1.Using peniostium in modified dacryocystorhinosomy operation
Journal of Medical Research 1998;6(2):11-14
145 nasolacrimal obstruction patients (150 eyes) were treated with the modified dacryocystorhinostomy operation (using periostium). Group I: 45 cases with torn nasal-membrane. Group II: 145 cases with intact nasal-membrane. The general results are the same in both groups but the functional result of group II is much higher than that of group I. Thank to using periostium in operation, the treatment result is much increased. Six months after operation, the treatment result is stable. The technique is quite simple
Nasolacrimal Duct
;
Lacrimal Duct Obstruction
2.Treatment of nasolacrimal duct obstruction and lacrimal stenosid or obstruction by dacryorhinostomy
Journal of Vietnamese Medicine 1998;6(2):30-34
The study was done on 82 eyes having nasolacrimal duct obstruction and lacrimal stenosis or stenosis-obstruction. All were treated with dacryorhinostomy (DCR) and silicone intubation. The successful prevalence is 94.4%. Among them, the prevalence of functional success is 49.9%. The successful prevalence is increasing during the follow-up time. The intra-and post-operative complications are rare and mild. The causes and the form of obstruction are prognostic factors. The recurrent obstruction may occur when the silicone tube is still inside the canalcular
Nasolacrimal Duct
;
Airway Obstruction
;
therapy
3.The primary results of treatment of nasolacrymal duct obstruction in children by dacryorhinostomy
Journal of Vietnamese Medicine 1998;6(2):7-10
The study was done on 9 children aged from 4-16 with 10 eyes having congenital or traumatic naso-lacrymal duct obstruction. These eyes with associated canalicular stenosis or obstruction were treated by dacryorhinostomy (DCR) and silicone intubation in six months. The successful prevalence of DCR is as high as that of DCR on adult: 90%. Among them, 70% were functional success. One eye having recurrent obstruction is traumatic case. The failure occurred when silicone tube is still inside the canacular. DCR in children is not difficult than that in adult. The complication is rare and easy solved.
Nasolacrimal Duct
;
Lacrimal Duct Obstruction
;
therapy
;
child
4.Closing the gap for cervical cancer research in Vietnam: current perspectives and future opportunities: a report from the 5th Gynecologic Cancer InterGroup (GCIG) Cervical Cancer Research Network (CCRN) Education Symposium
Ngoc T.H. PHAN ; Quy T. TRAN ; Nhan P.T. NGUYEN ; Hang T. NGUYEN ; Linh D.N. TRAN ; Viet C. PHAM ; Katherine BENNETT ; Adriana CHÁVEZ-BLANCO ; Marie PLANTE ; Dong Hoon SUH ; Remi NOUT ; David S.P. TAN
Journal of Gynecologic Oncology 2023;34(5):e88-
5.Erratum: Closing the gap for cervical cancer research in Vietnam: current perspectives and future opportunities: a report from the 5th Gynecologic Cancer InterGroup (GCIG) Cervical Cancer Research Network (CCRN) Education Symposium
Ngoc T.H. PHAN ; Quy T. TRAN ; Nhan P.T. NGUYEN ; Hang T. NGUYEN ; Linh D.N. TRAN ; Viet C. PHAM ; Katherine BENNETT ; Adriana CHÁVEZ-BLANCO ; Marie PLANTE ; Fabrice R LECURU ; Dong Hoon SUH ; Remi NOUT ; David S.P. TAN
Journal of Gynecologic Oncology 2023;34(6):e89-
6.Production, purification and characterization of novel protease from Bacillus amyloliquefaciens D19 isolated in Vietnam
Tan Viet Pham ; Hanh Thi Dieu Nguyen ; Thi Luyen Bui ; Ngoc An Nguyen
Malaysian Journal of Microbiology 2024;20(no.1):85-93
Aims:
Microorganisms play a vital role in the breakdown of natural organic compounds and are valuable objects for worldwide enzyme production. The aim of this study was to identify favorable production conditions for Bacillus amyloliquefaciens D19 protease, followed by the purification and chemical characterization of this novel enzyme to assess its potential applications in various fields.
Methodology and results:
In this study, favorable conditions of protease production from B. amyloliquefaciens D19 were determined using a medium containing soluble starch (1.5%), earthworm extract (1.0%), yeast extract (0.5%), NaCl
(1.0%), at pH 7.0-8.0, 37 °C for 36 h with 150 rpm shaking condition. The protease was purified and had a molecular weight of about 23 kDa. The optimum condition for casein hydrolysis was at 40 °C and pH 6.5-7.0 in the presence of 1.0 mM Na+ or 5.0 mM Zn2+. The enzymatic activity was maintained at 75-100% at 30-50 °C and in pH 6.0-10.0. The values of Vmax and KM were also determined as 1547 U/mg and 6.33 mg/mL, respectively.
Conclusion, significance and impact of study
The identified optimal conditions will serve as the foundation for the production of the 23 kDa B. amyloliquefaciens D19 protease, one of the smallest proteases within the Bacillus genus. Moreover, its notable heat resistance, broad pH tolerance, high substrate catalysis and moderate substrate binding affinity make this enzyme a promising candidate for various applications in the food-feed and brewing industries.