Many carcinomas have an active mononuclear cell infiltrates surrounding tumor. Various in vitro assays have shown that cellular constituents of peripheral blood mononuclear cells(PBMC) can alter growth of carcinoma cell line. Author compared the effects of normal fibroblasts on squamous cell carcinoma cell line(SCL-1) along with those of sctivated and/or nonactivated PBMC on SCI 1 using a skin equivalent system. This system prevents direct cellular contact by growing SCL-1 on an overlying Millicell-HA membrane and normal fibroblast or supernatants of PBMC in a lower chamber. Normal fibroblasts enhanced the outgrowth of SCL-1 and induced a more organized phenotype of SCL-1. Supernatants from nonstimulated PBMC suppressed outgrowth of SCL 1, and concanavalin A stimulated PBMC supernatants alterd rnorphology of cultured SCL-1 from a disorganized phenotype to a more organized phenotype. It is concluded that fibroblasts and PBMC may affect the growth and differentiation of SCL-1 via their mediators(cytokines)
Carcinoma, Squamous Cell* ; Cell Line* ; Concanavalin A ; Fibroblasts* ; Membranes ; Phenotype ; Skin

One hundred patients of osmidrosis were treated by surgical procedure at the Department of Dermatology, Wonkwang University Hospital. The methods of operative treatment were elliptical excision for 37(74), bipedicle incision with apocrine glancl undercutting for 14(28), and single incision wit.h apocrine gland undercutt,ing for 49 (98). The results were summarized as follows : 1. Of the 74 axillae treated by elliptical excision, one occurred recurrence, and 1.7 showed complication, hematoma in 6, infection and delayed union in 4, each necrosis and severe scar in one each, 2. Of the 28 axillae treated by bipedicle incision with apocrine gland undercutting, 6 showed complication; hematoma in 4, infection and delayed union in one each 3. Of t..he 98 axillae treated by single incision with apocrine gland undercutting, 5 showed complication, hematoma in 3, infection and delayed union in one each In conclusion, single incision with apocrine gland undercutting was found to be more excellent compared with the other surgical methods.
Apocrine Glands ; Axilla ; Cicatrix ; Dermatology ; Hematoma ; Humans ; Necrosis ; Recurrence

Forytfour-year-old female inpatient, who was gravely ill, developed vesicobullae on the greenish patches surrounded by narrow red to brown halo in the left lower extremity. Two years ago, she had treated by surgical operation and radiation therapy for cervix cancer. Blood and bullous fluid cultures showed Pseudomonas aeruginosa. She developed gangrenous and mummificated skin lesions after septic shock therapy but died due to disseminated intravascular coagulation.
Disseminated Intravascular Coagulation ; Female ; Gangrene* ; Humans ; Inpatients ; Lower Extremity ; Pseudomonas aeruginosa ; Pseudomonas* ; Shock, Septic ; Skin ; Uterine Cervical Neoplasms

No abstract available.

No abstract available.
Autonomic Pathways* ; Rectal Neoplasms* ; Urodynamics*

The authors dissected and examined a horseshoe kidney from the cadaver of a 54-year-old Korean female. The results were as follows. Other congenital anomalies or complications were not found, and no renal stones showed on plain radiography of the horseshoe kidney. The horseshoe kidney was located in the area between the 12 th thoracic vertebra and the 4th lumbar vertebra, with the superior extremity of the left kidney 10mm more inferior than that of the right. The isthmus connecting the bilateral kidneys was located at the level of the 3rd lumbar vertebra. The size of the kidney was 102mm × 52mm × 44mm (right) and 108mm × 62mm × 34mm (left), and the superoinferior and anteroposterior lengths of isthmus were 22mm and 10mm, respectively. The abdominal aorta and inferior vena cava passed posteriorly to the isthmus, with the inferior mesenteric artery and lumbar splanchnic nerve passing anteriorly. Some grooves were found on the anterior surface of the bilateral kidney. The hilum of the right kidney faced the anteromedial direction and that of the left kidney faced the anterolateral direction. At the hilar plane, the right renal arteries and veins passed anteriorly and posteriorly to the renal pelvis ; the left renal arteries passed posteriorly to the renal pelvis, with the left renal veins passing anteriorly and posteriorly. Three branches of the right renal arteries passed renal hilum, while two branches did not, and two branches of the left renal arteries passed renal hilum, while six branches did not. The two arteries arising from the aortic bifurcation were distributed to the isthmus. The number of renal veins passing the renal hilum were three in the right, and two in the left. The right and left ovarian veins drained to the renal veins. There were 12 minor calyces distributed normally in the right kidney, 13 minor calyces distributed radially in the left kidney, and 3 minor calyces in the isthmus, composed of parenchyme. The left portion of the horseshoe kidney was concluded to have developed poorly, on the basis of incomplete ascension and abnormal rotation during development, the imperfect configuration of the renal shape, and the abnormal distribution of the renal vessels and renal calyces.
Aorta, Abdominal ; Arteries ; Cadaver ; Extremities ; Female ; Fused Kidney* ; Humans ; Kidney ; Kidney Pelvis ; Mesenteric Artery, Inferior ; Middle Aged ; Radiography ; Renal Artery ; Renal Veins ; Spine ; Splanchnic Nerves ; Veins ; Vena Cava, Inferior

As a new culture mediwn for the Ciliate, Balantidium coili, isolated from a plantar ulcer of a leprosy patient, Sabouraud glucose agar medium(SGA) had several advantages: 1. it was easily made, and 2) the culture grew faster and longer than any previriusly discovered media. Of the contents of SGA, dextrose and peptene are essential nutrient, for the growth of Balantidium coli. When we cultivated Balantidiuum coli. we founund that it produced a nuueoid eubstance and a sweet smilling arcma, forming a colony like tacteria The new straining metlnd for Balantiduin coli. were 3% iodine and carbol-fuchsin stain which atained trophazaite, and cysts more sirnply and distinctly, retaining their original form.
Agar ; Balantidium* ; Ciliophora* ; Foot Ulcer* ; Glucose ; Humans ; Iodine ; Leprosy*

BACKGROUND: Staphylococcus aureus and group A streptococci are the most common etiologic agents in cellulitis, but occasionally many other bacteria are also identified. The positive rate of bacterial cultures taken from the skin lesion are low. OBJECTIVE: This study aims to improve the positive culture rate in patients with cellulitis by using skin biopsy specimens in several kinds of media. METHODS: Skin biopsy specimens taken from 54 patients with cellulitis were cultured in 4 functionally-different types of media (blood agar, MacConkey agar, chocolate agar, thioglycollate broth). Positive culture rates were evaluated in each medium and cultured bacteria were identified. Clinical characteristics were also studied, including age, sex, affected site, and history of previous treatment. RESULTS: The sex ratio of males to females was 2.9: 1 and mean age was 49 years. The most commonly-involved site was the lower extremities (42.6%), followed by the upper extremities (13.0%), head and neck (9.3%), and trunk (1.9%). Patients who had received previous antimicrobial treatment numbered 31 cases (57.4%). Of the 23 patients who had received no previous antimicrobial treatment, 13 patients (56.5%) had positive cultures. The most common pathogens were S. aureus and Streptococcus sp. (59.1%), but seven different genus of bacteria were also isolated from 9 patients (40.9%). Thioglycollate broth yielded a high positive culture rate (38.9%) among the 4 types of culture media. CONCLUSION: It is suggested that the bacterial culture of skin biopsy tissue from four functionally-different types of media is a useful method for improving positive bacterial culture rate in patients with cellulitis.
Agar ; Bacteria ; Biopsy* ; Cacao ; Cellulitis* ; Culture Media ; Female ; Head ; Humans ; Lower Extremity ; Male ; Neck ; Sex Ratio ; Skin* ; Staphylococcus aureus ; Streptococcus ; Upper Extremity

We herein report three cases of pararneatal urethral cyst, which developed on the male urethral meatus. These cysts occurred since birth or childhood, and did not interfere with urinary of sexual function. Histologically, these cysts were lined by pseudostratified columnar epithelium with variable thickness. No recurrence was evident after simple excision.
Epithelium ; Humans ; Male ; Parturition ; Recurrence

Ewing's sarcoma is a highly malignant, small, round cell tumor that usually affects long bones. The acral part of the extremities is a very rare primary site for this neo plasm. A fifteen-year-old girl was seen for a lobulated, dome-shaped, 2.7cm diameter, denuded mass on the distal phalanx of the right middle finger which had increased in size over a 14-month period. The radiological featuies of the hand showed a cortical brick of distal phalanx and surrounding soft tissue mass. Histologically, the biopsy specimen showed sheets of small round to oval cells with scanty cytoplasm, that were closely packed and separated into lobules by trands of fibrous tissue. A periodic acid-Schiff stain demonsirated glycogen in the cytoplasm of the tumor cells. Electron microscopy showed large aggregates of glycogen in the cytoplasm of the neoaplastic cells. Immunohistochemical stains revealed positive staining for vimentin, glial fibrillary acid potein, and neuron specific enolase, stains for S-100, Factor VIII, and cytokeratin were negative.
Biopsy ; Coloring Agents ; Cytoplasm ; Extremities ; Factor VIII ; Female ; Fingers ; Glycogen ; Hand ; Humans ; Keratins ; Microscopy, Electron ; Phosphopyruvate Hydratase ; Sarcoma* ; Sarcoma, Ewing ; Vimentin