No abstract available.
Animals ; Dermatitis, Contact* ; Dinitrochlorobenzene* ; Guinea Pigs* ; Guinea* ; Spleen*

No abstract available.
Cleft Lip* ; Congenital Abnormalities* ; Skin*

BACKGROUND: bFGF, a member of the fibroblast growth factor family, potently induces vascular smooth muscle cell proliferation and decreased synthesis of the collagens. OBJECTIVE: For further investigation of the effect of bFGF on extracellular matrix homeostasis in the skin, we evaluated the expression of type I and type VII collagen gene at the transcriptional levels. METHOD: We examined that recombinant human bFGF affects the expression of genes involved in ECM synthesis and remodeling in human dermal fibroblasts cultures as judged by Northern blot analysis. RESULTS: The steady state levels of type I and VII collagen gene mRNA were decreased with age dependent pattern up to 0.13 and 0.44 folds respectively. The transcriptional levels of type I collagen mRNA were increased by TGF-B, treatment but markedly decreased by bFGF as well as TNF-a. But there were no synergistic effects bFGF and TNF-a on type I collagen gene expression. The levels of type VII collagen gene expression were increased by both bFGF and TGF-B,. The TNF-a showed slightly antagnostic effects on type VII collagen gene expression. CONCLUSION: The type I and VII collagen gene expression in dermal fibroblasts is clearly subjected to modulation by the cytokines including bFGF with uncoordinate regulatory pathway. In addition to its function of vascular proliferation, bFGF also may play a major role in physiologic skin condition and in repair process such as formation of a stable dermoepidermal junction during skin wound healing.
Blotting, Northern ; Cell Proliferation ; Collagen Type I ; Collagen Type VII ; Collagen* ; Cytokines ; Extracellular Matrix ; Fibroblast Growth Factors ; Fibroblasts* ; Gene Expression* ; Homeostasis ; Humans ; Methods ; Muscle, Smooth, Vascular ; RNA, Messenger ; Skin ; Wound Healing

No abstract available.

Hamartoma of the breast is relatively rare benign tumor, which is a well-circumscribed mass mainly composed of fibrous stroma, a(tipose tissue, ducts and acini. Muscular hamartoma of the breast is mainly composed of smooth muscle, and is extremely rare because proper smooth muscle is normaly absent in the breast except in the nipple. We describe a rare case of muscular hamartoma of the breast in a 38-year-old woman. This tumor was located in the upper outer quadrant and a 3 x 2.5 x 2 cm sized, well-demarcated but not encapsulated mass, The mass consisted mainly of irregularly arranged smooth muscle bundles in the fibrous stroma with lobular units and admixed fat cells. The origin of smooth muscle in hamartoma is not well known.
Female ; Humans ; Hamartoma

No abstract available.
Intestinal Obstruction* ; Mucocutaneous Lymph Node Syndrome*

The authors developed a comfortable program for routine work of surgical pathology. We used IBM PC (80386) and Foxbase plus program. The main function of this program was automatic coding and concurrent surgical report printing. During gross printing, previous biopsy number and its diagnosis were automatically searched and printed below gross description. The reported data were stored during surgical report printing simultaneously, and thus the typist's workload became considerably reduced. Search for specific cases could be performed by patient's name, surgical number, hospital number, diagnostic code numbers (SNOMED code micro glossary), and certain disease entities on very short duration.
Biopsy ; Clinical Coding ; Diagnosis ; Pathology, Surgical*

Sclerosing hemangioma of the lung is uncommon benign neoplasm of uncertain histogenesis. Their radiologic appearance is relatively distinct and well defined. Recently we experienced 3 cases of sclerosing hemangioma of the lung in 54, 52, 51 years old women. The light microscopic findings are similar to the features reported by Liebow and Hubbell (1956).
Female ; Histiocytoma, Benign Fibrous* ; Humans ; Lung* ; Pulmonary Sclerosing Hemangioma

A 32-year-old housewife who resides in Taegu was admitted in Yeungman University Hospital due to right upper quadrant abdminal pain of 2 mounths'duration. An abdomical CT and ultrasonography revealed a relatively well demarcated low density mass in the right lobe of liver. Right hepatic lobectomy was performed on the clinical impression of hepatoma. On the light microscopic study, the lobulated liver mass showed extensive central necrosis and fibrosis, with large numbers of pseudotubercles therein. The pseudotubercles have distorted helminthic eggs frequently. The submitted common bile duct lymph node also showed a few pseudotubercles. The eggs recovered from the tissue homogenate measured 140~152 micrometer by 75~85 micrometer in size and were unembryonated and light yellow to brown. The eggs were determined as those of Fasciola species. We reported the present case as 11th one of human fascioliasis in korea.
Humans

The aim of this study was to investigate the effect of hypoxia-reoxygenation on the proliferation of fibroblast, and to elucidate the role of oxygen free radicals in this process. Malme-3 fibroblast, derived from human skin fibroblast, was used for this study. The hypoxia or reoxygenation condition was made by exposing cultured cells to the environment of 95% N2, 5% CO2 or 95% room air, 5% CO2, respectively. Cell proliferation was estimated by the cell numbed, and DNA synthesis was measured by the [3H]-thymidine uptake. Release of oxygen free radicals was measured by the means of Ohkawa's method of lipid peroxidation. The effect of oxygen free radicals was confirmed by using dimethylthiourea(DMTU) and alpha-tocopherol, two known oxygen free radical scavengers. The results are as follows: 1. The dissolved oxygen of the culture medium was 8.97+/-1.23 ppm in the normal condition. When the culture dish was exposed to the hypoxic condition for 3 or 6 hours, the dissolved oxygen of the culture medium decreased markedly to the level of 3.10+/-0.46 ppm or 2.37+/-0.47 ppm, respectively 2. The number of cultured cells increased in a hypoxia duration-dependent manner up to 6 hours when the cells were cultured for 24 hours after hypoxia. The same pattern was observed in the cells cultured for 48 hours after hypoxia. Lipid peroxidation in the culture increased after the exposure to hypoxia-reoxygenation. DMTU or alpha-tocopherol blocked the increase in lipid peroxidation induced by the exposure to hypoxia-reoxygenation. 3. [3H]-thymidine uptake of the cultured cells increased after the exposure to hypoxia-reoxygenation. 4. DMTU or alpha-tocopherol blocked the proliferation of fibroblasts induced by the exposure to hypoxia-reoxygenation. The increase in lactate dehydrogenase (LDH) activity was also noted after the exposure to hypoxia-reoxygenation, and this increase was blocked by DMTU or alpha-tocopherol. These results indicate that the hypoxia-reoxygenation induces the proliferation of fibroblasts, and that oxygen free radicals play an important role in this process. Moreover, oxygen free radical scavengers may be of potential therapeutic value in preventing fibrosis.
alpha-Tocopherol ; Anoxia ; Cell Line* ; Cell Proliferation ; Cells, Cultured ; DNA ; Fibroblasts* ; Fibrosis ; Free Radical Scavengers* ; Free Radicals ; Humans* ; L-Lactate Dehydrogenase ; Lipid Peroxidation ; Oxygen* ; Skin