No abstract available.
Animals ; Hypokinesia* ; Immobilization* ; Muscle, Skeletal* ; Rats*

Protein expression patterns of matrix metalloproteinases (MMPs) were examined in mouse reproductive organs during estrous cycle. Estrous cycle was classified into diestrus, proestrus, estrus or metestus and MMP expression was analyzed by zymography using gelatin as a substrate. Uterine fluid (UF) obtained both at diestrus and proestrus exhibited 4 major MMPs including 106kDa, 64kDa, 62kDa and 59kDa gelatinases. However, in UF at estrus, the gelatinolytic activity of 64kDa MMP disappeared and that of 106kDa and 62kDa MMPs dramatically decreased. At metestrus, 64kDa MMP activity reappeared and 106kDa and 62kDa MMP exhibited increased activities such that the band intensity of 106kDa was comparable to that in UF at diestrus. Gelatinolytic activity of 59kDa MMP was not changed throughout the cycle. Both ovarian and oviductal tissue homogenate revealed 4 MMPs which corresponded to the 4 MMPs of UF. However, unlike UF MMPs, gelatinolytic activity of these MMPs did not show distinct changes throughout the cycle. Either an inhibitor of MMP, 1, 10-phenanthroline, or a metal chelator, EDTA, abolished the appearance of the above MMP activities in gelatinated gel whereas a serine proteinase inhibitor, phenylmethylsulfonyl fluoride, failed to inhibit the appearance of MMP activities, proving that gelatinolytic activity of the above reproductive tissues were due to the enzymatic activity of MMP. When gelatinolytic activity of mouse serum was examined, it revealed 5 MMPs (131kDa, 106kDa, 89kDa, 64kDa and 62kDa bands) and one gelatinase (84kDa) band. From these results, it is concluded that the protein expression of MMPs of mouse reproductive organs, particularly uterus, is temporally regulated during estrous cycle and uterine 106kDa, 64kDa and 62kDa MMP,3 are suggested to play an important role in cyclic tissue remodeling of mouse uterus.
Animals ; Diestrus ; Edetic Acid ; Estrous Cycle* ; Estrus ; Female ; Gelatin ; Gelatinases ; Matrix Metalloproteinases* ; Metestrus ; Mice* ; Oviducts ; Phenylmethylsulfonyl Fluoride ; Proestrus ; Serine Proteases ; Uterus

BACKGROUND: The potential adverse effects of ketamine in neurosurgical anesthesia have been well established. However, the effects of ketamine on intracranial pressure (ICP) and hemodynamics during general anesthesia remain unclear. The purpose of this study was to assess the effects of ketamine on hemodynamics and ICP in anesthetized, ventilated rabbits. METHODS: Thirty rabbits were divided into three groups: Group 1 (n=10) received 1 ml/kg normal saline iv; Group 2 (n=10) received 0.5 mg/kg ketamine iv; Group 3 (n=10) received 1.0 mg/kg ketamine iv. After induction with thiopental, anesthesia was maintained with isoflurane and nitrous oxide in oxygen. During controlled ventilation, ICP, mean arterial pressure (MAP), cerebral perfusion pressure (CPP) and heart rate (HR) were measured. The ICP was measured using Ladd ICP monitoring system. All variables were evaluated at baseline and for 30 min following ketamine. RESULTS: In group 1, ICP, MAP, CPP and HR were unchanged over the course of the study. In group 2, ICP, MAP and CPP were unchanged. HR increased at 1, 3 and 5 min (p<0.01), 10 and 20 min (p<0.05) after injection. In group 3, ICP, MAP and CPP increased at 1 and 3 min (p<0.01) after injection. HR increased at 1, 3 and 10 min (p<0.01), 5 min (p<0.05) after injection. CONCLUSIONS: These results suggest that 0.5 and 1.0 mg/kg of ketamine don't significantly affect the hemodynamics and ICP in anesthetized, mechanically ventilated rabbits.
Anesthesia* ; Anesthesia, General ; Arterial Pressure ; Heart Rate ; Hemodynamics* ; Intracranial Pressure* ; Isoflurane ; Ketamine* ; Nitrous Oxide ; Oxygen ; Perfusion ; Rabbits* ; Thiopental ; Ventilation

No abstract available.

This study was performed to observe the sequential charge of the morpholcgic characteristics in experimentally induced herpes simplex keratitis. Duration and morphology of corneal lesion following infection of rabbit cornea with the Kos strain of HSV-I were followed by a daily slit-lamp examination. Three types of virus inoculation methods were used such as scratching, deepithelialization, and intrastromal injection. Herpetic corneal lesions appeared 24 hours after inoculation with punctate and dendritic figures. They persisted up to 14 or 15 days. The characteristic finding in punctate herpetic keratitis was grouped, round-shaped, punctate lesion. When scratching method was emplyed, the most remarkable finding was the discontinuity of the lesion occurred along the scratching wound at relatively regular intervals. There was no difference in lesional morphology and duration between three inoculation methods.
Cornea ; Keratitis* ; Keratitis, Herpetic ; Wounds and Injuries

The linear scratching wound was made gently on the corneal epithelium of rabbit with 21 gauge needle under an operating microscope. Impression cytology was performed at 30 minutes, 1, 2, 3 and 4 hour and 1, 2, 3 and 4 day after 0.5% tetracaine drops under an operating microscope. The filter paper was stained with hematoxylin and eosin. At 30 minute post-scratching, a few polymorphonuclear leukocytes appeared on the scratched cornea at 3 eyes (30%). At 3 and 4 hour, numerous polymorphonuclear leukocytes with corneal epithelial cells appeared on the scratched conea. By 3 day, no inflammatory cells were shown on the filter paper in all eyes. These findings suggest that the polymorphonuclear leukocytes could infiltrate on the corneal lesion at 30 minute post-scratching and the inflammatory cells might act even on the minute corneal lesion such as corneal erosion.
Cornea ; Eosine Yellowish-(YS) ; Epithelial Cells ; Epithelium, Corneal ; Hematoxylin ; Needles ; Neutrophils* ; Tetracaine ; Wounds and Injuries*

A 18-month-old girl was seen because of an yellowish brown papular eruptions on the face, earlobes and neck of one year duration. A skin biopsy specimen revealed circumscribed cellular infiltrates composed of predorninantly pleornorphic histiocytes. Electron microscopy of biopsy material disclosed numerous worm like particles and coated vescles in limited area of the cell cytoplasm, consistent with the findinga described in benign cephalic histiocytosis. After six months of her first visit, the individual papules became flattened.
Biopsy ; Coated Vesicles ; Cytoplasm ; Female ; Histiocytes ; Histiocytosis* ; Humans ; Infant ; Microscopy, Electron ; Neck ; Skin

No abstract available.
Kidney Transplantation*

The whole retina, except for the medullary fiber zone in a rabbit eye, is supplied by choroidal circulation. Therefore, the histopathological changes of the sensory retina due to choroidal circulatory disturbance in rabbits may be comparable to that of the human sensory retina in the case of ophthalmic artery occlusion. This study was carried out to evaluate the histopathological changes of the ischemic retina secondary to the occlusion of choroidal circulation. The experimental occlusion of all posterior ciliary arteries and anterior ciliary arteries in the horizontal rectus muscle of rabbit eyes was performed and the subsequent histopathological changes of the sensory retina were observed by transmission electron microscopy. The morphological changes of the sensory retina following the occlusion of the ciliary arterial system are as follows: severe loss of the inner and outer segments of the photoreceptor, mild to moderate degeneration of the ganglion cells, and excellent preservation of the Muller's cell fibers and the extension of the cytoplasmic villous processes to the cytoplasmic vacuolar spaces of other degenerated cells. These findings indicate that the Muller's fibers in the ischemic condition of retina might contribute to the formation of gliosis or scarring of a damaged retina.
Animals ; Arterial Occlusive Diseases/*complications ; Arteries ; Choroid/*blood supply ; Ciliary Body/*blood supply ; Ischemia/*etiology/pathology ; Rabbits ; Retina/*ultrastructure ; *Retinal Vessels

Inclusion bodies containing glycogen granules are characteristic feature of infected cells with Chlamydia trachomatis(C. trachomatis). In this study, we investigated the serial changes of the glycogen granules in a rabbit conjunctival epithelial cells co-cultivated with C. trachomatis.The epithelial cells isolated grom conjunctiva of rabbit were initially cultured for 3 weeks. After the cells had attained confluency, they were infected with C. trachomatis serotype D. After co-cultivation for 24, 48 and 96 hours, electronmicroscopic study was performed.After co-cultivation for 24 hours, a few glycogen granules were observed within the premature inclusion body containing some elementary bodies and reticulate bodies. After 48 hours of co-cultivation, typical inclusion bodies were observed and numerous electron-dense glycogen granules predominated around reticulate bodies. After 96 hours of co-cultivation, scattered glycogen granules were observed in granular pattern within the expanding inclusion occupying the bulk of the host cell cytoplasm.These results suggest that glycogen granules are closely related to the developmental cycle of the C. trachomatis and they may be an energy source for active multiplication of the reticulate bodies.
Chlamydia trachomatis* ; Chlamydia* ; Conjunctiva ; Epithelial Cells* ; Glycogen* ; Inclusion Bodies