No abstract available.
Infant, Newborn ; Metabolism, Inborn Errors* ; Neonatal Screening*

No abstract available.
Firearms* ; Forensic Ballistics* ; Wounds and Injuries*

No abstract available.
Fistula* ; Hemorrhoids* ; Humans

No abstract available.
Humans ; Male* ; Stem Cells*

No abstract available.
Wounds and Injuries*

No abstract available.
Tomography, Emission-Computed, Single-Photon*

No abstract available.
DNA* ; Genes, Synthetic* ; Genomics* ; Oligonucleotide Array Sequence Analysis*

No abstract available.
Fistula*

Allogeneic demineralized bone powder has been shown to support the osteogenesis in a variety of bone defect and extra-osseous sites. Previous several experiments have been carried out in animal models which have time-consuming and various problems. Therefore author designed some cytotoxicity tests by using of 5-diphenyl dimethyltetrazolium bromide(MTT) assay, neutral red(NR) assay, lactate dehydrogenase(LDH) activity test, sulforbodamine B (SRB) assay, and alkaline phosphatase(ALP) assay. And these method seemed to be a rapid, repeatable and believable reaction. To establish the osteogenesis capacity of allogeneic bone according to various bone preparation(defatting and demineralization), author studied the effect of allogeneic bone on well-established rat 3T3 fibroblasts and MC3TEl osteoblasts in culture to evaluate a cytotoxicity and examined the surface of allogeneic bone with scanning electron microscopy(SEM) for detecting the porositv. Defatting Demineralized Bone Powder(DDBP0 and Non-Defatting Demineralized Bone Powder(NDDBP) of particle size 212mum were prepared from rat calvarial bone and long bones. 1. Cell counts of rat 3T3 fibroblasts and MC3T3E1 osteoblasts were greater in DDBP enriched medium when compared with medium with NDDBP. Cell counts with DDBP were to different from controls. 2. Author found a little differences in cytotoxicity on 3T3 fibroblast and MC3T3E1 osteoblasts between DDBP and NDDBP with MTT assay, NR assay, LDH activity test, SRB assay, Generally, DDBP was represented less cytotoxicity than NDDBP. 3. ALP assay with MC3T3E1 osteoblasts in culture presented the less cytotoxicity and more crowding around the DDBP than NDDBP. With these findings, defatting procedure on making the demineralized bone powder have a merit for supporting osteogenesis. I should be suggested that cell culture with DDBP and NDDBP is a good method for studying induced osteogenesis.
Animals ; Cell Count ; Cell Culture Techniques ; Crowding ; Fibroblasts ; Lactic Acid ; Models, Animal ; Osteoblasts ; Osteogenesis* ; Particle Size ; Rats ; Transplants*

To try to determine the type of medical service wanted by the rural population, in Chungnam Province, a survey amongst the populations of 6 counties was conducted; Within the 6 counties, 2 local communities, which had no access to local medical services, were surveyed. The 12 communities were actively involved in Sae-Maul activities, and total number of households surveyed, was 822. The survey was conducted over a 1 month period, from July 16th, 1976, thru August 20th, and the followings are the results, summarized. 1. The largest number of respondents desired a combination of Public Health Center and Country Hospital, followed in order by Private Clinic and Modern Medical Facility. 2. The respondents, aged under 40 years, desired the Private Clinic type medical service, whilst those over 40 years of age, wanted the County Hospital, and as the numbers in this age bracket, were larger, so the ratio was much higher. 3. Sex, educational background, and occupation did not play any particular emphasis in the decisions. 4. Monthly income affected the responses to the survey. These in the lower-income bracket, wanted the County Hospital, and the ratio was high. These in the high income bracket desired the Modern Medical Service, accordingly. Those with an income of 50,000 won or less, amongst the low-income bracket, favored the Public Health sub-center type of service. The ratio for this service was very high.
Chungcheongnam-do ; Surveys and Questionnaires ; Family Characteristics ; Hospitals, County ; Occupations ; Public Health ; Rural Population