We studied the biological effects of Mitomycin C(MMC) in cellular metabolic activity and cytotoxicity on the corneal epithelial cell in rabbit that is clinically used according to MMC concentrations and exposure time. The concentration of MMC were 0.01%, 0.02%, 0.04%, 0.06%, 0.1%, and exposure time were 5, 10, and 30 minutes. MTT assay (3-I4,5-Dimethy-lthiazol-2-ylI-2,5-diphenyltetrazolium bromide) and transmission electromicroscopic examination were performed to access the metabolic activity of corneal cell, toxicity of corneal epithelial cell, and cellular morphology. In calorimetric assay, the higher the concentration of MMC and longer the duration of exposure time, the cellular metabolic activity was decreased. Also the toxicity of corneal epithelial cell was increased in a concentration and time dependent manner. The metabolic activity of corneal cell were inhibited by 50% at least over MMC 0.04% for 30 minutes exposure time. The LD50 at 5 minutes exposure was MMC 0.057%. In histologic finding, the higher the concentration of MMC, the change of cellular structure such as loss of microvilli, enlargement of rough endoplasmic reticulum or mitochondria, damage of nuclear membrane were more appeared. To obtain the efficacy of MMC for inhibition of cellular proliferation, we think that further experimental and clinical studies should be need to assess the safe concentration and exposure time of MMC to the corneal toxicity.
Cell Proliferation ; Cellular Structures ; Endoplasmic Reticulum, Rough ; Epithelial Cells ; Lethal Dose 50 ; Microvilli ; Mitochondria ; Mitomycin ; Nuclear Envelope

No abstract available.
Female ; Follicle Stimulating Hormone* ; Gonadotropin-Releasing Hormone* ; Gonadotropins* ; Humans ; Ovarian Diseases*

No abstract available.
Female ; Methotrexate* ; Pregnancy ; Pregnancy, Tubal*

No abstract available.
Contraceptives, Oral* ; Fertilization in Vitro* ; Gonadotropins*

OBJECTIVES: Nasal continuous positive airway pressure (nCPAP) is the treatment of choice and has been shown to reduce the frequency of nocturnal respiratory events, improve sleep architecture, and decrease daytime sleepiness in patients with obstructive sleep apnea syndrome (OSAS). However, little is known about the compliance of nCPAP treatment in Korea. Our objective was to look into the nCPAP compliance and examine the factors influencing it. METHODS: We reviewed the records of one hundred and twenty consecutive patients with OSAS referred for nocturnal polysomnography with nCPAP pressure titration during the period of January 1995 through April 1999 to the Seoul National University Hospital. We performed a telephone interview and obtained data from eighty-three patients. RESULTS: In sixty patients who had accepted nCPAP treatment, twenty-six patients (43.3%) were still using nCPAP device, while thirty-four patients (56.7%) stopped using it. Fifteen patients (25%) were using nCPAP device everyday. In thirty-four patients who discontinued nCPAP use, twenty-five patients (73.5%) did within the first three months, and thirty-one (91.2%) within the first year. Significant predictor of long-term nCPAP use was the presence of subjective daytime sleepiness before nCPAP application. CONCLUSIONS: Long-term compliance with nCPAP treatment appears to be associated with the presence of subjective daytime sleepiness before nCPAP application. Long-term compliance with nCPAP may be mostly predicted from the usage pattern within the first three months of use.
Compliance* ; Continuous Positive Airway Pressure* ; Humans ; Interviews as Topic ; Korea ; Polysomnography ; Seoul ; Sleep Apnea, Obstructive*

Better palliative management of patients with disseminated cancer has prolonged their lives but has raised the incidence of pathologic fractures in their variable lives. The essence of treatment is to prolong life, maintain comfort, and stabilize the patient with metastatic disease. The treatment of pathologic fractures with internal fixation has long been accepted by orthopedic surgeons and the benefits afforded to the patient are well known, so adequate fixation is essential. A combination of methylmethacrylate and internal fixation devices can greatly enhance the stability of fixation, because diseased bone is a poor base on which to insert an internal fixation device. The authors have treated 19 cases of the pathologic fracture of spine, humerus and femur by use of bone cement with and without internal fixation devices. The results are as follwed. 1. All cases utilized methylmethacrylate and there was no complication in methylmethacrylate in itself. 2. All cases had received various type of ancillary treatment: radiation in 9 cases, chemotherapy in 4 cases and in 8 cases, conservative pain control. 3. In spinal pathologic fracture with paraplegia or neurologic deficiency, the patients could walk with walker at an average of two weeks after operation. 4. Among the 19 cases, the pain was relieved markedly and ambulation was possible easily and early after operation in 16 cases. 5. Among the 19 cases, two survived less than 2 months, 7 surrived from 2 month to 3 month, five survived from 3 months to 6 months, four survived from 6 months to a year and one survived more than a year.
Drug Therapy ; Femur ; Fractures, Spontaneous ; Humans ; Humerus ; Incidence ; Internal Fixators ; Methylmethacrylate ; Orthopedics ; Paraplegia ; Spine ; Surgeons ; Walkers ; Walking

A total 73 cases of primary malignant bone tumors was reviewed and analysed clinically at the department of orthopaedic surgery, Kosin medicsl center, Pusan, Kores for 11 years from January, 1975 to December, 1985. The results were obtained as follows ; l. In the 73 cases of primsry malignant bone tumors, osteogenic sarcoma was the most common primary malignant bone tumor (57%) and followed by chondrossrcoma (10%), multiple myeloma (8%). 2. Average survival times according to each primary malignant bone tumors was more than 3 years in chondrosarcoma, reticulum cell sarcoma, and synovial sarcoma, 28 months in osteogenic sarcoma, and 7 months in Ewings sarcoma. Ewings sarcoma had the worse prognosis and the slowly progressing tumors-chondrosarcoma, reticulum cell sarcoma and synovial sarcoma are needed long term follow up. 3. In osteogenic sarcoma, the prognosis was better when developed in their 3rd decsde than when developed in their 2nd decade. 4. There is a slight difference in average survival time on the location of the site, for example when tumor is located in the distal femur, the prognosis was worst. 5. There is no difference in the prognosis. The mode of treatment did not effect to their prognosis. 6. It was clear that the tumors which had not been responded to chemotherapy or radiation therapy had poorer prognosis. Chondrosarcoma, fibrosarcoma and synovisl sarcoma were considered as slowly progressed tumors, and so it may be benefit to the patients that chemotherapy and/or radiotherapy were prescribed.
Busan ; Chondrosarcoma ; Drug Therapy ; Femur ; Fibrosarcoma ; Follow-Up Studies ; Humans ; Lymphoma, Non-Hodgkin ; Multiple Myeloma ; Osteosarcoma ; Prognosis ; Radiotherapy ; Sarcoma ; Sarcoma, Ewing ; Sarcoma, Synovial

Acquired port-wine stain is rare. It mimics a congenital port-wine stain morphologically but the pattern of onset is acquired after birth. It represents a progressive ectasia of vessels located in the superficial vascular plexus. The exact mechanism is unknown but some reported cases have occurred after trauma. We herein report five cases of acquired port-wine stains and they were idiopathic in nature.
Dilatation, Pathologic ; Parturition ; Port-Wine Stain*

No abstract available.
Pre-Eclampsia*

No abstract available.
Hair ; Survival Rate ; Transplants