No abstract available.

No abstract available.
Ear Cartilage* ; Temporomandibular Joint Disc* ; Temporomandibular Joint* ; Transplants*

Allogeneic demineralized bone powder has been shown to support the osteogenesis in a variety of bone defect and extra-osseous sites. Previous several experiments have been carried out in animal models which have time-consuming and various problems. Therefore author designed some cytotoxicity tests by using of 5-diphenyl dimethyltetrazolium bromide(MTT) assay, neutral red(NR) assay, lactate dehydrogenase(LDH) activity test, sulforbodamine B (SRB) assay, and alkaline phosphatase(ALP) assay. And these method seemed to be a rapid, repeatable and believable reaction. To establish the osteogenesis capacity of allogeneic bone according to various bone preparation(defatting and demineralization), author studied the effect of allogeneic bone on well-established rat 3T3 fibroblasts and MC3TEl osteoblasts in culture to evaluate a cytotoxicity and examined the surface of allogeneic bone with scanning electron microscopy(SEM) for detecting the porositv. Defatting Demineralized Bone Powder(DDBP0 and Non-Defatting Demineralized Bone Powder(NDDBP) of particle size 212mum were prepared from rat calvarial bone and long bones. 1. Cell counts of rat 3T3 fibroblasts and MC3T3E1 osteoblasts were greater in DDBP enriched medium when compared with medium with NDDBP. Cell counts with DDBP were to different from controls. 2. Author found a little differences in cytotoxicity on 3T3 fibroblast and MC3T3E1 osteoblasts between DDBP and NDDBP with MTT assay, NR assay, LDH activity test, SRB assay, Generally, DDBP was represented less cytotoxicity than NDDBP. 3. ALP assay with MC3T3E1 osteoblasts in culture presented the less cytotoxicity and more crowding around the DDBP than NDDBP. With these findings, defatting procedure on making the demineralized bone powder have a merit for supporting osteogenesis. I should be suggested that cell culture with DDBP and NDDBP is a good method for studying induced osteogenesis.
Animals ; Cell Count ; Cell Culture Techniques ; Crowding ; Fibroblasts ; Lactic Acid ; Models, Animal ; Osteoblasts ; Osteogenesis* ; Particle Size ; Rats ; Transplants*

No abstract available.
Fistula* ; Hemorrhoids* ; Humans

No abstract available.
Colitis, Ulcerative* ; Mesalamine* ; Ulcer*

No abstract available.
Fistula*

The fact that therapeutic hypothermia (TH) has lowered intracranial pressure and protected brain in severe traumatic brain injury (TBI) is well known throughout past sources and experimental data. In this paper, the result of TH in TBI needs to be confirmed. The result of North American Brain Injury Study; Hypothermia (NAVIS-H) 1 and 2, Eurotherm3235, Japan trauma society study was reviewed throughout randomized controlled study which performed recently. The prognosis was not confirmed throughout TH in NAVIS-H1; however, there was statistical significance among the group of 45 years or less and below 35 degree in celcius which checked when he or she visited initially. Hence, NAVIS-H2 study was preceded. In patient who had surgically removed hematoma, the effects of TH were proved compared to diffuse brain damage in NAVIS-H2 study. This was found in the result of Japan neurotrauma data bank. Eurotherm study has been doing, which leads to collect many data later on. The TBI of TH makes them better prognosis in patients who had surgically removed hematoma and lowered initial body temperature. Later on, it is considered further study is necessary.
Body Temperature ; Brain ; Brain Injuries* ; Hematoma ; Humans ; Hypothermia* ; Intracranial Pressure ; Japan ; Prognosis

No abstract available.
Bleomycin* ; Fibroblasts* ; Fluorouracil* ; Humans*

No abstract available.
Schizophrenia*

No abstract available.
Lasers, Solid-State*