OBJECTIVE To reinforce the personnel′s protection in supply division, to reduce the harmfulness of profession. METHODS The reasons of education, standard operation, dedicated supplies for protection, and building up wholesome institution were carried out. RESULTS The personnel′s consciousness of protection was reinforced and the arrangement of protection was carried out. Incidence wasn′t happened. CONCLUSIONS Better precaution and professional competency of personnel are the bases of protection for high quality work in supply division.

Bone Marrow Cells ; cytology ; physiology ; Humans ; Immunity ; Mesenchymal Stromal Cells ; cytology ; physiology

Child ; Complement C3 ; metabolism ; Cytokinins ; analysis ; metabolism ; Female ; Humans ; Immunoglobulin A ; immunology ; Immunoglobulin G ; immunology ; Interferon-gamma ; immunology ; Interleukin-12 ; analysis ; immunology ; Interleukin-2 ; analysis ; immunology ; Male ; Peptide Fragments ; immunology ; Respiratory Tract Infections ; epidemiology ; immunology ; virology ; Secondary Prevention ; Tuberculin ; analysis

Objective To investigate the effect of music electroacupuncture on perifocal ferric ions and neuronal apoptosis and the brain water content in rats with acute cerebral hemorrhage and explore the mechanism of its action on acute cerebral hemorrhage.Methods One hundred and twenty-eight healthy adult male Wister rats were randomized to normal, model, electroacupuncture and music electroacupuncture groups. In each group, four time points of 6 hrs, 24 hrs, 3 days and 7 days were set up, eight rats each time point. A rat model of acute cerebral hemorrhage was made using collagenase. In the electroacupuncture and music electroacupuncture groups, point Baihui was connected to the anode and point Taiyang to the cathode, 2 voltage was used as a selection parameter and electroacupuncture lasted 30 min. Intracerebral perifocal ferric ions and neuronal apoptosis and the brain water content were measured at four time points of 6 hrs, 24 hrs, 3 days and 7 days after model making.Results Intracerebral ferric ion content was higher in the model, electroacupuncture and music electroacupuncture groups of rats than in the normal group at 6 hrsafter model making (P<0.05), lower in the electroacupuncture and music electroacupuncture groups than in the model group at 24 hrs, 3 and 7 days after model making (P<0.05) and lower in the music electroacupuncture group than in the electroacupuncture group at 3 and 7 days after model making (P<0.05). Intracerebral neuronal apoptosis was higher in the model, electroacupuncture and music electroacupuncture groups of rats than in the normal group at 6 hrs after model making (P<0.05) and lower in the music electroacupuncture group than in the electro- acupuncture group at 7 days after model making (P<0.05). The brain water content was higher in the model, electro- cupuncture and music electroacupuncture groups of rats than in the normal group at 6 and 24 hrs after model making (P<0.05), lower in the electroacupuncture and music electroacupuncture groups than in the model group at 3 days after model making (P<0.05) and lower in the music electroacupuncture group than in the electroacupuncture group at 7 days after model making (P<0.05).Conclusions Music electroacupuncture has a benign regulating effect on intracerebral perifocal ferric ions, inhibits neuronal apoptosis around hematoma in the early stage and reduces cerebral edema in rats with acute cerebral hemorrhage. Its therapeutic effect is superior to that of electroacupuncture.

Objective To study the application of total intravenous anesthesia(TIVA) in patients with larp-aroscopic cholecystectomy(LC). Methods 100 cases with LC were randomly and evenly assigned to two groups: the combined intravenous with inhalation anesthesia(CIIA) group(n = 50) and the TIVA group(n = 50). The maintain anesthesia. BP, HR and SpO2 at all stages and time for extubation,the consciousness were recorded. Results The pneumoperitoneum after 10 min HR(96.8±13.5)times/min,SBP(142.5±14.3) mmHg、DBP(93.0±14.3) mm-Hg in A group higher than before induction of anesthesia(82.3±12.5) times/min, (129.0±21.7) mmHg, (77.3±13.5) mmHg(P < 0.05～0.01) ; The pneumoperitoneum after 10 min HR (84.0±11.0) times/min, pneumoperito-neum comphte(76.3±9.0) times/rain in B group lower than the(96.8±13.5) times/min, (84.1±11.0) times/min in A group(P >0.05); B group patients completed the opening time(6.5±1.5) min、extubation time (11.5±1.4) min、pestoperative nausea and vomiting in 3 cases,after 8 h VAS value of (2.8±1.1) in group B were lower than the (10.5±2.8) min,(25.2±9.5)min,12 cases(5.4±2.1) in group A(t =2. 411, P <0.05). Conclusion The application of TIVA to LC anesthetic effect is satisfaction.

Objective To observe the analgesic effect after cesarean section with different paregoric meth-ods. Methods 100 cesarean patients were randomly divided into A and B group. A group adopted patient controlled epidura analgesia(PCEA) micro-injection pump;B group using patient controlled intravenous analgesia(PCIA) mi-cro-injection pump,patients were observed and recorded for their pain, vomit disgustingly, exsufflation and the time of first urination or accidentally tube pull-out. Results The time of average surgery for A, B group were (53.5±8.5) min, (54.7±8.2) min, between the two groups was not significant (t=1.524, P>0.05) ; A group of visual Analog score (1.49±0.43) points were significantly higher than B group (1.13±0.56) points(t=2.119,P<0.05) ;the incidence of headache, dizziness, nausea and vomiting, skin itching(30.0%, 16.0% ,6.0%) in A group were signifi-candy higher than B group(16.0%,8.0% ,2.0%) (t=2.223, t=2.313, t=2.283, all P>0.05) ; the time of A first exhaust,voiding time[(24.56±8.77) h, (6.51±1.57) h] in A group were significantly higher than B group [(19.29±8.16)h,(4.06±1.76)h](t=2.445,t=2.415,P<0.05). Conclusion PCIA method was safe,effec-tive and allowed patients to have early activities after treatment and to promote early rehabilitation. It should be promo-ted for cesarean section.

Objective To determine mitochondria-associated signaling pathway on cell apoptosis in Leptospira interrogans infected murine mononuclear-macrophages. Methods A cell apoptotic model of L. interrogan serogroup Icterohaemorrhagiae strain Lai inducing apoptosis of murine mononuclear-macrophage line (J774A. 1) was established. Pathological changes of mitochondria in the infected cells were observed under transmission electron microscope. Mitochondrial potential and reactive oxygen species(ROS) levels in the infected cells were detected using JC-1 staining method and DCFH-DA fluorescent probe, respectively,and caspase-8/-9 activities in the infected cells were measured using commercial kits. and the apoptosis block effects of caspase inhibitors were determined by flow cytometry. Apoptosis in the infected cells and ap-optosis-blocking effect by caspase inhibitors were detected by flow cytometry. Western blot assay was adopted to examine the levels of cytochrome C (CytC) , AIF, EndoG and Smae in the mitochondria and cytoplasm.The transposition of either AIF or EndoG from the cytoplasm into cell nucleus was determined by immunoflu-orescence staining test. Results L. interrogans strain Lai could induce J774A. 1 cell apoptoais. In the in-fected cells, visible mitochondrial injury, declined mitochondrial membrane potential and elevated ROS level were presented. The activity of caspase-8 but not of caspase-9 was significantly increased. The caspase in-hihitors could not completely block the cell apoptosis. Both the AIF and EndoG was released from the mito-chondria and subsequently transferred from the cytoplasm into the nucleus in the infected cells. However,elevation of CytC level and Smac release in cytoplasm of the infected cells could not be found. Conclusion L.interrogans can induce apoptosis in the infected mononuclear-macrophage via AIF and EndoG belonging to caspase-independent pathway.

Objective To evaluate the efficacy of ultrasound?guided transversus abdominis plane ( TAP) block with different concentrations of ropivacaine for analgesia after cesarean section. Methods A total of 120 parturients, aged 24-31 yr, of American Society of Anesthesiologists physical statusⅠ or Ⅱ, weighing 64-73 kg, at 35 to 41 week gestation, scheduled for elective cesarean section, were randomly di?vided into 3 groups ( n= 40 each) using a random number table: 0?25% ropivacaine group ( group Ⅰ) , 0?20% ropivacaine group ( groupⅡ) , and 0?15% ropivacaine group ( groupⅢ) . A patient?controlled an?algesia pump was connected at the end of surgery, and the corresponding concentration of ropivacaine 1?5 mg∕kg was injected into the bilateral TAP under the guidance of ultrasound in each group. Visual analogue scale score was maintained ≤3. The number of attempt and the number of patients requiring rescue analge?sic were recorded at 48 h after surgery. The occurrence of TAP block?related complications was observed and recorded. Results Compared with groupⅢ, the number of attempt and the number of patients requi?ring rescue analgesic were significantly decreased in Ⅰ and Ⅱ groups ( P<0?05 ) . Compared with groupⅡ, the number of attempt and the number of patients requiring rescue analgesic were significantly de?creased in group Ⅰ (P<0?05). No TAP block?related complications were detected in the three groups. Conclusion Ultrasound?guided TAP block with 0?25% ropivacaine is helpful in improving the analgesic efficacy after cesarean section without serious complications.

Objective To observe the change of gastro-intestinal hormone levels about syndrome type of stagnation of liver qi, deficiency of spleen and stomach, liver qi stagnation and spleen qi deficiency in patients with irritable bowel syndrome (IBS), and to probe into the correlation between TCM syndrome type and gastro-intestinal hormone levels. Methods One hundred and three cases of patients with IBS were divided into liver qi stagnation group (35 cases), spleen and stomach deficiency group (30 cases) and liver qi stagnation and spleen qi deficiency group (38 cases), with normal group (10 cases) as control. Plasma motilin (MOT), somatostatin (SS) and cholecystokinin (CCK) were determined by radioimmunoassay. Result The plasma MOT level of spleen and stomach deficiency group was significantly higher than that in the normal controls (P

Objective:To find the rate of anxiety and depression in peritoneal dialysis (PD) patients,and the relationship between these emotion disorder and PD processes.Method:88 PD patients were studied, their mood state was evaluated with Hamilton Anxiety Scale and Hamilton Depression Scale. Nutritional indexes, residual renal function and dialysis adequacy, and inflammation status were also recorded as well as history of cardiac-cerebral vascular disease. These patients were followed for 2 years.Result:44.3% patients were detected as anxiety, and 56.8% depression. Higher SGA was associated with higher Hamilton anxiety/depression scale. Patients with higher anxiety and higher depression had lower serum albumin, lower protein intake, lower body index, and lower mid-arm muscle area and fat area. Anxiety and depression is related to cardiac-cerebral vascular diseases and diabetes.There was no significant relationship between Hamilton anxiety/depression score and patient's survival.Conclusion:There is a high rates of anxiety and depression in PD patients and it may be a potential risk factor of malnutrition.