Objective:To construct eukaryotic expression vectors pEGFP-N2/MafA and pEGFP-C1/MafA,encoding the mouse musculus v-maf musculoaponeurotic fibrosarcoma oncogene family,protein A(MafA)gene,for the further research of its expression in HepG-2 cells.Methods:pRNA of the mouse MafA gene was distilled by RT-PCR from the mouse,and inserted into Hind Ⅲand SalⅠrestriction sites by PCR,then cloned into pEGFP-N2 and pEGFP-C1 vectors to obtain plasmids pEGFP-N2/MafA and pEGFP-C1/MafA.Human liver cancer cells(HepG-2) were transfected with formed plasmids by means of lipidosome.The MafA-EGFP fused protein was viewed directly with fluorensce microscope,and expression of MafA and insulin Ⅱ was detected by RT-PCR.Results:The mouse MafA gene was amplified through RT-PCR and successfully cloned into transfer vectors.The favorite gene sequence could be expressed and was completely consistent with that reported in genebank,but the insulin Ⅱ gene expression was not detected.Conclusion:The recombinant plasmids pEGFP-N2/MafA and pEGFP-C1/MafA were successfully constructed.But the insulin Ⅱgene was not expressed.

Colorimetry ; methods ; Humans ; Manganese ; urine ; Potassium Permanganate

Objective To evaluate the pharmacokineties of ropivacaine for single sciatic nerve block in dogs.Methods Twelve healthy adult mongrel dogs weighing 14-17 kg were randomized to receive 0.5% ropivaeaine 10 mg/kg or 20 mg/kg for single sciatic nerve block(n=6 each).ECG,BP and HR were monitored and recorded during anesthesia.Blood samples were obtained from femoral artery before ropivacaine injection (baseline)and at 10,20,30,40,60,90,120,150,180,240,360 and 720 min after ropivacaine injeetion for determination of plasma ropivaeaine concentration(by reversc-phasc high performance liquid chromatography).Arterial blood samples were also taken when adverse reactions occurred.The pharmacokinetic parameters were eMeulated with DAS 1.0 software package.Results The concentration-time curve of ropivacaine for single sciatic nenre block was fitted to two-compartment open model in both groups.The peak plasma concentration of ropivaeaine was significantly lower in 10 mg/kg group than in 20 mg/kg group.Two dogs developed convulsion in 20 mg/kg group.The plasma ropivaeaine concentration was 12.56 and 13.67 mg/L respectively during convulsion.Conclusion Pharmaeokinetic profile of ropivaeaine for single sciatic nerve block is best described by two-compartment model.Bopivaeaine 20 mg/kg for sciatic nerve block can hardly be tolerated by dogs.

From ancient to present,liver has always been a mystery and magical organ.As the largest glandular organ of the body,liver has many vital functions.Its regenerative capabilities and functional reservation allow major resection of up to 80%of its volume.Many diseases,such as liver cancer,cirrhosis and hepatolithiasis Can affect liver,and the mainstay of curative treatment is liver resection.Technological advances play an important role in modern surgery,and a new surgical paradigm characterized by precision has evolved.Recently,we haveadvocated the concept of"precise hepatectomy"on the basis of modem anatomy,physiology,oncology,imaging and surgical techniques,which is characterized by minimal invasion,liversaving and maximally restoration of liver function.Related subjects,including computer sciences,imaging and information are needed to be combined to create a new alliance of treatment.Although,there are still many theoretical and technical problems about liver resection and liver function preservation.With the development of science and technology,precise hepatectomy will further improve the outcomes of liver surgery.

In the medical field, the innovation education is to cultivate residents in China, which is an important way to senior medical elite. By analyzing competency-based neurosurgery residency training mode, the training methods for innovative ability of competency-based neurosurgeon are further discussed, for the purpose of reforming the training mode of neurosurgeon, improving creative ability and scientific clinical thinking ability.

Objective To discuss the treatment value of Jinxiangdan tablets on the pre-thrombus state of patient with coronary heart disease and angina pectoris. Methods 166 cases of clinical diagnosed coronary heart disease and angina pectoris were randomly divided into the treatment group and the control group. The changes of Fg, vWF, P-selectin, D-dimer, t-PA, ET-1 of two groups before and after the treatment were observed. Results Before the treatment, Fg, vWF, P-selectin, D-dimer and ET-1 elevate, while t-PA reduces. After the treatment of Jinxiangdan, Fg, vWF, P-selectin, D-dimer and ET-1 were decreased, t-pA were increased. Conclusion Jinxiangdan tablets can improve the hemoglutination fibrinloytic system and secretion function of endothelial cell, reduce the activity of platelet, to prevent the cardiovascular events.

OBJECTIVE:To evaluate the permeation enhancing effect of Flos Caryophylli volatile oil versus azone on diclofenac sodium.METHODS:Percutaneous absorption experiment was performed on the apparatus of isolated skin,and the penetrated amounts of diclofenac sodium at different time were measured,then the steady-state flux and enhancing rate were calculated,and the deposit effect of diclofenac sodium was investigated.RESULTS:Flos Caryophylli volatile oil and azone all had promotion effect on percutaneous absorption of diclofenac sodium,especially when they used in combination.Used either in combination or alone,they all had remarkable enhancement property on deposit effect of diclofenac sodium(P

OBJECTIVE: To discuss the focus of the legislative protective of Chinese traditional medicine. METHODS: The key elements in the legislative protective of Chinese traditional medicine were analyzed. RESULTS & CONCLUSION: Chinese traditional medicine literature, Chinese herbal medicine resources and medicinal genetic resources should be considered as the key elements in the legislative protective of Chinese traditional medicine.

After first report of local anesthetics on cardiac toxicity was published in 1979, A lot of researches of local anesthetics on the mechanism of cardiac toxicity and its treatments were reported, and some advances were reviewed in this article during recent years.

Objective To compare the medium-term clinical effects of oblique T-shaped volar locking compression plate (LCP) and external fixation (EF) treating type C distal radial fractures.Methods Data of 60 patients with type C distal radial fractures who underwent surgery in our hospital from May 2010 to November 2012 were retrospectively analyzed.36 patients received open reduction with LCP fixation (LCP group),among which there were 21 males and 15 females,and there were 20 left wrists and 16 right wrists with an average age of 45.94±16.29 years old (range,17-80 years old).24 patients received close reduction and external fixation (EF group),among which there were 16 males and 8 females,and there were 15 left wrists and 9 right wrists with an average age of 43.63±14.55 years old (range,16-79 years old).The clinical results of wrist joint were assessed by range of wrist activity,VAS,Gartland-Werley wrist score,postoperative radial tilt angle,radial inclination angle and radial height.Results All the 60 patients were followed up successfully.The LCP group was followed up for 24 to 55 (42.03 ± 8.62) months.The EF group was followed up for 24 to 54 (37.24±9.77) months.In the LCP group,the average VAS score was 1.28±0.78,Gartland-Werley score 2.28±0.78,wrist flexion angle 50.89°±5.53° and wrist dorsiflexion angle 50.69°±5.38°.In the EF group,the average VAS score was 1.38±0.71,Gartland-Werley score 2.92± 1.69,wrist flexion angle 50.96°±5.01° and wrist dorsiflexion angle 51.08°± 5.73°.No statistically differences were found in wrist joint function between two groups.In LCP group,radiological evaluation 2 years after operation showed that the average radial tilt angle was 10.69°±2.29,radial inclination angle 22.53°± 1.95° and radial height 10.63±1.14 mm.In the EF group,radial tilt angle was 6.63°±2.76°,radial inclination angle 16.96°±4.41° and radial height 9.06±0.98 mm.Significant differences were found in radial tilt angle,radial inclination angle and radial height.No statistically differences were found in postoperative complication rates.Conclusion Locking compression plate (LCP) fixation and external fixation (EF) can obtain similar satisfactory medium-term clinical outcome in treating type C distal radial fractures.Comparison with EF,LCP can achieve anatomic reduction under direct vision,and its radiological assessments including radial tilt angle,radial inclination angle and radial height were significantly better than external fixation.