1.Determination of camphor, menthol, synthetic borneol and methyl salicylate in four kinds of TCM adhesive plasters by GC
Jie LIU ; Wenling DONG ;
Chinese Traditional Patent Medicine 1992;0(10):-
AIM: To establish the determination for camphor, menthol, synthetic borneol, and methyl salicylate in four kinds of TCM adhesive plasters by GC. METHODS: The internal standard method was employed. Naphthalene, the internal standard, was added to the sample before treatment. The sample solution was prepared by distillation with essential oil collector. The GC system consisted of glass column, 10% PEG 20M as the stationary phase, nitrogen as the carrier gas, column temperature at 130℃, and FID as the detector. RESULTS: Camphor, menthol, synthetic borneol(borneol and isoborneol), and methyl salicylate in 6 lots of samples and naphthalene have been separated well under the chromatographic condition. In the methodology study on Fengshi Zhitong Adhesive Plasters, the average recovery of camphor, menthol, synthetic borneol, and methyl salicylate were 95.54%( RSD =1.6%), 96.90%( RSD =2.2%), 97.30%( RSD =1.4%), and 96.48%( RSD =2.8%), respectively. CONCLUSION: The method is sensitive, accurate and reproducible, and can be used to control the quality of the four kinds of preparations.
2.Pathology of cerebral small vessel disease
Jie WANG ; Xinyi LIU ; Hairong DONG
International Journal of Cerebrovascular Diseases 2015;(9):704-709
Cerebral smal vessel disease (CSVD) accounts for 25 to 30% of al strokes. It is a main cause of vascular cognitive impairment. Because of cerebral smal vessels can not easily be directly observed and studied in vivo and on imaging, so the term CSVD is generaly used to describe a syndrome of clinical, cognitive, neuroimaging, and neuropathological changes, and not focus on the pathological changes of the smal vessel wal itself that caused these syndromes. This article reviews the advances in research on CSVD, particularly its pathological changes of vessel wals.
3.Effect of propofol anesthesia on electroconvulsive therapy-induced hyperphosphorylation of Tau protein in hippocampus in depressed rats
Chao LIU ; Su MIN ; Ke WEI ; Dong LIU ; Jun DONG ; Jie LUO ; Ping LI ; Xiaobin LIU
Chinese Journal of Anesthesiology 2012;32(3):295-297
Objective To investigate the effect of propofol anesthesia on electroconvulsive therapy (ECT)-induced hyperphosphorylation of Tau protein in hippocampus in depressed rats.Methods Thirty-two female WYK rats in which the total score was 30-120 after Open-field test,aged 24 weeks,weighing 200-250 g,were randomly divided into 4 groups ( n =8 each):control group (group C),propofol group (group P),ECT group (group E)and propofol + ECT group (group PE).In groups C and E,the animals received intraperitoneal normal saline 5 ml,and in addition the animals received ECT 15 min later in group E.In groups P and PE,the animals received intraperitoneal 100 mg/kg propofol 5 ml,and in addition the animals received ECT 15 min later in group PE.The learning and memory function was assessed by Morris water maze test at 24 h after ECT.The animals were sacririced at 6 h after Morris water maze test and the hippocampal tissues were removed for determination of the expression of phosphorylated Tau protein.Results Compared with group C,the escape latency was significantly prolonged,the swimming time was significantly shortened in groups P,E and PE,the expression of phosphorylated Tau protein in hippocampus was down-regulated in group P,and the expression of phosphorylated Tau protein in hippocampus was up-regulated in group E ( P < 0.05).Compared with group E,the escape latency was significantly shortened,the swimming time was significantly prolonged,and the expression of phosphorylated Tau protein in hippocampus was down-regulated in group PE (P <0.05).Conclusion The mechanism by which propofol anesthesia improves cognitive impairment induced by ECT may be related to inhibition of hyperphosphorylation of Tau protein in hippocampus in depressed rats.
5.Anti-infection action of Bazheng mixture(BM) and effects of BM on immune function in mice
Ruyi LIU ; Huixun REN ; Jie WU ; Yingying DONG
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(01):-
Objective To explore the pharmacological action of Bazheng mixture(BM) on infections disease of urinary system and effects of BM on immune function in mice.Methods After E.coli was injected into bladder of mice, the kidneys were examined for the anti-infection action of BM on ascending infection;and the effects of BM on immune function of mice were tested.Results BM ig remarkably decreased the percentage of kedney's infections area caused by ascending infection of E.coli [ED_(50) was(11.01?1.63)g/kg and the 95% CL was (9.50-12.76)(g/kg]);BM was also found effective in increasing the rate of carbon particle clearance and the index of carbon particle clearance,but BM had no significant effects on body fluid immunity and cell immunity in test for mice.Conclusion The mechanism of BM in treating infections disease of urinary system are mainly associated with the increasing rate of carbon particle clearance,and with bacteria clearance in urinary system.
6.CT, MRI and PET-CT in the diagnosis of cervical cancer
Dong YANG ; Hongwen ZHANG ; Hui LIU ; Jie QI
Journal of International Oncology 2014;41(3):220-223
With the increase of the incidence and the refinement of clinical treatment,the clear preoperative staging and the assessment of tumor recurrence and metastasis of cervical cancer is particular important.Different results may be explained in the same tumor applied different methods of imaging examination,which influences the clinical diagnosis and treatment.So imaging examination plays a more and more important role in the assessment of preoperative staging and tumor recurrence.
7.Effects of survivin inhibitor YM155 on mitochondrial apoptotic pathway of retinoblastoma Y79 cells
Dong WEI ; Xiaojuan ZHANG ; Jie LIU ; Qiuying SONG
Chinese Journal of Pathophysiology 2017;33(1):26-32
AIM:To investigate the effects of survivin inhibitor YM155 {4,9-dihydro-1-(2-methoxyethyl)-2-methyl-4,9-dioxo-3-(2-pyrazinylmethyl)-4,9-dihydro-1H-naphtho[2,3-d]imidazolium bromide} on the apoptosis, mito-chondrial membrane potential (Δψm) and cytochrome C (Cyt C) of retinoblastoma Y79 cells, and to analyze the mitochon-drial mechanisms of apoptosis .METHODS:Y79 cells were cultured in vitro and treated with YM155 at concentrations of 0, 0.5, 1, 2, 4 and 8 nmol/L.The cells in control group were treated without YM 155.The proliferation of Y79 cells were measured by CCK-8 assay and bromodeoxyuridine ( BrdU) labeling assay .Y79 cells were randomly divided into 4 groups:control group ( with equal volume of RPMI-1640 nutrient medium ) , positive control group ( 10 nmol/L topotecan ) , low-dose (1 nmol/L) YM155 group and high-dose (2 nmol/L) YM155 group.The effects of YM155 on the apoptosis, the changes of Δψm , the mitochondrial distribution and the protein level of Cyt C in the Y 79 cells were evaluated by flow cytom-etry with Annexin V-FITC/PI staining, JC-1 staining, immunofluorescence analysis and Western blot , respectively.RE-SULTS:Compared with control group , YM155 significantly inhibited the proliferation of Y 79 cells and induced apoptosis (P<0.05).YM155 significantly reduced Δψm of the Y79 cells, promoted Cyt C which released from mitochondria to the cytosol and reduced the protein level of Cyt C in the mitochondria (P<0.05).CONCLUSION:YM155 inhibits Y79 cell proliferation and induces apoptosis , and the possible mechanisms may be involved in the mitochondrium-mediated apoptotic pathway .
8.Organ dose modulation in chest CT scan in female: protection of breasts
Mingyue WANG ; Junqiang DONG ; Jianbo GAO ; Jie LIU ; Ping HOU
Chinese Journal of Radiological Medicine and Protection 2016;36(7):530-533
Objective To study the effects of organ dose modulation (ODM) technique on dose reduction of the breasts and the related thoracic image quality in female chest CT.Methods One hundred and twelve female patients with chest CT were enrolled in this study and divided into two groups according to the order:control group (n =56,using conventional scan) and experimental group (n =56,using ODM technique).The tube currents in different directions (A/L/P/R) were analyzed in the two groups.The effects of ODM on the radiation dosage and image quality were assessed.Results The tubc currents in anterior and posterior direction were both (128 ± 43)mA in the control group.However,the tube current in the anterior was lower than that in the posterior in experimental group (t =-18.701,P <0.01).The tube currents in all direction in the experimental group were all lower than those in the control group (t =11.71-20.22,P <0.01).The CTDIvol and E in the experimental group were lower than those in the control group(t=3.58,3.55,P <0.05).There were no significant differences for the objective and the subjective scores between two groups (P > 0.05).Conclusions ODM technique could protect the female breasts by reducing the radiation dose without image quality degrading during chest CT scan.
9.Effect of ischemic time on survival after heart transplantation
Yongfeng SUN ; Nianguo DONG ; Jinping LIU ; Jie CAI ;
Chinese Journal of Organ Transplantation 2014;35(6):329-332
Objective Given the significant differences of ischemia-time tolerance observed in clinical heart transplantation between heart and other solid organs,it is important to make a clinical assessment of the correlation between the cold ischemic time of the donor heart and the survival rate after heart transplantation.With these results,we may standardize the management of clinical heart transplantation by providing a proper heart cold ischemic time frame for reference.Method The clinical data of 131 orthotopic heart transplantation patients in our hospital,from September 2008 to March 2014,were collected and analyzed retrospectively.All donor hearts were preserved in histidinetryptophan-ketoglutarate solution (HTK solution) during cold ischemic time.The cold ischemia time was 103-485 min,with an average of 245.2 ± 120.4 min.According to the cold ischemic time,all patients were divided into three groups:< 3 h (n =62); 3-6 h (n =41); >6 h (n =28).Result (1) Prolonged cold ischemia time could result in high IABP usage perioperatively (postoperative IABP support rate for the three groups was 3.2%,9.8% and 14.3% respectively,P =0.155) and high rejection rate (incidence of rejection was 6.4%,9.8% and 17.9% respectively,P =0.245),but there was no statistically significant difference.(2) Three weeks after the transplantation,all EF values of the three groups were reduced within the normal range,with no significant difference.Perioperative overall survival rate was 97.7% (128/131),while survival rate of the three groups was 97.29% (72/74),100% (30/30) and 96.29% (26/27),respectively (P =0.61).(3) One-year overall survival rate was 89.87% (71/79),and the one-year survival rate of three groups was 92.2% (47/51),90.9% (10/11) and 82.4% (14/17) respectively (P=0.51).Fifty-two patients were still under 1 year follow-up period.This study aimed to illustrate the effect of different cold ischemic time on perioperative cardiac function,rejection rate,IABP usage postoperatively (intra-aortic balloon pump or intra-aortic balloon counterppulsation) and early/mid-term efficacy after transplantation.Conclusion Cold ischemic time within 6-8 h is clinically safe for heart transplantation,and can provide satisfactory early and medium-term effect.Donor heart with cold ischemia time longer than 6 h may extend the recipient inclusion criteria.But considering the safety of transplantation,these donor hearts may be more applicable for the marginal recipients.This study describes the relationship between cold ischemic time and early and medium-term effect of heart transplantation.However,its long-term effects still require further investigation.
10.Research progress of detecting large-diameter tail in lipid injectable emulsions.
Jie PENG ; Wujun DONG ; Lin LI ; Xuejun XIA ; Yuling LIU
Acta Pharmaceutica Sinica 2014;49(7):956-62
Injectable lipid emulsions have been routinely used in patients since 1960s as a nutritional supplement for patients requiring parenteral nutrition. In recent years, lipid injectable emulsions have been extensively studied as a kind of novel drug carrier, also the quality problems of the lipid emulsion attract more and more attentions gradually. Large diameter tail of injectable lipid emulsions as a significant quality control indicator should pay more attention. Regarding to the defect of detecting large diameter tail of lipid injectable emulsions in our country, the purpose of this article is to summarize the techniques of detecting large diameter tail, illustrate the impacts of large lipid droplet on the quality of lipid injectable emulsions, emphasize the importance of detecting large diameter tail in lipid emulsions and provide guidance for researching and developing lipid emulsions in domestic market.