Objective To observe the efficacy of the combination of moxibustion and pressing beans on auricular in the treatment of elderly patients with chronic diarrhea.Methods 45 elderly patients met the diagnostic criteria for diarrhea were divided into two groups,the treatment group (23 cases) received the treatment of combined moxibustion with ear pressure beans,the control group (22 cases) received traditional Chinese medicine treatment.The efficacy was observed and compared after two weeks.Results The effective rate reached 95.6％ in the treatment group,81.8％ in the control group (statistically significant difference,odds ratio 1.16).Conclusion Combined ear pressure beans with moxibustion treatment will receive a significant effect for the elderly patients with chronic diarrhea.The therapy is worth clinical application.

Objective: To observe influence of aerobic exercise on blood pressure in patients with white coat hypertension (WCH). Methods: A total of 51 newly diagnostic WCH patients were randomly divided into exercise group (n=26) and WCH control group (n=25). Patients in exercise group received aerobic exercise training (walking on flat ground by constant speed, twice a day, 50~80 min each time for three months); The WCH control group did not exercise. Office blood pressure (OBP) and 24h ambulatory blood pressure monitoring (ABPM) were measured in both groups before and three months after treatment. Results: There were no significant difference in indexes of OBP and ABPM between two groups before treatment (P>0.05). After three months, compared with before treatment, indexes of OBP and ABPM significantly decreased in exercise group and they were significantly lower than those of WCH control group in OBP [OSBP: (132.54±17.60) mmHg vs. (143.68±20.35) mmHg, ODBP: (89.12±18.39) mmHg vs. (92.75±17.24) mmHg] and ABPM [ mSBP：(121.57±20.86) mmHg vs. (128.06±19.45) mmHg, mDBP：(76.24±15.28) mmHg vs. (84.55±17.31) mmHg; dSBP：(124.27±22.50) mmHg vs. (130.85±24.61) mmHg, dDBP：(77.95±18.42) mmHg vs. (82.36±17.59) mmHg], P<0.05~0.01. Conclusions: Aerobic exercise can significantly improve blood pressure in patients with white coat hypertension.

Humans ; Medicine, Chinese Traditional ; Respiratory Tract Diseases ; prevention & control ; therapy ; virology ; Virus Diseases ; prevention & control ; therapy

Anti-Inflammatory Agents ; therapeutic use ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Ear Cartilage ; Female ; Humans ; Integrative Medicine ; methods ; Middle Aged ; Panniculitis ; complications ; drug therapy ; Phytotherapy ; Polychondritis, Relapsing ; complications ; drug therapy ; Prednisone ; therapeutic use ; Treatment Outcome

Camptothecin is known to possess a broad spectrum of anticancer activities, however due to its high toxicity and low solubility in water, camptothecin is severely limited in clinic. To optimize the effects of camptothecin in the treatment of malignancies, besides the structural modification and reconstruction of camptothecin, the establishment of new drug delivery systems becomes a hot topic in the camptothecin research. This paper aims to summarize the advanced progress in the delivery system and function of camptothecin at recent years. The advances in improving the poor solubility, lactone ring instability, controlled release systems and targeting delivery systems of camptothecin are also reviewed. As the related methods and technologies are improved and developed, camptothecin will be a group of prospective anticancer drugs.

Considering a variety of theories such like Dunkin's, Centra's and Kolitch's, a set of 13 indicators are designed to evaluate effect of courses taken in high education of China currently, compro-mised by chief aspects of course designing, practicing, and effect. Three courses including medical nursing, humanistic cultivation and communication skills in nursing and fundamentals of nursing in a nursing de-partment are chosen to be objects of questionnaire survey about the above indicators. The results show that there are significant differences in curriculum effectiveness between different courses. The scores are 2.81, 2.80 and 2.60 respectively, and teachers and students have different evaluation on the same indicators of the same curriculum,which provides a reference for teachers, colleges and universities and teaching management departments to promote the improvement of teaching quality.

Objective To evaluate the feasibility of catheter ablation for atrial fibrillation (AF) guided by 3 dimensional (3D) mapping combined with pulmonary vein (PV) circumferential mapping Methods 14 consecutive patients (M/F=10/4, age 52 4?12 8 y o, left atrium diameter 46 7?5 4 mm) with drug refractory paroxysmal ( n =10), permanent ( n =3) and persistent ( n =1) AF were included in this study Left atrial modification guided by 3D mapping system were performed first,and then, all PVs were isolated by circumferential mapping guided segmental ablation The endpoint of ablation included: (1) all left artial ablation lines finished; (2) all PVs were isolated and (3) non inducibility of AF was observed Results (1) Ten (71 4%) patients with paroxysmal AF reached the endpoint of the ablation completely (2) Total procedure and fluoroscopy time periods were 292?49 min and 54?9 min, respectively (3) After a mean follow up of 5 2?5 7(1-23)weeks, 7 (50%) patients with paroxysmal AF were free of AF and 3 (21 4%) patients with paroxysmal AF had significant improvement (4) No complications occurred during the procedure and the follow up period Conclusion Catheter ablation for AF guided by 3D mapping combined with PV circumferential mapping is feasible, safe and effective for patients with paroxysmal AF and left atrial enlargement

A set of specific primers was synthesized according to DNA sequence of HBV found in China, the reverse transcriptase (RT) region in polymerase gene was amplified by PCR method from the serum of 3 patients with chronic HBV infection, and then the PCR products were subcloned into pGEM Teasy vectors. 13 clones were sequenced. Sequence comparison of the selected clones was made to look for the difference. After being compared, 13 sequences of RT were found different. Besides 2 clones with long sequence deletion, the different rate of RT coding nucleic acid sequences, RT and HBsAg amino acid sequences of the 11 clones is 5 1%, 4 9% and 7 5%, respectively. Many mutation types, including point substitution and deletion mutation, were found in this region. There is quasispecies population and defective HBV genome in patients with chronic HBV infection.

T7 cDNA phage display method was employed to find the binding protein of PreSl protein. PreSl protein was coated in a 96-well ELISA plate, and then T7 cDNA library phages were bound to the target protein. Phages which did not bind to garget protein were washed away and the binding phages were eluted. Insertions from different clones were sequenced, and the deduced amino acid sequences were analyzed by Vector 6.0 software. Using BLAST software in GenBank, whole length of amino acid sequence of binding protein was obtained. After 4 rounds of biopanning, recombinant T7 phages with binding ablity were amplifed by infection to E. coli. One piece of amino acid sequence was found to be amino terminal of product of glioma tumor suppressor candidate region gene 2 (GLTSCR2). There was a binding domain KxPxKSGxxxL in these clones. T7 cDNA phage display technique can be used bo find the ligand. GLTSCR2 coding protein may be the binding protein to preSl protein of HBV.\;

Objective To construct plasmid pVR1012 M as nuclei acid vaccine for hepatitis B,was constructed to immunize mice with or without plasmid pcDNA 3.1 - IL 18 to identify the effect of Interleukin 18(IL 18). Methods Polymerase chain reaction method was used to amplify the PreS2 and S region of HBV and reconstruct plasmid pVR1012 M as nuclei acid vaccine. Plasmid pcDNA 3.1 - IL 18 was used as a co stimulator. Twenty five Balb/c mice were divided into 3 groups, group 1 immunized with 100 ?g plasmid pVR1012 group 2 pVR1012 M,Group 3,pVR1012 M with pcDNA 3.1 - IL 18, respectively, every 2 weeks for 3 times. Anti HBs were detected in serum 2 weeks after each injection. Lactated ehydrogenase (LDH) cytotoxicity assay was done to analyze the cytotoxic T lymphocytes funciton. Results The positive rate and the antibody titer of serum from mice injected pVR1012 M increasing gradually with the increasing frequency of inoculation, while those from mice injected pVR1012 M and pcDNA 3.1 - IL 18(joint group) were lower than those injected with pVR1012 M alone (Difference after 3rd inoculation was significant, P