Objective: To observe influence of aerobic exercise on blood pressure in patients with white coat hypertension (WCH). Methods: A total of 51 newly diagnostic WCH patients were randomly divided into exercise group (n=26) and WCH control group (n=25). Patients in exercise group received aerobic exercise training (walking on flat ground by constant speed, twice a day, 50~80 min each time for three months); The WCH control group did not exercise. Office blood pressure (OBP) and 24h ambulatory blood pressure monitoring (ABPM) were measured in both groups before and three months after treatment. Results: There were no significant difference in indexes of OBP and ABPM between two groups before treatment (P>0.05). After three months, compared with before treatment, indexes of OBP and ABPM significantly decreased in exercise group and they were significantly lower than those of WCH control group in OBP [OSBP: (132.54±17.60) mmHg vs. (143.68±20.35) mmHg, ODBP: (89.12±18.39) mmHg vs. (92.75±17.24) mmHg] and ABPM [ mSBP：(121.57±20.86) mmHg vs. (128.06±19.45) mmHg, mDBP：(76.24±15.28) mmHg vs. (84.55±17.31) mmHg; dSBP：(124.27±22.50) mmHg vs. (130.85±24.61) mmHg, dDBP：(77.95±18.42) mmHg vs. (82.36±17.59) mmHg], P<0.05~0.01. Conclusions: Aerobic exercise can significantly improve blood pressure in patients with white coat hypertension.

Objective To observe the efficacy of the combination of moxibustion and pressing beans on auricular in the treatment of elderly patients with chronic diarrhea.Methods 45 elderly patients met the diagnostic criteria for diarrhea were divided into two groups,the treatment group (23 cases) received the treatment of combined moxibustion with ear pressure beans,the control group (22 cases) received traditional Chinese medicine treatment.The efficacy was observed and compared after two weeks.Results The effective rate reached 95.6％ in the treatment group,81.8％ in the control group (statistically significant difference,odds ratio 1.16).Conclusion Combined ear pressure beans with moxibustion treatment will receive a significant effect for the elderly patients with chronic diarrhea.The therapy is worth clinical application.

Humans ; Medicine, Chinese Traditional ; Respiratory Tract Diseases ; prevention & control ; therapy ; virology ; Virus Diseases ; prevention & control ; therapy

Objective To observe the effect of iodine deficiency and hypothyroidism on the protein expressions of CREB in the entorhinal cortex of pups.Methods Twenty-eight female Wistar clean rats after pregnancy were randomly divided into control group,hypothyroid 1,2 group and iodine deficient group(7 in each group).From GD6 till PN28,iodine-deficient group was administered with iodine-deficient diet [iodine content:(14.11 ?1.96) ng/g] and tap water;hypothyroid 1 and 2 group were administered with 5 mg/L and 15 mg/L PTU in the drinking water and fed with normal diet [iodine content:(470.50?46.52) ng/g].Control group received tap water and normal diet during the experiment.Five pups from each group were sacrificed and intracardiac perfused at PN7,PN14,PN21,PN28 and PN42.Brains were removed,fixed and sectioned coronally.All sections were observed and were analyzed for the protein expression of CREB by immunohistochemistry in the entorhinal cortex.Results At PN7,there was no significant difference in the expression of Ng in all four groups.At PN14 and PN21,the expression of CREB in the entorhinal cortex in hypothyroid 1,2 group and iodine deficient group were significantly lower than those of controls(P

A set of specific primers was synthesized according to DNA sequence of HBV found in China, the reverse transcriptase (RT) region in polymerase gene was amplified by PCR method from the serum of 3 patients with chronic HBV infection, and then the PCR products were subcloned into pGEM Teasy vectors. 13 clones were sequenced. Sequence comparison of the selected clones was made to look for the difference. After being compared, 13 sequences of RT were found different. Besides 2 clones with long sequence deletion, the different rate of RT coding nucleic acid sequences, RT and HBsAg amino acid sequences of the 11 clones is 5 1%, 4 9% and 7 5%, respectively. Many mutation types, including point substitution and deletion mutation, were found in this region. There is quasispecies population and defective HBV genome in patients with chronic HBV infection.

T7 cDNA phage display method was employed to find the binding protein of PreSl protein. PreSl protein was coated in a 96-well ELISA plate, and then T7 cDNA library phages were bound to the target protein. Phages which did not bind to garget protein were washed away and the binding phages were eluted. Insertions from different clones were sequenced, and the deduced amino acid sequences were analyzed by Vector 6.0 software. Using BLAST software in GenBank, whole length of amino acid sequence of binding protein was obtained. After 4 rounds of biopanning, recombinant T7 phages with binding ablity were amplifed by infection to E. coli. One piece of amino acid sequence was found to be amino terminal of product of glioma tumor suppressor candidate region gene 2 (GLTSCR2). There was a binding domain KxPxKSGxxxL in these clones. T7 cDNA phage display technique can be used bo find the ligand. GLTSCR2 coding protein may be the binding protein to preSl protein of HBV.\;

Objective To construct plasmid pVR1012 M as nuclei acid vaccine for hepatitis B,was constructed to immunize mice with or without plasmid pcDNA 3.1 - IL 18 to identify the effect of Interleukin 18(IL 18). Methods Polymerase chain reaction method was used to amplify the PreS2 and S region of HBV and reconstruct plasmid pVR1012 M as nuclei acid vaccine. Plasmid pcDNA 3.1 - IL 18 was used as a co stimulator. Twenty five Balb/c mice were divided into 3 groups, group 1 immunized with 100 ?g plasmid pVR1012 group 2 pVR1012 M,Group 3,pVR1012 M with pcDNA 3.1 - IL 18, respectively, every 2 weeks for 3 times. Anti HBs were detected in serum 2 weeks after each injection. Lactated ehydrogenase (LDH) cytotoxicity assay was done to analyze the cytotoxic T lymphocytes funciton. Results The positive rate and the antibody titer of serum from mice injected pVR1012 M increasing gradually with the increasing frequency of inoculation, while those from mice injected pVR1012 M and pcDNA 3.1 - IL 18(joint group) were lower than those injected with pVR1012 M alone (Difference after 3rd inoculation was significant, P

Objective T7 cDNA phage display system and bioinformatics methods were employed to find the binding protein to the PreS1 protein of hepatitis B virus (HBV). Methods PreS1 protein was coated in ELISA plate as the target protein, and then T7 cDNA library phage display system was used to scan the binding protein or peptide. A piece of cDNA was found to have the function to bind the PreS1 protein, and the product was named as PreS1 binding protein (PreS1BP). Using BLAST in GenBank, the amino acid sequence of PreS1BP was compared in the protein sequence database. Results The amino acid sequence of PreS1BP was identified as a piece of glioma tumor suppressor candidate region gene 2 (GLTSCR2), and the length of cDNA of PreS1BP was proved to be 1436 nt. The gene was located at chromosome 19q arm (19q13.3) with a length of 11445 base pair between 10403483 and 10414989, containing 13 exons and 12 introns. Conclusion HBV PreS1BP gene could be obtained by T7 cDNA phage display system in combination with bioinformatics methods.

Objective:To study the expression and clinical significance of GPRC5A and SOCS3 in colorectal carcinoma.Methods:SP immunochemical method was performed to detect the expression of GPRCSA and SOCS3 in 45 cases of colorectal carcinoma,25 cases of colorectal adenomas and 22 cases of normal colorectal tissues.Results:1)Expression of GPRC5A in colorectal cancinoma tissue (22.2％) was significantly lower than that in adenomas tissue (52.0％,P＞0.05).The Latter was significantly lower than that in normal colorectal tissue (81.8％,P＜0.05).GPRC5Awas closely related to lymph node metastasis,Duke's stages and the deepness of invasion (P＜0.05).2) Expression of SOCS3 in colorectal cancinoma tissue (24.4％) was significantly lower than that in adenomas tissue (56.0％,P＜0.01).The Latter was significantly lower than that in normal colorectal tissue (86.4％,P＜0.05).SOCS3 was closely related to pathological differentiation,the deepness of invasion,Duke's stages and lymph node metastasis (P＜0.05).3)The expression of GPRC5A was positive correlated with SOCS3 (P＜0.05).Conclusions:The reduced expressions of GPRC5A and SOCS3 may participate in the occurence and development of colorectal carcinorma,suggesting that GPRC5A and SOCS3 may act as biological markers for evaluating the malign degree,prognosis and therapeutic targets of colorectal carcinorma.

Objective To evaluate the feasibility of catheter ablation for atrial fibrillation (AF) guided by 3 dimensional (3D) mapping combined with pulmonary vein (PV) circumferential mapping Methods 14 consecutive patients (M/F=10/4, age 52 4?12 8 y o, left atrium diameter 46 7?5 4 mm) with drug refractory paroxysmal ( n =10), permanent ( n =3) and persistent ( n =1) AF were included in this study Left atrial modification guided by 3D mapping system were performed first,and then, all PVs were isolated by circumferential mapping guided segmental ablation The endpoint of ablation included: (1) all left artial ablation lines finished; (2) all PVs were isolated and (3) non inducibility of AF was observed Results (1) Ten (71 4%) patients with paroxysmal AF reached the endpoint of the ablation completely (2) Total procedure and fluoroscopy time periods were 292?49 min and 54?9 min, respectively (3) After a mean follow up of 5 2?5 7(1-23)weeks, 7 (50%) patients with paroxysmal AF were free of AF and 3 (21 4%) patients with paroxysmal AF had significant improvement (4) No complications occurred during the procedure and the follow up period Conclusion Catheter ablation for AF guided by 3D mapping combined with PV circumferential mapping is feasible, safe and effective for patients with paroxysmal AF and left atrial enlargement