Peterson's hernia is an internal hernia that can occur after Roux-en-Y anastomosis. It often accompanies small bowel volvulus and is prone to strangulation. Reconstruction of intestinal continuity after massive small bowel resection in a patient who undergoes near total gastrectomy and Roux-en-Y anastomosis can be difficult. A 74-year-old man who had undergone a near total gastrectomy and Roux-en-Y gastrojejunostomy for stomach cancer presented with abdominal pain. The preoperative computed tomography showed strangulated small bowel volvulus. During the emergent laparotomy, we found a strangulated Peterson's hernia with small bowel volvulus. After resection of the necrotized intestine, we made a new Roux-en-Y anastomosis connecting the remnant stomach and the jejunum with a transverse colon segment. We were safely able to connect the remnant stomach and the jejunum by making a new Roux-en-Y anastomosis utilizing a transverse colon segment as a new Roux-limb by two stage operation.
Abdominal Pain ; Aged ; Anastomosis, Roux-en-Y ; Colon, Transverse* ; Extremities* ; Gastrectomy ; Gastric Bypass ; Gastric Stump ; Hernia* ; Humans ; Intestinal Volvulus* ; Intestines ; Jejunum ; Laparotomy ; Stomach Neoplasms

PURPOSE: DNA mismatch repair gene is responsible for hereditary nonpolyposis colorectal cancer. But it is not well known its role in sporadic colorectal cancer patients. We analysed normal hMSH2, hMLH1 protein expression in colorectal adenocarcinoma tissues and corresponding normal tissues to find out the role of mismatch repair gene in sporadic colorectal cancer by Western blotting. METHODS: Normal hMSH2 and hMLH1 protein expression was studied on 25 colorectal cancer and corresponding normal tissue by Western blot with hMSH2 and hMLH1 monoclonal antibody. Normal protein band was expressed on 100 kD in hMSH2 and 87 kD in hMLH1. SW480 and LoVo cell line was used as positive and negative control for hMSH2 and LoVo and SW480 as positive and negative for hMLH1. And we analysed the relation between the hMSH2, hMLH1 protein expression and clinicopathological parameters. RESULTS: It was 2 cases (8%) that both hMSH2 and hMLH1 protein expression was not observed. Three cases (12%) were negative for hMSH2 and 2 cases (8%) for hMLH1. One or both hMSH2, hMLH1 protein expression was not observed in 7 cases (28%) in total. There was no correlation for proximal occurrence (25% vs 35%), young age (37.5% vs 23.5%) and lymph node metastasis (50% vs 47%). But poorly and mucinous differentiation was regarded as having relation with negative expression of hMSH2 and hMLH1 (50% vs 17.6%) but not significant statistically. CONCLUSION: Sporadic colorectal cancer with negative expression of normal hMSH2 and hMLH1 protein showed no relation to younger age, proximal site preference and lymph node metastasis. But it was suggested that mismatch repair gene protein was involved in cancer cell differentiation in sporadic colorectal cancer.
Adenocarcinoma ; Blotting, Western ; Cell Differentiation ; Cell Line ; Colorectal Neoplasms* ; Colorectal Neoplasms, Hereditary Nonpolyposis ; DNA Mismatch Repair ; Humans ; Lymph Nodes ; Mucins ; Neoplasm Metastasis

Metaplastic carcinomas (carcinomas with metaplasia) of the breast are difficult to accurately diagnose and classify because of their rarity and varied histologic patterns. Cystic change can be encountered in mammary carcinoma, especially in carcinoma with squamous metaplasia, but are rare in pseudosarcomatous metaplastic carcinoma. We recently experienced a case of pseduosarcomatous metaplastic carcinoma in a 69-year-old female who had an extensive cystic change in radiologic and histopathologic findings. The precise cell type that gives rise to metaplastic carcinomas remains uncertain. Immunohistochemical findings raised the possibility of the myoepithelial nature of the tumor.
Aged ; Breast* ; Female ; Humans ; Immunohistochemistry ; Metaplasia

No abstract available.

This study was undertaken to observe the mutagenic occurrence in urine excreted after the ingestion of roast beef. Two healthy nonsmoker persons of both sex were selected for this test, employing two strains (TA98, TA100) of Salmonella typhimurium according to Ames' method. The mutagenic activity began to appear in urine of both sex three hours after ingestion of 300 g of roast beef, gradually increasing until 6 hours and declining thereafter.
Eating* ; Humans* ; Methods ; Red Meat* ; Salmonella typhimurium

We Performed an immunohistochemical study to identify the cellular components and involvement of immune mechanism in proliferative vitreoretinopathy, which is a major cause of delayed filure of retinla surgery. The 17 specimens of periretinal membranes -including vitreal membranes- were surgically obtained during the pars plana vitrectomy. The clinical diagnoses were idiopathic or traumatic retinal detachment (9 eyes), proliferative diabetic retinopathy (6 eyes), and pars plannitis (2 eyes). The labeled streptavidin-biotin immunoperoxidase method was used for immunohistochemical stain. The following antigens were detected in periretinal membranes : cytokeratin in 8 (of 17 cases studied for this antigen), glial fibrillary acidic protein (GFAP) in 9 (of 17), vimentin in 15 (of 17), HLA-DR in 14 (of 17). The macrophages and T lymphocyte expressing CD4 or CD8 markers, were not found in any of the membranes. These results suggest that cellular components of periretinal membranes are consists of retinal pigment epithelial cells, glial cells, and fibroblast. The identification of macrophage and T lymphocytes all met with failure. Also, strong positivity of HLA-DR antigen may indicate involvement of the immune mechanism during the course of proliferative vitreoretinopathy.
Diabetic Retinopathy ; Diagnosis ; Epithelial Cells ; Fibroblasts ; Glial Fibrillary Acidic Protein ; HLA-DR Antigens ; Keratins ; Lymphocytes ; Macrophages ; Membranes ; Neuroglia ; Retinal Detachment ; Retinaldehyde ; T-Lymphocytes ; Vimentin ; Vitrectomy ; Vitreoretinopathy, Proliferative

The patients of developmental dislocation of the hip(DDH) are almost found after walking age because of early diagnosis of DDH in younger children is not easy. A controversy still exists as to the relative value of closed and operative management in the treatment of a child who has reached walking age. This study is a report of the results of 16 patients(17 cases) in developmental dislocation of the hip who have visited our hospital at the age of 9 months to 3 years old, and have been followed more than 12 months (12-112 months) on review of plain radiographs and arthrograms. The results were as follows 1. The age at diagnosis was 16.4 months(9-31 months) in average. The methods of treatment were conservative for 8 cases, and operation for 4 cases and secondary operation for 5 cases who were failed with conservative therapy. 2. By Severin's radiologic grade, the result was good in 4 cases, fair in 3 cases and poor in 1 case in conservative treatment. In operative treatment, fair was in 2 cases and poor in 2 cases. In secondary operative -patients who were failed with conservative therapy, good was in 2 cases, fair in 2 cases and poor in 1 case. 3. Avascular necrosis of femoral head was developed in 3 cases. 4. In the good reduction as determined by arthrogram according to Race and Herring, we can get a favorable result in conservative treatment, and in the poor reduction as determined by arthrogram, the better
Child ; Continental Population Groups ; Diagnosis ; Dislocations* ; Early Diagnosis ; Head ; Hip* ; Humans ; Necrosis ; Walking*

To describe and evaluate the morphologic changes and the different expression of cell-specific or correlating protein molecules during cell growth, immunocytochemistry and morphologic observations were done on retinal pigment epithelial(RPE) cells obtained from several culture conditions. These include culture time, spatial or cell density, transdifferentiation, and presence of growth factors. The human fetal and porcine RPE cells were cultured with and without individual growth factor or in combinations inchlding extracellular matrix (ECM), Insulin, basic fibroblatio growth factor (bFGF) and epidermal growth factor (EGF). Mouse monoclonal anti-human, or anti-mouse antibodieg with or without species cross reactlvity against the intermediate filament proteins (cytokeratin, vimentin, GFAP) were used. To determine RPE-specific molecules of cytokeratin, nine commercially available antibodies, representing subclones of Moll's catalog number 1, 5, 7, 8, 10, 14, 17, 18, 19 were applied. The morphological changes and the proliferation of cells started after their attachment on the culture plate as soon as they lost pigment granules. The epithelial cells like fibroblasts occurred in the area where the cellular density was low, and finally, their shape was restored to their original phenotype when the cellular connuency was achieved. The degree of proliferation and the duration of achieving confluency of cells were dependent on whether ECM and growth factors were added in media or not. Cells with the epithelial morphology were positively stained with anticytokeratine antibodies, especially with clone 19, 18, 17, 8 and 7 in human RPE cells; with 19, CAM 5.26 (8/18) in porcine cells. The fusiform or digitating cells of sparse density also expressed vimentin strongly through out all stages, whereas GFAP was not expressed at any stage in either species.
Animals ; Antibodies ; Cell Count ; Clone Cells ; Epidermal Growth Factor ; Epithelial Cells* ; Extracellular Matrix ; Fibroblasts ; Humans ; Immunohistochemistry ; Insulin ; Intercellular Signaling Peptides and Proteins ; Intermediate Filament Proteins ; Keratins* ; Mice ; Phenotype ; Retinaldehyde* ; Vimentin*

To describe and evaluate the morphologic changes and the different expression of cell-specific or correlating protein molecules during cell growth, immunocytochemistry and morphologic observations were done on retinal pigment epithelial(RPE) cells obtained from several culture conditions. These include culture time, spatial or cell density, transdifferentiation, and presence of growth factors. The human fetal and porcine RPE cells were cultured with and without individual growth factor or in combinations inchlding extracellular matrix (ECM), Insulin, basic fibroblatio growth factor (bFGF) and epidermal growth factor (EGF). Mouse monoclonal anti-human, or anti-mouse antibodieg with or without species cross reactlvity against the intermediate filament proteins (cytokeratin, vimentin, GFAP) were used. To determine RPE-specific molecules of cytokeratin, nine commercially available antibodies, representing subclones of Moll's catalog number 1, 5, 7, 8, 10, 14, 17, 18, 19 were applied. The morphological changes and the proliferation of cells started after their attachment on the culture plate as soon as they lost pigment granules. The epithelial cells like fibroblasts occurred in the area where the cellular density was low, and finally, their shape was restored to their original phenotype when the cellular connuency was achieved. The degree of proliferation and the duration of achieving confluency of cells were dependent on whether ECM and growth factors were added in media or not. Cells with the epithelial morphology were positively stained with anticytokeratine antibodies, especially with clone 19, 18, 17, 8 and 7 in human RPE cells; with 19, CAM 5.26 (8/18) in porcine cells. The fusiform or digitating cells of sparse density also expressed vimentin strongly through out all stages, whereas GFAP was not expressed at any stage in either species.
Animals ; Antibodies ; Cell Count ; Clone Cells ; Epidermal Growth Factor ; Epithelial Cells* ; Extracellular Matrix ; Fibroblasts ; Humans ; Immunohistochemistry ; Insulin ; Intercellular Signaling Peptides and Proteins ; Intermediate Filament Proteins ; Keratins* ; Mice ; Phenotype ; Retinaldehyde* ; Vimentin*

No abstract available.
Horner Syndrome* ; Intervertebral Disc Displacement ; Spinal Cord Compression*