No abstract available.
Burns* ; Granulation Tissue*

No abstract available.
Chondrogenesis* ; Ear*

PURPOSE: The intermediate filament proteins, desmin and vimentin, are specific components of the cytoskeleton of striated muscle fibers and of mononuclear cells of mesenchymal origin including myoblasts, respectively. Desmin has also been found in presumptive myoblasts of mammals. The aim of this experiment was attempted to observe the phenotypic changes of intermediate filaments in skeletal muscle fibers during early stages of sciatic nerve crushing injury. MATERIALS AND METHODS: The sciatic nerves of rats were surgically crushed by hemostat and serial cryosections of soleus and extensor digitorum longus(EDL) muscles were prepared at 2, 4, 6, 8, 10, 15, 20 and 27 days after nerve injury. Serial cryosections were immunolabelled with desmin, vimentin and laminin and were histochemically reacted with NADH-TR. RESULTS: 1) Firstly, desmin positive fibers were appeared in fast-twitch type C fibers of both muscles at 6 days after nerve crushing, but were not reacted for vimentin. 2) Co-expressions of desmin and vimentin were firstly detected in fast-twitch type A fibers of EDL muscles at 8 days after nerve injury. In soleus muscles, co-expressions of desmin and vimentin were firstly seen in slow-twitch type B fibers at 10 days after nerve injury. Many atrophic fibers, that contained several central nuclei like myotubes and co-expressed desmin and vimentin, were appeared in EDL muscles at 10 days after nerve injury. Although whole regions of fibers were regenerated in EDL muscles, only peripheral regions of fibers were regenerated in soleus muscles at 15 days after nerve injury. Many atrophic fibers, co-expressed of desmin and vimentin, were appeared in EDL muscles at 20 days after nerve injury. These whole fibers represented various degrees of regenerating stages. Most of mature fibers containing several central nuclei, only expressed vimentin slightly, were seen in soleus muscles at 20 days after nerve injury. Most fibers of both muscles were matured at 27 days after nerve injury, but some fibers in EDL muscles were still in processing of degeneration and regeneration. No expressions of desmin and vimentin indicated that muscle fibers were almostly matured in soleus muscles at 27 days after nerve injury. 3) Targetoid or target fibers which informed reinnervation, were appeared firstly in soleus muscles at 20 days and were seen in both muscles at 27 days after nerve injury. All targetoid and target fibers were type B fibers. CONCLUSION: Desmin was revealed in processes of degeneration and regeneration and vimentin was appealed in regeneration process. At the same time, positive immunoreactivity of desmin and vimentin showed specific differences in degree of degeneration and regeneration according to different muscles and muscle fibers.
Animals ; Cytoskeleton ; Desmin ; Intermediate Filament Proteins* ; Intermediate Filaments* ; Laminin ; Leg* ; Mammals ; Muscle Fibers, Skeletal ; Muscle, Striated ; Muscles* ; Myoblasts ; Nerve Crush ; Nerve Fibers, Myelinated ; Nerve Fibers, Unmyelinated ; Rats* ; Regeneration* ; Sciatic Nerve* ; Vimentin

PURPOSE: The intermediate filament proteins, desmin and vimentin, are specific components of the cytoskeleton of striated muscle fibers and of mononuclear cells of mesenchymal origin including myoblasts, respectively. Desmin has also been found in presumptive myoblasts of mammals. The aim of this experiment was attempted to observe the phenotypic changes of intermediate filaments in skeletal muscle fibers during early stages of sciatic nerve crushing injury. MATERIALS AND METHODS: The sciatic nerves of rats were surgically crushed by hemostat and serial cryosections of soleus and extensor digitorum longus(EDL) muscles were prepared at 2, 4, 6, 8, 10, 15, 20 and 27 days after nerve injury. Serial cryosections were immunolabelled with desmin, vimentin and laminin and were histochemically reacted with NADH-TR. RESULTS: 1) Firstly, desmin positive fibers were appeared in fast-twitch type C fibers of both muscles at 6 days after nerve crushing, but were not reacted for vimentin. 2) Co-expressions of desmin and vimentin were firstly detected in fast-twitch type A fibers of EDL muscles at 8 days after nerve injury. In soleus muscles, co-expressions of desmin and vimentin were firstly seen in slow-twitch type B fibers at 10 days after nerve injury. Many atrophic fibers, that contained several central nuclei like myotubes and co-expressed desmin and vimentin, were appeared in EDL muscles at 10 days after nerve injury. Although whole regions of fibers were regenerated in EDL muscles, only peripheral regions of fibers were regenerated in soleus muscles at 15 days after nerve injury. Many atrophic fibers, co-expressed of desmin and vimentin, were appeared in EDL muscles at 20 days after nerve injury. These whole fibers represented various degrees of regenerating stages. Most of mature fibers containing several central nuclei, only expressed vimentin slightly, were seen in soleus muscles at 20 days after nerve injury. Most fibers of both muscles were matured at 27 days after nerve injury, but some fibers in EDL muscles were still in processing of degeneration and regeneration. No expressions of desmin and vimentin indicated that muscle fibers were almostly matured in soleus muscles at 27 days after nerve injury. 3) Targetoid or target fibers which informed reinnervation, were appeared firstly in soleus muscles at 20 days and were seen in both muscles at 27 days after nerve injury. All targetoid and target fibers were type B fibers. CONCLUSION: Desmin was revealed in processes of degeneration and regeneration and vimentin was appealed in regeneration process. At the same time, positive immunoreactivity of desmin and vimentin showed specific differences in degree of degeneration and regeneration according to different muscles and muscle fibers.
Animals ; Cytoskeleton ; Desmin ; Intermediate Filament Proteins* ; Intermediate Filaments* ; Laminin ; Leg* ; Mammals ; Muscle Fibers, Skeletal ; Muscle, Striated ; Muscles* ; Myoblasts ; Nerve Crush ; Nerve Fibers, Myelinated ; Nerve Fibers, Unmyelinated ; Rats* ; Regeneration* ; Sciatic Nerve* ; Vimentin

PURPOSE: Chronic bacterial prostatitis (CBP) is the most common urological disease in adult males, with antibiotic therapy being the gold standard for its treatment. However, long-term therapy results in many side effects as well as bacterial resistance. For these reasons, there is a need for a new treatment modality to replace traditional antibiotic therapy. Lycopene, an extract of tomatoes, has antioxidant effects against various bacteria and synergistic effects with antibiotics. We evaluate the synergistic effects of lycopene on the treatment of CBP in an animal model. MATERIALS AND METHODS: Forty five rats demonstrating CBP were randomly divided into 4 groups; the control, lycopene, ciprofloxacin and lycopene with ciprofloxacin groups. All drug treatments were conducted over a period of 2 weeks. After treatment, the results were analyzed, with the microbiological cultures and histological findings of the prostate and urine samples compared with the control group and between each group. RESULTS: The uses of ciprofloxacin, and lycopene with ciprofloxacin showed statistically significant decreases in bacterial growth and improvements in prostatic inflammation compared with the control group (p<0.05). The lycopene with ciprofloxacin group showed a statistically significant decrease in bacterial growth and improvements in prostatic inflammation compared with the ciprofloxacin group (p<0.05). CONCLUSIONS: These results suggest that lycopene may be an effective material in the treatment of CBP. Especially, the combination treatment of lycopene and ciprofloxacin has synergistic effects. Therefore, it is suggest that the combination of lycopene and ciprofloxacin may be effective in the treatment of CBP, and with a higher success rate.
Adult ; Animals ; Anti-Bacterial Agents ; Antioxidants ; Bacteria ; Ciprofloxacin ; Humans ; Inflammation ; Lycopersicon esculentum ; Male ; Models, Animal* ; Prostate ; Prostatitis* ; Rats* ; Urologic Diseases

Invasive aspergillosis is a life-threatening infectious disease in immunocompromised patients. Prolonged neutropenia is the most important predisposing factor. Bronchial casts are generally associated with pneumonia, bronchiectasis, asthma, chronic bronchitis, allergic bronchopulmonary aspergillosis and mucoviscidosis. We experienced.a case of invasive aspergillosis characterized by the formation of large bronchial cast in patient with acute myelogenous leukemia(AML) during induction chemotherapy. The patient expectorated a large bronchial cast without airway obstruction. Aspergillus was disclosed in intraluminal surface of bronchial cast. The patient's conditions improved after amphotericin B therapy and expectoration of a large bronchial cast.
Airway Obstruction ; Amphotericin B ; Aspergillosis* ; Aspergillosis, Allergic Bronchopulmonary ; Aspergillus ; Asthma ; Bronchiectasis ; Bronchitis, Chronic ; Causality ; Communicable Diseases ; Cystic Fibrosis ; Humans ; Immunocompromised Host ; Induction Chemotherapy ; Leukemia, Myeloid, Acute* ; Neutropenia ; Pneumonia

PURPOSE: This prospective study was conducted to evaulate the effect of nebulized steroid (budesonide) and parenteral steroid (dexamethasone) on chidren with croup. METHODS: Twenty nine infants and children (6months-5years of age) admitted to hospital with croup were randomly assigned to receive either 1mg (4ml) of nebulized budesonide (17 patients) or 0.6mg/kg intramuscular injection of dexamethasone sodium phosphate (12 patients). Total croup score, heart rate and respiratory rate of patients were assessed on admission, 30 minutes, 2 hours, 6 hours, 12 hours and 24 hours after treatment, respectively. RESULTS: 1) In total cases, total croup score were significantly decreased at 30 minutes after treatment in the group treated with nebulized budesonide (p<0.01) but at 2 hours after treatmetn in the group treated with dexamethasone (p<0.05). 2) In mild cases (total croup score<5), total croup score were significantly decreased at 2 hours after treatment in the both group (p<0.05). 3) In moderate to severe cases (total croup score> or =5), total croup score were significantly decreased at 30 minutes after treatment in the group treated with nebulized budesonide (p<0.05). But in group treated with dexamethasone, there were no statistical significance because of small cases. 4) Respiratory rate was decreased after treatment in the both group, but significantly decreased at 2 hours after treatment in the group treated with dexamethasone (p<0.05). 5) Heart rate was decreased after treatment in the both group, but significantly decreased at 30 minutes after treatment in the group treated with dexamethasone (p<0.05). 6) There were no significant side effects in the both group. CONCLUSIONS: We conclude that both nebulized budesonide and parenteral dexamethasone were effective in treatment of croup, and that nebulized budesonide leads to more rapid clinical improvement in children with croup, especially moderate to severe one.
Budesonide ; Child* ; Croup* ; Dexamethasone ; Heart Rate ; Humans ; Infant ; Injections, Intramuscular ; Prospective Studies ; Respiratory Rate ; Sodium

Evans syndrome refer to autoimmune hemolytic anemia accompanied by thrombocytopenia. The etiologic factors of Evans syndrome are unknown but viral infections such as Epstein-Barr virus and cytomegalovirus are suggested. Epstein-Barr virus (EBV) infection is an acute illness characterized clinically by fever, sore throat, and lymphadenopathy; serologically by the transient positive heterophil antibody; and hematologically by mononuclear leukocytosis with atypical lymphocytosis. We experienced a case of Epstein-Barr virus infection presented as Evans syndrome. Epstein-Barr virus infection was diagnosed by positive heterophil antibody and EBV viral capsid antigen IgM. Autoimmune hemolytic anemia and immune thrombocytopenic purpura were completely resolved by steroid therapy.
Anemia, Hemolytic, Autoimmune ; Capsid ; Cytomegalovirus ; Fever ; Herpesvirus 4, Human* ; Immunoglobulin M ; Leukocytosis ; Lymphatic Diseases ; Lymphocytosis ; Pharyngitis ; Purpura, Thrombocytopenic, Idiopathic ; Thrombocytopenia

Invasive aspergillosis is a life-threatening infectious disease in immunocompromised patients. Aspergillus is an ubiquitous mold present as normal flora in paranasal sinus, nose, skin and lung. The most important determinant of infection is the immune status of the patient, not the intensity of exposure. In acute leukemia and bone marrow transplantation, prolonged neutropenia is probably the most important predisposing factor. We experienced a case of invasive aspergillosis involving lung and brain in patient with acute leukemia during remission-induction chemotherapy. Invasive aspergillosis involving lung and brain was diagnosed by sputum culture, computed tomography (CT) guided lung biopsy and brain magnetic resonance imaging (MRI). Early diagnosis and prompt treatment for invasive aspergillosis are essential for lowering mortality in immunocompromised patients.
Aspergillosis* ; Aspergillus ; Biopsy ; Bone Marrow Transplantation ; Brain* ; Causality ; Communicable Diseases ; Drug Therapy ; Early Diagnosis ; Fungi ; Humans ; Immunocompromised Host ; Leukemia* ; Lung* ; Magnetic Resonance Imaging ; Mortality ; Neutropenia ; Nose ; Skin ; Sputum

Primary lymphoma of the central nervous system is a rare disease, occurring spontaneously and/or in conjunction with immunosuppressive state. Its incidence is increasing according to the increment of organ transplantation and AIDS. Recently we experienced a case of primary lymphoma occurred in central nervous system after renal transplantation in a 58-year-old women who had complained of persistent headache and left hemiparesis. CT scan of the brain showed two hyperdense mass lesions in right frontal and right basal ganglia areas. Immunohistochemical stain of the excised mass lesion revealed that tumor cells were derived from B cells. The patient was treated with discontinuance of immunosuppressive drug and irradiation, but expired due to pneumonia.
B-Lymphocytes* ; Basal Ganglia ; Brain ; Central Nervous System ; Female ; Headache ; Humans ; Immunosuppression ; Incidence ; Kidney Transplantation* ; Lymphoma ; Lymphoma, B-Cell* ; Middle Aged ; Nervous System* ; Organ Transplantation ; Paresis ; Pneumonia ; Rare Diseases ; Tomography, X-Ray Computed ; Transplants