The purpose of the present study was to establish normal electroretinogram (ERG) parameters using 56 normal eyes of four dog breeds common in Thailand: poodle, Labrador retriever, Thai ridgeback, and Thai Bangkaew. Standard ERG findings were bilaterally recorded using a handheld multi-species ERG unit with an ERG-jet lens electrode for 28 dogs under preanesthesia with diazepam, anesthesia with propofol, and anesthesia maintenance with isoflurane. There were significant differences in the mean values of ERG amplitudes and implicit times among the four dog breeds (p < 0.05) except for the b-wave implicit time of the photopic 30 Hz flicker response with 3 cd.s/m2 (p = 0.610). Out of the four breeds, Thai Bangkaew had the longest implicit time (p < 0.001) of scotopic low intensity responses, b-wave of scotopic standard intensity responses (3 cd.s/m2), a-wave of the higher intensity response (10 cd.s/m2), and a-wave of the photopic single flash response (3 cd.s/m2). For the b/a ratio, only the ratio of the Cone response was significantly different among the different breeds. In this summary, normal ERG parameters for four dog breeds were reported. Data from the investigation supported the hypothesis that determination of breed-specific limits of normality for ERG responses is necessary for individual clinics and laboratories.
Animals ; Dogs/genetics/*physiology ; Electroretinography/veterinary ; Reference Values ; Retina/*physiology

PURPOSE: The most important function of the larynx is airway protection which is provided through a polysynaptic reflex closure triggered by the receptors in the glottic and supraglottic mucosa, evoking the reflex contraction of the laryngeal muscles especially by strong adduction of vocal cords. Based on the hypotheses that central facilitation is essential for this bilateral adductor reflex and that its disturbance can result in weakened laryngeal closure, we designed this study to elucidate the effect of central facilitation on this protective reflex. MATERIALS AND METHODS: Seven adult, 20 kg mongrel dogs underwent evoked response laryngeal electromyography under 0.5 to 1.0 MAC (minimum alveolar concentration) isoflurane anesthesia. The internal branch of the superior laryngeal nerve was stimulated through bipolar platinum-iridium electrodes, and recording electrodes were positioned in the ipsilateral and contralateral thyroarytenoid muscles. RESULTS: Ipsilateral reflex closure was consistantly recorded regardless of anesthetic levels. However, contralateral reflex responses disappeared as anesthetic levels were deepened. Additionally, late responses (R2) were detected in one animal at lower level of anesthesia. CONCLUSIONS: Deepened level of anesthesia affects central facilitation and results in the loss of the crossed adductor reflex, predisposing to a weakened glottic closure response. Precise understanding of this effect may possibly provide a way to prevent aspiration in unconscious patients.
Anesthesia/methods ; Animals ; Dogs ; Glottis/*physiology ; Laryngeal Nerves/physiology ; *Reflex

OBJECTIVETo determine the effect of the area postrema (AP) of the medulla oblongata on gastrointestinal interdigestive migrating motor complex (MMC) and the plasma motilin level.

METHODSInterdigestive MMC activities of the antrum and duodenum were recorded by strain gauge implanted on the serosa of 8 conscious dogs. A cannula was intubated in femoral vein for motilin injection. The plasma motilin concentration was measured by RIA. We observed: (1) normal interdigestive MMC activity and fluctuations in plasma motilin concentration; (2) the effects of electrically damaging the AP on MMC activity and plasma motilin level; (3) whether intravenous injection of motilin could induce phase III contractions after the AP was destroyed.

RESULTS(1) Typical interdigestive MMC with phase I, II, III, and IV was recorded in normal dogs. Phase III was concurrent with the peak of plasma motilin level. (2) In damaged AP dogs, antroduodenal interdigestive MMC contractions were suppressed; cyclic, phasic and migratory pattern of MMC was disrupted. Plasma motilin concentration was decreased. Intravenous injection of motilin could not induce phase III contractions.

CONCLUSIONSThe area postrema might play an important role, which is mediated by motilin, on the regulation of interdigestive MMC.

Animals ; Dogs ; Fourth Ventricle ; physiology ; Medulla Oblongata ; physiology ; Motilin ; blood ; Myoelectric Complex, Migrating ; physiology ; Stomach ; physiology

The optimal dark adaptation time of electroretinograms (ERG's) performed on conscious dogs were determined using a commercially available ERG unit with a contact lens electrode and a built-in light source (LED-electrode). The ERG recordings were performed on nine healthy Miniature Schnauzer dogs. The bilateral ERG's at seven different dark adaptation times at an intensity of 2.5 cd.s/m2 was performed. Signal averaging (4 flashes of light stimuli) was adopted to reduce electrophysiologic noise. As the dark adaptation time increased, a significant increase in the mean a-wave amplitudes was observed in comparison to base-line levels up to 10 min (p > 0.05). Thereafter, no significant differences in amplitude occured over the dark adaptation time. Moreover, at this time the mean amplitude was 60.30 +/- 18.47 microV. However, no significant changes were observed for the implicit times of the a-wave. The implicit times and amplitude of the b-wave increased significantly up to 20 min of dark adaptation (p > 0.05). Beyond this time, the mean b-wave amplitudes was 132.92 +/- 17.79 microV. The results of the present study demonstrate that, the optimal dark adaptation time when performing ERG's, should be at least 20 min in conscious Miniature Schnauzer dogs.
Animals ; Dark Adaptation/*physiology ; Dogs/*physiology ; Electroretinography/*veterinary ; Male ; Retina/*physiology ; Time Factors

Animals ; Dogs ; Duodenum ; physiology ; Male ; Myoelectric Complex, Migrating ; physiology ; Sphincter of Oddi ; physiology

The purpose of this study was to determine if salivary chromogranin a secretion in dogs exhibits a circadian rhythm. Saliva sampling was performed during three different sessions occurring in three nonconsecutive 24-h periods. Sixteen healthy adult beagle dogs (8 males and 8 females) were moved to a sampling room and housed individually in cages. Saliva samples were obtained every 4 h from 12:00 p.m. to 12:00 p.m. the following day. In the interest of habituation, saliva was obtained hourly from each dog 3 h before the experiment was started. Salivary chromogranin A concentrations were measured using an enzyme-linked immunosorbent assay. No circadian rhythm was detected for salivary chromogranin A secretion, and no differences in salivary chromogranin A concentrations measured every 4 h were demonstrated during the 24-h cycle in dogs.
Animals ; Chromogranin A/*analysis/*metabolism ; *Circadian Rhythm ; Dogs/*physiology ; Saliva/*chemistry

PURPOSE: Photoplethysmography (PPG) is a noninvasive optical technology that detects changes in blood volume in the vascular system. This study aimed to investigate the possibilities of monitoring the cardiovascular system status by using PPG. MATERIALS AND METHODS: Forced hemodynamic changes were induced using cardiac stimulants; dopamine and epinephrine, and PPG components were recorded by a noninvasive method at the peripheral blood vessels. The results were compared among 6 dogs. Endotracheal intubation was performed after an intramuscular injection of 25 mg/kg ketamine sulfate, and anesthesia was maintained with 2% enflurane. After stabilizing the animals for 15 min, 16 mg/mL diluted dopamine was injected into a vein for 2 min at 20 microgram/kg.min(-1) by using an infusion pump. Thereafter, the infusion pump was stopped, and 1 mg epinephrine was injected intravenously. Fluid administration was controlled to minimize preload change in blood pressure. RESULTS: After stimulant administration, systolic blood pressure (SBP) and diastolic blood pressures (DBP) increased. The direct current (DC) component, which reflects changes in blood volume, decreased while the alternating current (AC) component, which reflects changes in vascular compliance and resistance, increased. The correlation coefficient between SBP and the foot of the DC component was 0.939 (p < 0.01), while it was 0.942 (p < 0.01) for DBP and the peak of the DC component. The AC component could predict the increase in vascular resistance from a stable pulse blood volume, even with increased pulse pressure. Conclusions: These results support the possibility that PPG components may be used for easy and noninvasive measurement of hemodynamic changes in the cardiovascular system.
Animals ; Blood Pressure/*physiology ; *Blood Pressure Monitors ; Dogs/*physiology ; Humans ; Models, Animal ; Photoplethysmography/*methods

Sixty clinically normal German shepherd dogs, 31 males and 29 females, ranging in age from 1 and 5 years and with a body weight ranging from 22 to 37.2 kg, were examined by the two-dimensional mode, M-mode, and Doppler echocardiography. In Doppler mode, the mitral valve flows were obtained, where the aim was to determine the velocity peaks and ratios of the E and A waves and the mitral E wave deceleration time. The velocity peaks were obtained for the tricuspid, pulmonary and aortic valves. On the left ventricular outflow tract flow, the time velocity integral and aortic cross-sectional area was used to calculate the cardiac output. A statistically significant correlation with the body weight was found for the systolic left atrial and diastolic aortic diameter on twodimensional mode. On M-mode, there was a significant correlation between the body weight and the systolic left atrium and diastolic aortic dimension, systolic and diastolic left ventricular, septal and posterior wall dimensions. Doppler echocardiography showed that there was no significant correlation between the body weight and the mitral, tricuspid, pulmonary and aortic valves flows. These results demonstrate that it is important to know the normal echocardiographic values for German shepherd dogs because there are some characteristics peculiar to this particular breed. The data obtained is expected to be helpful for studies on small animal cardiology.
Animals ; Body Weight ; Dogs/*physiology ; Echocardiography/*veterinary ; Female ; Heart/anatomy&histology/*physiology ; Male ; Sex Characteristics

Thyroarytenoid(TA), lateral cricoarytenoid(LCA), and IA muscles are referred to as the adductors of the vocal fold. The TA is known to shorten the vocal folds and to adduct the membranous vocal fold, and the LCA adducts the inter-vocal process region and IA adducts the posterior commissure. Even though IA has an important role for the positioning of the vocal folds during respiration and phonation together with the action of the posterior cricoarytenoid muscle, little is known about the effect of IA on voice parameters during phonation. An in vivo canine model was used in five mongrel dogs to examine the role of the IA muscle in controlling phonation. In two out of five dogs, sound could not be elicited without stimulating the IA branches of the recurrent laryngeal nerves. When the IA was dynamically and statistically stimulated, subglottic pressure, vocal intensity and fundamental frequency were increased. However, open quotient was not changed markedly. These results suggest that the IA affects the voice parameters mainly by controlling subglottic pressure during phonation.
Animal ; Dogs ; Electric Stimulation ; Laryngeal Muscles/*physiology ; Laryngoscopy ; Models, Biological ; Phonation/*physiology ; Videotape Recording

OBJECTIVETo investigate electrophysiological characteristics of the coronary sinus musculature and explore its role on electrical conduction between left atrium and right atrium in isolated canine heart.

METHODThe electrical connections between coronary sinus, left atrium and right atrium were detected by programmed stimulation delivered at coronary sinus ostium, distal coronary sinus and lateral wall of left atrium in a Langendorff model of canine.

RESULTSDuring stimulation delivered at coronary sinus ostium and distal coronary sinus, posterior wall of left atrium was firstly activated by electronic signal through coronary sinus musculature, the conduct time of coronary sinus ostium stimulation and distal coronary sinus stimulation were (44 +/- 21) ms and (41 +/- 15) ms, respectively. During stimulation delivered at lateral wall of left atrium, electronic signal was firstly observed in coronary sinus. During premature stimulation, conduction blockade of coronary sinus to left atrium was evidenced in parts of hearts, but electronic conduction of left atrium to right atrium remained stable. The ERP at the different stimulation sites of coronary sinus ostium and distal coronary sinus, posterior wall of left atrium were (122 +/- 19) ms, (114 +/- 12) ms (n = 3) and (107 +/- 17) ms (all P > 0.05).

CONCLUSIONA conduction way connecting left and right atria exists in coronary sinus which might play an important role on inducing and maintaining atrial arrhythmias under certain pathological conditions.

Animals ; Coronary Sinus ; physiology ; Dogs ; Heart Atria ; Heart Conduction System ; physiology ; Male ; Myocardium