Cytoskeletal Proteins ; biosynthesis ; genetics ; physiology ; Humans ; Neoplasm Metastasis ; Neoplasms ; pathology ; Signal Transduction

Humans ; Mining ; Noise, Occupational ; statistics & numerical data ; Occupational Exposure ; statistics & numerical data ; Work Schedule Tolerance

Objective To assess the effect of thoracoscopic surgery under laryngeal mask anesthesia and explore the nursing strategies. Methods Thirty-five patients from April to December in 2014 undergoing video-assisted thoracoscopic surgery (VATS) were given intubation anesthesia and another thirty-five ones undergoing the same surgery in 2015 received laryngeal mask anesthesia. The two groups were compared in terms of surgery conditions, surgery complication and ambulation time. Results No significant differences were found in surgery time or blood loss between two groups (P>0.05). The postoperative waking time in the mask anesthesia group was significantly shorter than that of the intubation anesthesia group (P<0.05), and ambulation time was significantly shortened (P<0.05). The rates of throat discomfort, hoarseness and gastrointestinal reactions were significantly lower. Conclusions Laryngeal mask anesthesia used in small thoracoscopic surgery for airway management is safe and feasible. Combined with training in respiration and limb function, better analgesia and nursing, it can keep away complications related to intubation anesthesia, shorten hospital stay and accelerate postoperative rehabilitation.

Objective To investigate the clinical efficacy of electrothermal acupuncture in treating ankylosing spondylitis.Method Sixty patients were randomly allocated to treatment and controlgroups, 30 cases each.The treatment group received electrothermal acupuncture and the control group took methotrexate plus sulfasalazine.The extent of chestexpansion, the severity of sacroiliac joint tenderness, the number of tender joints and morning stiffness time were examined,Schober's testscores at different time points were recorded and interleukin-1 (IL-1) and interleukin-6 (IL-6) contents were measured before and after 4 and 8 weeks of treatment.Result In the treatment group, there were statistically significant differences in the extent of chestexpansion, the severity of sacroiliacjoint tenderness, the number of tender joints, morning stiffness time andSchober's testscores after 4 and 8 weeks of treatment compared with before treatment (P<0.05).There were statistically significant pre-/post-treatment differences in IL-1 and IL-6 contents in the two groups (P<0.05). The total efficacy rate was 80.0% in the treatment group and 73.3% in the control group; there was no statistically significant difference between the twogroups(P>0.05). ConclusionElectrothermal acupuncture has a marked therapeutic effect onankylosing spondylitisand is of high safety.The mechanism of electrothermal acupuncture treatment forankylosing spondylitismay be related to its regulating IL-1 and IL-6 levels in the body.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Central Nervous System Neoplasms ; drug therapy ; Female ; Follow-Up Studies ; Humans ; Leukemia, Promyelocytic, Acute ; drug therapy ; Male ; Middle Aged ; Prognosis ; Retrospective Studies ; Young Adult

Objective To study the mechanism that glial cell line-derived neurotrophic factor (GDNF)promotes human glio-ma cells proliferation.Methods We divided glioma samples into two groups,including low-grade glioma group and high-grade glio-ma group,while cerebral contusion patients were treated as the control group,12 cases in each group.C6 glioma cell lines were di-vided into three groups,such as GDNF group,BSA(bovine serum albumin)group and control group.CCK-8 (cell counting kit-8) was used to detect the cell proliferation,while Western blot was used to detect the expression of AKT,p-AKT,β-catenin and p-β-catenin in each group.Results Comparing with the control group,the expression levels of AKT,p-AKT,β-catenin and p-β-catenin in glioma group had a significantly increased (P <0.05).Meanwhile,the high-grade gliomas group also had a significant increase in those more than low-grade gliomas group (P <0.05).CCK-8 test showed that the cell proliferation in GDNF group was significant-ly higher than the control group (P <0.05),and the expression levels of p-AKT,β-catenin and p-β-catenin proteins all had a signifi-cant increase (P <0.05).However,the expression level of AKT had no obvious difference.Conclusion GDNF might promote the proliferation of glioma cells by up-regulating the expression of p-AKT,β-catenin and p-β-catenin.

Objective To investigate the anti-H5N1 activities and chemical constituents from stems and leaves of Buddleja lindleyana Fort.. Methods Constituents were separated through AB-8 macroporous resin, chromatography of silica gel, Sephadex LH-20 and recrystallization. Structures of the compounds were identified by analysis of physicochemical properties and spectral data. Results Ten compounds were isolated and identified as linarin(1), rutin(2), luteolin(3), quercetin(4), apigenin(5), hesperetin (6), salidroside (7), oleanolic acid (8),β-sitosterol (9), and daucosterol (10), respectively. Conclusion Compounds 2-7 were obtained from this plant for the first time.

Objective:To investigate the blood lipid status and its variation regularity of a population of teaching staff in order to provide evidence for preventing hyperlipidemia.Method:Lipid indexes such as TC,Triglyceride(TG),LDL-C and HDL-C are inspected of a group of teaching staff over 40 years old in some certain university,and data statistics and analysis are conducted.Conclusion:Main behaviors of male and female teaching staff's hyperlipidemia are high TG and high LDL-C.Special focus should be put on blood lipid's variation level of middle aged males around 40 years old,periodical clinical monitoring should be done for female teaching staff over 50 years old.Medical information instruction on the prevention and control of high blood lipid should be strengthened in order to realize the unity of self control and social control.

Objective To observe the expression pattern of caspase-3 and HCLS1-associated protein X-1 (HAX-1) at different time after cerebral contusion in rat, and explore the new method for estimating the injury interval. Methods The cerebral contusion model was established using adult SD male rats. Then the rats were randomly allocated into 8 groups: 2 h, 6 h, 12 h, 1 d, 3 d, and 7 d after cerebral con-tusion, sham-operation and normal control. Expression of caspase-3 and HAX-1 protein after cerebral contusion in rat was detected by Western blotting. Laser scanning confocal microscope was used to ob-serve the number of HAX-1 positive cells and TUNEL-stained cells after cerebral contusion. Results The expression of caspase-3 increased parallelly with the time after cerebral contusion and reached the peak value on 3 d. The expression of caspase-3 decreased gradually and still maintained a high level expression on 7 d (P<0.05). The expression of HAX-1 positive cell went up after injury, and reached the peak value at 6 h (P<0.05), then turned down gradually after 12 h and went out of detection after 3 d. The number of TUNEL-stained cells increased obviously at 2 h and reached the peak value on 3 d. The number of TUNEL-stained apoptotic cells decreased gradually and still maintained a high level expres-sion on 7 d (P<0.05). Conclusion The expression of caspase-3 and HAX-1 after cerebral contusion has time sequential regularity, which may provide new evidence for forensic diagnosis of cerebral contusion interval.

Objective To investigate the neuroprotective effects and mechanisms of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/ IMP cyclohydrolase(AICAR) supplement (AMPK activator) in different stages of neonatal rats sufferring from hypoxia-ischemia encephalopathy ( HIE). Methods Neonatal rat hypoxia-ischemia brain injury model was employed in this study. A total of 160 neonatal rats were distributed into five groups: sham, model control,AICAR30 min, AICAR24 h and AICAR72 h. The neuroprotective effects of AICAR supplement (30 min, 24 h, 72 h post operation) were compared by cresyl violet staining; Expressions of P-AMPK,AMPK in the brain tissue were measured by Western blotting.Foot-faults method was used to evaluate the long-term prognosis of the rats. Results Compared with the sham group, the survival of rats brain in model control group was significantly decreased [(100.0± 0.1)% and (45.3± 6.3)%, P< 0.05]. AICAR had neuroprotective effects when treated at 30 min and 24 h post operation,while the protective effects disappeared when treated later (72 h post operation) (P>0.05). Compared with the sham group, the expression of P-AMPK significantly increased about three times, while ATP level decreased close to the same. Conclusion Early AICAR treatment can protect hypoxia-ischemia brain injury by increasing AMPK-ATP level.