1.Discussion on the Ultrasonic Extraction in the Determination of Chanfukang Granules
Xiaomeng ZHAO ; Chun DONG ; Jiejiao YANG
China Pharmacy 2001;0(09):-
OBJECTIVE: To investigate the effect of ultrasonic extraction in the determination of Chanfukang gran- ules. METHODS: Different types of ultrasonic extractor were used to determine the same sample repeatedly .RESULTS & CONCLUSION: The extraction effects were different in using ultrasonic extractors with different power levels and ultrasonic frequency.
2.Immunomodulators to be applied with good reason.
Xiao-dong ZHAO ; Xi-qiang YANG
Chinese Journal of Pediatrics 2006;44(6):401-402
4.Influences of Dichloromethane Extraction from Crude and Processed Cornus Officinalis on Bone Marrow Cells of Aging Mice Induced by D-galactose
Qingchun ZHAO ; Ju DONG ; Liu YANG
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(03):-
Objective To study the influences of dichloromethane extraction from crude and processed Cornus Officinalis on bone marrow cells of aging mice induced by D-galactose. Methods DNA damage of bone marrow cells of aging mice induced by D-galactose was detected by the comet assay, the expression of p53 mRNA was detected by RT-PCR. Results The tail formation rate of model group was significantly higher than the rate of control group (P
5.Value of Heparin Blocking Attachment of Respiratory Syncytial Virus to Human Airway Epithelial Cells
xiao-dong, ZHAO ; xi-qiang, YANG
Journal of Applied Clinical Pediatrics 2006;0(22):-
Objective To investigate the mechanisms of attachment of respiratory syncytial virus(RSV) to human airway epithelial cells.Methods Attachment of RSV to CFBE was quantitatively assessed by flow cytometry.Various polyclonal and monoclonal antibodies to RSV and heparin were pre-incubated with RSV.The blocking effects of these antibodies and heparin on attachment were evaluated.Results CFBE cells reduced capacity being bound by RSV.All the antibodies used were failed to block attachment of RSV on CFBE,whereas heparin blocked RSV attachment in a dose-response manner and the blokade by heparin was almost complete at the concentration of 0.4 mg/L.Conclusions Flow cytometry provides direct evaluation of attachment without growth of virus.Heparin-like molecules on cell surface of CFBE appears to be involved in attachment between RSV and human epithelial cells.
6.RNAi silencing MTA1 gene inhibits invasion and migration of esophageal carcinoma 9706 cells
Songhua YANG ; Guoqiang ZHAO ; Hong ZHENG ; Jimin ZHAO ; Ziming DONG
Journal of Third Military Medical University 1988;0(06):-
Objective To observe the effect of RNAi that silences MTA1 gene on invasion and migration of esophageal carcinoma 9706 cells. Methods The siRNA expression vector that silences MTA1 gene was transfected into EC9706 cells by liposome. MTA1 mRNA and protein expressions were detected through quantitative RT-PCR and Western blot, respectively. The invasion and migration of EC9706 cells were evaluated by scrape wound healing assay and cell invasion assay in vitro. Results MTA1 gene expression significantly decreased. The scrape wound of EC9706 cells healed more slowly and the cell population that cut through Matrigel were less in the EC9706 cells transfected with siRNA expression vector than non-transfected EC9706 cells and the EC9706 cells transfected with blank vector (P
7.Effect of Meibomian gland massage before phacoemulsification on ocular surface after surgery in patients with Meibomian gland dysfunction
Bang-Tao, YAO ; Xiao-Gui, ZHAO ; Zhao-Yang, DONG
International Eye Science 2016;16(6):1171-1173
?AIM:To study the changes of ocular surface in cataract patients with Meibomian gland dysfunction ( MGD) who treated with Meibomian gland massage before surgery.?METHODS: Totally 90 patients ( 93 eyes ) with cataract and MGD were randomly divided into two groups. Patients in experimental group were treated with hot compress and Meibomian gland massage every day before surgery, while the patients in the control group were not taken. Two groups of patients received phacoemulsification combined with intraocular lens implantation. Lid margin abnormalities, secretion characteristics, Schimer I test ( SⅠt) and tear film break-up time ( BUT ) were recorded and compared between two groups using slit lamp microscope inspection preoperatively and postoperatively 1wk.?RESULTS:In the control group, the postoperative score of eyelid margin and Meibomian gland secretion was increased significantly than preoperative, while the value of BUT and SⅠt was down significantly. Compared with control group, the postoperative score of eyelid margin shape and Meibomian gland secretion in the experimental group was decreased significantly, while the value of BUT and SIT improved significantly (P<0. 05).?CONCLUSION: Phacoemulsification can aggravate the Meibomian gland dysfunction and have some extent of effects on the ocular surface. Meibomian gland massage before surgery can significantly improve the function of Meibomian gland and the state of ocular surface in patients with MDG.
8.Construction of traditional Chinese medicine resources information spatial database.
Yu-yang ZHAO ; Cheng-zhong SUN ; Ze-dong YANG
China Journal of Chinese Materia Medica 2015;40(6):1219-1222
The informatization of traditional Chinese medicine resources is the basis of modern medicine. With a spatial attribute traditional Chinese medicine resources could be carried out for in-depth spatial analysis, data mining and traditional Chinese medicine resources regional industrial layout. In this paper, we took the data of Glycyrrhiza uralensis in the third national Chinese medicine resources survey as the experimental data, described the principles and structure of traditional Chinese medicine resources spatial information database. We also described the establishment of analysis model with the help of this spatial database.
Data Mining
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Databases, Factual
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Medicine, Chinese Traditional
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Plants, Medicinal
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chemistry
9.Pareto Chart Analysis of Unreasonable Factors of Prescription in Outpatient and Emergency Departments of Our Hospital during 2013-2015
Zhiqiang DONG ; Jianfeng ZHAO ; Yanmei LUO ; Guolong ZHANG ; Yang YANG
China Pharmacy 2017;28(17):2340-2342
OBJECTIVE:To investigate the current situation of unreasonable prescriptions in outpatient and emergency depart-ments of our hospital,and to provide reference for rational drug use in the clinic. METHODS:The evaluation of outpatient and emergency prescriptions in our hospital during 2013-2015 were summarized and analyzed. Irrational prescriptions were divided into 3 categories,involving 19 items. The main,secondary and general factors of irrational prescriptions were analyzed by Pareto chart. RESULTS:Among 19 types of irrational prescriptions,main factors included unsuitable drug selection,inappropriate usage and dosage,inappropriate drug combination,prescribing not in accordance with the antibiotics management regulations,incomplete di-agnosis. No indications of medication,drug dosage beyond the regulations ofthree urgent seven slowwere treated as the second-ary factors. Other 12 items were general factors. CONCLUSIONS:According to the main and secondary irrational drug use types, clinical pharmacists should carry out effective intervention and pharmaceutical care to improve the level of rational drug use and en-sure the safety of clinical drug use.