Complete decompression is very important for the surgical treatment of the spinal stenosis. But it may produce the segmental instability or spondylolisthesis as postoperative complications. So rigid internal fixation is mandatory to reduce this problem. Authors treated 16 cases of spinal stenosis by complete decompression and pedicale screw fixation and posterolateral fusion, analyzed 11 cases which were followed more than 9 months. The results were as follows: 1. Among the 11 cases, the mean age was 49 years old and there were 5 males and 6 females. 2. The most common cause was degenerative type, 7 cases. The most frequent level of involvement was L4–L5, 10 cases and two level involvement was 3 cases. 3. Among the 11 cases, 6 cases were excellent and 4 cases were good, and 1 case was fair, in Hanley's criteria. 4. In spondylolisthetic stenosis, the average percentage of slippage was changed from 22% pre-operatively to 2.7% postoperatively and 3.8% after 9 months follow up. 5. The complications were relatively few(1 case of hematoma and 3 cases of pain on the bone graft donor site).
Constriction, Pathologic ; Decompression ; Female ; Follow-Up Studies ; Hematoma ; Humans ; Male ; Pedicle Screws ; Postoperative Complications ; Spinal Stenosis ; Spondylolisthesis ; Tissue Donors ; Transplants

OBJECTIVE: The purpose of this study was to determine the normal value of cervical motion in Korean adults. METHOD: Five hundred healthy subjects, 250 men and 250 women between third decades and seventh decades completed this study. The Cervical Range of Motion (CROM) instrument was used to measure six cervical motions and horizontal head motion. RESULTS: The elderly group had significantly less motion than the younger group for all six motions and horizontal head motions (p<0.05). Women had greater range of cervical motions than men in all cervical motions (p<0.05). CONCLUSION: The method using the CROM instrument seems to be useful for primary and follow up test of cervical disease.
Adult* ; Aged ; Female ; Head ; Humans ; Male ; Range of Motion, Articular* ; Reference Values

The talus is a bone with unique biomechanical features and vascular supply. Although fractures of the talus are not common, the complications of the displaced fractures or dislocation are frequent and resulting disabilities are so severe that the importance of proper management is emphasized. Authors analyzed 17 cases of fracture-dislocation of the talus, treated at Soon Chun Hyang University Hospital from January 1983 to December 1988. The results were as follows:l. Among the 17 cases, there were 15 males and 2 females and the average age was 28 years old, ranged from 21 years to 58 years. 2. The causes were fall from height in 10 cases, traffic accident in 6 and others in one. 3. According to Marti-Weber classification, 2 cases were type I, 5 in type II, 7 in type III and 3 type IV. 4. Among the 17 cases, 9 cases in type I and II were treated conservatively, and 8 cases in thpe III and IV were treated operatively. 5. Complications were avascular necrosis in 3 cases, degenerative arthritis in 4 and nonunion in 1. 6. Final results, evaluated by the criteria of Mindell et al., were as follows: excellent in 7 cases, good in 5, fair in 3 and poor in 2.
Accidents, Traffic ; Classification ; Clinical Study ; Dislocations ; Female ; Humans ; Male ; Necrosis ; Osteoarthritis ; Talus

OBJECTIVE: The purpose of this study was to examine the change of heart rate, mean arterial pressure, respiration rate, and spasticity at passive leg range of motion exercise for one month in hemiplegic patients. METHOD: Subjects were composed of twenty hemiplegic patients who had spasticity above two grade in modified Ashworth scale. Passive leg range of motion exercise was performed with passive cycle leg exerciser (Autocybex) at the speed of 40 rpm. Training program consisted of two sessions a day, and each session was done for twenty minutes. The heart rate, mean arterial pressure, and respiration rate were checked for each subject before and after passive leg range of motion exercise at initial state and after one month. Spasticity was measured at knee joint of the affected side at initial state and after one month. RESULTS: The resting heart rate, mean arterial pressure and respiration rate were significantly decreased after one month (p<0.05). The changes of heart rate, mean arterial pressure, and respiration rate after passive leg range of motion exercise were significantly decreased after one month (p<0.05). The grade of spasticity was decreased after one month (p<0.05). CONCLUSION: We concluded that passive leg range of motion exercise during one month stabilized heart rate, mean arterial pressure, and respiration rate, and reduced spasticity.
Arterial Pressure ; Education ; Heart Rate ; Humans ; Knee Joint ; Leg* ; Muscle Spasticity ; Range of Motion, Articular* ; Respiratory Rate

OBJECTIVE: The purpose of this study was to examine the changes of cardiopulmonary response and serum lipid level after backward walking training in healthy male college students. METHOD: Subjects were ten healthy male college students. Maximal heart rate and VO2max were determined for each subject by administering a Bruce treadmill exercise test after backward walking training during eight weeks. Wilcoxon signed rank test was used to evaluate the changes of the backward walk training. RESULTS: The study showed that maximal heart rates were 163.7 beat/min in pre-training and 175.8 beats/min in post- training; in contrast, resting heart rates were 72 beat/min in pre-training and 67 beat/min in post-training. The maximal oxygen consumptions were 2111 ml/min in pre-training and 2521 ml/min in post-training. There were significant differences (p<0.05) in maximal heart rate, heart rate ratio of maximal heart rate (% HR), oxygen consumption, and oxygen consumption ratio of maximal oxygen consumption (% VO2max) between the pre and post-training. The HDL cholesterol level was 42.8 mg/dl in pre-training and 58.0 mg/dl in post-training. There was significant increased of HDL cholesterol in post-training. CONCLUSION: We concluded that 5 km/hr backward walk training for eight weeks was an effective exercise program to increase in aerobic capacity of the male college students.
Cholesterol, HDL ; Exercise Test ; Heart Rate ; Humans ; Male ; Oxygen Consumption ; Walking

We treated 9 patients of ventrally or ventrolaterally located thoracic lesions with lateral extracavitary approach, six cases of ventrally located thoracic cord tumor, and three cases of central disc herniation. After operation, the patients' neurological symptoms were effectively improved and there were no signigicant postoperative c omplications. Compared to transthoracic approach, this approach is less invasive and enable simultaneous vertebral reconstruction and posterior spinal fixation. Although relatatively small in number experienced, lateral extracavitry approach is considered to be a alternative method to transthoracic approach for the treatment of ventrally and ventrolaterally located thoracic lesion.
Humans

PURPOSE: In this study, we evaluated the effects of glucose on the proliferation, migration, morphological characteristics, and proteinase expressions in human corneal epithelial cells to discover the cause of diabetic corneal epithelial complications. METHODS: Human corneal epithelial cells transfected by SV40 were cultured in media containing 17.5 mM (control), 25 mM, and 100 mM D-glucose to stimulate a diabetic condition. In some experiments, 25 mM or 100mM D-mannitol was added to the media to control for the osmotic effect of glucose. We performed MTT analysis of the proliferation assay, cell count of the migration assay, scanning electron microscopic (SEM) examination for morphologic change, and RT-PCR for the expression of mRNA of metalloproteinase (MMP). RESULTS: Raising the concentration of glucose from 25 mM to 100 mM significantly decreased in the cellular proliferation and migration. These findings were not observed in the presence of 25 mM or 100 mM mannitol. In SEM, the pseudopods of the cells were markedly decreased in the 100mM glucose media. Although the expression of MMP-2 was not significantly different, that of MMP-9 was significantly increased with increasing glucose concentration. CONCLUSIONS: Higher levels of glucose show a significant effect on cellular proliferation, migration, morphology, and the expression of MMP-9 in corneal epithelial cells. This study may be an initial step in understanding the mechanism of corneal epithelial wound healing in diabetic patients.
Cell Count ; Cell Proliferation ; Epithelial Cells* ; Glucose* ; Humans* ; Mannitol ; RNA, Messenger ; Wound Healing

A hippocalcin cDNA from rat brain cDNA library was amplified by polymerase chain reation(PCR) and cloned using TA Cloning technique. For this PCR cloning, 29mer and 28mer oligonucleotide primers containing BamHl and EcoRl sites at the 5' end and 3' end, respectively were used. The nucleotide sequence of hippocalcin cDNA c1one was determined, and the complete amino acid sequence was deduced. Recombinant clone contained a cDNA insert of 610 base pairs with 582 nucleotides of open reading frame including the temination codon, 23 nucleotide of 5'-untranslated region, and 5nucleotides of 3'-nutran,slated region. The open reading frame encoded a polypepetid comprising 193 amino acids with molecular weight of 22kDa. The cDNA insert was subcloned into pVLI393 Baculovirus transfer vector. The recombinant hippocalcin was expressed in insect cell(Sf9 cell) using expression vector pVL1393. The hippocalcin expressed was purified as a single band on polyacrylamide gel electrophoresis following hydrophobic phenyl HPLC and TSKgel G3000SW gel filtration HPLC. Molecular size of rat brain hippocalcin protein expressed in this system was estimated to be 22kDa. Myristoylated hippocalcin migrated faster than nonmyristoyated form on SDS-polyacrylamide gel. Less than 10% of total hippocalcin expressed was myristoylated in this baculovirus expression system.
Amino Acid Sequence ; Amino Acids ; Animals ; Baculoviridae ; Base Pairing ; Base Sequence ; Brain* ; Chromatography, Gel ; Chromatography, High Pressure Liquid ; Clone Cells ; Cloning, Organism ; Codon ; DNA Primers ; DNA, Complementary* ; Electrophoresis, Polyacrylamide Gel ; Gene Library ; Hippocalcin* ; Insects ; Molecular Weight ; Nucleotides ; Open Reading Frames ; Polymerase Chain Reaction ; Rats*

BACKGROUND: Extended-spectrum cephalosporins and fluoroquinolones are important antimicrobials for treating invasive salmonellosis, and emerging resistance to these antimicrobials is of paramount concern. METHODS: A total of 30 Salmonella spp. clinical isolates recovered in Gyeongsangbuk-do from 2012 to 2013 were characterized using antibiotic resistance profiles and pulsed-field gel electrophoresis (PFGE). RESULTS: A high prevalence of multidrug-resistant isolates, mainly showing an ampicillin, nalidixic acid, chloramphenicol resistance pattern, was observed. Four extended-spectrum beta-lactamase (ESBL)-producing isolates (3 CTX-M-15 isolates and 1 CTX-M-27 isolate) were found. The bla(CTX-M-27) gene was carried by an IncF conjugative plasmid in the S. Infantis isolate. The bla(CTX-M-15) gene were carried by an IncF (2 isolates) or IncHI2 (1 isolate) conjugative plasmid in S. Enteritidis. In addition, a single mutation of GyrA, Ser83Thr (1 isolates), Asp87Tyr (9 isolates), Asp87Gly (4 isolates), and Asp87Leu (3 isolates), was detected in nalidixic acid-resistant Salmonella spp. isolates. XbaI PFGE analysis of all isolates revealed more than 19 different pulsotypes. The most common S. Enteritidis PFGE pattern (SEGX01.003) was associated with a larger number of cases of invasive salmonellosis than all other patterns. CONCLUSION: The information from our study can assist in source attribution, outbreak investigations, and tailoring of interventions to maximize disease prevention.
Ampicillin ; beta-Lactamases ; Cephalosporins ; Chloramphenicol Resistance ; Drug Resistance ; Drug Resistance, Microbial ; Electrophoresis, Gel, Pulsed-Field ; Fluoroquinolones ; Gyeongsangbuk-do ; Nalidixic Acid ; Plasmids ; Prevalence ; Salmonella Infections ; Salmonella*

PURPOSE: This study was performed to determine the role of nuclear factor kappa B (NF-kappa B) on the lens epithelial cell death after ultraviolet (UV) irradiation. METHODS: Simian virus 40 transfected human lens epithelial cells (HLE B-3 cells) were used in this study. UVB located at 10cm from the bottom was irradiated during 1, 2, 3 and 4 minutes. To measure the cytotoxicity MTT assay was used. Translocation of NF-kappa B was examined by immunocytochemistry with anti NF-kappa B p65 antibody and electrophoretic mobility shift assay (EMSA). To confirm the role of NF-kappa B, the cells were pretreated with sulfasalazine, a specific inhibitor of NF-kappa B, for 30 minutes before irradiation, and cytotoxicity and translocation of NF-kappa B were evaluated. RESULTS: UV irradiation produced a progressive cytotoxic effect in cultured HLE B-3 cells after 1 minute and maximum cytotoxicity was reached after 3 minutes irradiation. When HLE B-3 cells were irradiated with UVB, the translocation of NF-kappa B was observed in immunocytochemistry. These translocations were peaked 6 hours after UV irradiation in EMSA. In HLE B-3 cells pretreated with sulfasalazine, the translocation of NF-kappa B was blocked. The cellular death after UV irradiation was markedly blocked by sulfasalazine. UV irradiation can translocate NF-kappa B and sulfasalazine is a useful blocking agent in this pathway. In addition, sulfasalazine can prevent cellular death after UV irradiation. CONCLUSIONS: These findings suggest that NF-kappa B plays an important role in cellular death after UV irradiation.
Electrophoretic Mobility Shift Assay ; Epithelial Cells* ; Humans ; Immunohistochemistry ; NF-kappa B* ; Simian virus 40 ; Sulfasalazine ; Transcription Factor RelA