Objective: The imaging diagnosis of early liver abscess is difficult. This study is to evaluate the diagnostic value of DWI, T2WI and dynamic enhanced MR imaging for early liver abscess. Methods: DWI, T2WI and dynamic enhanced imaging scans were performed in 17 patients with 17 early liver abscesses. The signal intensities and ADC values of necrotic area, non-necrotic area and normal liver were measured and compared. Results: The ADC value of the necrotic areas of liver abscesses ([0.88±0.09] mm2/s) was significantly lower than those of normal liver ([1.31±0.12] mm2/s,P<0.05) and non-necrotic area of liver abscesses ([1.43±0.18] mm2/ s,P<0.05); there was also significant difference between the ADC values of the normal liver and non-neocrotic area (P<0.05). The necrotic areas had a hyperintensity characteristic on DWI, T2WI and a hypointensity characteristic on T1 WI; no enhancement was found on dynamic enhanced imaging. The non-necrotic areas had moderate hyperintensity on DWI, T2 WI and hypointensity on T1 WI; there was obvious enhancement on dynamic enhanced imaging. Conclusion: The early liver abscess is characterized on magnetic resonance imaging. The DWI scan is helpful for diagnosis of early liver abscess.

Objective: To investigate the value of magnetic resonance cholangiopancreatography(MRCP) in the diagnosis of pancreas divisum by comparing with endoscopic retrograde cholangiopancreatography (ERCP). Methods: The MRCP and ERCP images of 8 patients with pancreas divisum were retrospectively analyzed. The diagnostic accuracy and findings by MRCP were compared with those by ERCP. Results: MRCP had a diagnostic accuracy of 87.5%(7/8) based on the result of ERCP. ERCP displayed the dominant dorsal pancreatic ducts in all 8 cases and ventral pancreatic ducts in 6 cases; MRCP also displayed the dominant dorsal pancreatic ducts in all 8 cases, but the ventral pancreatic ducts only in 3 cases. Conclusion: As a non-invasive technique, MRCP has important clinical value in the diagnosis of pancreas divisum.

Ptosis is one of the most common diseases in oculoplastics. Surgical treatment is the only way to correcting ptosis. Most of doctors paid special attention to surgical technique, rather than to some related issues of operation. Timing is crucial for ptosis surgery. Both cosmetic and function need to be considered for a success surgery, the evaluation of extraocular muscle disorder is impor- tant for surgical result and postoperative complications.

Huaier (Trametes robiniophila) has been widely used as an adjuvant drug for cancer treatment in China. The anti-cancer effect of Huaier extract has been confirmed in liver cancer, lung cancer, breast cancer, ovarian cancer, gastric cancer, and so on. The main mechanisms by which Huaier exerts an anti-neoplastic effect include inhibition of the growth and proliferation of cancer cells, induction of apoptosis of cancer cells, suppression of angiogenesis, inhibition of the invasion and migration of cancer cells, regulation of oncogenes and tumor suppressor genes expression, improving immunity, and reversal of drug resistance in cancer cells. In order to provide references for further study and clinical application on anti-tumor effect of Huaier, the latest research progress on anti-tumor effect of Huaier in recent years is summarized in this paper.
Animals ; Antineoplastic Agents ; pharmacology ; Humans ; Trametes

Adolescent ; Adult ; Athletic Injuries ; Clinical Clerkship ; Female ; Humans ; Nurses ; Occupational Injuries ; Young Adult

A simple assay for detection of phospholipase C (PLC) activity was developed based on a fluorescence liposome probe using the Liss Rhod PE-loaded phospholipid liposomes.The liposome probe was prepared by the coassembly of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and fluorescent lipid (Liss Rhod PE).The probe showed very low background fluorescence due to fluorescence self-quenching effect of Liss Rhod PE.As the PLC enzyme selectively digested lipid, the Rhod fluorescence was recovered from its quenched state, leading to the sensitive detection of PLC.This assay provided a limit of detection (at a signal-to-noise ratio of 3) of 2 U/L for PLC.In the presence of PLC inhibitor, the fluorescent response of the sensor for PLC decreased, indicating that the assay could also be used for screening PLC inhibitors.

Objective To investigate the relationship among methotrexate(MTX) plasma concentration,dosage,clinical effecicy and toxicity, and to evaluate it′s clinical significance.Methods MTX was measured by a flurorescence polarization immunoassay in plasma samples obtained from acute lymphoblastic leukemia(ALL) patients treated in different doses of MTX, and these results were analyzed combined with clinical manifestations.Results 1.The average of plasma concentration at 24 hours increased with the increasing doses of MTX. The relapse rate decreased with increased plasma concentration;2.The cerebrospinal fluid(CSF) concentrations prior to the intrathecal MTXinstillation were all below the effective concentration, so the intrathecal MTX instillation was needed;3.No severe toxicity was observed in the study, because the plasma concentration was below the high risk.Conclusion The study of MTX plasma concentration provides us an objective basis for the individualized chemotherapy.

Objective To compare efficacy and side effects of intravenous versus oral indomethacin treatment for symptomatic patent ductus arteriosus (PDA) in preterm infants.Methods Fourty-nine preterm infants were reviewed retrospectively who were diagnosed as having symptomatic PDA confirmed by echocardiography.According to the using type and approach that were divided into 2 groups (intravenous group,n=21;oral group,n=28) and their doses and intervals were same.The rates of ductal closure and side effects were compared in 2 groups.Results There were no significantly different between 2 groups in single ductal closure and complicating other diseases. Soon closure of intravenous group was higher significantly than oral group [61.9 %(13/21) vs 28.6 %(8/28),P

Objective To study the relationship between clinical diagnosis and children ventricular premature beat(VPB) electrocardiogram location and morph.Methods Both organic heart disease and without organic heart disease relationship with 109 cases of children ventricular premature beat electrocardiogram location and morph were retrospectively analyzed.Results Children ventricular premature beat location shows that organic heart disease mostly results from left ventricle, without organic heart disease often comes from right ventricle. There was significant difference between above two groups (? 2=37.25 P

Objective Insulin-like growth factor-1 receptor (IGF-1R),similar to insulin receptor,is one of the families of re- ceptor tyrosine kinases.,which has been found to be overexpressed in a variety of cancer.It is the main proliferation and survival sig- nal molecule in cancer cell and plays an important role in cancer growth and progress.Blocking signal transduction of IGF-1R by vari- ous strategies can suppress tumor growth and induce regression of established tumor.This study is to construct the siRNA expression vector targeting IGF-1R and to evaluate its ability to induce cell apoptosis in human lung cancer cells.Methods Two siRNA expres- sion vector,pENTR/U6-shRNA-1 and pENTR/U6-shRNA-2 targeting IGF-1R,were constructed using pENTR/U6 vector,and a vector targeting hieiferase gene,pENTR/U6-shRNA-Iuc,was constructed as control.After vectors were transfected into A549 for 48h, knockdown of IGF-1R mRNA and protein and Akt phosphorylation were accessed,and DNA ladder and flow cytometry were used for cell apoptosis.Results siRNA expression vectors targeting IGF-1R were successfully constructed,which was confirmed by PCR and DNA sequencing,pENTR/U6-shRNA-1 and pENTR/U6-shRNA-2 demonstrated the expression were (22.1?2.5) % and (80.1? 3.9) % in IGF-1R mRNA level,(15.2?3.1)% and (47.1?4.1)% in protein level,respectively,compared with pENTR/U6- shRNA-luc.Suppression of IGF-1R by pENTR/U6-shRNA-1 blunted Akt phosphorylation,increased cell apoptosis induced by 3% ethanol,and retained 77.5 % A549 cells in the G0/G1 phase.Conclusion siRNA expression vector targeting IGF-1R can effectively suppress the expression of IGF-1R expression in A549.This study suggests that DNA vector-based RNAi has the potential to be effec- tive and practical cancer gene therapy strategy.