Objective To investigate the primary target of injury resulting from cold preservation/reperfusion after liver transplantation in rats. Methods Male SD rats were divided into three groups randomly: sham group,UW 1-h group and UW 12-h group. Orthotopic liver transplantation was performed by using the technique described by Kamada with a modification. Survival rate of rats within 168 h postoperation was observed. Liver tissue specimens and blood samples were collected at 8 time points predetermined as 1,6,12,24,48,72,96 and 168 h postoperation. Six animals were used per time-point. The liver function was evaluated by serum ALT,AST and HA levels. Morphology was observed under light microscopy and TEM. Additionally,the incidence of apoptosis (AI) in hepatocytes and sinusoidal endothelial cells (SECs) were measured separately by TUNEL method.Results The survival rate at 168 h in UW 12-h group was 50 %,significantly lower than that in UW 1-h group (F= 6.39 ,P

Carcinoembryonic Antigen ; analysis ; Humans ; Luminescence

Objective To discuss the reasons of death in the in-patients with percutaneous coronary intervention(PCI).Methods Retrospective analysis was done in 33 cases who died in or after the procedures among 3 252 in-patients with PCI.Results 4 patients died in procedures and the others died after procedures.The major reasons of death in order were:cardiogenic shock,ventricular fibrillation,acute left heart failure,multiple organ failure,heart rupture and acute renal failure.The in-hospital mortality rate was 1.01%.Conclusion The major reasons of death of patients with PCI are cardiogenic shock,ventricular fibrillation and acute left heart failure.Taking some measures will reduce the in-hospital mortality rate.

Objective To study the value of CT perfusion in differential diagnosis of local recur and local radiofibrosis of nasopharyngeal carcinoma(NPC) at pre-and post-radiotherapy.Methods Dynamic CT scan was performed in 71 objectives: 14 in local recur group,22 in radiofibrosis group,15 in pre-radiotherapy NPC group and 20 in control group,the time-density curve(TDC) and the data of perfusion parameters were recorded and analyzed statistically.Results The TDC feature between local recur group,pre-radiotherapy NPC group and radiofibrosis group,control group had difference.The average of blood flow(BF) had significant differences by two by two comparison in four groups(P

Objective: To investigate the prevalence of intention to smoke and current smoking and identify its influencing factors among middle school students in Ningbo City, so as to provide insights into the development and implementation of tobacco control among adolescents. Methods: The epidemiological data of tobacco use among adolescents in Ningbo City in 2021 were collected, including demographics, tobacco use, parents' and close friends' smoking status, secondhand smoke exposure and tobacco advertisement exposure. The intention to smoke, current smoking, secondhand smoke exposure and tobacco advertisement exposure were descriptively analyzed among middle school students, and the factors affecting intention to smoke, current smoking were identified using a multivariable logistic regression analysis. Results: Totally 1 591 questionnaires were allocated to middle school students, and 1 574 valid questionnaires were recovered, with an effective recovery rate of 98.93%. The respondents included 787 junior high school students, 535 high school students and 252 vocational high school students, and the prevalence was intention to smoke and current smoking was 6.73% and 1.78% among the respondents, respectively. Multivariable logistic regression analysis identified boys (OR=2.398, 95%CI: 1.466-3.922), high school students (OR=2.051, 95%CI: 1.212-3.470), vocational high school students (OR=2.011, 95%CI: 1.108-3.650), more than 100 yuan disposable pocket money per week (OR=2.534, 95%CI: 1.301-4.935) and close friends' smoking (OR=4.310, 95% CI: 2.755-6.757) as risk factors of intention to smoke, and boys (OR=12.420, 95%CI: 1.645-93.753), close friends' smoking (OR=11.628, 95%CI: 4.348-31.250) and parents' smoking (OR=2.667, 95%CI: 1.029-6.897) as risk factors of current smoking among middle school students. The prevalence of secondhand smoke exposure and tobacco advertisement exposure was 76.43% and 51.65%, and television/video/cinema was the most likely route of tobacco advertisement exposure (42.06%). Conclusions The prevalence of intention to smoke and current smoking is low among middle school students in Ningbo City; however, the proportions of exposure to secondhand smoke and tobacco advertisement are relatively high. Tobacco control interventions are recommended to be intensified targeting high-risk populations, such as vocational high school students.

0.05).It could also decrease the times of wet manure induced by folia sennae,while it was of no effect on diarrhea induced by castor oil. CONCLUSION: Volatile oil from A.longiligulare T.L.Wu has anti-inflammation,analgesic and anti-diarrhea effect related to the cure for ulcerative colitis.

AIM: To investigate antioxidative and antinitrosative effects of volatile oil from A.longiligulare T.L.Wu on dextran sulfate sodium(DSS)-induced ulcerative colitis mice. METHODS: Balb/c mice were fed with 4% DSS solution for 7 d to induce ulcerative colitis.Using biochemical method,the activity of antioxidative enzyme SOD,MDA and NO were determined in normal,model,SASP and three mouse's groups with low,moderate and high volatile oil from A.longiligulare T.L.Wu respectively.At the same time,the activity of iNOS was also measured by immunohistopathology. RESULTS: The concentration of MDA and NO were reduced and SOD increased significantly in high-and moderate-volatile oil groups compared with those in the model group.The activity of iNOS was reduced significantly in high-and moderate-volatile oil groups compared with those in the model group.The results demonstrated that the expression of iNOS was significantly inhibited in DSSinduced ulcerative colitis mice after being treated with high or moderate-dosage volatile oil. CONCLUSION: Volatile oil from A.longiligulare T.L.Wu has antioxidative and antinitrosative effects which may be one of the mechanism for treating UC.

Objective: To investigate the chemopreventive effects of pioglitazone (exogenous PPAR? ligand) on rat colon aberrant crypt foci, a rat carcinogenesis model induced by dimethylhydrazine (DMH),and to compare pioglitazone with sulindac (a NSAID). Methods: Thirty two, 8 week old, female Sprague Dawley rats were randomly divided into four groups ( n =8 each). Group 1 rats were injected with DMH alone (120 mg?kg -1 , single subcutaneous injection). Group 2 rats were injected with saline alone. Group 3 rats were pre treated with sulindac (320 mg?kg -1 ) for 7 days before DMH initiation. Group 4 rats were treated with pioglitazone (100 mg?kg -1 ). The animals were killed at the end of the experiment (week 5) and the colons were stained with methylene blue. The aberrant crypt foci (ACF) of the colonic mucosa were assessed. Results: In Group 1 rats (DMH only), the average numbers of ACF/colon and AC/colon were (182?93) and (263?198), respectively. In Group 2 (saline group) rats, no ACF were found. In Group 3 (sulindac group) rats, the average numbers of ACF/colon and AC/colon were (91?49) and (140?69), respectively. Both of them were decreased significantly compared with the values in Group 1 ( P

AIM: To study the protective effects of dexamethasone agains t ototoxicity of aminoglycoside antibiotics in guinea pigs. METHODS: Guinea pigs were divided into five groups. Group One: gentamycin, im; Group Two: amikacin, im; Group Three: gentamycin+ dexamethasone, im; Group Four: amika cin+ dexamethasone,im; Group Five: NS, im, all for two weeks. After three weeks, all animals were examined including tympanic mucous membrane reaction, nystagmu s depression rates and cochleae morphology. RESULTS: In Group Th ree and Four, the changes of morphology were slighter than those in Group One an d Two. CONCLUSION: Dexamethasone can weaken ototoxicity of amino glycoside antibiotics.

Objective To investigate the effects of cold preservation and reperfusion injury on the regen-eration of donor liver and to study the mechanisms. Methods Male SD rats were divided in to sham group (6 rats), UW 1 h group (48 rats) and UW 12 h group (48 rats). Liver tissue specimens were collected at different time points after orthotopic liver transplantation or sham operation. The morphology of liver tissue was observed via light microscope and transmission electron microscope. Proliferation of hepatocytes and sinusoidal endothelial cells (SECs) were assessed by a double immunostaining technique using antibodies against rat endothelial cell antigen-1 and bromodeoxyuridine (BrdU). Expression of vascular endothelial growth factor (VEGF) and its receptors, fins-like tyrosine kinase-1 (flt-1) and fetal liver kinase-1 (flk-1) was evaluated by immunohistochemistry. The mRNA expression of flt-1 was detected by a RT-PCR method. Mean comparison in groups was conducted by one-way ANOVA or t test. Results BrdU labeling indexes of hepatocytes and SECs in UW 12 h group was significantly higher than those in UW 1 h group (F = 61.45,41.4, P < 0.05). The proliferation of hepatocytes peaked at 48 h after operation in both UW 1 h group and UW 12 h group. However, the proliferation of SECs was fallen behind compared to hepatocytes, with a peak appeared at 72 h in UW 1 h group and at 96 h in UW 12 h group, respec-tively. The expression of VEGF was up-regulated in both UW 1 h group and UW 12 h group compared to sham group. Furthermore, expression of flt-1 and flk-1 was found to be mainly limited in SECs, with a peak in expres-sion occurring between 72 h and 96 h, coinciding with the peak in SECs proliferation in UW 1 h group. Conversely, flt-1 was found to be reduced significantly on mRNA level at any time throughout the experiment in UW 12 h group compared to sham group (F = 141.67, P < 0.05). Conclusion Reduced expression of flt-1 results in a retarded regeneration of SECs, and then the recovery of rat donor liver function is delayed after cold-preserved transplantation.