1.Extraction of sperm DNA from mixed stain by the modified differential lysis method combined with silicon bead method.
Hai-Jun HAN ; Yu-Hong ZHANG ; Min YANG ; Hai YI ; Geng-Ye YANG ; Dong-Tao JIA ; Da-Ru LU
Journal of Forensic Medicine 2014;30(1):50-54
OBJECTIVE:
To extract sperm DNA from mixed stain by the modified differential lysis method combined with silicon bead method and to evaluate its application value.
METHODS:
Fifty-two mixed stains containing female STR genotypes detected by differential lysis method were collected. The sperm DNA was extracted by the modified method combined with silicon bead method, then genotyped with the Identifiler Kit, and compared with the results of genotyping by the conventional differential lysis method as control.
RESULTS:
Of the 52 samples, 38 samples with sole male STR genotypes in all loci were detected. The detection rate of male STR genotypes was 98.08% through the modified method combined with silicon bead method.
CONCLUSION
The modified differential lysis method combined with silicon bead method can be used in extraction of sperm DNA from mixed stain.
DNA/isolation & purification*
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DNA Fingerprinting
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Female
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Genotype
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Humans
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Male
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Polymerase Chain Reaction
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Silicon
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Spermatozoa
2.Effects of experimental varicocele on the testosterone level in the serum and testis of rats.
Jian-jun LIU ; Qiang DONG ; Yu-ru YANG
National Journal of Andrology 2007;13(4):335-337
OBJECTIVETo study the effects of experimental varicocele (VC) on the serum testosterone (T) and intratesticular testosterone in adolescent rats.
METHODSA VC rat model was established by partial ligation of the left kidney vein in 20 SD rats, and another 20 were included in a sham operation group as controls. At 4 and 8 weeks, the concentrations of the serum T and intratesticular T were measured by radioimmunoassay (RIA). The testis tissues were homogenized and the extract liquid taken for RIA.
RESULTSCompared with the controls, the level of serum T declined at 4 and 8 weeks in the VC group, but not significantly (P > 0.05), so was that of bilateral intratesticular T at 4 weeks, and with statistical significance at 8 (P < 0.01).
CONCLUSIONWithin 8 weeks, experimental VC could reduce the level of bilateral intratesticular T, but not that of serum T. Varicocele could damage Leydig cells.
Animals ; Leydig Cells ; Male ; Rats ; Rats, Sprague-Dawley ; Testis ; metabolism ; Testosterone ; blood ; metabolism ; Varicocele ; metabolism
3.Anti-human immunodeficiency virus type 1 activity of two new nonnucleoside reverse transcriptase inhibitors in combination with other 3 antiretroviral agents in vitro
Dao-Min, ZHUANG ; Si-Yang, LIU ; Ru-Hua, DONG ; Li, BAI ; Biao, JIANG ; Xiao-Long, ZHAO ; Jing-Yun, LI
Bulletin of The Academy of Military Medical Sciences 2009;33(6):509-512
Objective:To evaluate the anti-HIV-1 activity of two new nonnucleoside reverse transcriptase inhibitors (NNRTIs), JB25 and JB26, in combination with 3 approved drugs (AZT, EFV, SQV)in vitro.Methods:The serially diluted 10 concentrations of JB25 and JB26 were combined with 7 serially diluted AZT, EFV and SQV respectively.The combination was added to 384 cell culture plates and then cocultured with HIV-1 ⅢB infected MT-2 cells for 3 days. Finally, the HIV-1 production was determined by measuring the expression of reporter genes of TZM bl cells. The data were analyzed by MacSynergy Ⅱ software.Results:The average capacity of synergism/antagonism of JB25 with AZT, EFV and SQV was 244.45/-5.05(nmol/L)~2%, 119.58/-65.93 (nmol/L)~2% and 145.83/-0.32 (nmol/L)~2% respectively;the average capacity of synergism/antagonism of JB26 with AZT, EFV and SQV was 398.90/0(nmol/L)~2%, 103.62/-0.49(nmol/L)~2% and 138.473/-0.27 (nmol/L)~2% respectively. Conclusion:Two new NNRTIs JB25 and JB26 develop synergism when combined with 3 approved drugs, respectively. MacSynergy Ⅱ software could evaluate the anti-HIV-1 activity of drug combination.
4.Determination of metanephrine and normetanephrine by high-performance liquid chromatography with electrochemical detector and its diagnostic application for pheochromocytoma
Zhe LIU ; Tao YANG ; Ying XIN ; Xiao-Dong SONG ; Yu ZHANG ; Chan-Na ZHANG ; Ru-Tai HUI ;
Chinese Journal of Laboratory Medicine 2000;0(06):-
Objective To establish high-performance liquid chromatography with electrochemical detector(HPLC-ECD) method for the determination for metanephrine and normetanephrine in 24 h urine, and provide a superior test for the diagnostic of pheochromocytomas over plasma/urine catecholamine.Methods MCX solid-phase cartridge was used for extraction of metanephrine and normetanephrine,HPLC-ECD was used for their measurements.The intra-assay CVs,interassay CVs and recoveries of metanephrine and normetanephrine were also calculated.104 hypertensive patients without pheochromocytomas and 5 pheochromocytomas patients were selected in this study.The concentrations of metanephrine and normetanephrine were compared with the plasma and 24h urinary catecholamines concentrations.Results The intra-assay CV,inter-assay CV and recovery of metanephrine were 5.9%, 7.5%,91.1% respectively;the intra-assay CV,inter-assay CV and recovery of normetanephrine were 6.3%,6.6%,88.5%,respectively.The MN,NMN,plasma CA and urine CA of all pheochromocytomas patients were positive.MN and NMN were negative in controls,while plasma CA and urine CA are false positive in 15 patients and 14 patients in controls,respectively.Conclusions The study establish a fast and accurate method for quantification of metanephrine and normetanephrine in 24 h urine by HPLC-ECD.These findings also prove that it is the best biochemical assays for pheochromocytomas at present.
5.Alterations in lymphocyte subset patterns and co-stimulatory molecules in patients with Alzheimer disease.
Shou-Ru XUE ; Dong-Hua XU ; Xin-Xin YANG ; Wan-Li DONG
Chinese Medical Journal 2009;122(12):1469-1472
Aged
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Aged, 80 and over
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Alzheimer Disease
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blood
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immunology
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B-Lymphocytes
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immunology
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B7-1 Antigen
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blood
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CD28 Antigens
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blood
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Female
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Flow Cytometry
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HLA-DR Antigens
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blood
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Humans
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Killer Cells, Natural
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immunology
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Lymphocyte Subsets
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immunology
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Male
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T-Lymphocyte Subsets
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immunology
6.Clinical experience from treatment of seven SARS patients.
Xiao-jie WANG ; Yun-ru LI ; Li-qun YANG ; Wei-yan ZHANG ; Xing-hong LI ; Qing-hua DONG ; Yu-ying YANG ; Pan XIANG ; Jie YAN ; Li-min GUO
Chinese Journal of Experimental and Clinical Virology 2004;18(3):215-217
OBJECTIVETo summarize the clinical experience from treatment of patients with severe acute respiratory syndrome (SARS).
METHODSRetrospective analysis of seven patients with SARS in Ditan hospital treated since April 22 in 2004 was performed.
RESULTSIn the 7 patients, 2 were male, 5 were female, and the average age was (35.3 plus/minus 11.3) years. The main clinical manifestations were fever, cough, minor or serious dyspnea, nausea, signs of injury to other organs, and so on. The treatment regiments included oxygen, small dosage and short period of methylprednisolone (1 to 2 mg/kg), use of ventilator, psychological intervention, and treatment of underlying diseases, after which, all the 7 patients recovered.
CONCLUSIONRational use of methylprednisolone and timely use of ventilator were the key steps of treatment.
Adult ; Anti-Inflammatory Agents ; therapeutic use ; Combined Modality Therapy ; Cross Infection ; drug therapy ; therapy ; transmission ; Female ; Humans ; Infectious Disease Transmission, Patient-to-Professional ; Male ; Methylprednisolone ; therapeutic use ; Middle Aged ; Oxygen Inhalation Therapy ; Retrospective Studies ; Severe Acute Respiratory Syndrome ; therapy ; transmission ; Ventilators, Mechanical
8. Analysis of influencing factors of heart rate deceleration capacity in patients with dilated cardiomyopathy
Fengyan WANG ; Cao ZOU ; Hongkai DONG ; Yichen YANG ; Meiwen GAO ; Ru ZHAO ; Jianling JIN ; Xiangjun YANG
Chinese Journal of Cardiology 2017;45(9):753-757
Objective:
To investigate the determinants affecting the heart rate deceleration capacity (DC) in patients with dilated cardiomyopathy (DCM).
Methods:
One hundred patients with DCM (DCM group) and 202 healthy subjects (control group) were respectively enrolled. Echocardiography and 24 hours electrocardiogram were performed in all subjects. DC value was compared between the two groups. Multiple regression analysis was made to evaluate the related determinants of DC ((age, sex, echocardiographic parameters including the left atrial diameter (LAD) and left ventricular ejection fraction (LVEF)).
Results:
(1) DC value was significantly lower in DCM group than in control group( (4.40±2.03) ms vs. (7.30±1.81) ms,
9.Therapeutic effect of human amniotic epithelial cell transplantation into the lateral ventricle of hemiparkinsonian rats.
Xin-xin YANG ; Shou-ru XUE ; Wan-li DONG ; Yan KONG
Chinese Medical Journal 2009;122(20):2449-2454
BACKGROUNDHuman amniotic epithelial cells (HAECs) are able to secrete biologically active neurotrophins such as brain-derived neurotrophic factor and neurotrophin-3, both of which exhibit trophic activities on dopamine neurons. Previous study showed that when human amniotic epithelial cells were transplanted into the striatum of 6-hydroxydopamine (6-OHDA)-induced Parkinson disease rats, the cells could survive and exert functional effects. The purpose of this study was to investigate the survival and the differentiation of human amniotic epithelial cells after being transplanted into the lateral ventricle of Parkinson's disease (PD) rats, and to investigate the effects of grafts on healing PD in models.
METHODSThe Parkinson's model was made with stereotactic microinjection of 6-hydroxydopamine (6-OHDA) into the striatum of a rat. The PD models were divided into two groups: the HAECs group and the normal saline (NS) group. Some untreated rats were taken as the control. The rotational asymmetry induced by apomorphine of the HAECs group and the NS group were measured post cell transplantation. The expression of nestin and vimentin in grafts were determined by immunohistology. Ten weeks after transplantation the density of tyrosine hydroxylase positive cells in the substantia nigra of the HAECs group, NS group and the untreated group was determined. The differentiation of grafts was determined by TH immunohistology. High performance liquid chromatography (HPLC) was used to determine monoamine neurotransmitter levels in the striatum.
RESULTSThe rotational asymmetry induced by apomorphine of the HAECs group was ameliorated significantly compared to the NS group two weeks after transplantation (P < 0.01). The grafts expressed nestin and vimentin five weeks after transplantation. TH immunohistochemistry indicated that the TH positive cells in the substantia nigra of the HAECs group increased significantly compared to the NS group (P < 0.01). Tyrosine hydroxylase (TH) positive cells in the substantia nigra of the HAEC group and the NS group were decreased compared to the untreated group (P < 0.01). Dopamine and DOPAC levels in the striatum of the HAECs group increased significantly compared to the NS group (P < 0.05). Homovanillic acid (HVA) levels in the striatum of the HAECs group increased significantly compared to the NS group (P < 0.01). In addition dopamine, DOPAC, and HVA levels in the striatum and dopamine levels in the cerebrospinal fluid of the HAECs group and the NS group were decreased compared to the untreated group (P < 0.05).
CONCLUSIONSHuman amniotic epithelial cells could be used to ameliorate the rotational asymmetry induced by apomorphine of the PD models. This could have been due to the increased content of dopamine and its metabolic products, DOPAC and HVA, in the striatum in the PD models.
Amnion ; cytology ; Animals ; Apomorphine ; pharmacology ; Chromatography, High Pressure Liquid ; Epithelial Cells ; cytology ; drug effects ; transplantation ; Female ; Homovanillic Acid ; metabolism ; Humans ; Immunohistochemistry ; Oxidopamine ; toxicity ; Parkinsonian Disorders ; chemically induced ; metabolism ; therapy ; Rats ; Rats, Sprague-Dawley
10.Hepatic iron overload in hemochromatosis: a case report.
Gen-dong YANG ; Pu-xuan LU ; Bo-ping ZHOU ; Ru-xin YE ; Jian ZANG ; Jian SU
Chinese Journal of Hepatology 2006;14(8):634-634
Hemochromatosis
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metabolism
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Humans
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Iron Overload
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Liver
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metabolism
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Male
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Middle Aged