Objective:To investigate the molecular mechanisms of proliferation and apoptosis by penta-brominated Diphenyl Ethers through regulation of mRNA and protein expression of Bcl-2 and Bax in breast cancer MCF-7 cells.Methods:Human breast cancer MCF-7 cells were grown in DMEM medium,MTT assay was employed to investigate cell proliferation.RT-PCR and immunohistochemistry was performed to determine mRNA and protein expression of Bcl-2 and Bax,Results:Within the range of 1?(10~(-8)～10~(-4))mol/L,5-BDEs stimulated proliferation in MCF-7 cells with a dose-dependent and time-dependent fashion.RT-PCR and immunohistochemistry analyses revealed that treatment with 1?(10~(-8)～10~(-4))mol/L for 72h resulted in increased mRNA and protein expression of Bcl-2 and inhibition of Bax.Conclusion:5-BDE possessed estrogenic activity and could markedly stimulate proliferation in estrogen responsive breast cancer MCF-7 cells.Up-regulation of Bcl-2 mRNA and protein expression was suggested to be involved in this biological effect.

Objective To investigate the effect and function analysis by sulfentanyl in patients with tongue cancer postoperative analgesia.Methods A total of 74 patients with radical resection of tongue cancer in department of anesthesiology with general anesthesia from our hospital were collected, patients or their families signed consent, according to postoperative self-controlled intravenous analgesia drugs divided into experimental group and control group with 37 cases in each group.Patients in control group were treated by fentanyl self-controlled intravenous analgesia;patients in experimental group were treated by sulfentanyl self-controlled intravenous analgesia, determination of the analgesic effect, sedative effect and the changed of vital signs postoperative 2, 4, 8, 12, 24, 48 h,at the same time recorded complications.Results After operation, at different time points analgesic effect and sedative effect were changed in the two groups, the VAS scored of the experimental group was lower than the control group postoperative 2, 4, 8, 12, 24, 48 h time points, the Ramsay Sedation scored of the experimental group was lower than the control group postoperative 2 h, 4 h, 8 h, 12 h time points ( P <0.05 ); the changed of vital signs and adverse reactions between the two groups had no difference within 48 hours ( P >0.05 ) . Conclusion Sulfentanyl in the treatment of patients with radical resection of tongue cancer the analgesic effect and sedative effect are better than fentanyl, has no effect on the vital signs, and the role is security and exact.

OBJECTIVETo investigate the effects of closed reduction and ulna intramedullary nail fixation for the treatment of fresh Monteggia fractures of Bado type I and II in children.

METHODSTwenty-three children patients with Monteggia fracture during July 2010 to September 2013 were treated by closed reduction and ulna intramedullary nail fixaion including 18 boys and 5 girls with an average age of 9.3 years old ranging from 6 to 13 years old. Among them,15 cases were Bado type I and 8 cases were Bado type II. There were 9 cases with radial nerve injury. The operation time,the recovery of nerve injury, the fracture healing and the function of elbow were observed and recorded.

RESULTSAll patients were followed up for 6 to 24 months (12 months on average). All patients were obtained bone healing. According to Anderson standard, at the final follow-up, 20 cases got excellent result, 2 cases got good result, and one case got fair result.

CONCLUSIONTreatment of the fresh Monteggia fractures in children by closed reduction and ulna intramedullary nail fixation has advantages of simple operation, less trauma and good results.

Adolescent ; Child ; Female ; Fracture Fixation, Intramedullary ; methods ; Fracture Healing ; Humans ; Male ; Monteggia's Fracture ; surgery ; Ulna ; surgery

Allergens ; analysis ; Humans ; Hypersensitivity ; diagnosis ; immunology ; Practice Guidelines as Topic ; Skin Tests

OBJECTIVETo evaluate the effect of repeated sintering on the color and translucency of dental lithium disilicate-based glass ceramic.

METHODSThirty disc specimens (10 mm in diameter, and 1.00 mm ± 0.01 mm in thickness) were fabricated using an IPS e.max Press HT A2 ingot and then randomly divided into six groups (SO, S1, S2, S3, S4, and S5) (n = 5). Each group was sintered 0, 1, 2, 3, 4, and 5 times individually according to the manufacturer's recommendation. After polishing, ultrasonic cleaning, and drying, the color parameters (L*, a*, and b* values) and transmittance (τ) of all the specimens were measured using a ShadeEye NCC dental colorimeter and a TM-2 spectrophotometer, respectively. Subsequently, C*ab and ΔE were calculated. The results were statistically analyzed using SPSS 17.0 software for ANOVA and LSD.

RESULTSAfter repeated sintering, the L* value significantly decreased (P < 0.05), and the a*, b*, and C*ab values initially increased and then decreased (P < 0.05). The color parameters (L*, a*, and b*) and the C*ab values of group S3 were higher than those of the other groups. The τ values initially increased and then decreased (P < 0.05), and these values were optimal when sintered twice. In terms of transmittance (τ), statistical differences existed between the following groups: S0 and S2, S0 and S3,.S2 and S5, and S3 and S5 (P < 0.05). Compared with group S0, the color differences ΔE were 0.89 minimally and 2.01 maximally after different sintering times, which can be clinically acceptable.

CONCLUSIONRepeated sintering can affect the color and translucency of the IPS e.max Press lithium disilicate-based glass ceramic, but the color difference can be clinically acceptable.

Ceramics ; Color ; Colorimetry ; Dental Materials ; Dental Porcelain ; Random Allocation ; Spectrophotometry

Objective Analyse the causes,diagnosis and treatment for delayed hemorrhage after distal radical gastrectomy.Methods Retrospective study on 45 patients combined with intro-abdominal hemorrhage after distal radical gastrectomy from January 2008 to June 2013.Results Thirteen patients combined with delayed hemorrhage in these 45 patients,all of 13 patients had intro-abdominal hemorrhage in 1week to 4 weeks after operation.And 5 of the 13 patiens were intermittent intro-abdominal hemorrhage 1 week after operation,these patients were demonstrated the blood come from gastroduodenal artery pseudoaneurysm fracture by CT and DSA examine,and they were cured by interventional embolization.Other 8 patients were marginal ulcer hemorrhage diagnosed by gastroscope,and they stoped bleeding with the help of gastroscope.Conclusions The causes of delayed hemorrhage after distal radical gastrectomy were complicated,and CT,DSA and endoscope can use for diagnosis.What was more,interventional embolization and endoscope were helpful for curing the intro-abdominal hemorrhage,avoiding re-operation.

The incidence rate of erectile dysfunction (ED) is reportedly as high as 30-90% after radical prostatectomy for prostate cancer, which seriously affects the patients' quality of life. Penile rehabilitation is defined as the use of any drug or device at or after radical prostatectomy to maximize erectile function recovery. A variety of treatment options are available for post-prostatectomy ED patients, including oral phosphodiesterase 5 inhibitors (PDE5I) , intracorporal injections, vacuum erection device, and penile prosthesis. This article presents an overview of the currently used methods for the drug treatment and penile rehabilitation of the ED patients after radical prostatectomy. It seems proper to recommend daily use of a vacuum erection device plus oral PDE5I in the early postoperative period. For those who fail to respond to this therapy, intraurethral alprostadil, intracorporal injections, or a penile prosthesis could be considered.
Alprostadil ; administration & dosage ; Combined Modality Therapy ; Erectile Dysfunction ; etiology ; therapy ; Humans ; Male ; Penile Erection ; Penile Prosthesis ; Phosphodiesterase 5 Inhibitors ; administration & dosage ; Prostatectomy ; adverse effects ; Quality of Life ; Recovery of Function ; Vacuum

Objective To investigate and analysis the prevalence rate of hospital infection and community infection,and to reduce the incidence of hospital infection.Methods First we investigated hospitalized patients in September 9,2013 by two ways which in-clude bedside investigation and reviewing the medical records;and then described the prevalence case of infection and the usage of antimicrobial agent by descriptive approach.Results Seven hundred and seventy patients were investigated with the rate of 100.00%;18 patients were hospital infection cases,the infection rate was 2.34%;most of the hospital infection cases was occur in lower respiratory tract;228 patients were community infection cases.The infection rate was 29.61%;the primary affected areas of community infection were respiratory and gastrointestinal tract;the structure of hospital infection pathogens were almost the same with the community infection,most of them were G- bacteria;186 patients were used antimicrobial agent in that day,the usage rate was 24.16%.Conclusion Promoting the managements of high risk departments and susceptible affected areas,making relevant rules of operation are the focus of our works in the future.

[Objective]To examine the mechanism and clinical outcome of the traction reduction method in treating the adult shoulder dislocation.[Method]From January 1997 to August 2007,68 patients diagnosed with shoulder-dislocation received reduction with this method.A retrospective and observational study of the outcomes was assessed in a number of patients.There were altogether 68 patients,51 male and 17 female,with an average age of 49(ranging from 16-82 years old).Forty of the cases occurred in the right shoulder and 28 others in the left,all caused by trauma:15 in traffic accidents,32 by injuries from falls,and 21 by sports injuries.Forty of the dislocations lied under the processus coracoideus,17 under the glenoid,6 under the collar bone and 5 post dislocation.Seventeen of the patients had recurrent dislocation,13 with nodules avulsion fracture of the humerus,2 with brachial plexus injury,3 with surgical neck of humerus fractures.The shortest time of restoration after injury was 30 minutes,and the longest 10 days,with an average of 2.1 days.[Result]Patients that had received the reduction method for 30 seconds to 5 minutes were all recovered with one treatment,the result being confirmed by X-rays.Sixty-four of the patients were followed up for 6-24 months and the clinical results were:61 cured and 3 turned to the better.[Conclusion]This method is easy and flexible in the reduction of adult shoulder dislocation,with fewer complications than other traditional methods.It accords with the shoulder anatomy and principles of bio-mechanics,and it is suitable to popularizing.

Objective To determine the changes of coagulation indexes of rabbits struck at different levels and sieve the traditional coagulation indexes, such as activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and fibrinogen (FIB). Methods We used correlation analysis to precondition the data to reject indexes, and used stepwise discriminatory analysis to choose indexes and made the diagnosis by multiple linear discrimination analysis. Then, by random sampling we compared the correct diagnostic rate by 4 indexes with that by 3 indexes. Results The correct discrimination rate of blood coagulation function diagnosis in rabbits was up to 87.60% by the four indexes and 96.12% with the three indexes (APTT, PT and TT). The average correct discrimination rate of blood coagulation function diagnosis in 1 000-time random sampling was up to 83.07% by the four indexes and 85.16% by the three indexes. Conclusion Stepwise discriminatory analysis can be used to discriminate hemorrhage from thromboxane. The correct diagnostic rate gets higher when FIB was rejected.