A collodion baby is born with a tough, inelastic parchment-like membrane covering the whole body surface. As the meinbrane fissures and peels, a more characteristic ichthyosiform change is evident beneath the collodion membrane. Uncommonly, normal skin is found under it. We herein present two cases af collodion baby, which were confirmed as a sporadic case of lamellar ichthyosis and a case of lamellar exfoliation of the newborn, respectively, after long-term clinical observation. It is necessary that detailed genetical and molecular biological studies should be perforrned in order to elucidate the fur:damental, molecular changes that cause these dramatic cutaneous changes.
Collodion* ; Humans ; Ichthyosis, Lamellar ; Infant, Newborn ; Membranes ; Skin

Normal values of CSF gases in young Koreans were reported by Song and Jeon as follows: PcsfO2 76.5+/-8.6 torr and PcsfCO2 44.9+/-3.6 torr. The pH of the CSF was 7.329 +/-0.017, HCO2 was 23.6+/-1.8mEq/L and the B.E. was -2.2+/-1.5. The CSF and arterial and venous blood gases of a group a relatively healthy patients aged 60~80 years scheduled for elective surgery were measured. Prior to the induction of anesthesia, lumbar tapping was performed at a level of L3-4 using a 22 gauge needle and CSF samples were obtained. Then, radial arteiral blood samples were taken. The subclavian vein was cannulated with a 20 gauge catheter and venous blood samples were collected. The gas values of this group were compared with those of the young age group as shown in the tables and figures. The results of this study were as follows: PcsfO2 was 72.76+/-10.49 otrr and PcsfO2 was 44.52+/-3.5 torr. The pH of CSF was 7.318+/-0.04. HCO3- was 22.62+/-2.5 and B.E. was 3.07+/-2.4.
Anesthesia ; Catheters ; Gases ; Humans ; Hydrogen-Ion Concentration ; Music ; Needles ; Reference Values ; Subclavian Vein

No abstract available.
Fever* ; Humans

A continuous leakage in association with hypovolemia and hypotention of the cerebrospinal fluid is the primary cause of a post-spinal headache. The spinal blood patch is known to be the best choice of treatment for a severe postspinal headache, and measurements of cerebrospinal fluid pressure with saline injection into the lumbar spinal space have been reported. However, a dynamic correlation of the pressure change between epidural and CSF pressure after the epidural injection of the volume has not been known. This study was primarily carried out to investigate dynamic changes and the correlation between epidural and CSF pressure with and without epidural volume injection so that it would be helpful to understand the mechanism as to the immediate and permanent cure, and recurrence of post-spinal headache. Twenty cases were divided into two groups: Group I (control): Normal CSF and epidural pressure were measurd in a sitting position (10 cases) and in a lateral position (10 cases). Group II: CSF and epidural pressure after the injection of 2% lidocaine 10 ml were measured in a sitting position (10 cases) and in a lateral position (10 cases). The differences between CSF and epidural pressure from groups I and II were calculated. The rusults were as follows. CSF pressure: The mean Mean opening pressure was 37.3+/-4.2 cm H2O, mean pressure after injeciton was 41.3+/-6.1 cm H2O and pressure rise was 4 cm H2O (10.7%) in the sitting position, and the mean opening pressure was 9.3+/-3.8 cm H2O mean pressure after injection was 13.9+/-5.2 cm H2O and pressure rise was 4.6 cm H2O (49.5%) in the lateral position. Epidural pressure: The mean initial pressure was 5.82.6 cm H2O, mean pressure after injection was 16.9+/-12.4 cm H2O and pressure rise was 22.7 cm H2O (391.3%) in the sitting posittion, and mean initial pressure was 6.1+4.0 cm H2O, mean pressure after injection was 9.5+ 7.9 cm H2O, and pressure rise was 15.6 cm H2O (255.7%) in the lateral position. Difference between CSF and epidural pressure (CSF pressure-epidural pressure, cm H2O): The mean pressure difference in the control group was 43.1+5.7 cm H2O and mean pressure difference after injection was 24,4+/-12.4 cm H2O in sitting position, and mean pressure difference in the control group was 15.4+/-4.1 cm H2O and mean pressure difference after injection was 4.4+/-10.1 cm H2O in lateral position. It is obvious that the injection of 10 ml of 2% lidocaine reduced the pressure difference greatly between the subarachnoid and epidural spaces. These results indicate that the volume of 10 ml is suitable for an epidural blood patch and it suggests that the patient must be placed in a supine or lateral position immediately after an epidural blood patch is done because of the equalizing of the pressure difference. However, there was still a small difference in pressure between the two compartments: The CSF pressure being higher than the epidural pressure.
Blood Patch, Epidural ; Cerebrospinal Fluid ; Cerebrospinal Fluid Pressure ; Epidural Space ; Headache ; Humans ; Hypovolemia ; Injections, Epidural ; Lidocaine ; Recurrence

OBJECTIVE: Human papillomavirus (HPV) has been identified more than 100 HPV subtypes. The distributions of subtypes are different according to nations and regions. We analysed subtype of infection with HPV among women who live in Pusan and surburbs of Pusan. We accessed the clinical usefulness of HPV DNA chip test as a supplementary method of Pap smear in the evaluation of cervical lesion. METHOD: This study was undertaken from January 2002 to January 2005 and the samples were collected from the patients who had abnormal Pap smear. We analysed subtypes of 143 positive cases with HPV DNA chip (Biomedlab) test and estimated pathologic reports of 115 patients except 28 patients who had not biopsy. We investigated pathologic results of 54 of 115 patients who had atypical squamous cells / low grade squamous intraepithelial lesion (ASC/LSIL) in Pap smear and examined high risk HPV in 54 pathologic results. RESULTS: The prevalence of HPV subtypes was 42 cases of HPV-16, 20 cases of HPV- 58, 16 cases of HPV-52, 10 cases of HPV-35, 9 cases of HPV-56, 7 cases of HPV-51, 6 cases of HPV-18 in descending order of incidence in high risk HPV group and 3 cases of HPV-6, 3 cases of HPV-42, 2 cases of HPV-34, 2 cases of HPV-43 in descending order of incidence in low risk HPV group. The results of HPV DNA chip test and 115 pathologic reports were estimated by comparative study. A pure infection with low risk HPV group was detected in low grade lesion. Infection with high risk HPV group was also detected in low grade lesion but was mainly detected in high grade lesion. The pathologic results of 54 patients who had ASC / LSIL in Pap smear were 13 patients had above high grade lesion include 2 cases of invasive carcinoma so false negative rate of Pap smear in the detection of high grade lesion was 24%. CONCLUSION: HPV subtypes were detected HPV 16, 58, 52, 35, 56, 51, 18 types in descending order of incidence and prevalence. Mass study and integrated data from larger population and various regions in many hospitals will be needed. And the supplementary use of HPV DNA chip test may provide clinical usefulness because it can reduce the false negative rate of Pap smear and improve the positive predictive value in the detection of high grade cervical lesion and it enables to decrease the incidence of cervical cancer.
Biopsy ; Busan ; Cervix Uteri* ; DNA* ; Female ; Human papillomavirus 16 ; Human papillomavirus 18 ; Human papillomavirus 6 ; Humans ; Incidence ; Oligonucleotide Array Sequence Analysis* ; Prevalence ; Uterine Cervical Neoplasms

No abstract available.
Hand

BACKGROUND: There has been a growing realization that a variety of anticancer drugs can induce apoptotic cell death. In the present study, an attempt was made to investigate the responsiveness of gastric cancer cells to various anticancer drugs and to identify which apoptosis-related proteins could be correlated to chemosensitivity. METHODS: Nine human Korean gastric cancer cell lines (SNU-1, -5, -16, -484, -601, -620, -638, -668, and -719) were analyzed. The cytotoxicity of each cell line to camptothecin, cisplatin, mitomycin C, vincristine, 5-FU, epirubicin, and doxorubicin was determined by using a MTT (dimethylthiazole- diphenyltetrazolium-bromide) assay. Apoptosis-related proteins (p53, p21, Bcl-2, Bcl-x, and Bax) were detected using a Western blot assay. RESULTS: Of the nine gastric cancer cell lines, SNU-1 was resistant while SNU-5 was sensitive to anticancer drugs. Mutated p53 was detected in all the cell lines. The highest expression of Bcl-2 was observed in SNU-1 while less or no expression of Bcl-2 was observed in SNU-5, -484, and -601. Bcl-xL was less expressed in SNU-5 than in the other cell lines. CONCLUSIONS: Chemosensitivity in gastric cancer cell lines was correlated mainly with the level of Bcl-2 and partly with that of Bcl-xL. There was no correlation between the chemosensitivity and other apoptosis-related proteins, such as p21, p53, Bax, and Bcl-xS in the studied gastric cancer cell lines.
Blotting, Western ; Camptothecin ; Cell Death ; Cell Line* ; Cisplatin ; Doxorubicin ; Epirubicin ; Fluorouracil ; Humans ; Mitomycin ; Stomach Neoplasms* ; Vincristine

The popularity of spinal anesthesia has decreased to some extent due to the widespread use of a balanced anesthesia technique, increasing interest in epidural anesthesia and fear of neurologic sequelae resulting from spinal anesthesia. The complication once most feared was adhesive arachnoiditis. However, in many study reports there were no instances of adhesive arachnoiditis, cauda equina syndrome or transverse myelitis. The majority of serious neurological symptoms appearing after spinal anesthesia can be attributed to coincidence or previously unrecognizaed disease. This is a case report in which the patient developed syringomyelia with chronic adhesive arachnoiditis. A 27-year-old patient, known to have adhesive arachnoiditis which developed after spinal anesthesia, was admitted to this institution for treatment of syringomyelia. He had history of a simple appendectomy done under spinal anesthesia on the 24th of November in 1976 and had experienced sensory changes and progressive motor weakness in the lower extremities 4 months after surgery. In January, 1978 he received an adhesiolysis operation after conservative therapy using steroid injections. Afterward, he developed syringomyelia resulting from adhesive arachnoiditis and had shunt surgery. He went home without significant improvement. However, it is unwise to make a final diagnosis and assume that the sequela was attributed only to the spinal anesthesia. It is important to seek other causes rather than to ascribe all to the spinal anesthesia because there are many other causative factors. In this case, it was not certain that adhesive arachnoiditis was a possible cause, but this could not be ruled out either. The prevention of complications and their causative factors must be carefully considered at all times in the practice of spinal anesthesia.
Adhesives ; Adult ; Anesthesia, Epidural ; Anesthesia, Spinal* ; Appendectomy ; Arachnoid* ; Arachnoiditis* ; Balanced Anesthesia ; Diagnosis ; Humans ; Lower Extremity ; Myelitis, Transverse ; Polyradiculopathy ; Syringomyelia*

PURPOSE: We prospectively evaluated whether the adequate age of surgery in patients with unilateral inguinal cryptorchidism is 1-2 years old. MATERIALS AND METHODS: Twenty-eight specimens were taken from ipsilateral undescended testicles in 28 unilateral inguinal cryptorchid boys(age range: 4-132 months, mean: 20.3 months). Patients were divided into 2 groups: an early group I of 9 cases(7-12 months) and a late group II of 19 cases(13-132 months). Control testicular biopsies were performed in 6 hydrocele boys(age range: 8-36 months, mean: 23 months). The histomorphological changes, including spermatogonia per tubule(S/T) value, Sertoli cell index(SCI), tubular degeneration phase V-VII(TDP V-VII), mean tubular diameter(MTD), and changes of peritubular interstitial tissue(thickened tubular basement membrane and peritubular fibrosis) were compared between the cryptorchidism and control groups and the adequate age of surgery in the cryptorchid child was evaluated. RESULTS: Testis volume, SCI, TDP V-VII, MTD, and changes of peritubular interstitial tissue, except S/T value, were significantly different between groups I and II(p<0.05). Between group I and the control group, there was a statistically significant difference in testis volume, MTD, and changes of peritubular interstitial tissue(p<0.05). CONCLUSIONS: These findings of decreased testis volume, SCI, MTD and increased peritubular fibrosis may suggest that the appropriate timing of surgery in the cryptorchid child is under 1 year.
Basement Membrane ; Biopsy ; Child ; Cryptorchidism* ; Fibrosis ; Humans ; Male ; Prospective Studies ; Spermatogonia ; Testis

No abstract available.
Ectopia Cordis* ; Hernia, Umbilical*