Many carcinomas have an active mononuclear cell infiltrates surrounding tumor. Various in vitro assays have shown that cellular constituents of peripheral blood mononuclear cells(PBMC) can alter growth of carcinoma cell line. Author compared the effects of normal fibroblasts on squamous cell carcinoma cell line(SCL-1) along with those of sctivated and/or nonactivated PBMC on SCI 1 using a skin equivalent system. This system prevents direct cellular contact by growing SCL-1 on an overlying Millicell-HA membrane and normal fibroblast or supernatants of PBMC in a lower chamber. Normal fibroblasts enhanced the outgrowth of SCL-1 and induced a more organized phenotype of SCL-1. Supernatants from nonstimulated PBMC suppressed outgrowth of SCL 1, and concanavalin A stimulated PBMC supernatants alterd rnorphology of cultured SCL-1 from a disorganized phenotype to a more organized phenotype. It is concluded that fibroblasts and PBMC may affect the growth and differentiation of SCL-1 via their mediators(cytokines)
Carcinoma, Squamous Cell* ; Cell Line* ; Concanavalin A ; Fibroblasts* ; Membranes ; Phenotype ; Skin

No abstract available.
Constriction, Pathologic*

No abstract available.
Fournier Gangrene*

No abstract available.
Extremities*

Hypothalamic hamartomas are often associated with early onset gelastic seizures, precocious puberty, behavioral problem and suboptimal response to antiepiletptic drugs. Until now, four surgical options have been reported to reduce seizure by >50%. Surgical excision have good seizure outcome but postoperative complications were not infrequent, whether by pterional or transcallosal interforniceal approach. Radiosurgery is noninvasive alternative to resective surgery but the effect usually does not appear until several months later. Radiofrequency ablation is less invasive than surgical resection and its effect is immediate, but lacks long term follow-up data. It also requires three dimensional analysis of the lesion to enhance effi cacy and safety. As hypothalamic hamartoma is intrinsically epileptogenic and epileptogenic discharges spread from the lesion, blocking the seizure propagation through endoscopic disconnection is regarded as an effective and safer option. Surgical choice for a particular patient should take into account the hamartoma’s size, location, surgeon’s preferences, possible complication as well as the effect and risk of the various surgical methods. In the present review, open surgery, endoscopic disconnection, radiosurgery and radiofrequency ablation are discussed.

No abstract available.
Diagnosis* ; Heart Failure* ; Heart Septal Defects, Ventricular ; Heart*

No abstract available.
Lymph Nodes* ; Uterine Cervical Neoplasms*

No abstract available.
Blood Platelets* ; Humans ; Platelet Aggregation* ; Plateletpheresis* ; Tissue Donors*

PURPOSE: Bile acids (especially deoxycholate) was known to be toxic and mutagenic on colon epithelium. They proposed at least four mechanisms for the bile acid toxicity. It is the one of these mechanisms that bile acid inhibits the xenobiotic metabolizing enzyme activity (esp glutathione S-transferase, GST). So we measured the cytosolic GST level of colon carcinoma cell lines after deoxycholate exposure whether or not the deoxycholate lowered the cytosolic GST activity. METHODS: Three colon cancer cell lines (LoVo, SW480, HT29) were used for this study. We calculated the cellular toxicity by MTS method. And cytosolic GST activity was measured according to the method as Habig described. For total GST activity, 2.5 mM 1-chloro-2,4-dinitrobenzene was used for substrate, and measured as absorbance in 340 nm. RESULTS: Basal cytosolic GST level for LoVo, SW480, HT29 cell line was 514.59+/-27.01, 291.63+/-38.44 and 344.58+/-47.92 nmol/min/mg cytosol protein. GST level did not changed significantly after 5 days culture without DCA. But GST level was decreased significantly to 128.63+/-21.35, 134.33+/-41.76 and 163.10+/-22.73 nmol/min/mg cytosol protein each cell line after 5 days deoxycholate exposure (p<0.005). CONCLUSION: Cytosolic GST level was lowered significantly after deoxycholate exposure for 5 days. One of the mechanisms of bile acid toxicity for colon cancer cell is proposed to inhibit cytosolic GST activity.
Bile ; Bile Acids and Salts ; Cell Line* ; Colon* ; Colonic Neoplasms* ; Cytosol* ; Deoxycholic Acid* ; Dinitrochlorobenzene ; Epithelium ; Glutathione Transferase* ; Glutathione* ; HT29 Cells ; Humans

No abstract available.
Cervix Uteri* ; Drug Therapy* ; Female