Objective To investigate the effect of pidotimod combined with antiviral drugs on immunologic function and myocardial enzyme spectrum in children with infectious mononucleosis.Methods 94 cases of children with infectious mononucleosis selected in Wenzhou Children's hospital were randomly divided into control group and research group,47 cases in each group.The control group were given conventional antiviral treatment; On this basis,the research group were given pidotimod,2 weeks for one period of treatment.Before and after treatment,immune function,cell factors and myocardial enzyme spectrum were tested,the clinical symptoms,signs,the curative effect and complications were observed and compared.Results Compared with before treatment,after treatment,serum CD4+,CD4+/CD8+ IgA and IgG increased,CD8+ decreased,TNF alpha,IL-6,AST,LDH,CK,CK-MB decreased(P<0.05).Compared with the control group,in the research group,CD4+,CD4+/CD8+,IgA and IgG content is higher,CD8+ content is lower,the TNF alpha,IL-6,AST,LDH,CK,CK-MB content is lower(P<0.05).The time of antipyretic,angina fade,narrow lymph nodes,liver and spleen shrinks and length of hospital stay in the research group were shorter than that in the control group(P<0.05).The effective rate was 74.47％in the control group,lower than 91.49％of research group(P<0.05).Conclusion The curative is exact that pidotimod combined with antiviral drugs on the treatment of infectious mononucleosis.It could improve immune function,reduce myocardial enzyme spectrum and inflammation.

Objective:To measure the physiological response and subjective experience differences between feminine and masculine women inducing anger,exploring women's susceptibility to depression.Methods:Thirty feminine women and 26 masculine women were selected from 745 college women students who filled in the Chinese College Students'Sex Role Inventory-50 items (CSRI-50),according to median classification method.Physiological response,including heart rate,skin conduct,respiration rate were recorded using Biotrace software from 5-10 min before watching films to films end,state anger were measured before and after films by the State Anger Scale(SAS),one of the State-Trait Anger Expression Inventory-Ⅱ (STAXI-Ⅱ).Results:With inducing anger,the skin conducts were lower in feminine women than in masculine women[(6.1 ±4.6) μmho vs.(9.4 ±6.0)μmho,P ＜0.05],while the respiration rates were higher in feminine women than in masculine women[(19.4 ±2.8) bpm vs.(17.7 ± 3.3) bpm,P ＜ 0.05].The SAS sores were higher in feminine women than in masculine women[(31.6 ± 8.8) vs.(26.5 ± 9.2),P ＜ 0.05].Conclusion:It suggests that feminine women subjectively report more anger and physiological responses may be greater in watching anger inducing films,mainly in RSP rate,however masculine women's SC is higher,which indicates that future researches of anger and depression should consider sex role.

Humans ; Nerve Growth Factor ; physiology ; Pain ; physiopathology ; Pulpitis ; physiopathology ; Receptor, Nerve Growth Factor ; physiology ; Receptor, trkA ; physiology

Objective To elucidate clinical and pathological features of obesity-associated focalsegmental glomerulosclerosis(FSGS). Methods Clinicopathological data of thirteen obese cases (BMI3≥28kg/m2) with FSGS(OB-FSGS) and thirteen non-obese cases(BMI

Carcinoma, Hepatocellular ; surgery ; Catheter Ablation ; Humans ; Liver Neoplasms ; surgery

Objective To study the activation effect of HBV RNase H protein on the transcription of cellular apoptosis susceptibility gene (CAS). Methods The promoter of DNA sequence of CAS gene was identified in GenBank by bioinformatics and amplified from HepG2 genome by PCR using sense (5′-GGTACCCGATTACATGTTGTACATGAAGG-3′) and antisence (5′-CTCGAGGCTGAGTTCCATTGCTATAG-3′) primers. As these primers contained Kpn I and Xho I recognition sites on their respective 5′-ends, the amplified DNA fragments were tested by sequencing and then subcloned into Kpn I/Xho I sites of pCAT3-Basic reporter vector by routine molecular biological methods. The reconstructed plasmid named pCAT3-CASp was identified by enzyme digestion of Kpn I/Xho I, in which the expression of chloramphenical acetyltransferase (CAT) was under the control of the promoter of CAS. The HepG2 cells were transfected by pCAT3-CASp, and then co-transfected by pCAT3-CASp and pcDNA3.1(-)-RH plasmids. At the same time, the empty pCAT3-basic and pCAT3-TXNRD1p were transfected (self-contructed by the authors) as controls. After 24h culturing, cells were collected and the expression of CAT activity was detected by ELISA according to the manufacturer′s protocol. Results The optical density of expression of CAT of pCAT3-CASp was 0.043 by ELISA, in contrast, the optical density of expression of pCAT3-Basic was 0.024. The expression of CAT in co-transfection of pCAT3-CASp and pcDNA3.1(-)-RH(0.065) was 1.5 times as higher as pCAT3-CASp plasmid (0.043), and 2.7 times as higher as pCAT3-Basic. Conclusions The CAS gene promoter identified in present study has transcription activity and HBV RNase H protein may activate the expression of CAS gene.

Poly-?-hydroxybutyric acid (PHB) produ c ing strain NS-82# which was isolated from the soil was identified to be Bacillus sp.The purified sample from fermentation was similar to t he standard sample produced by Aldric Chemical Company Inc after determinated wi th ultraviolet absorption,IR-absorption,gas chromatographiyc and nuclea r magnetic resonance analysis of polyesters.

Aged ; Aged, 80 and over ; Corynebacterium ; isolation & purification ; Cross Infection ; epidemiology ; microbiology ; Female ; Humans ; Male ; Random Amplified Polymorphic DNA Technique

Objective To discuss the impact on serum interleukin-4 and interleukin-17 in patients with hypertension and early renal damage treated with intermediate frequency If pulse electrical stimulation on acupuncture points. Methods Selected 100 patients diagnosed with early renal damage in hypertension, those were divided into control group and treatment group randomly,50 patients in the control group treated with conventional antihypertensive drugs and symptomatic treatment of diseases, while the treatment group on the basis of conventional therapy plus IF pulse point therapy,20 days after treatment compared serum IL-4 and IL-17 levels. Results Treatment group,serum IL-14 and IL-17 levels were significantly lower than the control group (all P ＜ 0.05). Conclusion Shenshu, Mingmen ,Guanyuan and other points by electrical stimulation pulse frequency ,could make IL-4 ,IL-17 and other indicators of early renal damage improve the role, and reduce hypertension early target organ damage, and help reduce pressure and reduce the amount of antihypertensive drugs.

Hyperthermia is a means of adjuvant therapy, which have a sensitizing effect to radiotherapy and chemotherapy. In recent years, the molecular biology, cell and animal experimental research of tumor thermochemotherapy progressed very quickly, which provide theoretical foundation and guidance for us to further develop hyperthermia combined with chemotherapy in clinical trials. In this paper, the studies with the mechanism of thermo-chemotherapy treatment of tumor, different ways of thermochemotherapy and commonly used drugs in thermochemotherapy are reviewed.