Animals ; Cell Separation ; methods ; Female ; Male ; Myocytes, Cardiac ; cytology ; metabolism ; Proto-Oncogene Proteins c-kit ; metabolism ; Rats ; Rats, Sprague-Dawley ; Stem Cells ; cytology ; metabolism

Objective:To investigate the levels of the specific antibody in the serum of convalescent SARS patients.Methods:The specific antibody in the serum of convalescent SARS patients were detected by indirect ELISA and double antigen sandwich method.Results:The levels of the specific IgG antibody in the serum of convalescent SARS patients is up to the maximum in the fifth week, the levels of the specific IgM is up to the maximum in the third week. The levels of the specific IgG in the serum of convalescent SARS patients is 1-7 times than that of the specific IgM. The positive detection rate of the specific antibody during 5-7 weeks in the serum of convalescent SARS patients is up to 100%.Conclusion:The levels of the specific antibody in the serum of convalescent SARS patients is up to the maximum in the fifth week, and the positive detection rate of the specific antibody is 100%.

Objective To explore the clinical effectiveness and safety of percutaneous vertebral augmentation with the Vessel-X bone void filling container system (Vesselplasty). Methods Three cases of fresh osteoporotic vertebral compression fracture were treated with Vesselplasty. After procedure, the pain relief, the fracture reduction, and the cement distribution in the vertebra were observed. Results All the 3 cases were treated with the unipediclar injection technique. The operative time was 45, 32 and 30 min, respectively. The hemorrhage volume was

Objective To investigate the drug tolerance of Acinetobacter baumanni and Stenotrophomonas maltophilia . Methods The antimicrobial susceptibility tests for 275 isolates of Acinetobacter baumanni and 107 isolates of Stenotrophomonas maltophilia from 2000 to 2004 were measured by MicroScan WarkAway 96 The chemotherapeutic effects of 18 cases of sequent infection with Acinetobacter baumanni and Stenotrophomonas maltophilia was analyzed. Results The resistance of the two species of bacteria to twelve antibiotics increased obviously during the last five years, especially from 2000 to 2001. The resistant rate of Acinetobacter baumanni to Cefepime、Cefotaxime、Ceftazidime and Ceftriaxonewas was 20~50% in 2000, but raised to 70%~81% in 2004. For Acinetobacter baumanni to Amikacin、Amp/sulbac、Ciprofloxacin、Gentamicin、Tobramycin and Trimeth/Sulfa, the resistant rate was 20%~40% in 2000, while 63~86% in 2004. The lowest resistant rate was to Imipenem, only 7% or so. The resistant rate of Stenotrophomonas maltophilia to Ciprofloxacin、Ceftazidime、ceftriaxone and Tobramycin was 25%、50%、0% and 0%, respectively, in 2000, but in 2004 year, was 76%、76%、95% and 95%, respectively. Stenotrophomonas maltophilia showed a high drug tolerance to other antibiotics. Conclusion To strengthen the monitoring of the antibiotic resistance of Acinetobacter baumanni and Stenotrophomonas maltophilia and the monitoring of sequential infection in Acinetobacter baumanni and Stenotrophomonas maltophilia is very important for clinic so as to choose antibiotic rationally and improve curative effect

Objective The aim of the present study was to assess the changes of IGF-1,IGF-2,IGFBP-1 and IGFBP-3 in diabetes nephropathy,the relationships between IGFs,IGFBPs and ?_1-,?_2- microglobulin.Methods A total of 130 type-2 diabetic participants(male:52,female:78)aged 37~75 yeares in Guangdong Provincial People's Hospital were enrolled from Feb.2000 to May 2003.Serum IGF-1,IGF-2,IGF binding protein 1(IGFBP-1),IGFBP-3,creatinine(Cr),cholesterol,triglycerides,HDL,LDL,apolipoprotein B100(apoB100),apoA,HbA_1c,fasting and postprandial C-peptide,serum and urine ?_1-MG and ?_2-MG were measured.Patients were divided into clinical albuminuria(DN,n=24),microalbuminuria(MA,n=40),and normal(NOR,n=66)groups according sCr and urine Alb/Cr ratio.Results In multiple linear regression analysis,determinants of S ?_1-MG were IGF-2 、TC and age(r=0.492,P=0.0001);U ?_1-MG were IGF-2、age(r=0.307,P=0.007); S ?_2-MG were IGFBP-1、HDL and age(r=0.528,P=0.0001);U ?_2-MG were IGFBP-1、CP120 and age(r=0.407,P=0.0001);s-Cr were IGFBP-1、CP0 and age(r=0.499,P=0.0001)。Serum IGF-2 and IGFBP-1 were significantly increased in DN group[IGF-2:(889.48?65.94)?g/L vs (711.57?28.73)?g/L,P=0.03;IGFBP-1:(94.91?17.48)?g/L vs(54.81?5.04)?g/L,P=0.009].Conclusion Serum IGFBP-1 and IGF-2 are significantly increased in type 2 diabetes with nephropathy.The disorders of IGFs and lipid metabolism may contribute to the development of diabetic nephropathy.

Objective To investigate the isoniazid-dependence of clinical isolated strains of M.tuberculosis.Methods The double-phase Kuang's agar medium was employed to isolate M.tuberculosis from the patient's sputum,and its drug-resistance and drug-dependence were determined.Results 184 clinical isolates of isoniazid-dependent M.tuberculosis were grouped as follows.Type A:the strains grew faster and more vigorous in drug tubes than in control tubes,and there were 12 strains(6.5%)which grew more colonies in high concentration tubes than in low concentration tubes;Type B:the strains grew faster and more vigorous in drug tubes than in control tubes,and there were 10 strains(5.4%)which grew more colonies in low concentration tubes than in high concentration tubes;Type C:the strains grew faster and more vigorous in low concentration tubes than in control tubes,and there were 15 strains(8.15%)which grew less colonies in high concentration tubes than in control tubes;Type D:the strains grew faster and more vigorous in high concentration tubes than in control tubes,and there were 4 strains(2.2%)which grew less colonies in low concentration tubes than in control tubes.The isoniazid-dependent strains covered 22.3%(41/184)of the total clinical isolates.Conclusion The isoniazid-dependent strains did exist in the clinical isolates of M.tuberculosis,and different features of dependence have been detected.

The results of drug activity of singe drug and multidrug combination susceptibility tests showed the coincidence rate between the two kinds of tests was above 95% . The results also indicated that the chemotherapy program consisted of resistant drugs exhibited an inhibitory effects to different extents on the growth of 40% strains in vitro. Using two resistant drugs and one susceptible drug in the susceptibility test of three drug combination assay the pathogenic strain was still susceptible. It is proved that the extents of susceptibility of pathogenic strains to chemotherapy programs could be accurately calculated by the time of initial growth , the colony count and the colony size in multidrug combination susceptibility test. The results suggested Kuang′s agar medium should be adapted in the drug susceptibility test and the multidrug combination susceptibility test should be carried out for multidrug resistant strains of Mycobacterium tuberculosis.

PURPOSE To provide evidence for the relationship between degree of invasion and tumor metastasis. The tumor cells of transplantable mouse histiocytic sarcoma (L1) were inoculated at the right hind footpads of inbred 615-strain mice. METHODS Once bearing the tumor, all the mice were sacrificed respectively on the 1st. 3th, 5th. 10th, 20th, 30th, and 40th day as to observe the degree of tumor invasion and the process of tumor metastasis. RESULTS When the degree of tumor invasion was grade Ⅲ or Ⅳ , the metastasis of tumor cells was found earliest in draining lymph nodes. However, the tumor metastasis in lung appeared later than in lymph nodes. CONCLUSION According to the time of tumor growth and degree of invasion and metastasis, the authors suggest a new classification for cancer staging, namely, latent, invasive and metastatic stages. During the stage of tumor invasion, it is redivided into early, middle and late invasive phases. During the stage of tumor metastasis, it is redivided into early, middle and late metastatic phases.

Objective To investigate the feasibility of multiplex PCR for rapid identification of Mycobacterium tuberculosis and non-tuberculosis Mycobacteria. Methods According to MTP40 gene sequence of Mycobacterium tuberculosis, 32kD gene sequence of Mycobacterium and IS6110 insertion sequence gene sequence of Mycobacterium tuberculosis complex, three specific pairs of primers (PT1-PT2, MT1-MT2 and IS5-IS6) for Mycobacterium were designed, and the target DNA for MTP40, 32kD and IS6110 was 396bp, 506bp and 984bp, respectively. The genome of 92 Mycobacterium tuberculosis clinically isolated strains and 5 non-tuberculosis Mycobacteria clinical strains were amplified in the same system, and the results were compared with reference strains. Results Among 92 clinical strains of Mycobacterium tuberculosis, the DNA fragments of 396bp, 506bp and 984bp were found in 90 Mycobacterium tuberculosis clinical strains, as well as in the reference strain H37Rv; the sensitivity of multiplex PCR for Mycobacterium tuberculosis was 97.8%, and the specificity was 100.0%. The DNA fragments of 506bp were all found in 5 non-tuberculosis Mycobacteria clinical strains, the sensitivity and specificity for non-tuberculosis Mycobacterium were both 100.0%. Conclusion The multiplex PCR is a rapid, sensitive and specific method for identification of Mycobacterium tuberculosis and non-tuberculosis Mycobacteria, and it may provide an effective way for clinical diagnosis of Mycobacterium tuberculosis and non-tuberculosis Mycobacteria, therefore useful in clinical application.

BACKGROUND: Temporal cortex is related to human and animal's learning and memory capability, it not only accepts the casting fibers of auditory sense, but also closely connects with many brain areas where are related to spatial cognition.OBJECTIVE: To observe the improving effect of thrombolytic capsule on spatial cognitive capability of rats with lateral temporal cortical infarction DESIGN: Completely randomized controlled study SETTING: Department of Neurology, the 175th Hospital of Chinese PLA;Department of Neurology, General Hospital of Chinese PLA; Institute of Psychology of Chinese Academy of Science MATERIALS: This experiment was carried out at Brain Function Laboratory of Institute of Psychology of Chinese Academy of Science from August 1998 to February 1999, 28 SD rats were randomly divided into 3 groups,namely brain stroke group with 10 rats, treatment group with 10 rats and sham operation group with 8 rats.METHODS: Brain infarction model was established on SD rats by using three-dimensional directional photochemistry induction. Rats were anaesthetized with hydrochloride and intravenously injected of rose pink solution in dosage of 20 mg/kg from tail vein. Then incision was made on the left temporal scalp to expose temporal bone, cold photosource with the guidance of optical fiber was used for directional illumination for 20 minutes in area of 2 mm2. Twenty minutes later, rats in treatment group were given intraperitoneal injection of thrombolytic capsule physiological saline of 40 g/L in dosage of 200 mg/kg, which replaced by the same volume of physiological saline in brain stroke group. Rats in sham-operation group received the same operative lamination and injected with the same volume of physiological saline from tail vein and intraperitoneal cavity.MAIN OUTCOME MEASURES: Achievement of Morris water labyrinth(edge type scored 1, randomize type of 2, trend type of 3 and straight-line type of 4).age response time: it was shortened in treatment group and sham operation group than brain infarction group [ on 1st day: 58,50,65 s; 2nd day: 24,27,46 s; 3rd day: 14,17,20 s; 4th day: 11,9,15 s, (P ＜ 0.01 )], but it was similar ing strategy grades: on the 1st day, scores were slightly higher in treatment group and sham operation group than infarction group, but were instable (F=1.167, P ＞ 0.05). On the 2nd day, it was higher in sham operation group than brain stroke group[2.9,2.3, (F=5.5 ,P ＜ 0.05)], but it was similar between treatment group and infarction group[2.6,2.3 scores, (F=3.34,P ＞ 0.05)]. on the 3rd and 4th day, it was higher in treatment group than infarction group [ 3.5,2.7 minutes; 3.7,3.3 minutes, (F=8.92, 14, P ＞ 0.05)]and similar between treatment group and sham operation group(P ＞ 0.05).CONCLUSION: Rat response time was reduced by thrombolytic capsule during the early time of cerebral infarction, changing searching tactics gradually from randomized and edge type to tendency type and straight line type, suggesting thrombolytic capsule can improve the post-ischemia cognitive function, raise training record of cerebral infarctional rats.