OBJECTIVE:To evaluate therapuetic efficacy and toxic reaction of 2 therapuetic regimens for 56 cases of bone me-tastasis of cervical cancer. METHODS:56 cases of bone metastasis of cervical cancer with complete information were collected ret-rospectively and randomly divided into radiochemotherapy group(31 cases)and diphosphonate group(25 cases). Radiochemothera-py group was given radiotherapy,chemotherapy and diphosphonate;diphosphonate group was given palliative symptomatic treat-ment of diphosphonate;a treatment course lasted for 3-4 weeks,and both groups received 3 courses of treatment. Average survival time,survival quality and toxic reaction were comparison in 2 groups. RESULTS:After bone metastasis of cervical cancer,the av-erage survival time of radiochemotherapy group was 17 months (95%CI:9.1-24.9),and that of diphosphonate group was 19 months (95%CI:2.4-35.6);3 years later,the survival rate of radiochemotherapy group was 16.1%,and that of diphosphonate group was 16.0%,there was no statistical significance(P=0.820). To compared KPS after 3 treatment courses,the improvement rate of radiochemotherapy group(22.6%)was significantly lower than that of diphosphonate group(40.0%),with statistical signif-icance (χ2=4.36,P<0.05). Toxic reaction of radiochemotherapy group was more significant than that of diphosphonate group. CONCLUSIONS:Diphosphonate has little effect on survial quality and slight toxic reaction. It is worthy of spreading in some pa-tients with advanced,elderly and reccurred bone metastasis of cervical cancer.

0.05).CD34 showed widespread expression in cholangio-carcinoma tissues,but limited in normal bile duct,which showed significant difference(P

Objective To establish content determination of hydroxyl safflower yellow A in Biyangqing.Methods Hhgh-performance liquid chromatography(HPLC)was used in the determination.A C18 column was used for the separation flow rate Was set at 1.0mL/min,the temperature of the column was set at 30℃,and wavelength of diction was set at 403 nm.with 100.08%average recovery and 0.98%RSD.Conclusion This detrmination method is specific and reproducible and can be used to control the quality of Biyangqing.

Objective To investigate the relationship between human epidermal growth factor receptor 2 (HER-2) gene amplification, the expression of HER-2 protein and the clinical characteristics in invasive breast cancer. Methods 105 patients with invasive breast cancer were examined by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). The correlation between expression of HER-2 protein and the gene amplification of HER-2 was compared, and their relationship with clinicopathologic features was analyzed. Results 105 patients with invasive breast cancer included 35 cases of HER-2 gene amplification. The coincidence rates of HER-2 protein 0 and + (negative), ++ (uncertainty),+++ (positive) and FISH detection of HER-2 gene amplification were 94.5 % (17/18), 23.5 % (16/68) and 94.7 % (18/19) respectively. HER-2 gene amplification was associated with estrogen receptor, progesterone receptor expression and lymph node metastasis (all P< 0.05), but not with age and tumor differentiation (P>0.05). Conclusions The prognosis of invasive breast cancer is correlated with the amplification of HER-2 gene. Patients with HER-2 protein negative and positive can be selectively confirmed by FISH; patients with HER-2 protein uncertainty should routinely use FISH to detect HER-2 gene amplification status, which could provide data for the clinical treatment and prognosis judgement.

Considering a variety of theories such like Dunkin's, Centra's and Kolitch's, a set of 13 indicators are designed to evaluate effect of courses taken in high education of China currently, compro-mised by chief aspects of course designing, practicing, and effect. Three courses including medical nursing, humanistic cultivation and communication skills in nursing and fundamentals of nursing in a nursing de-partment are chosen to be objects of questionnaire survey about the above indicators. The results show that there are significant differences in curriculum effectiveness between different courses. The scores are 2.81, 2.80 and 2.60 respectively, and teachers and students have different evaluation on the same indicators of the same curriculum,which provides a reference for teachers, colleges and universities and teaching management departments to promote the improvement of teaching quality.

Objective To investigate the Influence of Survivin and hTERT gene on cell proliferation and apoptosis in human colorectal carcinoma cell line SW 480 and to find experiment evidence for gene therapy of colorectal carci-noma .Methods Plasmids carrying shRNAs targeting survivin and hTERT were designed , constructed and trans-fected into SW480 cells.SW480 cells were then divided into blank group , blank Plasmid control group , survivin RNAi group , hTERT RNAi group and Survivin-hTERT RNAi group .The telomerase activity was examined by TRAP-PCR-ELISA analysis 48h after hTERT-shRNA transfection.Survivin and hTERT mRNA and protein expres-sion was analyzed by RT-PCR and Western blot .Cell apoptosis , proliferation were measured by flow cytometry , CCK-8 assay.Results Telomerase activity of SW480 cells in Survivin-hTERT RNAi groups were significantly decreased compared with the blank group ( P<0.01 ) .The expression of survivin and hTERT mRNA, proteins in the Survivin-hTERT RNAi group was reduced by 82.8%and 73.6%( P<0.01 ) ,79.2%and 66.7%( P<0.01 ) respectively .The inhibitory rate of cell proliferation of Survivin-hTERT RNAi group was 43.6% ±0.1%( P <0.01 ) .The apoptosis rate was 39.2%±2.3%( P<0.01 ) in the Survivin-hTERT RNAi group .Conclusions The Survivin-hTERT RNAi group could significantly reduces the protein expression of survivin and hTERT mRNA, in-hibit cell proliferation and induces cell apoptosis in human colorectal carcinoma cell line SW 480 .

Objective To explore the clinical curative effect of hollow screw internal fixation for the treatment of distal epiphyseal teenagers tibial fracture.Methods Thirty -nine cases of epiphyseal fractures of the distal tibia were treated in Shanghai Children′s Hospital between February 2005 and September 201 4.This study included 24 boys and 1 5 girls with the average age of 1 3.1 years (ranged 1 0.5 to 1 6.2 years old).Preoperative diagnosis was confirmed by the X -ray films or CT examination.All the cases were closed fractures and manipulative reduction all failed.Surgi-cal reduction was performed when plain radiographs showed the fracture gap of epiphysis board and articular surface were greater than 2 mm or after closed reduction.Under C -arm X -ray machine,ankle axial traction was performed initially.Anatomical reduction underwent according to the bone fracture type.The fractures were fixed with cannulated screws by percutaneous or open approach.Postoperative X -ray or CT confirmed anatomical reduction at articular sur-face and growth plate.All patients had been immobilized with short -leg cast for 4 -6 weeks.The ankle joint function and growth were evaluated by means of American Orthopaedic Foot and Ankle Society(AOFAS)Ankle Hindfoot Scale. Results All patients were followed up for 5 to 91 months with an average duration of 35 months.X -ray films showed that all fractures were bone healing.No uneven articular surface was found and there was no extremity rotation and shorte-ning deformity.Results were evaluated by AOFAS scoring system:excellent in 27 cases,good in 10 cases,and general in 2 cases.All patients were able to participate in the normal physical activities.Conclusions CT with multiplanar recon-struction is a premise to make an accurate diagnosis and to choose a reasonable approach.For failed closed reduction for adolescent distal tibial epiphyseal fractures,the cannulated screws are simple and may have a satisfactory effect.

OBJECTIVE To discuss the quality management of the recycled medical instrument.METHODS We executed the routine of recycled instrument seriously during our daily work,and gave strict measures during quality management such as callback,washing,classification,packaging,sterilization,handout,and inspection.RESULTS The recycled medical instrument had been used and managed for more than one year and the clinical work was ensured in progress smoothly and achieved satisfied results.CONCLUSIONS Actualizing the measure of quality control,perfecting the method of management and improving quality control could assure the safe and efficient use of recycled medical instrument and reach the standard of controlling hospital infection,and advancing medical nursing level.

Objective To evaluate the potential effects of RS504393, CC chemokine receptor (CCR) 2b and CCR1 antagonist, on LPS-induced acute lung injury (ALI) and to investigate the underlying mechanisms. Method A549 cell line was stimulated with LPS (10 μg/mL) and then treated with RS504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor (PAI)-l,monocyte chemoattractant protein (MCP)-2,and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry. Results In A549 cell line treated with RS504393,the expressions of CCR1, CCR2b and IL-8 were significantly inhibited after LPS stimulation. In rats with LPS-induced ALI, treatment with RS504393 significantly protected mice against lung injury by attenuating influx of leukocytes and protein into bronchoalveolar space and by lessening pathological changes of lung. Treatment with RS504393 down-regulated IL-1β and PAI-1 expressions in bronchoal veolar lavage fluid (BALF) and lungs at mRNA and protein levels along with up-regulation MCP-2 expression compared to rats of vehicle-treated groups. Conclusions CCR2b and CCR1 play pivotal roles in the development of ALl,and RS504393 as a antagonist can halt the development of ALI.

Object To determine and compare the content of polysaccharides in three species of Semen Plantaginis from different places of origin. Methods Spectrophotometry was used. Results The content of polysaccharides in the seed of Plantago major L. was higher than those in the seed of P. asiatica L. and P. depressa Willd. Conclusion The content of polysaccharides in Semen Plantaginis of different species or origins are obviously different.