12E7 Antigen ; Adult ; Antigens, CD ; metabolism ; Calbindin 2 ; Cell Adhesion Molecules ; metabolism ; Female ; Follow-Up Studies ; Humans ; Hysterectomy ; methods ; Inhibins ; metabolism ; Middle Aged ; Ovarian Neoplasms ; metabolism ; pathology ; surgery ; Ovariectomy ; methods ; Retrospective Studies ; S100 Calcium Binding Protein G ; metabolism ; Sex Cord-Gonadal Stromal Tumors ; metabolism ; pathology ; surgery ; Young Adult

Objective To investgate the changes of bone mineral density (BMD) and biochemical markers of bone metabolism in elderly male patients with osteoporosis (OP) . Methods BMD and bone mineral content (BMC) of the lumbar spines (L 2～4 ) and the biochemical markers of bone metabolism in serum and urine were measured. Results BMD and BMC in OP group was reduced by 21.6% and 25% respectively vs that in control group. Bone formation indicators of serum levels of ALP and BGP in OP group were increased by 25.4% and 222% respectively vs that in control. At the same time, bone resorption indicators of urine Hop/Cr and NTX/Cr ratios were significantly increased by 22.6% and 223% in OP group than that in control. Serum T level of OP group was lower than that in control group and serum 25 OH D3 was below the normal value in both groups. Conclusions BMD and BMC of the lumbar spines (L 2 4 ) are the main basis for diagnosing the osteoporosis in men. Some elder male patients with osteoporosis are due to high turnover of bone metabolism. Androgen plays an important part in maintaining the bone mass. Vitamin D deficiency in elder men is the main reason occurring osteoporosis.

Herbicides ; poisoning ; Humans ; Paraquat ; poisoning ; Poisoning ; metabolism ; pathology ; therapy

Animals ; Diet ; adverse effects ; Hyperlipidemias ; blood ; etiology ; Lipids ; blood ; Male ; Mice ; Mice, Inbred C57BL ; Physical Conditioning, Animal

Animals ; Aorta ; metabolism ; Hyperlipidemias ; metabolism ; Mice ; Mice, Inbred C57BL ; Nitric Oxide Synthase ; metabolism ; Physical Conditioning, Animal ; Physical Endurance

Objective To study the association between human papillomavirus (HPV) genotypes and viral load and clinical features of verruca vulgaris.Methods Tissue samples were collected from 48 outpatients with verruca vulgaris,and DNA was extracted from these tissue samples.To determine the genotype of HPV,PCR was performed to amplify the L1 fragment of HPV with universal primers followed by bidirectional sequencing and BLAST.The genotyping results were validated by PCR with type-specific primers.Real-time fluorescence-based quantitative PCR was conducted to measure the viral load of HPV,and hematoxylin and eosin (HE) staining to observe histological changes in these tissue specimens.Results The L1 fragment of HPV was amplified from 35 out of the 48 tissue specimens.Of the 35 L1-positive specimens,32 harbored HPV 7,1 harbored HPV 57,and 2 harbored both HPV 2 and HPV 7.Multiple lesions were observed on extremities in the patient infected with HPV 57,but on the head,face and trunk in the patients coinfected with HPV 2 and HPV 7.There were no significant differences in HPV viral load or vacuolated cell number between patients with single lesions and those with multiple lesions,or between patients with a clinical course of ＜ 6 months and those with a clinical course of 6-12 months.However,HPV viral load tended to decrease one year after the onset,and there was pronounced hyperkeratosis and less vacuolated cells in lesions of long duration (more than 2 years) compared with those of short duration (less than 2 years).Conclusions HPV 7 appears to be the most common HPV genotype associated with verruca vulgaris,and HPV 7 infection usually occurs on the head and face.For verruca vulgaris of less than 1 year,neither HPV viral load nor vacuolated cell number is associated with the count or clinical course of warts.

Objective To observe the expression of cysteine rich-protein 61 (Cyr61) on renal tubular cells,to explore its effects against hypoxic induced kidney injury and the underlying mechanisms.Methods A stably Cyr61 expressed tubular cell line Cyr61-HK2 was established based on HK2 cells and recombinant Cyr61-lentivirus.BrdU incorporation assay was used for cell proliferation.The apoptosis of cells was analyzed by flow cytometry with Annexin V and propidiumiodide staining.Western bloting was used to detect the protein expression of BAD,Akt and ERK.Results (1) Cyr61-HK2 cells displayed more proliferation ability than HK2 cells.(2) Under hypoxia condition,the apoptosis of both HK2 and Cyr61-HK2 cells increased,but the apoptosis of Cyr61-HK2 cells was lesser than HK2 cells.(3) The expression of Cyr61 led to the phosphorylation of BAD,Akt and ERK on 0 h,0.5 h,1 h (all P ＜ 0.05).Conclusion The expression of Cyr61 can promote cell proliferation and dampen cell apoptosis induced by hypoxia,which may be involved in the Akt/ERK signal pathway.

Objective To compare the effects and cost of peripheral intravenous catheter (PIV) and peripherally inserted central catheter (PICC) on very low birth weight infants (VLBWI).Methods From July 2013 to August 2015,95 VLBWI with PICC (PICC group) and 90 VLBWI with PIV (PIV group) admitted to the Neonatal Intensive Care Unit (NICU) of Changzhi Maternal and Child Care Hospital were included in the analysis.The two groups were compared in body mass increase,average length of hospital stay,incidence of catheter-related complications,and care cost and effect.Results The body mass increase per weak was higher in the PICC group than in the PIV group,with statistically significant difference (P ＜ 0.05).The average length of hospital stay in the PICC group was shorter than that in the PIV group [(48.2 ± 5.2) d vs.(53.2 ± 8.1) d,P ＜ 0.05].The incidence of catheter-related complications was lower in the PICC group than in the PIV group (35.4％ vs.44.9％),including phlebitis (PICC group,21 person-times;PIV group,169 person-times),liquid leakage or exosmose (PICC group,2 person-times;PIV group,185 person-times),and catheter prolapse (PICC group,3 person-times;PIV group,145 person-times) with statistically significant differences (all P ＜ 0.05),and catheter blockage (PICC group,7 person-times;PIV group,84 person-times) and other complications such as venous embolism and infection (PICC group,1 person-time;PIV group,3 person-times) with no statistically significant differences (all P ＞ 0.05).The average monthly cost in the PICC group (1 951.5 yuan) was lower than that in the PIV group (2 008.5 yuan),and the cost of single insertion in the PICC group (1 691.5 yuan) was higher than that in the PIV group (129.9 yuan),the cost-effectiveness was better in the PICC group than in the PIV group (30.22 vs.36.45).Conclusions For VLBWI,PICC can reduce the times of venous puncture,the incidence of complications,and promote body mass increase.However,the monthly cost was similar between the two groups in this study,possibly because of the short-time of this study.The advantages in cost-effectiveness of PICC may become more prominent when the catheter dwelling time extends.

Febrile infection -related epilepsy syndrome(FIRES)is a newly named entity of epileptic encepha-lopathy in recent years.As one of the hot spots studied by domestic and foreign scholars,the etiology and pathogenesis of FIRES,which has a poor treatment and outcome,are undefined.It manifests with refractory seizures and status epilep-ticus in previously healthy school -aged children after banal febrile illness.In addition,with the negative response to currently available treatments,there are a poor prognosis and a high mortality rate in FIRES.Survivors are typically left with refractory partial epilepsy and severe cognitive impairment.Through domestic and foreign literature,this article re-views the potential pathogenesis,clinical features,treatment and prognosis of the syndrome,and provides theoretical ba-sis and new ideas for clinical diagnosis and treatment.

AIM: To observe the effect of continuous inhaled nitric oxide (NO) on airway resistance in guinea pigs. METHODS: 36 healthy male guinea pigs were divided into control group, isoproterenol group and two inhaled NO (20?10 -6 and 60?10 -6 ) groups. Respiratory resistance (R_E) and dynamic compliance(C_ dyn )were recorded before and after evoked by histamine at different doses. RESULTS: After injections of intravenous histamine at 80,120 and 160 ?g/kg, the R_E of inhaled NO groups were apparently lower than that of control group. Compared with control group, the C_ dyn of inhaled NO groups were significantly higher after administration of histamine at 80, 120 and 160 ?g/kg. After given histamine at more than 80 ?g/kg,the R_E of inhaled NO groups were higher and the C_ dyn lower than those of isoproterenol group. CONCLUSION: Inhalation of 20?10 -6 NO and 60?10 -6 NO can inhibit the increase in airway resistance induced by higher doses (80,120 and 160 ?g/kg )of intravenous histamine, but the effect of intravenous isoproterenol seems stronger.