7 layers).Mito tic figures are infrequent and usually limited to the basal layer.C onclusionsPUNLMP is not associated with invasion or metastases and should be treated locally.However,long term clinical follow-up is warranted.

A study was made; of the effect of malotilate on the acute liver injury induced by carbon tetrachlorid,e ( CC14 ) and d-galactosamine in mice. Malotilate ( 50~150mg/kg ig?3 ) significantly inhibited the elevation of serum glu tamic pyruvic transaminase ( SGPT ) in CC14- intoxicated mice.At the dose of 100mg/kg ig?3, malotilate remarkably increased the content of hepatic glycogea in CCl4-injected mice. The contents of serum protein, liver protein, and cytochrom P-450 in liver hemogenate were increased by malotilate ( 100mg/kg ig?3) in CC14-intoxicated mice. The drug also reduced the accumulation of liver triglycerides induced by CCl4 in mice.In addition to, malotilate(50mg /kg, ip?5) could act against the increase of SGPT and the decrease of liver protein content induced with d-galactosamine in mice. These results suggest that malotilate may be a new therapeutic agent for liver injury.

The effectiveness of regional health information platform was judged by overall assessment of its data contents, data quality, data safety and beneficial results in order to promote the mutual link and mutual communi-cation between the platforms and data centers at the provincial, municipal and county levels, speed up the data sharing and professional work cooperation, and meet the different information needs of different users.

Objective:To improve equipment intact rate and utilization rate, make the hospital better social and economic benefits. Methods:Through the cultivation of high-tech talent, the use of high-tech means, the use of high-tech repair tools, repair and maintenance of science, strengthening external relations, making full use of social resources. Results:Construction of a high level of equipment repair team gradually, make full use of computer and network, the use of new tools to improve the quality of repair, repair. Scientific maintenance and repair, make full use of social resources, ensure medical equipment intact rate. Conclusion:Improve the medical equipment intact rate;give full play to the comprehensive benefits of modern medical equipment, to create greater social and economic benefits for the hospital.

To meet the requirement of cultivating talented people,the following problems were explored in the medical biochemistry experiments classes including teaching content,teaching format,teaching management,and evaluation of the teaching performance and strengthening of students' capacity cultivation.

Microteachingisthescientifictrainingsystemtoimprovetheteachingskills.Therea-sonable application of microteaching in training of the clinic teaching may effectively boost their clinic teaching techniques and dramatically promote the overall construction of medical college teachers.

Objective:To compare the content of polydatin and emodin between polygonum cuspidatum pieces and dispensing gran-ules to provide theoretical basis for the improvement of preparation process of the granules. Methods:An HPLC method with linear gra-dient elution was used to determine the content of polydatin and emodin in the pieces and dispensing granules, and the difference in the two contents was compared. Results: The content of polydatin and emodin in polygonum cuspidatum pieces met the requirement de-scribed in China pharmacopoeia, which was lower than that in the dispensing granules, and the contents of the two components in the commercial dispensing granules were not equivalent to the labeled amount, which was 1/15 and 1/27 of that in the pieces. Conclu-sion:The transport rate of the traditional preparation process of dispensing granules is with poor efficiency, which should be improved.

Phage display is a widely used gene engineering high technology.Through the display of exogenous peptides or proteins fused specific coat protein on the surface of phages,it is possible to construct proteins or peptides libraries and screen interesting proteins,peptides and antibodies successfully.Most commonly used phage display technology is phagemid/helper phage system,in which helper phages are essential for the replication and assembly of phagemid particles.In this review,in combination with the newest research dynamic status,we summarize phagemid/helper phage double-genome system.We mainly emphasized the features and mutation mechanisms of different helper phages.We also made some prospects for the future directions,in the meanwhile,we also expect that our experience can provide some help for further maturity of the technology.

OBJECTIVE: To study the genotype distribution of cytochrome CYP3A4, CYP2C9, CYP2C19, CYP2D6 in Han and Mongolian Chinese population and the allele and genotype frequency are compared between the Han and Mongolian. METHODS: CYP3A4, CYP2C9, CYP2C19, CYP2D6 of Han and Mongolian Chinese population were genotyped by PCR-RFLP. RESULTS: The allele frequencies of CYP3A4 * 5 in Han and Mongolian are 0, the allele frequencies of CYP3A4 * 18 in Han and Mongolian are 0.1838, 0.2025. The allele frequencies of CYP2C9 * 2 in Han and Mongolian are 0.0110, 0.0253; the allele frequencies of CYP2C9 * 13 in Han and Mongolian are 0, 0.003 2.The allele frequencies of CYP2C19 * 2 in Han and Mongolian are 0.386 0, 0.4146, the allele frequencies of CYP2C19 * 3 are 0.0515, 0.0443. The allele frequencies of CYP2D6 * 10 in Han and Mongolian are 0.5735, 0.4652. CONCLUSION: The study shows that there are no significant ethnic differences in the distribution of CYP3A4 * 18, CYP2C19 * 2, CYP2C19 * 3, CYP2D6 * 10 genotypes in Han and Mongolian. The CYP3A4 * 5 genotype is not found in this study and only one CYP2C9 * 1/* 13 genotype is found in Mongolian. For CYP2C9 * 2, the Mongolian is significantly lower than the Han, (P=0.023).

Objective To investigate the clinical features , treatment regimen , and prognosis evaluation of tertiary peritonitis (TP). Methods Seventy-eight cases with TP were randomly enrolled into 2 groups, including the simple western medicine-treated group (32 cases) and the integrated traditional Chinese and western medicine-treated group (46 cases). The prognoses were evaluated according to the acute physiology and chronic health evaluationⅢ (APACHEⅢ, APⅢ) scoring. Results The mortality rate was 71.9% (23 of 32) in patients received the simple western medicine and was 32.6%(15 of 46) in patients received the integrated traditional Chinese and western medicine with significant difference between these two groups (P < 0.01). There was a significant correlation between AP Ⅲscore and actual mortality (r=0.73,P<0.01), and predicted mortality (r=0.76, P<0.01). Conclusions The therapeutic effect is acceptable and satisfactory for the TP patients received the integrated traditional Chinese and western medicine. The AP Ⅲ scoring system can be used to predict the prognosis of TP patients.