OBJECTIVE: This study was performed to evaluate the usefulness of New cervicography by comparing cervicographic finding with Papanicolaou smear and result of colposcopically directed biopsy. METHODS: This study group consisted of 189 patients who visited the Department of Obstetrics and Gynecology, Chung-Ang University Pildong Hospital from September 1998 to August 1999. All women simultaneously underwent Papanicolaou smear and New cervicography. If either method was positive, the patient was referred for colposcopically directed biopsy. RESULTS: The New cervicography was significantly more sensitive than the Papanicolaou smear(92.2% vs 60.8%, p<0.01), whereas the Papanicolaou smear was significantly more specific than the New cervicography(99.3% vs 75.0%, p<0.05). The false positive rate of New cervicography was 75%, significantly higher than 0.7% of Papanicolaou smear. There was no significant difference between the negative predictive value of cervicography and Papanicolaou smear. When New cervicography and Papanicolaou smear were used together, the sensitivity was significantly higher than Papanicolaou smear used alone(98.1% vs 60.8%, p<0.01) and false negative rate was significantly lower than Papanicolaou smear(1.9% vs 39.2%, p<0.01). However the specificity, positive predictive value of the combined test were lower than Papanicolaou smear. CONCLUSION: Cervicography is one of the Papanicolaou smear adjunctive tests and a useful method to detect cervical cancer. Our study confirms the previously suspected low sensitivity of Papanicolaou smear and demonstrate that cervicography is more sensitive than Papanicolaou smear and less specific. From the above results, when New cervicography and Papanicolaou smear were used together, the detection rate of cervical cancer will be increased more easily since the cases missed by one method may be picked up by another.
Biopsy ; Female ; Gynecology ; Humans ; Mass Screening* ; Obstetrics ; Papanicolaou Test ; Sensitivity and Specificity ; Uterine Cervical Neoplasms*

A boy (Hellman dental age III B) with mandibular prognathism was treated by means of the labiolingual appliance and the chin cap. Following results were obtained; 1) Except the problem of development, most of treatment objectives were achieved after 8 months of active treatment. 2) The axial inclinations of upper and lower incisors were changed by the treatment. 3) The most notable change was the reduction of genial angle. 4) The patient has retained the good occlusal stability till after 18 months of retention.
Chin ; Humans ; Incisor ; Male ; Prognathism*

No abstract available.
Hip* ; Natural History* ; Technetium Tc 99m Medronate*

PURPOSE: In adriamycin(ADR)-induced cardiomyopathy, several different mechanisms are suggested. However, little information is available regarding the role of apoptosis. In the present study, we examined the induction of apoptosis on ADR treatment and anti-apoptotic effects of probucol, a lipid-lowering drug, and we also studied the changes of bcl-2 expression in order to see the molecular mechanisms underlying the effect of probucol. METHODS: Cardiac myocytes were isolated from 3-day-old rats, and cultured in low(1 pM) or high doses(10pM) of ADR for 24 hours. Probucol(50 pM) was added 30 minutes before ADR administration. Apoptosis was determined by TUNEL staining, and bcl-2 expression was estimated by immunocytochemistry. RESULTS: The number of TUNEL-positive cells significantly increased in both groups treated with ADR. However, anti-apoptotic effect of probucol was evident only in low dose. In addition, the expression of bcl-2 was significantly increased only in the low-dose ADR treatment group and its expression was inhibited by pretreatment of probucol. CONCLUSION: These results suggest that apoptosis might play an important role in ADR-induced cardiotoxicity, and ADR-induced apoptosis was partially prevented by pretreatment of probucol. And ADR-induced apoptosis was not related with depression of bcl-2. Additionally, inhibition of bcl-2 gene expression of low-dose ADR treatment group by probucol suggests that another cell survival mechanism could be implicated in the action of probucol. (J Korean Pediatr Soc 2000;43:746-754)
Animals ; Apoptosis* ; Cardiomyopathies ; Cell Survival ; Depression ; Doxorubicin ; Genes, bcl-2 ; Immunohistochemistry ; In Situ Nick-End Labeling ; Myocytes, Cardiac* ; Probucol* ; Rats*

Cartilage ; Cleft Lip ; Congenital Abnormalities ; Ear ; Humans ; Palate ; Rhinoplasty ; Transplants ; Young Adult

BACKGROUND: IL-4 is known as a potent stimulating factor and, TNF-α and IFN-γ suppress collagen synthesis of dermal fibroblasts. However, relatively little is known about interaction of these cytokines. OBJECTIVE: The purpose of this study was to evaluate the interactional effect of TNF-α and INF-r for the type I collagen gene expression and promoter activation produced by IL-4. METHODS: Using dermal fibroblasts from the normal skin cultured with three cytokines, IL-4, TNF-α and IFN-γ, we examined Northern blot analysis with each cDNA and assayed CAT activity with human pro a 2(l)collagen(COL1A2)/CAT reporter gene chimeric construct. RESULTS: Compared to the control, treating with IL-4 resulted in prominent elevation of both type I procollagen mRNA levels and prce 2(I) collagen promoter activity. IL-4 with TNF-α suppressed the IL-4 induced elevations, whereas IL-4 with IFN-γ did not reveal the obvious suppression of the elevations produced by IL-4. Fibroolasts treated with IL-4 together with IFN-γ and TNF-α completely abolished the type I procollagen gene expression and the activation of COL1A2 promoter gene elicited by IL-4. CONCLUSION: IL-4 induced enhancement of type I collagen gene expression was synergistically suppressed by TNF-α and IFN-γ on transcriptional level.
Animals ; Blotting, Northern ; Cats ; Collagen ; Collagen Type I ; Cytokines ; DNA, Complementary ; Fibroblasts* ; Gene Expression ; Genes, Reporter ; Humans* ; Interleukin-4* ; Necrosis* ; RNA, Messenger ; Skin*

No abstract available.
Constriction, Pathologic*

Many carcinomas have an active mononuclear cell infiltrates surrounding tumor. Various in vitro assays have shown that cellular constituents of peripheral blood mononuclear cells(PBMC) can alter growth of carcinoma cell line. Author compared the effects of normal fibroblasts on squamous cell carcinoma cell line(SCL-1) along with those of sctivated and/or nonactivated PBMC on SCI 1 using a skin equivalent system. This system prevents direct cellular contact by growing SCL-1 on an overlying Millicell-HA membrane and normal fibroblast or supernatants of PBMC in a lower chamber. Normal fibroblasts enhanced the outgrowth of SCL-1 and induced a more organized phenotype of SCL-1. Supernatants from nonstimulated PBMC suppressed outgrowth of SCL 1, and concanavalin A stimulated PBMC supernatants alterd rnorphology of cultured SCL-1 from a disorganized phenotype to a more organized phenotype. It is concluded that fibroblasts and PBMC may affect the growth and differentiation of SCL-1 via their mediators(cytokines)
Carcinoma, Squamous Cell* ; Cell Line* ; Concanavalin A ; Fibroblasts* ; Membranes ; Phenotype ; Skin

No abstract available.
Extremities*

Hypothalamic hamartomas are often associated with early onset gelastic seizures, precocious puberty, behavioral problem and suboptimal response to antiepiletptic drugs. Until now, four surgical options have been reported to reduce seizure by >50%. Surgical excision have good seizure outcome but postoperative complications were not infrequent, whether by pterional or transcallosal interforniceal approach. Radiosurgery is noninvasive alternative to resective surgery but the effect usually does not appear until several months later. Radiofrequency ablation is less invasive than surgical resection and its effect is immediate, but lacks long term follow-up data. It also requires three dimensional analysis of the lesion to enhance effi cacy and safety. As hypothalamic hamartoma is intrinsically epileptogenic and epileptogenic discharges spread from the lesion, blocking the seizure propagation through endoscopic disconnection is regarded as an effective and safer option. Surgical choice for a particular patient should take into account the hamartoma’s size, location, surgeon’s preferences, possible complication as well as the effect and risk of the various surgical methods. In the present review, open surgery, endoscopic disconnection, radiosurgery and radiofrequency ablation are discussed.