Objective:To investigate the diagnosis and treatment principles of multiple primary colorectal carcinoma(MPCC).Methods:A retrospective analysis was made on 63 patients with MPCC admitted from January 1993 to January 2007 in our hospital. Results:The incidence of MPCC was 2.1%(63/3 021) with 31 cases being synchronous carcinoma(SC) and 32 cases diagnosed as metachronous carcinoma(MC). Male to female ratio was 2.5:1.Most tumors were located in rectum and sigmoid colon.A total of 34 cases(54.0%) were diagnosed by fiberoptic colonoscopy before operation.The diagnosis rate of SC by fiberoptic colonoscopy before operation and during operative exploration were all 38.7%(12 cases). The diagnosis rate of MC by fiberoptic colonoscopy before operation and during operative exploration were 68.7%(22 cases) and 6.25%(2 cases) respectively.The overall 5-year survival rate of the patients in SC was 45%(9/20),the overall 5-year survival rate of the patients in MC after the first operation was 72.4%(21/29). Conclusion:Clinician should improve the recognition of MPCC.Patients with colorectal carcinoma should undergo routine fibercolonscopy and standard radical excision. Periodic follow-up for the high-risk group should be highlighted.

Endothelium, Vascular ; cytology ; Humans ; Mucocutaneous Lymph Node Syndrome ; complications ; pathology ; Neovascularization, Pathologic ; Stem Cells ; cytology

ObjectiveTo investigate the effect of hyperbric oxygen (HBO) on infarct volume and relevant mechanism after permanent focal cerebral ischemia in adult rats.MethodsRat model of focal cerebral ischemia induced by intraluminal filament occlusion of middle cerebral artery (MCA) was used. HBO(2.0 ATA) was applied to HBO group. Infarct volume, matrix metalloproteinase 2 (MMP-2) and MMP-9 were detected at 6h, 24h, 48h, 72h,120h and 10d after ischemia.ResultsThe infarct volume obviously decreased at 120h and 10d and expression of MMP-9 lowered at 48—120h in HBO groups. There was no significant change in MMP-2.Conclusion HBO can reduce infarct volume after cerebral ischemia, which may be related to downregulation of MMP-9 levels.

ObjectiveTo observe the clinical therapeutic effect of intra-articular injection with sodium hyaluronate (SH) on osteoarthritis of the ankle.Methods25 patients with osteoarthritis of the ankles (36 ankles) were treated with SH intra-articular injection 2 ml every week for 5 weeks. The degree of pain, swelling and functional disturbance of the ankles were observed to evaluate the effect.ResultsAfter treatment, the degree of pain, swelling and functional disturbance of the ankles improved (P<0.05～0.01). The total efficiency rate was 91.61%.ConclusionSodium hyaluronate injected intra-articular is an effective, ideal and safe treatment for osteoarthritis of the ankle.

BACKGROUND: The pain caused by osteoporosis and osteoporotic fracture is usually treated with non-steroidal anti-inflammatory drugs (NSAIDs) in clinic, which has been reported that dysfunction of liver and kidney will follow the use, but what has happened in the tissue of liver and kidney is not reported. OBJECTIVE: To study the effect of NSAIDs on the tissue of liver and kidney in osteoporotic fracture rats. DESIGN, TIME AND SETTING: Randomized controlled animal experiment. The experiment was completed in the Animal Experiment Center in Shanghai University of Traditional Chinese Medicine from August 2007 to February 2008.MATERIALS: A total of 24 female SD rats, 8-month-old, weighing 300-320 g, were randomly divided into 3 groups, saline group,diclofenac sodium (fracture before medicine) group and diclofenac sodium (fracture after medicine) group, with 8 rats in each group.METHODS: The rats were housed for 3 months after ovariectomized to establish osteoporosis models. Rats in the saline group and diclofenac sodium (fracture before medicine) group were administrated saline and diclofenac sodium after transverse osteotomy at the middle shaft of bilateral femur respectively; in the diclofenac sodium (fracture after medicine) group were femur.MAIN OUTCOME MEASURES: The histological observation of liver and kidney was performed at weeks 2, 3, 4 and 6 after fracture.RESULTS: In the diclofenac sodium (fracture before medicine) group, diclofenec sodium caused the inflammatory response at glomeruli, which exhibited expansion of tubular lumen, edema of epithelial cells, disappeared cell nuclei, degeneration and necrosis of renal tubule, cell debris and drugs crystals accumulated in the tubular lumen, congestion and inflammatory cell infiltration of renal interstitium. Administrating diclofenac sodium may cause the inflammatory response at portal area, indistinct structure of hepatic Iobule, hepatic cells edema, steatosis and necrosis. Administrating diclofenac sodium before osteoporotic fracture also resulted in tissue lesions in kidney and liver, the damage would continue about 3 weeks.CONCLUSION: The diclofenac sodium cause histological lesions of kidney and liver in osteoporotic rats, especially in kidney. The histological lesions of kidney and liver are inreversible after administrating diclofenac sodium for a long time.

BACKGROUND:The pain caused by osteoporosis and osteoporotic fracture is usually treated with non-steroidal anti-inflammatory drugs (NSAIDs) in clinic,which has been reported that dysfunction of liver and kidney will follow the use,but what has happened in the tissue of liver and kidney is not reported.OBJECTIVE:To study the effect of NSAIDs on the tissue of liver and kidney in osteoporotic fracture rats.DESIGN,TIME AND SETTING:Randomized controlled animal experiment.The experiment was completed in the Animal Experiment Center in Shanghai University of Traditional Chinese Medicine from August 2007 to February 2008.MATERIALS:A total of 24 female SD rats,8-month-old,weighing 300-320 g,were randomly divided into 3 groups,saline group,diclofenac sodium (fracture before medicine) group and diclofenac sodium (fracture after medicine) group,with 8 rats in each group.METHODS:The rats were housed for 3 months after ovariectomized to establish osteoporosis models.Rats in the saline group and diclofenac sodium (fracture before medicine) group were administrated saline and diclofenac sodium after transverse osteotomy at the middle shaft of bilateral femur respectively;in the diclofenac sodium (fracture after medicine) group were administrated 5 mg/(kg?d) diclofenac sodium for 3 weeks,then received transverse osteotomy at the middle shaft of bilateral femur.MAIN OUTCOME MEASURES:The histological observation of liver and kidney was performed at weeks 2,3,4 and 6 after fracture.RESULTS:In the diclofenac sodium (fracture before medicine) group,diclofenac sodium caused the inflammatory response at glomeruli,which exhibited expansion of tubular lumen,edema of epithelial cells,disappeared cell nuclei,degeneration and necrosis of renal tubule,cell debris and drugs crystals accumulated in the tubular lumen,congestion and inflammatory cell infiltration of renal interstitium.Administrating diclofenac sodium may cause the inflammatory response at portal area,indistinct structure of hepatic lobule,hepatic cells edema,steatosis and necrosis.Administrating diclofenac sodium before osteoporotic fracture also resulted in tissue lesions in kidney and liver,the damage would continue about 3 weeks.CONCLUSION:The diclofenac sodium cause histological lesions of kidney and liver in osteoporotic rats,especially in kidney.The histological lesions of kidney and liver are inreversible after administrating diclofenac sodium for a long time.

Absorption ; Ammonia ; chemistry ; Environmental Monitoring ; methods ; Materials Testing ; Silica Gel ; chemistry

Thyroid peroxidase (TPO) , originally described as thyroid microsomal antigen, is present on the apical surface of thyroid follicular cells and is an antigen involved in cell mediated cytotoxicity. TPO evokes high-affinity, IgG-class autoantibodies (TPOAbs) and TPO-specific T cells that are markers of thyroid infiltration or implicated in thyroid destruction, respectively. Enzyme-linked immunosorbent assay is used most frequently for TPOAb detection and quantification. The other conditions associated with TPOAbs include pernicious anemia, connective tissue disorders, diabetes, inflammatory bowel disease, mood disorders, and fertility-related problems such as miscarriage, infertility, in vitro fertilization failure, pre-term delivery, and postpartum thyroiditis. The detection of TPOAhs is recommended in the investigation of goitre, diagnosis of Graves' disease and Hashimoto's thyroiditis, and the prediction of risk of developing hypothyroidism during subclinical thyroid disease.

Polymerase chain reaction (PCR) is one of the common techniques in molecular biology, which can amplify nucleic acids through the cycle of denaturation, annealing and extension. Based on the principle of common PCR, rapid PCR is to realize the amplification of nucleic acids in less time without affecting the specificity, sensitivity and fidelity of the reaction. A lot of research work in this field has been going on in recent years. This article will make a review of the development of rapid PCR with emphases on the improvement of DNA polymerase, the choice of additives and the improvement of thermocyclers.