2.Caveolin-1 expression is downregulated by shear stress and TNF-? in human aortic endothelial cells
Qing XIANG ; Xi DONG ; Mei XU ; Ruijuan SUN
Chinese Journal of Pathophysiology 1989;0(05):-
AIM: To observe effects of shear stress and TNF-? on caveolin-1 expression. METHODS: Cultured human aortic endothelial cells (HAECs) of passage 3-5 were used in the experiment. Cells were exposed to a laminar flow (shear stress 1.0 Pa) by using a parallel rectangular flow chamber for different time. Caveolin-1 mRNA and protein expression were measured by RT-PCR and Western blot, respectively. Caveolin-1 expression of the cells stimulated by TNF-? were also studied to elucidate the influence of this inflammatory factor. RESULTS: After 24 h of exposure to 1.0 Pa shear stress, both of caveolin-1 protein and mRNA expression decreased in HAECs, especially caveolin-1 mRNA expression (P
3.Advances in research of pharmacological effects and formulation studies of linalool.
Dong-mei JIANG ; Yuan ZHU ; Jiang-nan YU ; Xi-ming XU
China Journal of Chinese Materia Medica 2015;40(18):3530-3533
Linalool, as a major volatile compound, is widely distributed in natural plant essential oil. In addition, it can also be artificially synthesized. Linalool is used frequently as an important ingredient of perfumes and household detergents. It is still employed in food flavor and industries. Besides, linalool has some positive effect on healthcare. Many studies have showed that linalool exhibited a variety of pharmacological activities, including analgesic, anxiolytic, sedative, anti-inflammatory, anti-tumor and anti-bacterial effects. Therefore, linalool will be a promising agent for clinical application. This article reviews the pharmacological effects and formulation studies of linalool so as to provide a theoretical basis for its further development and utilization.
Animals
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Anti-Anxiety Agents
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chemistry
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pharmacology
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Anti-Inflammatory Agents
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chemistry
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pharmacology
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Chemistry, Pharmaceutical
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Humans
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Hypnotics and Sedatives
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chemistry
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pharmacology
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Monoterpenes
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chemistry
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pharmacology
4.Sedation technique for postoperative nasal endoscopic debridement.
Fa-jun SHENG ; Xi-bin DING ; Dong-mei CHEN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2006;41(2):143-144
Adolescent
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Adult
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Debridement
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methods
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Endoscopy
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Female
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Humans
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Male
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Middle Aged
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Neuroleptanalgesia
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methods
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Nose
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surgery
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Postoperative Period
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Young Adult
5.Application of Response Surface Analysis (RSA) in Fermentation Condition Optimization of Penicillium decumbens Ju-A_(10) for CMCase Production
Xi-Wen DONG ; Chun-Mei DU ; Jian-Qiang LIN ; Yin-Bo QU ;
Microbiology 1992;0(03):-
Fermentation condition optimization of P. decumbens Ju-A10 for production of CMCase using three kinds of plant cellulosic wastes as carbon sources was made using RSA method. The result was that CMCase was the highest when the level of carbon source was 9. 77 % , 8. 69 % and 9. 97% , and liquid volume was 64. 7 mL, 54. 2 mL, 40. 8 mL for carbon sources of millet straw, wheat straw and paper sludge, respectively. The value of CMCase was 29. 26IU/mL, 29. 14 IU/mL, 29. 81 IU/mL, respectively, in the above cases. The value of R2 is 0. 9117 , 0. 9246, 0. 8655 , respectively. It could be concluded that the fermentation models were quite reliable. The method can be applied in optimization of fungi fermentation medium.
6.Survey of attitude and knowledge of pulmonary tuberculosis among grade A hospital nurses
Ming-Xia XI ; Dong-Mei HUANG ; Li-Min QING
Chinese Journal of Modern Nursing 2012;18(17):1993-1996
Objective To study the attitude and knowledge of pulmonary tuberculosis among clinical nurses,so as to provide reference evidence for launching targeted tuberculosis special training.Methods Convenience sampling was adopted to investigate 375 nurses in Grade A hospital with tuberculosis questionnaire.Results The average score of related knowledge of pulmonary tuberculosis among clinical nurses was ( 18.71 ±3.45) with a medium level,and the dimensions score of protection measure,medicine control was lower and respectively was (3.88 ± 1.25 ) and (4.15 ± 1.11 ).The average score of attitude among nurses was (40.08 ±6.01) with a medium level.The tuberculosis nursing experience,attitude and titles were the important influencing factors of knowledge score ( P< 0.05 ),and the attitude of pulmonary tuberculosis was positively correlated with knowledge and training(r =0.187,0.122,respectively ;P <0.05 ).Conclusions The knowledge of pulmonary tuberculosis among nurses is incomprehensive,ss it is important to strengthen the training on the knowledge of tuberculosis and improve nurses' attitude for the tuberculosis patients,and fully play their role in the prevention and treatment of the pulmonary tuberculosis.
7.Progress in Solid-state Nanopore-based Analysis Technology
Zhi LI ; Li-Ping LIU ; Zhen FANG ; Dong-Mei XI
Chinese Journal of Analytical Chemistry 2018;46(6):836-842
Nanopore technique is a low-cost tool for single-molecule level analysis without the need of label or amplification. The solid nanopores have been widely used in many fields such as chemistry and life sciences due to their advantages such as high stability, good tolerability, controllable size, and easy for modification. The solid nanopores commonly used in recent years are fabricated using two types of materials: membrane and tube. The membrane materials include silicon nitride, two-dimensional materials, aluminium oxide, and polymer membranes. The tube materials mainly include glass capillary and carbon nanotube. This review summarizes and prospects the research progress of different solid nanopores.
8.Secular trend of nosocomial pneumonia in an university hospital in Zhengzhou.
Dong-sheng HU ; Qiu-ping FAN ; Hua-yan XING ; Hui-qin ZHANG ; Yuan-lin XI ; Mei-xi ZHANG ; Wei-dong ZHANG
Chinese Journal of Preventive Medicine 2006;40(1):29-32
OBJECTIVETo investigate the secular trend of infection rate, risk factor exposure rates for nosocomial pneumonia (NP), and to evaluate the nosocomial infection surveillance and control programs efficacy in an university hospital from 1993 to 2000.
METHODSAll 126 665 hospitalized patients from 1993 to 2000 were studied for NP. The independent risk factors for NP were analyzed by using case-control study method and logistic regression technique. The time-specific rates for NP and risk factor exposure were calculated annually.
RESULTSThe infection rates for NP were decreased by 50% from 1.20% in 1993 to 0.60% in 2000. The logistic regression analysis showed that the independent risk factors for NP were immunosuppressive therapy (OR = 2.72), chemotherapy (OR = 2.17), cancer (OR = 1.45), chronic obstructive pulmonary disease (COPD, OR = 1.88), ICU (OR = 3.18), coma (OR = 3.26), tracheotomy (OR = 14.95), hemodialysis (OR = 5.12), bone or lumbar puncture (OR = 1.82). The time-trends for exposure rates of COPD and bone or lumbar puncture were slightly decreased, however those for the others and the synthetic risk factors were not changed significantly.
CONCLUSIONThe infection rates for NP were significantly decreased in the case of no change for exposure rates of risk factors for NP, this suggests that the nosocomial infection surveillance and control programs were effective for lowering infection rate for NP in this hospital.
China ; epidemiology ; Cross Infection ; epidemiology ; prevention & control ; Hospitals, University ; statistics & numerical data ; Humans ; Infection Control ; methods ; Inpatients ; statistics & numerical data ; Prospective Studies ; Reproducibility of Results ; Respiratory Tract Infections ; epidemiology ; prevention & control ; Risk Factors ; Time Factors
10.Inhibiting effect of adenosine on platelet activation in vitro.
Dong-Mei WANG ; Jing-Han LIU ; Jun ZHOU ; Xi-Lin OUYANG ; Yan-Chao XING
Journal of Experimental Hematology 2005;13(6):1094-1098
This study was aimed to investigate the inhibiting effects of adenosine on platelet in vitro in order to select functional protectants for platelets before lyophilization. Platelet membrane surface CD62P expression was assayed by flow cytometer (FCM). Platelet aggregations induced by restocetin, thrombin, ADP and propyl gallate were detected by APACT-2. The results showed that platelets membrane surface CD62P expression increased significantly after pre-treating of freeze-drying. 0.75 mmol/L adenosine could inhibit CD62P expression in a dose-dependent manner. Adenosine could inhibit platelet aggregation induced by propyl gallate, but no action on restocetin. When adenosine concentration was 1.0 mmol/L or higher, the aggregation induced by thrombin was significantly restrained. When concentration of adenosine was 0.75 mmol/L, platelet activation resulted from retreating could be inhibited and platelet aggregation induced by restocetin and thrombin were not affected markedly. It is concluded that adenosine can be one of the functional protectants and activation inhibitors in vitro for platelet cryo-preservation.
Adenosine
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pharmacology
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Blood Platelets
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drug effects
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metabolism
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Blood Preservation
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Cryopreservation
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Flow Cytometry
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Humans
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P-Selectin
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analysis
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Platelet Activation
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drug effects
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Platelet Aggregation
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drug effects
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Platelet Aggregation Inhibitors
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pharmacology