Healthy human serum IgA, purified lectin-sepharose 4B column affinity chromatography was assessed by sodium dodecyl sulfate polyacrylamide electrophresis. The lectin from wild Jackfruit reacted specifically to IgA1 from human serum. This lectin used as antigen in the technique of ELISA-Lectin to determine the concentration of IgA1 from healthy and pathological human sera. Using protein A from Staphylococcus aureus isolated from Vietnamese infectious patients to capture IgG average concentration of IgA1 from healthy human serum as 14.37 mg/ml. No change of IgG concentration from patients sera (hepatoma and leukemia) was obtained in comparison with healthy subjects
Carcinoma, Hepatocellular ; Enzyme-Linked Immunosorbent Assay ; Leukemia ;