Objective To approach the effects of the combination of simvastatin,clopidogrel and low molecular weight heparin on patients with unstable angina pectoris(UAP). Methods A total of 86 patients presented with UAP in Department of Internal Medicine of Tianjin Hongqiao Hospital were divided into control group and therapy group(each 43 cases)by random number table. The patients in the control group were treated with routine therapies such as oxygen,anti-platelet agents,nitrates,β-blockers,calcium channel antagonists,et al,while the patients in treatment group received besides the above conventional treatments,they additionally took simvastatin, clopidogrel orally and subcutaneous injection of low molecular weight heparin. The frequency of recurrence of angina, improvement of cardiac ischemia,the change of cholesterol,adverse reaction and the occurrence of complication were observed during 4 weeks of treatment. Results Compared to the control group,the frequency of angina(time/week:2.77±1.12 vs. 3.78±2.57,P<0.05)and consumption of nitroglycerin(mg/d:1.16±0.46 vs. 2.89±1.89,P<0.05) were obviously less,the improvement of cardiac ischemia(95.3%vs. 79.1%,P<0.05)and electrocardiogram(ECG:90.7%vs. 62.8%,P<0.05)was superior and the total effective rate was higher obviously in the therapy group. After treatment,plasma cholesterol levels were decreased obviously in treatment groups compared with before treatment (mmol/L:3.78±1.23 vs. 5.76±1.74,P<0.05),but the statististical difference in cholesterol levels in control group was found between before and after treatment(5.66±1.14 vs. 5.69±1.89,P>0.05). Before and after treatment,there were no significant changes of platelet count(PLT),activated partial thromboplastin time(APTT),liver functions and renal functions,and after treatment,bleeding and allergy serious adverse reactions were not seen in patients of both groups. Myocardial infarction occurred in 2 patients in the control group. Conclusion The addition of combined application of simvastatin,clopidogrel and low molecular weight heparin onto routine therapies has better effects for treatment of UAP.

Objective To investigate the effects of C-peptide on apoptosis induced by high glucose and analyze the relationship between the apoptosis and expression of eNOS in HUVEC. Methods We determined the HUVEC apoptosis by Annexin-V, the expression of eNOS mRNA of HUVEC by RT-PCR, the apoptotic quantity of HUVEC by DNA fragment ELISA. Results High level of glucose could increase HUVEC apoptosis (P<0.01) and reduce the expression of eNOS. After adding physiological or high concentration of C-peptide, the apoptosis decreased and the expressions of eNOS increased markedly, with the relationship coefficient of -0.854 (P<0.01). Conclusion High glucose may induce apoptosis. Physiological or high concentration of C-peptide may reduce the apoptosis induced by hyperglycemia, the relationship between apoptosis and eNOS is negative.

Objective: To investigate the current situation of consumption and development tendency of antitumor Chinese herbal injections in Wuhan area. Methods:The analytical method of the defined daily dose ( DDD) recommended by WHO was used to ana-lyze the application of antitumor Chinese herbal injections in 32 hospitals of Wuhan area during the period of 2010-2012. Results:The consumption sum and the DDDs of antitumor Chinese herbal injections were increased year after year, while the daily drug cost ( DDC) was stable in general. The sort of consumption was generally in accordance with the sequence of DDDs. Conclusion:The status quo of antitumor Chinese herbal injections during the period of 2010-2012 is in line with the characteristics of clinical application, indicating the use is rational.

A lot of evidence shows that autophagy is involved in the occurrence and development of cerebral ischemia - reperfusion. Autophagy and its related signal transduction pathways may be an effective therapeutic target for ischemic brain injury. This article reviews related signal transduction pathways of autophagy regulation after cerebral ischemia.

After the concept of symptoms in traditional Chinese medicine was defined, the classification of symp-toms in traditional Chinese medicine and the characteristics of clinical diagnosis and treatment in traditional Chinese medicine were described .It was suggested that the symptoms in traditional Chinese medicine should be classified into 3 kinds, namely symptoms and signs according to the 4 diagnostic methods, symptoms and signs according to the diagnostic devices, symptoms and signs according to the physical constitutions of patients in order to lay a foun-dation for improving the classification in clinical term system of traditional Chinese medicine.

Humans ; Optical Imaging

Objective To explore the use of urine LH test paper associating basal body temperature in ovulation monitoring and conception guiding in anovulatory infertility.Methods We used clomiphene to treat 100 anovulatory infertility patients.Then we recorded basal body temperature and used urine LH test paper in morning to determine whethere there is urine LH peak between the 12th to 20th day of menstrual cycle.If the patient become pregnant,we stop observation.If not,we observed them until the third treatment cycle completed.Result Before treatment,both the urine LH test paper and basal body temperature showed there was no ovulation.After treatmend,we observed 199 menstrual cycle.There were urine LH peak in 149 menstrual cycle.And there were double phase body temperature in 158 treat cycle.The lowest body temperature appeared in the urine LH peak day or after it.71.1%of the lowest body temperature emerged in one or two days after the urine LH peak appeared.76 patients in our study become pregnant,pregnancy rate is 76%.Conclusion Urine LH test paper associating basal body temperature is convenient and easy tO learn.It is a good way in ovulation monitoring and conception guiding.

Objective To explore the relationship between the expression changes of cytokines, inter-leukin-6(IL-6), interleukin-8(IL-8), and tumor necrosis factor-α (TNF-α), and the wound time following explosive injury to rabbit’s chest. Methods The rabbit’s model of explosive injury was established. The expression levels of IL-6、 IL-8 and TNF-α in the plasma were detected by ELISA method at different wound time (0.5-12 h). Results The level of IL-6 increased at 3 h after wounding and reached peak at 6 h. The level of IL-8 increased at 1 h and reached peak at 6 h. The level of TNF-α increased at 0.5 h and reached peak at 3 h. Conclusion IL-6, IL-8 and TNF-α have a time-related expression after explo-sive injury.

Objective To investigate the isoniazid-dependence of clinical isolated strains of M.tuberculosis.Methods The double-phase Kuang's agar medium was employed to isolate M.tuberculosis from the patient's sputum,and its drug-resistance and drug-dependence were determined.Results 184 clinical isolates of isoniazid-dependent M.tuberculosis were grouped as follows.Type A:the strains grew faster and more vigorous in drug tubes than in control tubes,and there were 12 strains(6.5%)which grew more colonies in high concentration tubes than in low concentration tubes;Type B:the strains grew faster and more vigorous in drug tubes than in control tubes,and there were 10 strains(5.4%)which grew more colonies in low concentration tubes than in high concentration tubes;Type C:the strains grew faster and more vigorous in low concentration tubes than in control tubes,and there were 15 strains(8.15%)which grew less colonies in high concentration tubes than in control tubes;Type D:the strains grew faster and more vigorous in high concentration tubes than in control tubes,and there were 4 strains(2.2%)which grew less colonies in low concentration tubes than in control tubes.The isoniazid-dependent strains covered 22.3%(41/184)of the total clinical isolates.Conclusion The isoniazid-dependent strains did exist in the clinical isolates of M.tuberculosis,and different features of dependence have been detected.

Objective To investigate the feasibility of multiplex PCR for rapid identification of Mycobacterium tuberculosis and non-tuberculosis Mycobacteria. Methods According to MTP40 gene sequence of Mycobacterium tuberculosis, 32kD gene sequence of Mycobacterium and IS6110 insertion sequence gene sequence of Mycobacterium tuberculosis complex, three specific pairs of primers (PT1-PT2, MT1-MT2 and IS5-IS6) for Mycobacterium were designed, and the target DNA for MTP40, 32kD and IS6110 was 396bp, 506bp and 984bp, respectively. The genome of 92 Mycobacterium tuberculosis clinically isolated strains and 5 non-tuberculosis Mycobacteria clinical strains were amplified in the same system, and the results were compared with reference strains. Results Among 92 clinical strains of Mycobacterium tuberculosis, the DNA fragments of 396bp, 506bp and 984bp were found in 90 Mycobacterium tuberculosis clinical strains, as well as in the reference strain H37Rv; the sensitivity of multiplex PCR for Mycobacterium tuberculosis was 97.8%, and the specificity was 100.0%. The DNA fragments of 506bp were all found in 5 non-tuberculosis Mycobacteria clinical strains, the sensitivity and specificity for non-tuberculosis Mycobacterium were both 100.0%. Conclusion The multiplex PCR is a rapid, sensitive and specific method for identification of Mycobacterium tuberculosis and non-tuberculosis Mycobacteria, and it may provide an effective way for clinical diagnosis of Mycobacterium tuberculosis and non-tuberculosis Mycobacteria, therefore useful in clinical application.