2.Caveolin-1 expression is downregulated by shear stress and TNF-? in human aortic endothelial cells
Qing XIANG ; Xi DONG ; Mei XU ; Ruijuan SUN
Chinese Journal of Pathophysiology 1989;0(05):-
AIM: To observe effects of shear stress and TNF-? on caveolin-1 expression. METHODS: Cultured human aortic endothelial cells (HAECs) of passage 3-5 were used in the experiment. Cells were exposed to a laminar flow (shear stress 1.0 Pa) by using a parallel rectangular flow chamber for different time. Caveolin-1 mRNA and protein expression were measured by RT-PCR and Western blot, respectively. Caveolin-1 expression of the cells stimulated by TNF-? were also studied to elucidate the influence of this inflammatory factor. RESULTS: After 24 h of exposure to 1.0 Pa shear stress, both of caveolin-1 protein and mRNA expression decreased in HAECs, especially caveolin-1 mRNA expression (P
3.Advances in research of pharmacological effects and formulation studies of linalool.
Dong-mei JIANG ; Yuan ZHU ; Jiang-nan YU ; Xi-ming XU
China Journal of Chinese Materia Medica 2015;40(18):3530-3533
Linalool, as a major volatile compound, is widely distributed in natural plant essential oil. In addition, it can also be artificially synthesized. Linalool is used frequently as an important ingredient of perfumes and household detergents. It is still employed in food flavor and industries. Besides, linalool has some positive effect on healthcare. Many studies have showed that linalool exhibited a variety of pharmacological activities, including analgesic, anxiolytic, sedative, anti-inflammatory, anti-tumor and anti-bacterial effects. Therefore, linalool will be a promising agent for clinical application. This article reviews the pharmacological effects and formulation studies of linalool so as to provide a theoretical basis for its further development and utilization.
Animals
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Anti-Anxiety Agents
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chemistry
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pharmacology
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Anti-Inflammatory Agents
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chemistry
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pharmacology
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Chemistry, Pharmaceutical
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Humans
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Hypnotics and Sedatives
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chemistry
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pharmacology
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Monoterpenes
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chemistry
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pharmacology
4.Sedation technique for postoperative nasal endoscopic debridement.
Fa-jun SHENG ; Xi-bin DING ; Dong-mei CHEN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2006;41(2):143-144
Adolescent
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Adult
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Debridement
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methods
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Endoscopy
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Female
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Humans
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Male
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Middle Aged
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Neuroleptanalgesia
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methods
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Nose
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surgery
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Postoperative Period
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Young Adult
5.Application of Response Surface Analysis (RSA) in Fermentation Condition Optimization of Penicillium decumbens Ju-A_(10) for CMCase Production
Xi-Wen DONG ; Chun-Mei DU ; Jian-Qiang LIN ; Yin-Bo QU ;
Microbiology 1992;0(03):-
Fermentation condition optimization of P. decumbens Ju-A10 for production of CMCase using three kinds of plant cellulosic wastes as carbon sources was made using RSA method. The result was that CMCase was the highest when the level of carbon source was 9. 77 % , 8. 69 % and 9. 97% , and liquid volume was 64. 7 mL, 54. 2 mL, 40. 8 mL for carbon sources of millet straw, wheat straw and paper sludge, respectively. The value of CMCase was 29. 26IU/mL, 29. 14 IU/mL, 29. 81 IU/mL, respectively, in the above cases. The value of R2 is 0. 9117 , 0. 9246, 0. 8655 , respectively. It could be concluded that the fermentation models were quite reliable. The method can be applied in optimization of fungi fermentation medium.
6.Progress in Solid-state Nanopore-based Analysis Technology
Zhi LI ; Li-Ping LIU ; Zhen FANG ; Dong-Mei XI
Chinese Journal of Analytical Chemistry 2018;46(6):836-842
Nanopore technique is a low-cost tool for single-molecule level analysis without the need of label or amplification. The solid nanopores have been widely used in many fields such as chemistry and life sciences due to their advantages such as high stability, good tolerability, controllable size, and easy for modification. The solid nanopores commonly used in recent years are fabricated using two types of materials: membrane and tube. The membrane materials include silicon nitride, two-dimensional materials, aluminium oxide, and polymer membranes. The tube materials mainly include glass capillary and carbon nanotube. This review summarizes and prospects the research progress of different solid nanopores.
7.Secular trend of nosocomial pneumonia in an university hospital in Zhengzhou.
Dong-sheng HU ; Qiu-ping FAN ; Hua-yan XING ; Hui-qin ZHANG ; Yuan-lin XI ; Mei-xi ZHANG ; Wei-dong ZHANG
Chinese Journal of Preventive Medicine 2006;40(1):29-32
OBJECTIVETo investigate the secular trend of infection rate, risk factor exposure rates for nosocomial pneumonia (NP), and to evaluate the nosocomial infection surveillance and control programs efficacy in an university hospital from 1993 to 2000.
METHODSAll 126 665 hospitalized patients from 1993 to 2000 were studied for NP. The independent risk factors for NP were analyzed by using case-control study method and logistic regression technique. The time-specific rates for NP and risk factor exposure were calculated annually.
RESULTSThe infection rates for NP were decreased by 50% from 1.20% in 1993 to 0.60% in 2000. The logistic regression analysis showed that the independent risk factors for NP were immunosuppressive therapy (OR = 2.72), chemotherapy (OR = 2.17), cancer (OR = 1.45), chronic obstructive pulmonary disease (COPD, OR = 1.88), ICU (OR = 3.18), coma (OR = 3.26), tracheotomy (OR = 14.95), hemodialysis (OR = 5.12), bone or lumbar puncture (OR = 1.82). The time-trends for exposure rates of COPD and bone or lumbar puncture were slightly decreased, however those for the others and the synthetic risk factors were not changed significantly.
CONCLUSIONThe infection rates for NP were significantly decreased in the case of no change for exposure rates of risk factors for NP, this suggests that the nosocomial infection surveillance and control programs were effective for lowering infection rate for NP in this hospital.
China ; epidemiology ; Cross Infection ; epidemiology ; prevention & control ; Hospitals, University ; statistics & numerical data ; Humans ; Infection Control ; methods ; Inpatients ; statistics & numerical data ; Prospective Studies ; Reproducibility of Results ; Respiratory Tract Infections ; epidemiology ; prevention & control ; Risk Factors ; Time Factors
8.Factors associated with acute febrile reaction in elderly patients receiving intravenous zoledronic acid for osteoporosis.
Hai-ou DENG ; Dong-feng LI ; Wei-jie ZHANG ; Xi-mei ZHI ; Ling XU ; Wen WU
Journal of Southern Medical University 2011;31(12):2076-2078
OBJECTIVETo survey the incidence of acute febrile reaction in elderly patients receiving intravenous zoledronic acid for osteoporosis and identify the related factors.
METHODSThirty-eight elderly patients with osteoporosis were hospitalized and treated with intravenous infusion of 5 mg zoledronic acid in 2010. The incidence of acute fever reaction was observed in these patients , and the time of fever onset, duration, average maximum temperature, and antipyretic drug used were recorded. The patients with and without acute febrile reaction were compared for age, duration of osteoporosis, sex ratio, use of parathyroid hormone before zoledronic acid treatment, β-fragment of collagen breakdown, calcitonin, osteocalcin, serum calcium, and use of anti-osteoporosis drugs before the treatment.
RESULTSAcute fever reaction occurred in 12 (31.6%) of the patients. Two of the patients had fever on the day of zoledronic acid treatment, and the other patients developed fever after the first day of treatment, with a mean duration of 1 day and a maximum temperature of (38.5∓0.84) degrees celsius;. The fever was treated with a mean of 3.55∓1.21 pseudoephedrine tablets. The patients with fever showed significantly higher parathyroid hormone levels before treatment than those without fever (P<0.05); osteocalcin, calcitonin, β-fragment of collagen breakdown, or serum calcium showed no significant difference between the two groups.
CONCLUSIONAcute febrile reaction, often moderate and transient, is common in elderly patients receiving intravenous zoledronic acid for osteoporosis, and its occurrence is possibly associated with parathyroid hormone levels before the treatment.
Aged ; Aged, 80 and over ; Bone Density Conservation Agents ; administration & dosage ; adverse effects ; China ; epidemiology ; Diphosphonates ; administration & dosage ; adverse effects ; Female ; Fever ; chemically induced ; Humans ; Imidazoles ; administration & dosage ; adverse effects ; Incidence ; Infusions, Intravenous ; Male ; Osteoporosis ; drug therapy ; Parathyroid Hormone ; blood
9.Influence of chemotherapy on expression of TRAIL in primary acute leukemic cells.
Yan-Fang LIU ; Sheng-Mei CHEN ; Hui SUN ; Jian-Xi DONG ; Qiu-Tang ZHANG
Journal of Experimental Hematology 2006;14(3):481-484
In order to explore the expression of TRAIL in primary acute leukemic cells and the effect of chemotherapeutic drug on TRAIL expression in acute leukemic cells, the expression of TRAIL was assessed by flow cytometry on day 0, day 1, day 3 and day 5 in 16 patients with acute leukemia received chemotherapy. Meanwhile, the bone marrow mononuclear cells of acute leukemia patients were cultured in vitro with VP-16 and INFalpha-2a. Expression of TRAIL was analyszed by flow cytometry at 24, 48 and 72 hours after treatment. The results showed that the expression of TRAIL in the peripheral blood mononuclear cells was upregulated significantly from day 1 after chemotherapy (P < 0.05). In in vitro culture test, VP-16 upregulated the expression of TRAIL on acute leukemia bone marrow mononuclear cells (P < 0.05). Compared with VP-16 alone, the combination of VP-16 with IFNalpha-2a showed no synergic effects on the expression of TRAIL. It is concluded that the expression of TRAIL increases after chemotherapy in vivo and after treatment with VP-16 and IFN in vitro, which suggests that the apoptosis induced by TRAIL may play an important role in chemotherapy of leukemia.
Acute Disease
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Antineoplastic Agents
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pharmacology
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therapeutic use
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Bone Marrow Cells
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metabolism
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Etoposide
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pharmacology
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Humans
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Interferon-alpha
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pharmacology
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Leukemia
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drug therapy
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metabolism
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pathology
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TNF-Related Apoptosis-Inducing Ligand
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biosynthesis
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genetics
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Tumor Cells, Cultured
10.Analysis of viral etiology of severe pneumonia in infants and young children in Chongqing area.
Chun-mei YU ; Xi-qiang YANG ; Feng XU ; Ze-lan ZUO ; Xiao-dong ZHAO
Chinese Journal of Pediatrics 2010;48(2):143-147
OBJECTIVETo investigate the prevalence of viral infections and putative association of viral infection with illness severity in young children with severe lower respiratory tract infection (LRTI) in Chongqing.
METHODRespiratory secretion specimens were collected from 119 hospitalized patients with severe pneumonia from December 2006 to March 2008.After being processed, the samples were detected for respiratory viruses including respiratory syncytial virus (RSV), adenovirus (ADV), human metapneumovirus (hMPV), human bocavirus (HBoV), parainfluenza virus 1, 2, 3 (PIV 1, 2, 3), influenza virus A and B (IVA and IVB) either by PCR or RT-PCR. Clinical data were analyzed along with virological data by using appropriate statistical methods.
RESULTViral pathogens were identified in specimens of 86 (72.3%) cases, among which RSV was detected in 49 (41.2%) patients. More than one virus was detected in 23 individual (26.7%) samples, of which 19 were dual positive for RSV and another virus. Bacterial cultures were performed for 69 patients. Both bacterial and viral pathogens were identified in 53 (76.8%) patients. Bacterial and viral coinfection was demonstrated in samples from 41 (59.4%) cases.
CONCLUSIONViral pathogens are the main etiology of severe pneumonia in young children in Chongqing area during the study period. RSV was the most frequent viral pathogens, followed by ADV and hMPV. Coinfection with respiratory common viruses was relatively common, though co-infection with viruses did not appear to aggravate the patients' condition.
Adenoviridae ; isolation & purification ; Child, Preschool ; China ; epidemiology ; Human bocavirus ; isolation & purification ; Humans ; Infant ; Infant, Newborn ; Influenza A virus ; isolation & purification ; Metapneumovirus ; isolation & purification ; Pneumonia, Bacterial ; microbiology ; virology ; Pneumonia, Viral ; microbiology ; virology ; Respiratory Syncytial Viruses ; isolation & purification ; Virus Diseases ; virology