Hang-loose technique(HLT) is a reliable recession method in strabismus surgery. However, the loop length and the parallel of the anterior muscle border may not be sustained when the muscle was recessed behing the equator. This study was designed to assess the postoperative status of the large-recessed superior rectus muscle using the HLT and a modification of HLT(M-HLT) respectively in rabbits. Some of the recessed muscles with both techniques showed forward creeping. The average creeping was 0.5 mm on M-HLT and 1.1 mm on HLT in 7 mm recession group, and the difference was statistically significant(p=0.023). In 8 mm recession group, the average creeping was 1.0 mm on M-HLT and 1.8 mm on HLT. The difference was statistically significant(p=0.012). Although the parallel of the anterior muscle border was better on HLT than on M-HLT, the difference was not significant statistically. According to our result, M-HLT may be an useful alternative method in the cases of large recession with HLT.
Muscles ; Rabbits* ; Strabismus

We describe a case of xanthoma on the corneoscleral limbus in a 21-year-old man. It was characterized by a localized, round, elevated, yellowish mass on the right superonasal limbus with 6mm in size. There was no correlation with systemic or metabolic diseases such as hyperlipidemia and the microscopic examination revealed scanty fibrous element.
Humans ; Hyperlipidemias ; Metabolic Diseases ; Xanthomatosis* ; Young Adult

We describe a case of xanthoma on the corneoscleral limbus in a 21-year-old man. It was characterized by a localized, round, elevated, yellowish mass on the right superonasal limbus with 6mm in size. There was no correlation with systemic or metabolic diseases such as hyperlipidemia and the microscopic examination revealed scanty fibrous element.
Humans ; Hyperlipidemias ; Metabolic Diseases ; Xanthomatosis* ; Young Adult

Choroidal osteoma, a benign tumor and involving one eye frequently, occurred around optic disc in usually healthy young women, In our cases, the ophthalmoscopic examination revealed a sharply circumscribed, slightly elevated, orange-red subretinal lesion with irregular geographic borders and the tumors became depigmented and yellow-white with time. Diffuse and mottled depigmentation of the overlying pigment epithelium was occurred and the tufts of branching blood vessels were scattered on the tumor suface. In case 1, the tumor size was enlarged with pregnancy. In case 2, the tumor size was progressively enlarged with time in right eye and new lesion was developed in left eye, which had been normal at the first visit. The causes of visual disturbance were degeneration of retinal pigment epithelium and sensory retina in case 1 and right eye of case 2, and submacular neovascular membrane in left eye of case 2.
Blood Vessels ; Choroid* ; Epithelium ; Female ; Humans ; Membranes ; Osteoma* ; Pregnancy ; Retina ; Retinal Pigment Epithelium

The authors observed histologic changes after subconjunctival injection of steroid (triamcinolone acetonide; TA) around the operated superior rectus muscle to investigative the alteration of subconjunctival collagen and muscle fibers in rabbits. The TA was identified in the subconjunctival tissue as an eosinophilic, foamy deposit with infiltration of macrophages phagocytosing TA particles and a few inflammatory cells The deposit was surrounded by compact collagen fibers that formed a pseudocapsule. The collagen fibers and fibroblasts were altered and fibrolytic in appearance within and margin of the drug deposit. While there was only mild degeneration in the muscle and its surrounding connective tissue. And the collagen fibers and fibroblasts at the site distant from the TA deposit were normal. There was no difference in histologic findings between the resected and recessed muscles. From these observations, it would appear that TA has a local fibrolytic effect. The localized fibrolytic effect within and marign of the drug deposit may explain, in part, the fibrolytic action of macrophages, which contained phagocytosed TA.
Collagen ; Connective Tissue ; Eosinophils ; Fibroblasts ; Macrophages ; Muscles ; Rabbits

New Cervicography and HPV-DNA test, a adjunctive Pap Smear test, are an innovative cervical cancer surveillance system. The purpose of this study was to investigate the role of HPV-DNA test and cervicography as a pap smear in early detection of cervical cancer. Pap smear, cervicography, and HPV-DNA test data were obtained from 161 patients who visited the Department of Obstetrics and Gynecology, Chunan Hospital, Soonchunhyang University from November 1997 to April 1998. Histologic specimens were obtained from patients in whom abnormalities were detected by either pap smear or cervicogram, and by naked eye. Specimens were taken either by colposcopically directed biopsy or large loop excision of the transformation zone. Results were as follows: 1. Pap smear results were normal in 40 cases (24.8%), RCC (reactive cellular change) or ASCUS (atypical squamous cells of undetermined significance) in 74 cases (46%), and abnormal (above low grade squamous intraepithelial lesion) in 47 cases (29.2%). 2. New Cervicographic findings were negative in 93 cases (57.8%), benign or suspicious atypical in 14 cases (8.7%), and positive in 54 cases (33.5%). 3. The sensitivity (94.6% vs 67.7%, p<0.01), and the false positive rate (19.8% vs 14.6%, p<0.01) of cervicography were significantly higher than for pap smear. The specificity (83.0% vs 86.2%, p<0.01), and the false negative rate (5.4% vs 32.3%, p<0.01) of cervicography were significantly lower than for pap smear. 4. When New Cervicography and Pap smear were used together, the sensitivity was higher than for pap smear in New Cervicography used alone (p<0.01) and the specificity was lower than for pap smear or cervicography used alone (p<0.01). 5. When cervicography and pap smear and HPV-DNA test were used concurrently, the sensitivity was higher than for cervicography and pap smear used together (p<0.01), and the specificity was lower than for cervicography and pap smear used together (p<0.01). The three screening test combination is a useful interval screening method to detect cervical cancer. The detection rate of cervical cancer will be increased. Thus, we believe that cervicography and HPV testing can be important adjuntive tests for cervical cytology, final tool in precancerous cervical lesions prevention. Combination of these three tests is sensitive enough to institute "interval screening" into society.
Biopsy ; Chungcheongnam-do ; Gynecology ; Humans* ; Mass Screening* ; Obstetrics ; Papilloma* ; Sensitivity and Specificity ; Uterine Cervical Neoplasms

New Cervicography and HPV-DNA test, a adjunctive Pap Smear test, are an innovative cervical cancer surveillance system. The purpose of this study was to investigate the role of HPV-DNA test and cervicography as a pap smear in early detection of cervical cancer. Pap smear, cervicography, and HPV-DNA test data were obtained from 161 patients who visited the Department of Obstetrics and Gynecology, Chunan Hospital, Soonchunhyang University from November 1997 to April 1998. Histologic specimens were obtained from patients in whom abnormalities were detected by either pap smear or cervicogram, and by naked eye. Specimens were taken either by colposcopically directed biopsy or large loop excision of the transformation zone. Results were as follows: 1. Pap smear results were normal in 40 cases (24.8%), RCC (reactive cellular change) or ASCUS (atypical squamous cells of undetermined significance) in 74 cases (46%), and abnormal (above low grade squamous intraepithelial lesion) in 47 cases (29.2%). 2. New Cervicographic findings were negative in 93 cases (57.8%), benign or suspicious atypical in 14 cases (8.7%), and positive in 54 cases (33.5%). 3. The sensitivity (94.6% vs 67.7%, p<0.01), and the false positive rate (19.8% vs 14.6%, p<0.01) of cervicography were significantly higher than for pap smear. The specificity (83.0% vs 86.2%, p<0.01), and the false negative rate (5.4% vs 32.3%, p<0.01) of cervicography were significantly lower than for pap smear. 4. When New Cervicography and Pap smear were used together, the sensitivity was higher than for pap smear in New Cervicography used alone (p<0.01) and the specificity was lower than for pap smear or cervicography used alone (p<0.01). 5. When cervicography and pap smear and HPV-DNA test were used concurrently, the sensitivity was higher than for cervicography and pap smear used together (p<0.01), and the specificity was lower than for cervicography and pap smear used together (p<0.01). The three screening test combination is a useful interval screening method to detect cervical cancer. The detection rate of cervical cancer will be increased. Thus, we believe that cervicography and HPV testing can be important adjuntive tests for cervical cytology, final tool in precancerous cervical lesions prevention. Combination of these three tests is sensitive enough to institute "interval screening" into society.
Biopsy ; Chungcheongnam-do ; Gynecology ; Humans* ; Mass Screening* ; Obstetrics ; Papilloma* ; Sensitivity and Specificity ; Uterine Cervical Neoplasms

BACKGROUND: Dynorphin A (1-17) is conceived as an endogenous opioid peptide with a high degree of selectivity forkappa- opioid receptor even though it has been reported to sometimes act like amicro- opioid agonist. The aim of this study was to investigate [35S] GTPgammaS binding stimulated activation by dynorphin A (1-17) in the cerebral and thalamic membranes of a rhesus monkey. METHODS: The rhesus monkey (Macaca mulatta, male, n = 1) was euthanized for the preparation of the cerebral and thalamic membranes. Protein concentrations were determined by the Bradford method. In the dynorphin A (1-17)-stimulated [35S] GTPgammaS binding dose-response curve, EC50 (effective concentration 50 nM) and maximum stimulation (% over basal) were determined in the absence or presence of themicro-andkappa-opioid receptor antagonists naloxone (20 nM) and norbinaltorphimine (nor-BNI, 3 nM), respectively. E2078-stimulated [35S] GTPgammaS binding was also determined in the absence or presence ofmicro-andkappa-opioid receptor antagonists in the cortical membrane and compared with dynorphin A (1-17). RESULTS: Values of EC50 and maximum stimulation of dynorphin A (1-17)-stimulated [35S] GTPgammaS binding were as follows: cortex (474 nM/32.0%) and thalamus (423 nM/45.3%). Nor-BNI (3 nM) did not antagonize dynorphin A (1-17)-stimulated [35S] GTPgammaS binding at all in cortical or thalamic membrane, but naloxone (20 nM) produced a 12.2 fold rightward shift of the dynorphin A (1-17)-stimulated [35S] GTPgammaS binding dose-response curve in the thalamic membrane. The EC50 and the maximum stimulation of E2078-stimulated [35S] GTPgammaS binding were 65.6 nM and 22.7%, respectively. In E2078-stimulated [35S] GTPgammaS binding, the dose-response curve was antagonized not by nor-BNI but by naloxone but in the cortical membrane (a 14.2 times rightward shift). CONCLUSIONS: Dynorphin A (1-17) is selective formicro-opioid receptor in agonist-stimulated [35S] GTPgammaS binding in the cortical and thalamic membranes of rhesus monkey.
Dynorphins* ; Guanosine 5'-O-(3-Thiotriphosphate)* ; Haplorhini* ; Humans ; Macaca mulatta ; Male ; Membranes* ; Naloxone ; Opioid Peptides ; Receptors, Opioid ; Thalamus

Widespread brain-derived neurotrophic factor (BDNF) mRNA expression has been detected in the region of catecholamine groups of the rat lower brainstem, while few BDNF-immunoreactive cells were found in this area. In the present study, a double-color immunofluorescence (IF) technique for BDNF and tyrosine hydroxylase (TH) after colchicine treatment was employed to evaluate the possible presence of BDNF immunoreactivity in the catecholamin-ergic cells of rat lower brainstem. Additionally, a double-color IF technique for BNDF and TH and in situ hybridiza-tion for BDNF mRNA were performed to see effects of hemorrhage on the expression of BDNF and its mRNA. We detected many new BDNF-immunoreactive cells in the A1, A2, A4, A6-A10 and C1-C3 cell groups and in the other lower brainstem nuclei where, without colchicine treatment, BDNF mRNA was expressed, but not BDNF immunoreactivity. In addition, the catecholaminergic neurons were found to express BDNF immunoreactivity with the co-existence being greatest, in percentage terms, in medullary catecholaminergic cell groups. Hypotensive hemorrhage, which activates medullary catecholaminergic neurons, induced the expression of BDNF immunoreactivity in catecholaminergic neurons (A1/C1 and C2) and increased the number of BDNF mRNA-containing neurons in the area. These results demonstrate that BDNF is regulated by activity in medullary catecholaminergic cell groups involved in central cardiovascular regulation.
Animals ; Brain Stem* ; Brain-Derived Neurotrophic Factor* ; Colchicine ; Fluorescent Antibody Technique ; Hemorrhage* ; In Situ Hybridization ; Neurons* ; Rats* ; RNA, Messenger* ; Tyrosine 3-Monooxygenase

PURPOSE: To evaluate the frequency and types of chromosome aberrations induced by ionizing radiation in cancer patients before and after radiotherapy. MATERIALS AND METHODS: Thirty cases with various types of cancers such as rectum, lung, uterine cervix, lymphoma, and etc. were studied. The time of analyses was within 1 month after radiotherapy. The mean total radiation dose was 54 Gy. RESULTS: The incidence of aberrant metaphases prior to radiation treatment was 2.2% and increased significantly after treatment to 29.7% (p<0.001). Also the number of chromosomal aberrations per cell was increased by a factor of 38.5. A comparison of chromosome abnormalities observed before and after treatment indicated that dicentrics, acentrics, derivatives and translocations were increased by a factor of 155.5, 99, 55.5 and 23.8, respectively, after radiation treatment. Breakages were located at all chromosomes except Y chromosome and total number of identifiable breakages was 426 in 900 observed lymphocyte metaphases. Distribution of break points revealed the increase of number of break points in chromosome 1,2,5 and 11q. CONCLUSION: Ionizing radiation produces more asymmetrical than symmetrical chromosome aberrations and more two-break than one-break anomalies in radiation-treated cancer patients.
Cervix Uteri ; Chromosome Aberrations ; Cytogenetics* ; Female ; Humans ; Incidence ; Lung ; Lymphocytes* ; Lymphoma ; Metaphase ; Radiation, Ionizing ; Radiotherapy* ; Rectum ; Y Chromosome