Objective:To analyze the clinical effect and influencing factors of closed reduction and percutaneous cannulated screw fixation in the treatment of femoral neck fracture in the adolescents, and to provide evidence for surgical treatment of femoral neck fracture in the adolescents. Methods:The clinical effect and influencing factors of 36 cases of femoral neck fracture treated by closed reduction and percutaneous cannulated screw fixation were analyzed. The time of operation, the time of fracture healing evaluated with X-ray image, the evaluation on the function after operation by Harris score of hip joint,and the occurrence of complications of the patients were observed.Results:All the cases were followed up for 6-24 months, average 16 months. One case of all patients had avascular necrosis of the femoral head, and accounted for 2.78% of all the patients;2 cases of all fractures were nonunion, and 5.56%.The remaining 33 cases were completely healed.The HHS-harris hip score results showed that 26 were excellent, 6 cases were good, and 2 cases were poor;the excellent and good rate was 88.89%.Conclusion:Closed reduction and percutaneous cannulated screw fixation in the treatment of adolescent femoral neck fracture is an effective program of operation;timing of operation, good reduction, and rigid internal fixation can decrease the femoral head necrosis and the incidence of complications.

This study was aimed to observe the effect of Bushen-Huoxue-Tonglin formula (BSHXTLF) on Ki-67 and apoptotic bodies in benign prostatic hyperplasia (BPH) rats. A total of 72 Wistar rats were randomly divided into 6 groups, which were the sham operation group, model group, Proscar group (0.8 mg?kg-1), BSHXTLF groups with low, middle and high dose (7.5, 15, 22.5 g?kg-1). Except the sham operation group, the BPH model was established by injecting testosterone propionate (5 mg?kg-1) for 30 days after removing both testicles in the rats. The drugs were administrated once a day for 30 days at the same time. The immunohistochemical and TUNEL methods were used to determine Ki-67 and apoptotic bodies, respectively. The results showed that compared with the model group , the expression of Ki-67 in treatment groups was decreased ( P < 0 . 05 ) . Compared with Proscar group and low-dose BSHXTLF group, the expression of Ki-67 in the middle- and high-dose BSHXTLF group was decreased ( P < 0 . 05 ) . Compared with the middle-dose BSHXTLF group , the expression of Ki-67 in the high-dose BSHXTLF group was decreased ( P < 0 . 05 ) . Compared with the model group , the expression of apoptot-ic bodies in each treatment group was increased ( P < 0 . 05 ) . Compared with Proscar group , low-dose and middle-dose BSHXTLF group, the expression of apoptotic bodies in the high-dose BSHXTLF group was increased (P <0.05). It was concluded that BSHXTLF may decrease the expression of Ki-67 and increase the expression of apoptotic bodies in BPH rats. These results provided objective evidences for clinical treatment of BPH.

Objective:To evaluate the influence of examination as a transient stressful life event on the psychological status and occlusal function of college students.Methods:47 student volunteers(17 males,30 females;aged 19.30 ± 1.53 years) with normal occlusion were enrolled.SCL-90 questionnaire for evaluation of psychological status and surface electromyography for assessment of masseter and temporalis activities in maximum voluntary teeth clenching and in chewing different foods were performed at two stages (stage 1:one week before final academic examination;stage 2:one week after spring festival holiday).Results:Scores of questionnaire evaluation of psychological status showed obvious change in factors of somatization,interpersonal sensitivity,depression,anxiety and paranoid ideation(P ＜0.05).Paired t-test analysis of masticatory muscle function showed changes only in percentage overlapping coefficient(POC) of masseters in chewing carrot and activity index(AC) in chewing preserved haw jelly(P =0.014 and 0.018 respectively).However,other chewing status and indexes were not significantly changed.Conclusion:The psychological status of college students can be significantly impacted by examination stress without influence on masticatory activities.

The β-sheet peptides can be self-assembled to form different supramolecular solids. The supramolecular solid can be linked to a wide range of functional domains, for example, with cell adhesion sequences, signal domains, and vaccine epitopes to form complex nanostructures, which can be widely used in biomedical fields. In this paper, we mainly reviewed the self-assembly of peptides using β-folding secondary structure to form nanostructures, and discussed the application of nanostructures in drug delivery and tissue engineering.

[ ABSTRACT] AIM: To investigate the protective effect of mesenchymal stem cell ( MSC)-conditioned medium (MSCCM) on myocardial cell line H9c2 and its mechanism.METHODS:Verification of MSC was performed by flow cy-tometry analysis, followed by MTT assay to determine the optimal incubation time of MSCCM with myocardial cells.The cells were divided into 4 groups:normal ( N) group, model ( M) group, M+MSCCM group and MSCCM group.The cells in M+MSCCM group and MSCCM group were pre-incubated with MSCCM for 24 h.The cells in M group and M+MSCCM group were treated with 300 μmol/L H2 O2 for 4 h to imitate oxidative injury of myocardial cells.Mitochondrial membrane potential and apoptotic rate of injured myocardial cells were detected by flow cytometry.The ROS production was measured by fluorescence microscopy.The nuclear translocation of Nrf2 and expression of HO-1 was examined by Western blot.RE-SULTS:No difference of mitochondrial membrane potential, apoptotic rate or ROS production between MSCCM group and N group was observed (P>0.05).The mitochondrial membrane potential depolarization, apoptotic rate and ROS produc-tion in M+MSCCM group were significantly lower than those in M group ( P<0.01 ) .The nuclear translocation of Nrf2 and expression of HO-1 in the myocardial cells were increased with MSCCM incubation time prolonged.CONCLUSION:MSCCM protects the myocardial cells against oxidative injury induced by H2 O2 .The anti-oxidative mechanism would be as-sociated with the activation of Nrf2/ARE pathway.

Aim To investigate the protective effect of isorhamnetin on H9 C2 myocardial cell line and its mechanisms. Methods The toxicity and optimal pro-tective concentration of isorhamnetin were determined by MTT assay. The experimental subjects were divided into four groups:group N ( normal ) , group M ( mod-el) , group M + ISO ( model + isorhamnetin ) , and group ISO ( isorhamnetin only ) . Group M +ISO and ISO were pre-incubated with isorhamnetin for 12 hours while other groups with plain DMEM. Group M and M+ ISO were treated with 300μmol · L-1 H2 O2 for 4 hours after pre-incubation. Mitochondrial membrane potential depolarization of H9 C2 was measured by fluo-rescence microscope. Apoptotic rate and ROS produc-tion of injured myocardial cell line were detected using
flow cytometry. The oxidative indictors were measured by spectrophotometry. The expressions of cytoplasmic cytochrome C, caspase-9, caspase-3, Bcl-2, Bax, Nrf2 and HO-1 were examined by Western blot. Result There was no difference in mitochondrial membrane potential depolarization, apoptotic rate, ROS produc-tion, oxidative indictors production and expressions of cytoplasmic cytochrome C, caspase-9,caspase-3, Bcl-2 , Bax between groups ISO and N ( P>0. 05 ) . Apop-totic rate, ROS production, expressions of cytoplasmic cytochrome C, caspase-9, caspase-3, Bax, MDA pro-duction of group M+ISO were significantly lower than those of group M ( P < 0. 01 ) . And mitochondrial membrane potential, Bcl-2, CAT, SOD and GSH-Px of group M + ISO were increased compared to group M .
Nuclear translocation of Nrf2 and expression of HO-1 in myocardial cell line were increased with the prolonged isorhamnetin incubation time. Conclusion Isorham-netin could protect myocardial cell line against H2 O2-induced oxidative injury and apoptosis through the in-
terruption of mitochondrial dependent apoptotic path-way and activation of Nrf2/ARE pathway.

This study was aimed to observe the effect of Bu-Shen Huo-Xue Tong-Lin (BSHXTL) formula on gene expression of Caspase-3 in benign prostatic hyperplasia (BPH) rats. A total of 72 rats were randomly divided into the sham operation group, model group, proscar group, low-dose BSHXTL group, middle-dose BSHXTL group and high-dose BSHXTL group. The rat model was established by injecting testosterone propionate for 30 days after removing testis except the sham operation group. The drugs were administered once a day at the same time of the model establishment. Immunohistochemical method was used to measure positive average gray. And RT-PCR was used to measure the gene expression of Caspase-3.The results showed that compared with the model group, the expression of Caspase-3 and mRNA in each treatment groups were increased (P < 0.05). And the high-dose BSHXTL group was higher than the proscar group and low-dose BSHXTL group (P< 0.05). It was concluded that BSHXTL formula may upregulate gene expression of Caspase-3, which may be the mechanism of BPH treatment.

Humans ; Hypertension, Pulmonary ; diagnosis

BACKGROUND:Previous studies have found that platelet-rich fibrin has a good ability to induce gingival soft tissue repair and regeneration.
OBJECTIVE:To observe the effects of platelet-rich fibrinversus colagen membrane on gingival soft tissue healing, and to evaluate the ability of platelet-rich fibrin to repair gingival defects.
METHODS:Twenty-two patients (2 premolar teeth and 20 molars) scheduled for premolar or molar removal or ridge preservation due to various reasons were selected and randomized into two groups. Bio-Oss was implanted into the extraction socket folowed by covering with platelet-rich fibrin or colagen membrane. Healing time and healing rate of gingival defects were detected to evaluate the ability of platelet-rich fibrin to promote gingival tissue healing at 1-2 weeks after Bio-Oss implantation.
RESULTS AND CONCLUSION:The healing time was (12.17±2.25) days in the platelet-rich fibrin group and (17.30±2.58) days in the colagen group. The healing rate of the platelet-rich fibrin group was notably higher than that in the colagen membrane group at 1 and 2 weeks after Bio-Oss implantation. These findings indicate that platelet-rich fibrin is better than colagen membrane to improve the healing of gingival soft tissues with a shorter healing time.

BACKGROUND:Under co-culture conditions, mesenchymal stem cel s could regulate osteogenic differentiation and osteogenesis of osteoblasts. OBJECTIVE:To observe the osteogenic efficiency of osteoblastic precursor cel s co-cultured with undifferentiated bone marrow-derived mesenchymal stem cel s, umbilical cord-derived mesenchymal stem cel s, or placenta-derived mesenchymal stem cel s in mineralization medium. METHODS:Adipose-derived stem cel s were induced in osteogenic differentiation medium for 7 days before being indirectly co-cultured with undifferentiated mesenchymal stem cel s isolated from different tissues (bone marrow group, umbilical cord group and placenta group) in Transwel plates. Induced adipose-derived stem cel s cultured alone served as control group. At different experimental intervals, quantitative analysis of alkaline phosphatase activity and calcified matrix was preformed to observe the effects of mesenchymal stem cel s from different sources on the osteogenic efficiency of induced adipose-derived stem cel s. RESULTS AND CONCLUSION:Expression of alkaline phosphatase was significantly higher in different experimental groups than the control group (P<0.05), and it was also higher in the bone marrow group than the umbilical cord and placenta groups (P<0.05). Quantitative analysis of calcified matrix revealed that the experimental groups were significantly higher than the control group (P<0.05);and in experimental groups, the umbilical cord group was higher than bone marrow group and placenta group (P<0.05). These findings indicate that the osteogenic efficiency of induced adipose-derived stem cel s is improved dramatical y under co-culture conditions.