Social forces participating in non-profit medical institutions will help to insist on the commonweal of public medical and health care and realize the goal of access to basic medical and health services for everyone .At present , social forces participating in non-profit medical institutions have made some prominent improvements under a series of policy support .However , due to the absolute dominance of public hospitals , social forces participating in non-profit medical institutions are still potting a lot of restrictions on access threshold of basic medical services , devel-opment space and policy implementation .In order to promote the healthy development of social forces participating in non-profit medical institutions , we should first recognize its significance and guarantee its development potential , tal-ents construction and development space through implementing preferential policies , improving supervision mecha-nisms, exploring multi-sited license, optimizing allocation of resources and so on .

OBJECTIVE:To establish a RP-HPLC method for determination of the content of hydrochlorothiazide tablets.METHODS:Samples were determined by RP-HPLC on a ZORBAX SB-C 18 column with a mixture of phosphate buffer solution(pH=4.0)-methanol(80∶20)as mobile phase and UV detection at226nm.RESULTS:The calibration curves of hydrochlorothiazide was linear from0.006to0.303mg/ml(r=1.0000,n=5),the average recovery and relative standard deviation were100.1%and0.43%(n=9)respectively.CONCLUSION:The method is rapid,simple,accurate and precise.It can be used for assay of hydrochlorothiazide.

Objective To evaluate the neuroradiological features of intraventricular central neurocytoma in ten patients.Methods Ten patients with central neurocytoma were imaged using contrast-enhanced and non-enhanced MRI scans,including 4 were also scanned with non-enhanced CT.The imaging data were studied with regard to location,size,margin,signal intensity,enhancement characteristics and presence of calcifications.Clinical data(i.e.presenting signs and symptoms,physical findings and medical history) were collected and histopathological and immunohistochemical studies were performed.Results There were 4 male,6 female in our group.All lesions were located in the lateral ventricles.Three tumors were confined to the left side,three to the right side and four involved both lateral ventricles.The growth of central neurocytoma was of close spatial relation to the septum pellucidum.In MRI,most of the cases showed a heterogeneous hypointensity on T1WI and hyperintensity on T2WI or FLAIR with a well-defined margin.The presence of cystic components,necroses and calcifications had been the cause of these internal heterogeneities.After intravenous administration of gadolinium(Gd-DTPA) all tumors showed a heterogeneous enhancement.CT provided additional information by distinguishing intratumoral calcifications in all four evaluated cases.Conclusion Central neurocytoma should be considered when young patients with lesions in the lateral ventricle,which contain calcifications and show some enhancement.This is especially applicable for tumors involving both lateral ventricles with symmetrical growth around the center of septum pellucidum or for unilateral ventricular tumors with a wide base attachment to the septum pellucidum.

Objective To explore the correlation between serum homocysteine (Hcy) level and diabetes mellitus combined with cerebral infarction (DMCI) in patients. Methods A total of 144 patients with type 2 diabetes mellitus (T2DM) were selected and divided into two groups, patients without cerebral infaction (group A, n=64) and patients with cerebral infaction (group B, n=80). Thirty healthy people were used as control group (group C). The serum Hcy level was detected by enzymatic cycling assay in three groups. The serum levels of cholesterol (TC) and triglyceride cholesterol (TG) were detected by enzymatic determination. The serum levels of high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were detected by homogeneous phase method. The serum level of creatinine (Cr) was detected by creatine oxidase method. The level of uric acid (UA) was detected by urinary enzyme peroxidase coupling method. Glycosylated hemoglobin (HbA1c) was detected by high performance liquid chromatography. The carotid artery intima media thickness (IMT) was examined by color Doppler ultrasound, and patients were divided into mild and no stenosis group, moderate stenosis group and severe stenosis group according to the
results. The levels of Hcy were compared between all groups. The correlation of Hcy level and other indicators was analyzed. Results The levels of Hcy and HbA1c were group C0.05). There were no significant differences in UA and Cr levels between the three groups (P>0.05). The level of Hcy was positively correlated with age (rs=0.411), HbA1c (rs=0.219) and Cr (rs=0.242), and negatively correlated with gender (rs=-0.202) and HDL-C (rs=-0.278, P<0.05). There were no significant correlation between Hcy level and other variables (P>0.05). With the increased degree of carotid artery stenosis, the Hcy level and the proportion of HHcy showed a rising trend in patients (P<0.01). The level of Hcy was positively correlated with IMT(rs=0.781, P<0.001). Conclusion Hcy is a risk factor for the onset of DMCI. The high level of Hcy is closely related to the occurrence and progression of atherosclerosis. Hcy has great value for early screening and prevention of DMCI.

Aged ; Humans ; Male ; Sarcoma ; pathology ; Thyroid Neoplasms ; pathology

Objective To investigate the efficacy of family therapy in the treatment of adolescent depression and improvement of social performance.Methods 86 adolescent depressive patients were divided into drug therapy group(group A,n =43) and drug combined with family therapy group (group B,n =43) according to the order of inclusion.They were all treated for 12 weeks.Before and after the treatment,they were all evaluated with Hamilton Depression Rating Scale(HAMD-24),Personal and Social Performance scale (PSP) and Youth Self-Report scale(YSR).The changes of scores were compared with each other.Results The average scores of HAMD,PSP and YSR in group B before and after treatment was (27.30 ± 4.60) (81.47 ± 1.87) (73.28 ± 1.30) and (15.36 ±3.60) (12.61 ± 2.10) (9.46 ±2.80) (6.30 ± 2.20),(82.76 ± 1.09) (84.51 ± 1.32) (86.51 ±1.32) (92.05 ± 1.72),(71.42 ± 1.45) (69.09 ± 1.33) (67.47 ± 1.47) (64.23 ± 1.90) respectively.The average scores of HAMD,PSP and YSR in group A before and after treatment was (26.90 ± 5.10) (81.45 ± 1.85)(73.26 ± 1.28)and (19.42 ±2.90)(16.43 ±2.60)(13.50 ±3.80)(7.60±2.80),(82.00 ±1.14) (83.67 ±1.23) (85.88 ± 1.27) (87.98 ± 1.35),(72.84 ± 1.29) (70.07 ± 1.26) (68.84 ± 1.54) (66.02 ± 1.55) respectively.The difference of the scores after two-week treatment was significant statistically (P＜ 0.05).The difference of the deduction rate of the scores after four-week treatment was significant statistically (P ＜ 0.05).Conclusion Family therapy has better and faster efficacy in the treatment of adolescent depression,and it can significantly improve their social performance.

Objective To investigate the effect of ursodesoxycholic acid (UDCA) on JAK/STAT3/ COX-2 signal pathway in human hepatocellular carcinoma cells and the underlying mechanism of its antitumor effect.Methods In order to obtain the optimal concentration and time of UDCA,MTT assay was used to detect anti-proliferative activities of UDCA on SMMC-7721 cells induced by IL-6 in vitro.Cells were divided into control group,IL-6 group,AG490 (JAK2 inhibitor) group and UDCA group.The protein expressions of STAT3,p-STAT3 and COX-2 were measured by Western blot.Results MTT assay showed UDCA had obvious anti-proliferative activities on SMMC-7721 cells.The optimized concentrations were 50,100,200 μg/ml,the optimized culture time was 48 h.Western blot results showed that the expression of p-STAT3 and COX-2 were remarkably increased in IL-6 group than that in control group.There was no difference of STAT3 expression between the two groups.Compared with IL-6 group,the protein level of COX-2 was decreased in AG490 and UDCA groups,so was the expression of p-STAT3.But there was no change on STAT3 expression in AG490 and UDCA groups.Conclusion UDCA may decrease the proliferation of SMMC-7721 cells via inhibiting phosphorylation of STAT3 and down-regulating COX-2 exprcssion.

Objective To investigate the clinical efficacy of hemodialysis (HD) combined with hemoperfusion (HP) in the treatment of uremic pruritus.Methods The clinical data of 96 patients with uremic pruritus were analyzed retrospectively.All cases were treated with HD combined with HP.Results Pruritus score before and after treatment was (9.3 + 0.7) scores and (4.9 + 0.6) scores,respectively.And there was statistical significance (P ＜0.05).The erum creatinine,urea nitrogen,parathormone and β2 microglobulin before treatment were (712 ±148) μmol/L,(17.7 ±3.8) mmol/L,(47.1 ±12.4) ng/L,( 27.4 + 5.6 ) μ mol/L,respectively,and after treatment they were ( 324 ± 84 ) μ mol/L,( 7.4 ± 2.3 ) mmol/L,(15.6±5.1) ng/L,(11.1 ±2.2) μmol/L,there were statistical significance (P＜0.05).Conclusions HD combined with HP can effectively lighten the pruritus.It is worthy of clinical application.

Objective To explore the value of electroencephalogram (EEG) in children with headache.Methods 180 children with headache were carried out EEG recording,and the electrical activity of the brain was recorded and analyzed.Results In 180 cases of children,EEG was normal in 56 cases,abnormalities in 124 cases,the abnormal rate was 68.89％.The mild abnormalities was 78 cases,in a proportion to 43.33％ in the total record:moderate abnormalities was 14 cases,in a proportion to 7.78％ in the total reccrd;high-grede,abnormalities was 2 cases,in a proportion to 1.11％ ;5 patients was in critical state,in a proportion to 2.78％ ;epileptiform activity was 25 cases,in a proportion to 13.89％.97 normal cases with intermittent headache,abnormal rate was 46.1 1％,which was significantly lower than the attack headache ( x2 =15.16,P ＜ 0.05 ).Conclusion Headache in children with EEG examination had important significance.

Objective To study the apoptosis-inducing effect of histamin-indomethacin（his-IN）on Lewis lung cancer cell in mice.Methods The suspension of tumor cells from Lewis lung cancer mice was inoculated into the left axilla of Kunming mice.When the tumor grew to 1 cm in diameter,the mice were divided into 5 groups and received corresponding treatment：3.0 mg/kg indomethacin（IN）group,3.5 mg/kg IN group,3.0 mg/kg his-IN group,3.5 mg/kg his-IN group,and saline control group.Ten days later,the therapeutic effects were evaluated by the tumor inhibitory rate,the apoptotic peak and rate of tumor cells were measured by flow-cytometry（FCM）,and tumor necrosis factor α（TNF-α）level was estimated by radio-immunity method.Results TNF-α level,the tumor inhibitory rate and the apoptosis rate of his-IN group and IN group were significantly higher than those of the control group,and those of 3.5 mg/kg his-IN group was the highest.In the groups of his-IN or IN treatment,an apoptotic peak was found before G0/G1 phase,and the peak in his-IN group appeared earlier than IN group.Conclusion IN and his-IN could resist the growth of the tumor and promote cell apoptosis of Lewis lung cancer in mice.