The immune response to M. tuberculosis engages T-cell medicated reactions which determine the degree of resistance and also the clinical pattern of disease. Although antibodies produced by the infected host have yet no proven protection, they are appilicable for alternative diagnostic tests in tuberculosis. Preparation of purified antigens from Mycobacteria with specific antigenic determinants would improve serological diagnosis in tuberculosis. The antigen was prepared by extraction of M. tuberculosis with perchloric acid which was found to be major bands of 71, 42, 38 and 10 kD by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thirteen sera from patients with active tuberculosis showed positive reactions to TB-P by ELISA, but the reaction to perchloric acid extract antigen is weaker than to PPD. The IgG subclass profiles to TB-P were IgG1, IgG2 and IgG4. By means of western blotting, the antibodies in the tuberculous patients showed the reaction with antigens of lower molecular weight such as 14, 12, 11 and 10 kD. These results suggested that the perchloric acid soluble antigen of M. tuberculosis might be more related with cell-mediated immune reactions rather than humoral immune reactions.
Antibodies ; Blotting, Western ; Diagnosis ; Diagnostic Tests, Routine ; Electrophoresis ; Enzyme-Linked Immunosorbent Assay ; Epitopes ; Humans ; Immunoglobulin G ; Molecular Weight ; Sodium ; T-Lymphocytes ; Tuberculosis*

This study was designed to reveal the correlationship between the internal derangement state of TMJ and clinical characteristics including pain and mandibular dusfuntion. One hundred and twenty five subjects with TMJ signs and symptoms were chosen for two years. The level of pain and mandibular dysfuntion were evaluated by Visual Analog Scale(VAS) and Craniomandibular Index(CMI). The diagnostic categories of TMJ internal derangement were determined by arthrography and they included normal disc position, anterior disc displacement with reduction(ADDR), rotational disc displacement with reduction(RDDR), andterior disc displacement without reduction(ADDNR), and rotational disc displacement without reduction(RDDNR). Also disc perforation was used as a criteria to divide the diagnostic subgroups. The obtained results were as follows ; 1. The patient distribution of each group was 5 in normal disc position(4%), 40 in ADDR(32%), 30 in RDDR(24%), 34 in ADDNR(27%), and 16 in RDDNR(13%). 2. Perforation was observed in 8% of ADDR, 10% of RDDR, 32% of ADDNR, and 19% of RDDNR. 3. CMI of perforation group was higher than that of reduction or normal group(P<0.005), but vas showed no significant difference. 4. CMI of non-reduction group was higher than that of non-perforation group in reduction group(P<0.05). 5. There were no significant differences of CMI and VAS between anterior disc displacement group and rotational disc displacement group in both reduction and non-reduction group. 6. CMI of RDDNR group was higher than that of RDDR group(P<0.05). 7. There were no significant difference of CMI and VAS between bilateral involvement group and unilateral involvement group(p>0.05).
Arthrography ; Humans ; Temporomandibular Joint*

The author evaluated the effects of taxol, a microtubular inhibitor, as a possible radiation sensitizer and the production of prostaglandins on three human cancer cell lines(KB, RPMI-2650 and SW-13) and one murine cell line(L929). Each cell line was divided into four groups(control, taxol only, radiation only and combination of taxol and radiation). The treatment consisted of a single irradiation of 10 Gy and graded doses(5, 50, 100, 200, 300, 500 nM) of taxol for a 24-h period. The cytotoxicity of taxol alone was measured at 1 day after(1-day group) and 4 days after(4-day group) the treatment. The survival ratio of cell was analyzed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyl tetrazolium bromide) test. Prostaglandins(PGE2 and PGI2) were measured in the culture medium by a radioimmunoassay. The results obtained were as follows ; 1. There was a significantly in creased cytotoxicity of KB cells in 4-day group than those in 1-day group. Therr was a high correlation between doses of taxol and cell viability in both groups(1-day group R=0.82741, 4-day group R=0.84655). 2. There was a significantly increased cytotoxicity of RPMI-2650 cells treated with high concentration of taxol in 4-day group than those in 1-day group. Also there was a high correlation between doses of taxol and cell viability in 4-day group(R=0.93917). 3. There was a significantly increased cytotoxicity of SW-13 cell treated with high concentration of taxol in 4-day group than those in 1-day group. However no high correlation was observed between doses of taxol and cell viability in both groups(1-day group R=0.46362, 4-day group R=0.65425). 4. There was a significantly increased cytotoxicity of L929 cells treated with low concentration of taxol in 4-day group than those in 1-day group. At the same time, there was a low correlation between doses of taxol and cell viability in both groups(1-day group R=0.34237, 4-day group R=0.23381). 5. In 1-day group of L929 cells, higher cytotoxicities were observed in the groups treated with 500 nM taxil than given 10 Gy radiation alone showed a radiosensitizing effect by taxol. 6. In addition to L929 cells, all cancer cells treated with a commbinationof taxol and radiation in 4-day group appeared ti have some fragmented nuclei and to float on the medium. In addition, L929 cells appeared to be more confluent. 7. The level of PGE2 production was the highest in the contol KB cells. This appeared to increase in every experimental group of all three cancer except L929 cells. There was a significantly increased production of PGE2 in SW-13 cells treated with a combination taxol and radiation compared to the other experimental groups. 8. The level of PGI2 production in the contol group RPMI-2650 cells was the highest. This appeared to increase in every experimental group of all cells except in SW-13 cells. This also increased signigicantly in RPMI-2650 cells treated with a cimbination of taxol and radiation compared to the other experimental groups.
Cell Line ; Cell Survival ; Dinoprostone ; Epoprostenol ; Humans ; KB Cells ; Paclitaxel* ; Prostaglandins ; Radiation Dosage ; Radiation, Ionizing* ; Radiation-Sensitizing Agents ; Radioimmunoassay

We have performed the prick test on 70 patients with atopic lermatitis and at the same time measured the levels of serum IgE and peripheral eosinophil counts. The following results were obtained: 1. Patients with atopic dermatitis showed higher rates of positivty in the prick tests, espe cially to inhalant, allergens. 2. The levels of IgE were increased in these patients and those who were positive to at least one allergen in the prick tests showed higher levels of IgE ha those who were negative in the prick tests. 3. The patients with atopic dermatitis with a past history of allergic rhinitis demonstrated higher rates of skin test positivity. 4. There was no correlation between the levels of serum IgE and the peripheral eosinophil counts in these patients with atopic dermatitis,
Allergens ; Dermatitis, Atopic* ; Eosinophils ; Humans ; Immunoglobulin E* ; Rhinitis ; Skin Tests

Radiation sensitivity data was generated for two human cancer cell lines(KB, RPMI 2650) and human primary gingival fibroblast was tested three times using a viable cell number counting with a hemocytometer, MTT(3-[4,5-dimethylthiazol 2-yl]-2,5-dipheny tetrazolium bromide) assay, and LDH(Lactate dehydrogenase) assay. Single irradiation of 2, 4, 6, 10, 15, 20 Gy were aplied to the tumor cell lines and the primary cultured gingical fibroblast. The two fractions of 4 Gy an d 10 Gy were seperated with a 4 hour time interval. The irradiation was done with 241.5 cGy/min dose rate using 137 Cs MK cell irradiator at room temperature. The obtained results were as followed: 1. There was significantly different viable cell numbers as the amount of radiation dose on the tested cells were cell number counted with a hemocytometer, In fractions, there were more viable cells remaining, 2. Phase-contrast microscopically, radiation-induced morphologic changes were pronounced on the tumor cells, however, a lmost no differences on the gingival fibroblast. 3. There was significantly different absorbance at 2 Gy on RPMI 2650, 4 Gy on KB and GF in MTT assay. In fractions, the absorbance was significantly higher on KB. 4. THe level of extracellular LDH activity in the experimental group was significantly higher in the 2-4 Gy than the co ntrol group. 5. The total level of extracellular and intracellular LDH activity was decreased as increased amounts of radiation dose was applied.
Cell Count ; Cell Line, Tumor* ; Fibroblasts ; Humans ; Radiation Tolerance*

No abstract available.
Dapsone* ; Dermatitis* ; Neutrophils*

No abstract available.
Hyperlipidemias ; Skin Diseases

In order to examine elderly depression in the elderly that too weakened cognitively, and physically to check depressive symptoms reliably, the authors deviced collateral source version of elderly depression scale and tested it's validity. Sixty elderly depression patients and forty-eight old person in normal group completed the KGDS (Korean Form of Geriatric Depression Scale), and their collateral source (CS) completed a CS version of the KGDS (CS-KGDS). The differences of CS-KGDS means was signified at alpha=.001 level (patients group mean=18.33 (4.71), control group mean=7.60 (5.25), t=11.18). The values of Cronbach's alpha and Split-half reliability were .88 and .81 respectively, and correlational coefficent with KGDS was .68. On factor analysis, 5 factors were extracted. They were labeled 'Physical weakening and decreased vitality' (factor 1), 'Emotional discomfort' (factor 2), 'Negative thinking and Unhappiness feeling' (factor 3), 'Cognitive dysfunction' (factor 4), 'Decreased social interest and activity' (factor 5), which congruous with internal structure of KGDS. The result of discrimination analysis showed 87.09% of hit ratio, and suggested the score of 13 as optimal cut-off score. This cut-off score was identical to that of KGDS. Conclusively, CS-KGDS could be a useful tool for evaluaion of elderly depression instead of KGDS in such case that KGDS can not be administered reliably.
Aged ; Depression* ; Discrimination (Psychology) ; Humans ; Thinking

PURPOSE: This study was to investigate the age and sex differences of the fractal dimension of the normal parotid glands in the digitized CT images. MATERIALS AND METHODS: The six groups, which were composed of 42 men and women from 20`s, 40`s and 60`s and over were picked. Each group contained seven people of the same sex. The normal parotid CT images were digitized, and their fractal dimensions were calculated using Scion ImagePC program. RESULTS: The mean of fractal dimensions in males was 1.7292(+/-0.0588) and 1.6329(+/-0.0425) in females. The mean of fractal dimensions in young males was 1.7617, 1.7328 in middle males, and 1.6933 in old males. The mean of fractal dimensions in young females was 1.6318, 1.6365 in middle females, and 1.6303 in old females. There was no statistical difference in fractal dimension between left and right parotid gland of the same subject(p>0.05). Fractal dimensions in male were decreased in older group(p<0.05) and larger than female in same age group(p<0.05). But fractal dimensions in female had a tendency to decrease in older group but no statistical difference between ages(p>0.05). CONCLUSIONS: The fractal dimension of parotid glands in the digitized CT images will be useful to evaluate the age and sex differences.
Female ; Fractals* ; Humans ; Male ; Parotid Gland* ; Sex Characteristics

No abstract available.
Aneurysm* ; Arteries* ; Cardiopulmonary Bypass* ; Circulatory Arrest, Deep Hypothermia Induced*