2.Analysis of the causes of postoperative hemorrhage of tracheotomy in patients with liver transplantation.
Bao-dong WANG ; Ai-hua SUN ; Bin-hua DENG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2006;41(12):953-954
Adult
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Aged
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Female
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Humans
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Liver Transplantation
;
adverse effects
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Male
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Middle Aged
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Postoperative Hemorrhage
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etiology
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Tracheotomy
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adverse effects
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Young Adult
4.Influence of hypoxia on cell proliferation and expression of p21 in rhesus retinal vascular endothelial cells
Hua-bin, ZHENG ; Jin-dong, HAN ; Zhi-gang, YUAN ; Hua, YAN
Chinese Journal of Experimental Ophthalmology 2012;(11):972-975
Background Retinal neovascularization disease is a common cause of blinding.Retinal neovascularization is related to enhancing proliferation of vascular endothelial cells.So how to inhibit proliferation of vascular endothelial cells is a hot burning issue.p21 is known to be involved in the regulation of cell cycle and therefore inhibit the cell proliferation.However,the relationship of p21 and the proliferation of vascular endothelial cells in retinal neovascularization disease is for further study.Objective The aim of this experiment was to study the proliferation of rhesus retinal vascular endothelial cells(RF/6A) and expression of p21 in RF/6A cells under the hypoxia condition,and discuss their association.Methods The RF/6A cells were cultured and passaged in vitro,then they were randomly divided into normoxia culture group(5% CO2 +95% O2) and hypoxia for 1 hour,3,6,12 hours group(1% 02+5% CO2 +94% N2).Flow cytometer(FCM) was used to check the distribution of RF/6A cell cycle in the normoxia culture group and hypoxia for 1 hour,3,6,12 hours groups.MTT assay was used to detect and compare the cell proliferation(A570)among the various groups.The expression of p21 in the cells was analyzed by Western blot.Results FCM showed that the cells proportion of G0/G1 stage was reduced initially and then increased afterward in hypoxia for 1 hour and 3,6,12 hours groups,showing a significant difference among 5 groups (F =20.083,P =0.000),and the cells proportion of S stage and G2/M stage were increased firstly and then declined in different hypoxia groups with statistical significances (F =7.861,P =0.001 ; F =10.305,P =0.003).Compared with normoxia culture group,cells proportion of G0/G1 stage was declined and that of S stage and G2/M stage were raised after hypoxia culture,showing statistically signifcant differences(P<0.05).MTT showed that cell multiplication capacity(A570 value)strengthened firstly and then weakened in hypoxia groups with time prolongation,showing a significant difference among all the groups(F=7.768,P=0.001),and A570 value in hypoxia for 3 hours and 6 hours groups (0.315± 0.062,0.365 ± 0.064) was significantly higher than that of the normoxia group (0.205 ± 0.063),respectively(P<0.05).Western blot showed that the expression of p21 in the cells down-regulated at the beginning and then up-regulated with the increase of hypoxia time,and there was statistical significance (F =16.738,P=0.000).The p21 relative levels in different hypoxia groups were reduced in comparison with the normoxia group,showing statistical signifcances(P<0.05).Conclusions Short-term hypoxia could reduce the expression of the p21 in RF/6A and induce cell proliferation initially,then p21 increases and cell proliferation is inhibited with the prolongation of hypoxia time.
5.Progress on the Study of Sex-specific Membrane Proteins in Mammalian Spermatozoa
Dong WANG ; Zhen-Ling WANG ; Jin-Hua CHENG ; Hua-Bin ZHU ; Hai-Sheng HAO ; Yong-Hua LIU ;
China Biotechnology 2006;0(01):-
The expression of specific genes in sex chromosomes is the basis of sex-specific membrane protein in mammalian spermatozoa. The gene expression products are shared among spermatozoa through intercellular bridges, however, the phenomena of male transmission-ratio distortion and sex ratio distortion proved that differential proteins exist between X and Y spermatozoa. In addition, the existence of sex-specific proteins was confirmed by the separation experiment of X/Y chromosome bearing spermatozoa and the detection result of sex specific proteins. At the same time, it was also confirmed that the difference of the sex-specific protein is weak . The advance of separation techniques as well as the integration and optimization among these techniques has made it possible to separate sex-specific membrane proteins in mammalian spermatozoa.
6.Establishment of a scoring system for predicting the positive rate of prostatic biopsy for prostate cancer.
Jian-gang CHEN ; Xin-feng CHEN ; Dong-hua GU ; Ming LU ; Bing ZHENG ; Bing ZHANG ; Bin PAN ; Hua ZHU ; Xiao-dong PAN ; Bin XU ; Lin QIAN
National Journal of Andrology 2015;21(1):53-56
OBJECTIVETo identify the predictors of the positive results of transrectal ultrasound (TRUS)-guided biopsy for prostate cancer.
METHODSWe performed univariate and multivariate logistic regression analyses on the relevant data on 385 male patients that underwent TRUS-guided biopsy for prostate cancer, including such potential predictors as age, body mass index (BMI), symptoms, results of digital rectal examination (DRE), tPSA, fPSA, free/total PSA ratio (f/tPSA), prostate volume (PV), and PSA density (PSAD) for identification of the risk factors related to the positive rate of biopsy. Then we constructed a scoring system as a tool for predicting prostate cancer in repeat biopsies and determined the sensitivity of the system by calculating the false positive rate using the receiver operating characteristic curve.
RESULTSAmong the 385 patients, 139 (36.1%) were diagnosed with prostate cancer. On multivariate analysis, age (P < 0.01), DRE (P < 0.01), tPSA (P < 0.01), fPSA (P < 0.01), f/tPSA (P < 0.01), PV (P < 0.01), and PSAD (P < 0.01) were all significant predictors of prostate cancer. Multivariate logistic regression analysis showed age, tPSA, f/tPSA, PV, and PSAD to be independent predictors, with ORs and 95% CIs of 1.07 (1.05-1.16), 1.05 (1.02-1.15), 0.97 (0.86-0.99), 0.98 (0.87-0.96), and 1.79 (1.48-2.06), respectively. Moreover, patients with the risk score of 3-5 had a significantly higher rate of prostate cancer than those with 0-2 (64% vs 11%, P < 0.001).
CONCLUSIONThe scoring system on the key predictors of prostate cancer can help urologists to identify the men in need of prostatic biopsy.
Aged ; Digital Rectal Examination ; Humans ; Image-Guided Biopsy ; methods ; Logistic Models ; Male ; Middle Aged ; Prostate ; pathology ; Prostate-Specific Antigen ; analysis ; Prostatic Neoplasms ; chemistry ; pathology ; ROC Curve ; Risk Factors ; Ultrasonography, Interventional ; methods
7.Study on the Yersinia pestis plasmid along the Qinghai-Tibet Railway
Chun-hua, ZHANG ; Jing-sheng, L(U) ; Bin, ZHAO ; Xian-bin, CONG ; Zhong-hui, WANG ; Shi, ZHANG ; Kui-dong, SHAO
Chinese Journal of Endemiology 2008;27(6):595-597
Objective To detect the Yersinia pestis plasmid and molecular weight along the Qinghai-Tibet Railway.Methods Yersinia pestis plasmids molecular weight detected and analyzed using alkaline lysis,phenol-chloroform extraction of Yersinia pestis plasmid by agarose gel electrophoresis.Results The 18 Yersinia pestis strains of Qinghai-Tibet Railway contained 6×106,45×106,52×106,65×106,92×106plasmid,varing in the range of the 52×106-92×106.Conclusions The Yersinia pestis of Qinghai-Tibet Railway has a standardplasmid graphics,with the biggest Yersinia pestis plasmid changing in a certain regular degree,which providessignificance in the study of plague natural foci of the spatial structure and the genetic.characteristics of Yersiniapestis.
8.Establishment of prescription research technology system in Chinese medicine secondary exploitation based on "component structure" theory.
Xu-Dong CHENG ; Liang FENG ; Jun-Fei GU ; Ming-Hua ZHANG ; Xiao-Bin JIA
China Journal of Chinese Materia Medica 2014;39(21):4272-4276
Chinese medicine prescriptions are the wisdom outcomes of traditional Chinese medicine (TCM) clinical treatment determinations which based on differentiation of symptoms and signs. Chinese medicine prescriptions are also the basis of secondary exploitation of TCM. The study on prescription helps to understand the material basis of its efficacy, pharmacological mechanism, which is an important guarantee for the modernization of traditional Chinese medicine. Currently, there is not yet dissertation n the method and technology system of basic research on the prescription of Chinese medicine. This paper focuses on how to build an effective system of prescription research technology. Based on "component structure" theory, a technology system contained four-step method that "prescription analysis, the material basis screening, the material basis of analysis and optimization and verify" was proposed. The technology system analyzes the material basis of the three levels such as Chinese medicine pieces, constituents and the compounds which could respect the overall efficacy of Chinese medicine. Ideas of prescription optimization, remodeling are introduced into the system. The technology system is the combination of the existing research and associates with new techniques and methods, which used for explore the research thought suitable for material basis research and prescription remodeling. The system provides a reference for the secondary development of traditional Chinese medicine, and industrial upgrading.
Drug Prescriptions
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Medicine, Chinese Traditional
9.An osteoclast-rich tumor of the gastrointestinal tract with features resembling clear cell sarcoma of soft parts: a case report and review of the literature.
Dong-Jie LI ; Xin-Hua ZHANG ; Wen-Bin HUANG ; Kui MENG ; Xiao-Jun ZHOU
Chinese Journal of Pathology 2005;34(11):757-758
Adult
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Antigens, Neoplasm
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metabolism
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Gastrointestinal Neoplasms
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metabolism
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pathology
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surgery
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Gastrointestinal Stromal Tumors
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pathology
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Humans
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MART-1 Antigen
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Male
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Neoplasm Proteins
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metabolism
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Osteoclasts
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pathology
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S100 Proteins
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metabolism
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Sarcoma, Clear Cell
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metabolism
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pathology
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surgery
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Soft Tissue Neoplasms
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metabolism
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pathology
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surgery
10.Establishment of industry promotion technology system in Chinese medicine secondary exploitation based on "component structure theory".
Xu-Dong CHENG ; Liang FENG ; Ming-Hua ZHANG ; Jun-Fei GU ; Xiao-Bin JIA
China Journal of Chinese Materia Medica 2014;39(20):4065-4069
The purpose of the secondary exploitation of Chinese medicine is to improve the quality of Chinese medicine products, enhance core competitiveness, for better use in clinical practice, and more effectively solve the patient suffering. Herbs, extraction, separation, refreshing, preparation and quality control are all involved in the industry promotion of Chinese medicine secondary exploitation of industrial production. The Chinese medicine quality improvement and industry promotion could be realized with the whole process of process optimization, quality control, overall processes improvement. Based on the "component structure theory", "multi-dimensional structure & process dynamic quality control system" and systematic and holistic character of Chinese medicine, impacts of whole process were discussed. Technology systems of Chinese medicine industry promotion was built to provide theoretical basis for improving the quality and efficacy of the secondary development of traditional Chinese medicine products.
Chemistry, Pharmaceutical
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economics
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standards
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China
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Drug and Narcotic Control
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economics
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Drugs, Chinese Herbal
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chemistry
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economics
;
standards
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Humans
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Medicine, Chinese Traditional
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economics
;
standards
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Quality Control