OBJECTIVE To reinforce the personnel′s protection in supply division, to reduce the harmfulness of profession. METHODS The reasons of education, standard operation, dedicated supplies for protection, and building up wholesome institution were carried out. RESULTS The personnel′s consciousness of protection was reinforced and the arrangement of protection was carried out. Incidence wasn′t happened. CONCLUSIONS Better precaution and professional competency of personnel are the bases of protection for high quality work in supply division.

Bone Marrow Cells ; cytology ; physiology ; Humans ; Immunity ; Mesenchymal Stromal Cells ; cytology ; physiology

Child ; Complement C3 ; metabolism ; Cytokinins ; analysis ; metabolism ; Female ; Humans ; Immunoglobulin A ; immunology ; Immunoglobulin G ; immunology ; Interferon-gamma ; immunology ; Interleukin-12 ; analysis ; immunology ; Interleukin-2 ; analysis ; immunology ; Male ; Peptide Fragments ; immunology ; Respiratory Tract Infections ; epidemiology ; immunology ; virology ; Secondary Prevention ; Tuberculin ; analysis

Objective To investigate the protective effect of indole-3-carbinol (I3C) on radiation-induced mouse bone marrow hematopoietic cell injury and the involved mechanisms. Methods (1) The bone marrow nuclear cells (BMNCs) from CD45.1 subtype of C57BL/6J mice were collected by a density gradient centrifugation method. The BMNCs were pretreated with a series doses of I3C (0 mol/L, 10-8 mol/L-10-3 mol/L) and then exposed with radiation of 137Csγ-ray (doses of irradiation were 0 Gy, 1 Gy and 4 Gy). After 18-hour culturing, the bioluminescence method was used to detect the cell viability. (2) These cells were divided into control group and 10-6 mol/L I3C group. Both groups were received the irradiation (0 Gy, 1 Gy and 4 Gy) and inoculated into the methylcellulose semi-solid culture medium to incubate 7 days, the colony forming unit-granulocyte monocytes (CFU-GM) were observed. (3) Twenty-four CD45.2 subtype mice used as the receptor were exposed with 8 Gy radiation. The CD45.1 BMNCs were divided into control group, 4 Gy irradiation group, 4 Gy irradiation and 10-6 mol/L I3C group. Donor cells were harvested from C57BL/6J (CD45.1) mice after they received various treatments, and were then mixed with competitive BMNCs from C57BL/6J (CD45.2) mice. The mixed cells were transplanted into recipient mice (8 mice/group). Flow cytometry was used to analyze the proportion of donor cells in peripheral blood of receptor. (4) The cells were divided into control group, 10-6 mol/L I3C group, 1 Gy irradiation group, 1 Gy irradiation with 10-6 mol/L I3C group. After 24-hour culturing, Western blot assay was used to detect the expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1). Results (1) I3C showed a significant cytotoxic effect on the BMNCs when its concentration was above 10-4 mol/L. 10-7-10-6 mol/L I3C could reduce the radiation injury of BMNCs under the same dose of irradiation. Therefore, 10-6 mol/L I3C was chosen for subsequent experiments. (2) The CFU-GM was significantly higher in 10-6 mol/L I3C group than that of control group (P<0.05). (3) Results of flow cytometry showed that the proportion of donor cells in receptor was significantly higher in 4 Gy irradiation group than that of control group, which decreased the engraftment capability of irradiated HSCs (P<0.05), although the engraftment capability of irradiated HSCs improved after 10-6 mol/L I3C treatment. (4) I3C significantly enhanced the increased protein expression of Nrf2 and HO-1 caused by radiation (P<0.05). Conclusion I3C has a protective effect on hematopoietic cells following radiation-induced injury, which may be related with activating the Nrf2/HO-1 signal pathway.

OBJECTIVE:To establish a method for determining the content of paeoniflorin in Xiaohe syrup. METHODS:HPLC method was adopted. The Waters SunFire C18 column was used with the mobile phase of acetonitrile-0.1% phosphoric acid(12∶88, V/V)at the flow rate of 1.2 ml/min;the detection wavelength was 230 nm with column temperature at 30℃. Sample size was 10μl . R E-SULTS:There was a good linear relationship between the amount of paeoniflorin and peak area in the range of 0.061 0-1 . 2 2 0 8 μg (r=0.999 9). The RSDs of precision,stability and reproducibility tests were all less than 2% and the average recovery was 98.77%(RSD=1.09%,n=6). CONCLUSIONS:The method is simple,rapid and accurate and can be used for the content deter-mination of paeoniflorin in Xiaohe Syrup.

Objective To observe the effect and study intervenient action of Kuijieling Granules (KG) in treating ulcerative colitis (UC) during the active period by the clinical experiment. Methods The patients of active UC (damp-heat syndrome) were randomly divided into 2 groups (KG+SASP group and SASP group) to observe pathological changes of the mucosa,syndrome effect and main symptoms scores,and the protein expression of TLR4,CD14 and NF-?B p65 were detected by immunohistochemistry. Results KG+SASP group had better effect than SASP group in syndrome and mucosa (P0.05). There were outstanding differences among the pathologic grade of UC about IA of TLR4,CD14 and NF-?B p65. The expression of TLR4,CD14 and NF-?B p65 were decreased by treatment (P

Objective To observe the change of gastro-intestinal hormone levels about syndrome type of stagnation of liver qi, deficiency of spleen and stomach, liver qi stagnation and spleen qi deficiency in patients with irritable bowel syndrome (IBS), and to probe into the correlation between TCM syndrome type and gastro-intestinal hormone levels. Methods One hundred and three cases of patients with IBS were divided into liver qi stagnation group (35 cases), spleen and stomach deficiency group (30 cases) and liver qi stagnation and spleen qi deficiency group (38 cases), with normal group (10 cases) as control. Plasma motilin (MOT), somatostatin (SS) and cholecystokinin (CCK) were determined by radioimmunoassay. Result The plasma MOT level of spleen and stomach deficiency group was significantly higher than that in the normal controls (P

Objective To investigate the protective effect of 3,3′-diindolylmethane (DIM) on radiation-induced injury in mouse hematopoietic system. Methods Thirty C57BL/6 mice were randomly divided into control group, 2 Gy irradiation group and 2 Gy irradiation+DIM group (n=10 for each group). Mice of control group received sham irradiation, and the other two groups accepted 2 Gy 137Cs γ-ray total body irradiation. Mice in 2 Gy irradiation +DIM group were intraperitoneally injected 75 mg/kg DIM 30 min before irradiation. Mice of other two groups were treated with reference solution. After 7 d and 15 d of 2 Gy irradiation, the peripheral blood samples were collected to count the number of bone marrow nuclear cells (BMNCs). The level of reactive oxygen species(ROS) was measured by DCFH-DA. The levels of colony forming units-granulocyte-macrophage (CFU-GM) were also detected. Results The numbers of white blood cell (WBC), platelet count (PLT), BMNCs and CFU-GM were significantly decreased and the ROS level of bone marrow cells increased significantly in the irradiated group than those of control group (P<0.05). Compared to 2 Gy irradiation group, the numbers of WBC, PLT, BMNCs and CFU-GM were significantly increased in 2 Gy irradiation +DIM group, and the level of ROS was decreased significantly (P<0.05). Conclusion DIM has a protective effect on hematopoietic cells following radiation-induced injury, which may be related with the decreased ROS level.

Objective To evaluate the clinical value of pre-operative double embolization for nasopharyngeal angiofibroma. Methods Digital subtraction angiography(DSA) was carried out in 7 cases of nasopharyngeal angiofibroma. In six cases,the supplying arteries originated from the maxillary artery and the branches arising from internal carotid artery underwent double embolization-direct percutaneous puncture embolization and selective embolization of blood supplying arteries. In another case,the supplying artery originated from maxillary artery was only undertaken selective embolization for the feeding arteries. Results All the tumor masses reduced in vonlume obviously or even disappeared on the angiography after embolization. Conclusions Pre-operative double embolization for nasopharyngeal angiofibroma could be a necessary additional treatment method. (J Intervent Radiol,2006,15: 342-344)

Objective To evaluate the effects of different doses of epidural capsaicin on pain threshold, neurological function and spinal neurons.Methods Thirty-six male New Zealand white rabbits weighing 2.5-3.0 kg were randomly divided into 4 groups (n = 9 each): control group and 3 capsaicin groups (Cap 1, 2, 3). The animals were anesthetized with 10% chloral hydrate 50-75 mg?kg-1 i.v. . An intrathecal catheter was inserted at L6,7 interspace and correct placement was confirmed by outflow of CSF. 1 ml of 0.1 % ( Cap1) , 0.25 % ( Cap2 ) or 0.5% (Cap3 ) capsaicin was injected intrathecally 24 h after IT catheter was placed. Threshold to noxious thermal stimuli was measured and gait of the hind limbs were assessed using Johnson score (5 = normal, 0 = completely paralyzed), before and 1, 3, 7, 10, 15, 20, 25, 30 days after IT injection. On the 2nd day after IT injection 3 animals were killed in each group and the lumbar segment of spinal cord (L6,7) was immediately removed for light and electron microscopic examination. Results The threshold to noxious thermal stimuli was significantly higher in the 3 capsaicin groups than in control group ( P