1.Low-grade (fibromatosis-like) spindle cell carcinoma of the breast: case report and review of the literature.
Feng TANG ; Dong-hua GU ; Yun BAO ; Hong WANG ; Hong-guang ZHU ; Zu-de XU ; Xi-qi HU
Chinese Journal of Pathology 2005;34(7):444-445
Breast Neoplasms
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metabolism
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pathology
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surgery
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Carcinoma
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Female
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Fibroma
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metabolism
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pathology
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surgery
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Humans
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Keratins
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metabolism
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Middle Aged
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Vimentin
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metabolism
2.Effects of traditional Chinese medicine on the differentiation of mesenchymal stem cells to osteoblasts
Bao-Yong YAN ; Fu-Sheng DONG ; Jie WANG ; Fu-Liang HAO ; Yu-Ying DONG ; Qi-Hui HU
Chinese Journal of Stomatology 2010;45(1):31-35
Objective To investigate the effects of traditional Chinese medicine on the differentiation of mesenchymal stem cells(MSC)to osteoblasts.Methods Six male Beagle dogs weighed 10-15 kg each were divided into three groups,group A:medicine serum group,group B:non-medicine serum group and group C:bovine serum group.The serum of group A was obtained from the femoral artery of 2 Beagle dogs drinking equivalent dose of traditional Chinese medicine according to body surface area for 7 continuous days.The serum of group B was collected from the femoral artery of 2 Beagle dogs fed with equal volume of normal saline for 7 days.The serum of group C was fetal bovine serum.The tibia marrow was harvested from another 2 Beagle dogs and MSC were isolated and purified by density gradient centrifugation.MSC were cultured in DMEM solution with fetal bovine serum. After MSC were digested by trypsin.MSC were cultured in DMEM solution wuth the osteogeneic inducer,which contained dexamethasone,antiscorbutic and β-glycerophosphate.Morphological and histological changes of the MSC were observed under an inverted microscope.Alizarin monosulfonate and nitric acid argentum staining was performed to observe the calcium deposition.MSC were curtured in DMED solution with medicine serum(group A),non-medicine serum(group B)and bovine serum(group C)respectively.The growth curve was detected by the methyl thiazoly tetrazolium (MTT). The alkaline phosphatase(ALP) activities were detected to observe the differentiation of MSC. Results The original MSC were observed as fibroblast-like cell shapes. After the osteogeneic inducer was added, MSC were polygon cells with a few polyprocess. Calcium deposition appeared during 10-14 days and alizarin monosulfonate and Von Kossa staining presented positive. MTT results showed that the number of adherent cells of group A was more than that of group B and that of group C significantly after 6 days (P <0. 05). ALP detection proved that ALP activity of group A was more than that of group B and that of group C significantly after 5 days ( P < 0. 05 ). Conclusions The traditional Chinese medicine promotes the differentiation of MSC to osteoblasts and osteogenesis.
3.Three-dimensional finite element analysis of lumbar vertebra loaded by static stress and its biomechanical significance.
Jia-Can SU ; Zhuo-Dong LI ; Lie-Hu CAO ; Bao-Qing YU ; Chun-Cai ZHANG ; Ming LI
Chinese Journal of Traumatology 2009;12(3):153-156
OBJECTIVETo explore the mechanical behavior of lumbar spine loaded by stress and provide the mechanical basis for clinical analysis and judgement of lumbar spine fracture classification, mechanical distribution and static stress.
METHODSBy means of computer simulation method, the constructed lumbar spine three-dimensional model was introduced into three-dimensional finite element analysis by software Ansys 7.0. The lumbar spine mechanical behavior in different parts of the stress loading were calculated. Impact load is 0-8000 N. The peak value was 8000 N. The loading time is 0-40 minutes. The values of the main stress, stress distribution and the lumbar spine unit displacement in the direction of main stress were analyzed.
RESULTSThe lumbar spine model was divided into a total of 121 239 nodes, 112 491 units. It could objectively reflect the true anatomy of lumbar spine and its biomechanical behavior and obtain the end-plate images under different stress. The stress distribution on the lumbar intervertebral disc (L(3)-L(4)) under the axial, lateral flexion and extension stress, and the displacement trace of the corresponding processus articularis were analyzed.
CONCLUSIONIt is helpful to analyze the stress distribution of lumbar spine and units displacement in static stress loading in the clinical research of lumbar spine injury and the distribution of internal stress.
Adult ; Biomechanical Phenomena ; Female ; Finite Element Analysis ; Humans ; Lumbar Vertebrae ; physiology ; Sacrum ; physiology ; Stress, Mechanical
4.Disequilibrium linkage between the polymorphism in exons 2, 3 and 4 of the MICA gene and HLA-B antigen of patient with ankylosing spondylitis.
Hong SU ; Bao-long WANG ; Xiu-jun ZHANG ; Jia-hu HAO ; Qin XIAO ; Dong-qing YE
Chinese Journal of Medical Genetics 2006;23(4):446-448
OBJECTIVETo investigate the association between the exons 2 to 4 of the MICA gene and ankylosing spondylitis (AS).
METHODSBy PCR-SSOP, DNA samples from 56 AS patients and 112 random healthy individuals, as normal control were genotyped to analyse the polymorphism in exons 2, 3, 4 of the MICA alleles.
RESULTSMICA*008 was dominant in MICA allele,accounted for 32.14% and 30.36% in AS patients and normal controls respectively. The frequency of MICA*007 was significantly increased in AS patients, when compared with normal controls (chi-square=10.18, P<0.05, RR=2.50). No difference was found in the other MICA alleles. The haplotype analysis revealed that there were the strong linkage disequilibrium between MICA and HLA-B of AS patients, and normal controls. There was a difference in MICA*007-B27 between two groups (chi-square=18.46, P<0.05, RR=7.47). Both HLA-B27 and MICA*007 were strongly associated with AS. Stratified analysis showed that HLA-B27 was significantly relative to AS,while it was not found between MICA*007 and AS.
CONCLUSIONThe increased frequency of MICA alleles may be due to its strong linkage disequilibrium with HLA-B27.
Alleles ; Exons ; genetics ; Gene Frequency ; Genotype ; HLA-B Antigens ; genetics ; HLA-B27 Antigen ; genetics ; Histocompatibility Antigens Class I ; genetics ; Humans ; Linkage Disequilibrium ; genetics ; Polymerase Chain Reaction ; Polymorphism, Genetic ; genetics ; Spondylitis, Ankylosing ; genetics
5.Density functional theory investigation on antioxidation activity of four flavonoids from Rhododendri Daurici Folium.
China Journal of Chinese Materia Medica 2016;41(14):2675-2679
Four main flavonoids of the Chinese medicine Rhododendri Daurici Folium were studied using the density functional theory (DFT) B3LYP method with 6-311 + + G (d, p) basis set.Their activities were analyzed based on molecular structure, bond dissociation energy (BDE) and the energy gap between HOMO and LUMO. As a result, the antioxidant ability order of the four flavonoids compounds is farrerol
6.Development and study of three-dimensional CT scanning system for dental cast measurement and analysis.
Bin YAN ; Lin WANG ; Qiu-sheng HU ; Lin PAN ; Kui YANG ; Xu-dong BAO
West China Journal of Stomatology 2005;23(4):329-331
OBJECTIVETo develop a computer assisted CT scanning system for 3D dental cast measurement and to study its reliability.
METHODS20 dental stone casts were scanned by helical CT and captured a series digital sliced radiographs of them. The program which was developed by Visual C 6.0 language and run under the Windows surroundings rebuilt the 3D graphics and measured the coordinate of the dental cast. These dental casts were scanned and measured to test the reliability of the system, contrasting with manual measurement.
RESULTSThere was no significant difference between 3D CT scanning and manual measurement (P > 0.05).
CONCLUSIONThe system is a valuable computer assisted system for clinical diagnosis and treatment of malocclusion.
Dental Models ; Humans ; Image Processing, Computer-Assisted ; Imaging, Three-Dimensional ; Malocclusion ; Reproducibility of Results
7.CT and MRI image fusion in three-dimensional conformal radiotherapy for cranial carcinoma.
Jing YAN ; Jie LU ; Bao-Sheng LI ; Wan-Hu LI ; Dong-Liang HOU ; Juan LIU
Chinese Journal of Oncology 2007;29(12):935-937
OBJECTIVETo investigate the advantage of CT and MRI image fusion in determining the target precisely during 3-dimensional conformal radiotherapy for cranial carcinoma.
METHODSTwenty-five patients received CT and MRI examination simultaneously for localizing the tumor and defining target before 3-dimensional conformal radiotherapy. The target defined by MRI image was used as gross tumor volume, whereas CT value was used to calculate dose, making plan for radiotherapy. The difference between the target defined by CT and MRI was compared.
RESULTSAll the 25 patients underwent CT and MRI image fusion for localizing the tumor and defining the target in order to make anatomic symbol and surface symbol superposed. The number of tumor nodual detected by CT was as same as that found by MRI in 23 cases except two. Compared with the GTV defined by MRI image, it was larger in 10 cases by CT image, whereas smaller in 15 cases. The response rate assessed by MRI image was 64.0% (CR + PR) at the end of radiotherapy.
CONCLUSIONCT and MRI image fusion technique is more precise than either by CT or MRI alone in defining the GTV of 3-dimensional conformal radiotherapy for cranial carcinoma.
Adolescent ; Adult ; Aged ; Brain Neoplasms ; diagnostic imaging ; pathology ; radiotherapy ; Humans ; Imaging, Three-Dimensional ; Magnetic Resonance Imaging ; methods ; Middle Aged ; Radiotherapy Planning, Computer-Assisted ; Radiotherapy, Conformal ; methods ; Remission Induction ; Tomography, X-Ray Computed ; methods ; Tumor Burden ; Young Adult
8.Development of clinical practice guidelines for the management of adherence to highly active antiretroviral therapy
Liang FU ; Yan HU ; Hongzhou LU ; Meijuan BAO ; Lin ZHANG ; Yinzhong SHEN ; Lijun ZHA ; Cheng′en PAN ; Huiwen LI ; Zheng ZHU ; Ning DONG ; Yanjuan GAN
Chinese Journal of Practical Nursing 2016;32(19):1497-1501
Objective To develop the clinical practice guidelines for the management of medication adherence to highly active antiretroviral therapy (HAART) in China. Methods The development methods included qualitative interview of 31 stakeholders, questionnaire survey of 423 PLHIV, adaptation of 30 clinical practice guidelines related to AIDS care, and overviews of reviews of 44 systematic reviews/Meta-analysis. Results 10 clinical practice guidelines and 10 systematic reviews/Meta-analysis were included. The clinical practice guidelines for the management of HAART were formed. Conclusions The formed clinical practice guidelines showed better applicability and higher general quality. It is recommended to use the guidelines in AIDS care.
9.Effects of hyperfractionated radiotherapy versus hypofractionated radiotherapy combined with concurrent chemotherapy on prognosis of limited-stage small-cell lung cancer
Xiao HU ; Bing XIA ; Yong BAO ; Yujin XU ; Jin WANG ; Honglian MA ; Ying JIN ; Min FANG ; Huarong TANG ; Mengyuan CHEN ; Baiqiang DONG ; Xiaolong FU ; Ming CHEN
Chinese Journal of Radiation Oncology 2017;26(9):1000-1005
Objective To investigate the effects of hyperfractionated radiotherapy versus hypofractionated radiotherapy combined with concurrent chemotherapy on the prognosis of limited-stage small-cell lung cancer (SCLC).Methods A total of 188 patients with limited-stage SCLC were enrolled in this study and divided into hyperfractionated group (n=92) and hypofractionated group (n=96).The hyperfractionated group received thoracic radiotherapy at 45 Gy in 30 fractions twice a day, while the hypofractionated group received 55 Gy in 22 fractions once a day.The Kaplan-Meier method was used to calculate survival rates, and the Cox model was used for multivariate prognostic analysis.Results There were not significant differences in 1-, 2-, and 5-year progression-free survival (PFS) rates and 1-, 2-, and 5-year overall survival (OS) rates between the hyperfractionated group and the hypofractionated group (82% vs.85%, 61% vs.69%, 59% vs.69%, P=0.27;85% vs.77%, 41% vs.34%, 27% vs.27%, P=0.37).The multivariate analysis showed that the time from the initiation of chemotherapy to the initiation of thoracic radiotherapy ≤43 days was favorable prognostic factor for PFS (P=0.005).The time from the initiation of chemotherapy to the end of thoracic radiotherapy ≤63 days and prophylactic cranial irradiation were favorable prognostic factors for OS (P=0.044;P=0.000).There were significant differences in incidence rates of grade 2 and 3 acute radiation esophagitis between the two groups (28% vs.16%, 9% vs.2%, P=0.009).Conclusions Both hyperfractionated radiotherapy and hypofractionated radiotherapy combined with chemotherapy can improve the PFS and OS of patients with limited-stage SCLC.The time from the initiation of chemotherapy to the initiation of thoracic radiotherapy ≤43 days and the time from the initiation of chemotherapy to the end of thoracic radiotherapy ≤63 days are favorable prognostic factors for PFS and OS, respectively.However, the hyperfractionated group has significantly higher incidence rates of grade 2 and 3 acute radiation esophagitis than the hypofractionated group.
10.Diagnostic value of prostate imaging reporting and data system version 1 and 2 in detection of prostate cancer in transition zone
Ximing WANG ; Jie BAO ; Mo ZHU ; Xiaoxia PING ; Chunhong HU ; Jianquan HOU ; Qilin XI ; Fenglin DONG ; Jun SUN ; Wenlu ZHAO ; Junkang SHEN
Chinese Journal of Radiology 2017;51(6):427-431
Objective To evaluate the diagnostic value of prostate imaging reporting and data system version 1 (PI-RADS V1) and version 2 (PI-RADS V2) for detection of prostate cancer (PCa) in the transition zone (TZ).Methods Seventy-seven patients with suspicious lesions in TZ on mpMRI were scored according to the PI-RADS system (V1 and V2) before MR-TRUS fusion guided biopsy prospectively.In all of the patients with suspicious tumors,respectively at least one lesion with a PI-RADS V1 assessment category of ≥3,was selected for biopsy.Independent sample t test was used to compare scores of PI-RADS V1 and V2 between PCa and benign prostatic hyperplasia (BPH).The diagnostic performance of PI-RADS V 1 and V2 for detection of PCa in the transition zone was compared by analyzing ROC basing on the results of MR-TRUS fusion guided biopsy.Results A cohort of 77 patients was performed including 31 cases of PCa (32 cores) and 46 cases of BPH (51 cores).PCa (V1:1 1.50±2.79;V2:4.28±0.99) had significantly higher scores of both PI-RADS V1 and PI-RADS V2 than BPH(V1:7.51± 1.63;V2∶2.61 ±0.67) (P<0.05).Using a PI-RADS V1 score cut-off ≥ 11,sensitivity and specificity in group PCa and BPH were calculated,which were 68.8%(22/32) and 96.1%(49/51) with a area under curve of 0.869;using a PI-RADS V2 score cut-off ≥4,which were 75.0% (24/32) and 90.2% (46/51) with a area under curve of 0.888,respectively.Conclusions PI-RADS system can indicate the likelihood of PCa of suspicious lesions in TZ on Mp-MRI.PI-RADS V2 perform better than V 1 for the assessment of prostate cancer in TZ.