Objective:To investigate the effects of trimetazidine on cardiac systolic and diastolic function in patients with chronic congestive heart failure due to coronary artery disease.Methods:45 patients with chronic congestive heart failure due to coronary artery disease were divided into two groups,23 patients in trimetazidine group and 22 in placebo-control group.The echocardiogram indices of cardiac function of patients in two groups-SV,CO,CI,LVEF,VA,VE and VA/VE were measured before and after treatment.Results:The indexes SV,CO,CI and LVEF were significantly higher in trimetazidine group than those in control group(P0.05).Conclusion:Trimetazidine can improve systolic function of ischemic heart by improving the energy metabolism of ischemic heart.

Objective:To investigate the relationship among serum uric acid,C-reactive protein and the degree of carotid artery atherosclerosis in patients with essential hypetension.Methods:122 patients with essential hypertension were divided into intima-media thickness group(Ⅰ),plaque development group(Ⅱ) and vessel stenosis group(Ⅲ).Concentration of serum uric acid and C-reactive protein were measured in each patient and the relationship between serum uric acid and C-reactive protein was observed.Results:The concentrations of serum uric acid and C-reactive protein in group Ⅱ and Ⅲ were positively connected with pathologic changes of carotid artery atherosclerosis( P

(0.05)).Conclusions PE is an effective form of treatment for recurrent FSGS by removing circulating factor,especially if initiated early.Failure to achieve remissions and maintain stable remission after the initial period of PE imply a poor outcome and glomeruli lesion been sclerosis.Therefore,PE should be performed as early as possible until completely remissions.

Objective To investigate the impact of different surgical methods and different doses of euthyrox on bone mineral density.Methods A sample of 40 patients received thyroid cancer operation (excised affected side leaf,aisthmus,most of offside and neck VI lymph node dissection)and 40 patients received nodular goiter operation (excised affected side leaf)were collected,the dose of euthyrox taken in was 85 .2μg in thyroid cancer patients and 36.2μg in nodular goiter patients daily,the bone mineral density 6 month and 1 year after operation was detected. Results There was no difference of BMD between the two group 6 month after operation (P>0.05),but BMD of the observation group was (0.64 ±0.28)g/m2,which was lower than that of control group (t=2.235,P=0.03).Com-pared with pre-operation,BMD of control group had no change and the observation group appeared drop 1 year after operation.Conclusion Patients received thyroid cancer taking in euthyrox may show BMD reduced,especial 1 year after operation,doctors should be on the alert and supplement calcium appropriately.

A lot of children with congenital heart disease have an abnormal development of nervous system. A variety of biochemical and physical examination indices can be used to predict adverse outcome of neurodevelopment caused by congenital heart disease and they contribute to early intervention and improvement of neurodevelopmental disorders. In recent years, new progresses in the laboratory tests, neurological physiccal examination and perioperative management of the neurodevelopmental abnormalities caused by congenital heart disease have been made. The predictive factors of abnormal neurodevelopment caused by congenital heart disease were reviewed in this article.

Objective To explore whether Islet-1 gene of the rat could induce NSCs to differentiate into cholinergic neurons. Methods NSCs were transduced with Islet-1 recombinant retroviral expression vector.The protein expression of Islet-1 gene in NSCs was detected by immunofluorescence histochemical method.The ability of NSCs to differentiate into ChAT positive cells was observed in vivo and in vitro. Results The numbers of ChAT positive cells were significantly increased in the group of NSCs modified with Islet-1 compared with the control group in vitro.NSCs modified with Islet-1 could differentiate into ChAT positive cells when they were grafted into the corpus striatum of the adult rat brain.Conclusion Islet-1 gene could induce NSCs to differentiate into cholinergic neurons.

Objective An effective method for separating coumarin from Artemisia capillaris by solvent sublation was established.Methods The effects of sublation solvent,the concentration of sample solution,N2 gas flow rate,pH value of solution,sublation time,and electrolyte NaCl etc.on the sublation efficiency were investigated and the optimal conditions of the solvent sublation were obtained.Results In the optimal conditions,the results of the solvent sublation were evaluated and compared with the solvent extraction.Conclusion The experimental results show that this method is simple and rapid,and the efficiency of coumarin by solvent sublation is far better than that by the solvent extraction.

Objective To construct a recombinant plasmid pLEGFP-mIFN? for developing the genetical therapy of tumor by stem cells.Methods PCR was used to amplify the mIFN? gene from plasmid pcDNA3.1-mIFN? in which the mIFN? was cloned.A new plasmid,pLEGFP-mIFN?,was then constructed by inserting the amplified mIFN? gene into pLEGFP-C1.Restriction analysis and sequencing were used to confirm the structure of pLEGFP-mIFN?.Neural stem cells were transfected by supernatant of PA317.Results A DNA fragment in size of 500 bp was amplified.Restriction analysis showed that the amplified gene was inserted into pLEGFP-C1 correctly.(Conclusion The) plasmid pLEGFP-mIFN? is constructed successfully,and it can transfect neural stem cells.

Objective To observe the inhibitory effect of LaCl_3 on the growth of human hepatocellular carcinoma cell lines SMMC-7721 and its mechanism.Methods The cells were treated with different doses of LaCl_3 and the growth curve,colony inhibitory rates,the levels of AFP of cultured cells were detected at 1,3,5 and 7 days after they were dealed with LaCl_3.The changes of cell cycle were detected by flow cytometry and the expressions of cell cycle protein CyclinD_1,PCNA,CDK_4 and P16 were observed by immunocytochemistry staining.Results MTT test showed that SMMC-7721 cell proliterating activity was obviously inhibited by LaCl_3 in a time and dose related manner at concentrations of 0.50 and 1.00 mmol?L~(-1).The colony forming inhibitory rate of 0.10,0.50 and(1.00 mmol?L~(-1))LaCl_3 were 51%, 67% and 97% (P

OBJECTIVE To investigate the status of community health services and offer a scientific basis for making a direction of community to control the infective disease.METHODS Totally 1503 outpatients were investigated retrospectively for infective diseases in community.RESULTS The average age of these patients was 65,the number of females was much more than males.The most frequently infective diseases were upper respiratory tract infection,bronchitis,periodontitis,etc.CONCLUSIONS It′s necessary to strengthen the community health service and to establish an effective system of health service.The patients must cultivate a health habit of life,they not only need in time diagnosis and treatment,but also enhance own knowledge about health and disease prevention.