Objective To investigate the clinical significance of urine transferrin in the type 2 diabetic nephropathy and primary chronic glomerular disease. Methods The level of TRF and UAER in the urine of 114 patients with type 2 diabetes mellitus (T2DM), 108 patients with CGD and 30 healthy patients was measured by rate nephelometry and analyzed. Results Urine TRF significantly increased earlier than ALB at the early stage of DN; Linear correlation was found by correlation analysis between the level of TRF in urine level and the quantity of protein in urine. Conclusion TRF in urine obviously increased at the early stage of DN. It was more sensitive than UAER to reflect the early renal injury of DM. A statistically significant correlation was found between the TRF level and the quantity of protein in the urine.

Objective To study the inhibitory effect and its mechanism of sodium butyrate on human laryngeal carcinoma in nude mice. Methods Human laryngeal carcinoma cell line Hep-2 was seeded in the subcutaneous layer of 12 nude mice to built laryngeal carcinoma xenograft model. Then they were randomly and equally divided into 2 groups. Sodium butyrate was given in experimental group while phosphatic-buffered saline (PBS) was used in control group for 4 weeks. Tumor size and body weight of the mice were measured at regular time-intervals. The tumor,heart,liver,lungs,spleen and kidneys were removed at the end of treatment. Tumor sections were examined by electronic microscopy. TUNEL method and immunohistochemical S-P method were used for detecting the expression of Ki-67 nuclear antigen and survivin protein. The heart,liver,lung,spleen and kidney sections were examined after HE staining for assessment of toxicity. Results In experimental group,the volume of tumors was reduced,the area of necrosis in tumors was widened,the apoptotic rate was increased obviously and the expression level of Ki-67 nuclear antigen and survivin protein was decreased as compared with control group. During treatment,all the nude mice grew well and there were no toxic reactions. At the end of treatment,there were no abnormal changes in heart,liver,lung,spleen and kidney sections examined under light microscope. Conclusion Sodium butyrate can significantly inhibit the growth of human laryngeal carcinoma xenograft in nude mice. Its mechanism may be related to the apoptosis in tumor cells by inhibiting the expression of survivin protein and Ki-67 nuclear antigen. There is no toxicity to heart,liver,lungs,spleen and kidneys at a treatment dose of sodium butyrate.

0 05), there had slight interference to low value serum and had no interference to high value serum by 125 mg/L Vitamin C, the correlation of UIBC reagent kit from different manufacturer was excellent( r = 0 994 4, n = 198) UIBC of serum samples from100 health blood donors (19 years old to 52 years old) were determined with UIBC reagent kit, and the reference result is 26 0-51 0 ?mol/L( ? 2 s ) Conclusion The UIBC result determined by this method is exact and reliable The UIBC reagent kit is easy to operate and ready for automated analyzer Then UIBC assay is worth to popularize

Objective To explore the DNA damage in peripheral blood lymphocytes of rats exposed to sodium arsenite. Methods Thirty-two Wistar rats, weighing 180 - 200 g, equal male and female, were randomly divided into 4 groups, 8 in each group. Sodium arsenite 0(control) ,0.05,0.15,0.45 mg/L were given through drinking water for 30 days. Body weight and drinking water consumption were measured every day. Blood were collected and DNA damage in peripheral blood lymphocytes was examined by single cell gel electrophoresis.Results The increase of body mass[( 121.00 ± 38.57), ( 120.62 ± 42.80), ( 125.38 ± 48.68)g]and water intake [(36.9 ± 6.2), (37.9 ± 5.8), (39.3 ± 4.2)ml/d]in 0.05,0.15,0.45 mg/L sodium arsenite groups were compared with the control group[( 119.25 ± 47.27)g, (38.4 ± 5.1 )ml/d], and the difference were not significant (F = 0.040,0.828, all P ＞ 0.05). The tail ratios[46.25%(185/400) ,57.00%(228/400),64.00%(256/400)], tail lengths [(32.89 ± 17.18), (58.74 ± 36.28), (77.55 ± 35.73 ) μm]and tail moments [(6.29 ± 3.74), ( 11.20 ± 9.64),(17.30 ± 12.60)μm]in 0.05,0.15,0.45 mg/L sodium arsenite groups were significantly higher than those of the control group[39.25%(157/400), (18.73 ± 15.83),(2.61 ± 1.05)μm, all P ＜ 0.01], and the tail ratios,tail lengths and tail moments in lymphocytes increased with increased doses of arsenic concentration. Conclusions Low doses of arsenic exposure can induce DNA damage in peripheral blood lymphocytes of rats.

Objective To investigate the expression and significance of death receptor 4(DR4) and DR5 in human craniopharyngioma.Methods The expression of DR4 and DR5 was determined by immunohistochemistry and in situ hybridization in 28 samples of craniopharyngioma and 25 samples of normal brain tissue.Results With low expression in partial normal brain tissue,DR was expressed highly in all of the craniopharyngioma samples.High DR expression in craniopharyngioma tissue differed from low DR expression in normal brain tissue(P0.05).Conclusions High DR expression is prevalent in craniopharyngioma tissue.This may contribute to the apoptosis-induced therapy of craniopharyngioma.The control of DR expression lays in protein level.This may contribute to the selective induced-apoptosis of tumor necrosis factor-related apoptosis-induced ligand.

BACKGROUNDCoronary artery lesions (CALs) are known to be the main complication in children with Kawasaki disease (KD). Instead of intravenous immunoglobulin (IVIG), corticosteroid therapy has been accepted to be used for children with KD who are unresponsive to IVIG. This study aimed to evaluate risk factors for CALs in children with KD.

METHODSWe retrospectively reviewed the clinical records of 2331 children with KD from January 2005 to December 2014. To identify the independent risk factors for CALs, multivariable logistic regression models were constructed using significant variables identified from univariate logistic regression analysis.

RESULTSThe incidence of CALs was 36.0% (840 of 2331), including 625 (26.8%) coronary artery dilations and 215 (9.2%) coronary artery aneurysms (CAAs). Multivariable logistic regression analysis identified that male, incomplete KD, longer fever duration, and C-reactive protein (CRP) >100 mg/L were independent risk factors for coronary artery dilatations. On the other hand, male, incomplete KD, longer fever duration, prolonged days of illness at the initial treatment, corticosteroid therapy, sodium ≤133 mmol/L, and albumin <35 g/L were the independent risk factors for CAAs. In addition, corticosteroid therapy, prolonged days of illness at the initial treatment, and albumin <35 g/L were the independent risk factors for giant CAAs.

CONCLUSIONSCALs might be associated with male sex, incomplete KD, longer fever duration, prolonged days of illness at the initial treatment, albumin <35 g/L, sodium ≤133 mmol/L, CRP >100 mg/L, and corticosteroid therapy. Corticosteroid therapy was an independent risk factor for CAAs and giant CAAs. Thus, corticosteroids should be used with caution in the treatment of KD with the risk for CALs.

Adolescent ; Adrenal Cortex Hormones ; adverse effects ; Child, Preschool ; Coronary Aneurysm ; chemically induced ; Female ; Humans ; Infant ; Logistic Models ; Male ; Mucocutaneous Lymph Node Syndrome ; drug therapy ; Retrospective Studies

Based on introducing knowledge graph, the paper discusses how to construct Traditional Chinese Medicine ( TCM ) knowledge graph from the aspects of data sources, research contents and visualized display, so as to integrate TCM knowledge resources. It also puts forward the application prospect.

Objective To investigate the effects of signal transducer and activator of transcription 3 (STAT3) RNAi on the content of reactive oxygen species (ROS) and the DNA damage in glioma cells.Methods Glioma cells of the line U251 cells were cultured and transfected with STAT3 RNAi plasmid (pSilencer2.1-STAT3,STAT3 group) and pSilencer2.1-GFP (GFP control group) respectively.Part of the U251 cells were irradiated with γ-rays of 60Co as positive control group of smear phenomenon.The levels of ROS and malondialdehyde (MDA) in the cells were detected 24,48,and 72 h later by flow cytometry and fluorescence chamoluminescence analyzer,respectively.The DNA damage in the transfected U251 cells was examined by using single cell gel electrophoresis assay,and the cell cycle distribution was examined using FACS PI staining 12,24,and 36 h later.Results At 24 h after the transfection,the ROS level of the siSTAT3-transfected ceils was 8.91 times that of the control group (F = 89.296,P < 0.05),and returned to the normal level 48 h later.There were not significant differences in the MDA level of the cells 24,48,and 72 h later between the siSTAT3 group and siGFP group.Compared with the 8 Gy irradiation positive group with obvious smear phenomenon,smear phenomenon was shown in part of the ceils in the siSTAT3 group 6 h later,became less 12 h later,and disappeared completely 24 h later.Compared with the control group,lag of S stage rate was 17.22% and the lag of G2/M stage rate was 6.4% 12 h later in the siSTAT-transfected group,and the G0/G1 stage lag rate was 18.44% 24 h later,and the lag of S stage rate was 17.99% 36 h later.Conclusions Inhibition of STAT3 results in the change of oxidoreduction status in glioma cells,as well as damage and reparation of DNA.

Objective To investigate the effect of ionizing radiation on the invasion of the pulmonary adenocarcinoma cell line A549.Methods The invasiveness of A549 cells irradiated with 2 and 4 Gy doses of γ-rays was detected by using transwell invasion assay.The expression levels of matrix metalloproteinase (MMP)-2 mRNA and protein and phosphorylated signal transducers and activators of transcription 3 ( STAT3 ) protein were detected by reverse transcription PCR and Western blot.Results After irradiation with 2 or 4 Gy, the invasiveness of A549 cells increased by 200.0% ( F = 111.7, P ＜ 0.01 ) and 390.9% ( F = 593.7, P ＜ 0.01 ), respectively, compared with that in untreated A549 cells.Furthermore, the transcription and protein expression of MMP-2 24 h after irradiation and the phosphorylation of STAT3 12 h after irradiation were promoted.The irradiation-induced elevation of MMP-2 protein expression was suppressed using STAT3 phosphorylation specific inhibitor (AG490).Moreover,compared with 4 Gy of irradiation alone, treatment with 4 Gy of irradiation plus AG490 decreased the number of invasive cells by 76.1% ( F = 555.9, P ＜ 0.01 ), and the number of invasive cells in 4 Gy of irradiation plus AG490 group made up only 117.8% of that in untreated group ( F = 3.6, P ＞ 0.05 ).Conclusions Ionizing radiation could activate STAT3, which triggers the transcription of MMP-2, and then promote the invasiveness of A549 cells.

Objective To compare the event-related potential(ERP) difference waveforms between attention deficit hyperactivity disorder (ADHD) children and normal children in the conflict task,and to investigate the neural mechanism of conflict monitoring of ADHD. Methods ERPs elicited by performing the spatial SimonStroop task were recorded in 32 children with ADHD and 32 normal children. The differences of ERP and behavior dren with ADHD(0.80±0. 11 ,0.87 ±0.08) were less than normal children (0.88 ±0.07,0.93 ±0. 04)in Sireaction time of ADHD group( (773.03 ±99.34)ms) in the Siin were longer than the control group( (737.21 ±81.40)ms) ,the differences had statistic significance (P＜0. 05). 2. ERP results:the amplitude of N2 of the children with ADHD in the four conditions were smaller than the control group, the difference in Siin had statistic sigof prefrontal and anterior cingulate cortex of ADHD were weaker than the control group. Conclusions The results suggest that, due to the inadequate allocation of attention resources of the children with ADHD, they have conflict monitoring function defects compare with normal children. The function defects of conflict information processing related brain areas may be the main neural mechanism. The result of the behavior and ERP can become the reference indicator for the early diagnosis and treatment of ADHD.