects of closed reduction and ulna intramedullary nail fixation for the treatment of fresh Monteggia fractures of Bado type I and II in children.

METHODSTwenty-three children patients with Monteggia fracture during July 2010 to September 2013 were treated by closed reduction and ulna intramedullary nail fixaion including 18 boys and 5 girls with an average age of 9.3 years old ranging from 6 to 13 years old. Among them,15 cases were Bado type I and 8 cases were Bado type II. There were 9 cases with radial nerve injury. The operation time,the recovery of nerve injury, the fracture healing and the function of elbow were observed and recorded.

RESULTSAll patients were followed up for 6 to 24 months (12 months on average). All patients were obtained bone healing. According to Anderson standard, at the final follow-up, 20 cases got excellent result, 2 cases got good result, and one case got fair result.

CONCLUSIONTreatment of the fresh Monteggia fractures in children by closed reduction and ulna intramedullary nail fixation has advantages of simple operation, less trauma and good results.

Adolescent ; Child ; Female ; Fracture Fixation, Intramedullary ; methods ; Fracture Healing ; Humans ; Male ; Monteggia's Fracture ; surgery ; Ulna ; surgery

Allergens ; analysis ; Humans ; Hypersensitivity ; diagnosis ; immunology ; Practice Guidelines as Topic ; Skin Tests

OBJECTIVETo evaluate the effect of repeated sintering on the color and translucency of dental lithium disilicate-based glass ceramic.

METHODSThirty disc specimens (10 mm in diameter, and 1.00 mm ± 0.01 mm in thickness) were fabricated using an IPS e.max Press HT A2 ingot and then randomly divided into six groups (SO, S1, S2, S3, S4, and S5) (n = 5). Each group was sintered 0, 1, 2, 3, 4, and 5 times individually according to the manufacturer's recommendation. After polishing, ultrasonic cleaning, and drying, the color parameters (L*, a*, and b* values) and transmittance (τ) of all the specimens were measured using a ShadeEye NCC dental colorimeter and a TM-2 spectrophotometer, respectively. Subsequently, C*ab and ΔE were calculated. The results were statistically analyzed using SPSS 17.0 software for ANOVA and LSD.

RESULTSAfter repeated sintering, the L* value significantly decreased (P < 0.05), and the a*, b*, and C*ab values initially increased and then decreased (P < 0.05). The color parameters (L*, a*, and b*) and the C*ab values of group S3 were higher than those of the other groups. The τ values initially increased and then decreased (P < 0.05), and these values were optimal when sintered twice. In terms of transmittance (τ), statistical differences existed between the following groups: S0 and S2, S0 and S3,.S2 and S5, and S3 and S5 (P < 0.05). Compared with group S0, the color differences ΔE were 0.89 minimally and 2.01 maximally after different sintering times, which can be clinically acceptable.

CONCLUSIONRepeated sintering can affect the color and translucency of the IPS e.max Press lithium disilicate-based glass ceramic, but the color difference can be clinically acceptable.

Ceramics ; Color ; Colorimetry ; Dental Materials ; Dental Porcelain ; Random Allocation ; Spectrophotometry

Objective Analyse the causes,diagnosis and treatment for delayed hemorrhage after distal radical gastrectomy.Methods Retrospective study on 45 patients combined with intro-abdominal hemorrhage after distal radical gastrectomy from January 2008 to June 2013.Results Thirteen patients combined with delayed hemorrhage in these 45 patients,all of 13 patients had intro-abdominal hemorrhage in 1week to 4 weeks after operation.And 5 of the 13 patiens were intermittent intro-abdominal hemorrhage 1 week after operation,these patients were demonstrated the blood come from gastroduodenal artery pseudoaneurysm fracture by CT and DSA examine,and they were cured by interventional embolization.Other 8 patients were marginal ulcer hemorrhage diagnosed by gastroscope,and they stoped bleeding with the help of gastroscope.Conclusions The causes of delayed hemorrhage after distal radical gastrectomy were complicated,and CT,DSA and endoscope can use for diagnosis.What was more,interventional embolization and endoscope were helpful for curing the intro-abdominal hemorrhage,avoiding re-operation.

The incidence rate of erectile dysfunction (ED) is reportedly as high as 30-90% after radical prostatectomy for prostate cancer, which seriously affects the patients' quality of life. Penile rehabilitation is defined as the use of any drug or device at or after radical prostatectomy to maximize erectile function recovery. A variety of treatment options are available for post-prostatectomy ED patients, including oral phosphodiesterase 5 inhibitors (PDE5I) , intracorporal injections, vacuum erection device, and penile prosthesis. This article presents an overview of the currently used methods for the drug treatment and penile rehabilitation of the ED patients after radical prostatectomy. It seems proper to recommend daily use of a vacuum erection device plus oral PDE5I in the early postoperative period. For those who fail to respond to this therapy, intraurethral alprostadil, intracorporal injections, or a penile prosthesis could be considered.
Alprostadil ; administration & dosage ; Combined Modality Therapy ; Erectile Dysfunction ; etiology ; therapy ; Humans ; Male ; Penile Erection ; Penile Prosthesis ; Phosphodiesterase 5 Inhibitors ; administration & dosage ; Prostatectomy ; adverse effects ; Quality of Life ; Recovery of Function ; Vacuum

Adult ; Aged ; Body Mass Index ; Case-Control Studies ; Humans ; Male ; Middle Aged ; Neck ; anatomy & histology ; Sleep Apnea, Obstructive ; pathology ; physiopathology ; Young Adult

OBJECTIVE:To survey and evaluate the present situation and trend of the anticancer drugs used in 6 cities of the Yangtse valley METHODS:The anticancer drugs,used in 138 hospitals of 6 cities in the Yangtse basin during the period 1999～2001,were analysed in respect to the cost,sales volume of pharmaceutical enterprises and situation of clinical application RESULTS:The costs of anticancer drugs consumption were 19 460,24 421 and 32 227 million yuan in 1999,2000 and 2001,respectively with an average annual cost of 10 238 yuan per sickbed Some new anticancer drugs derived from nature plants and monoantibodies were going to the clinical practice And gene therapy has been introduced to the treatment of cancer CONCLUSION:The anticancer drug is an important kind of drugs used in hospitals The cost per sickbed was much high and was increasing year by year Furthermore,the anticancer drugs derived from nature plants,monoantibody and the gene therapy will be the predominant measures in the future

OBJECTIVE:To analyze the present situation and trend of the application of antituberculotics in hospitals of 6 cities METHODS:The antituberculotics used in 138 hospitals of 6 cities during the 1999～2001 period were analysed in respect to the cost,sales volume of pharmaceutical enterprises and situation of clinical application RESULTS:The costs of consumption of antituberculotics were 6 227 520,7 204 738 and 7 537 601 yuan in 1999,2000 and 2001 respectively with a rising trend year by year The average annual cost of antituberculotics was 282 yuan per sickbed CONCLUSION:The antituberculotics are fewer in kind and inexpensive in price but they occupy an important place in a hospital and their consumption is unceasingly increasing