1.Using cell membrane chromatography and HPLC-TOF/MS method for in vivo study of active components from roots of Aconitum carmichaeli
Yan CAO ; Xiaofei CHEN ; Diya Lü ; Xin DONG ; Guoqing ZHANG ; Yifeng CHAI
Journal of Pharmaceutical Analysis 2011;01(2):125-134
An offline two-dimensional system combining a rat cardiac muscle cell membrane chromatography time-of-flight mass spectrometry (CMC-TOF/MS) with a high performance liquid chromatography time-of-flight mass spectrometry (HPLC-TOF/MS) was established for investigating the parent components and metabolites in rat urine samples after administration of the roots of Aconitum carmichaeli.On the basis of the analysis of the first dimension,retention components of the mine sample were collected into 30 fractions (one fraction per minute).Then offline analysis of the second dimension was carried out.34 compounds including 24 parent alkaloids and 10 potential metabolites were identified from the dosed rat urine,and then binding affinities of different compounds on cell membranes were compared and influences of some functional groups on activity were estimated with the semi-quantification and curve fitting method.As a result,binding affinities decreased along with the process of deacylation,debenzoylation and demethylation,which may be related to the alleviation of toxicity in the procedure of herb processing or metabolism.Moreover,some minor components in rat urine (Songorine,14-benzoylneoline,Deoxyaconitine,etc.) exerted relatively strong affinity on cell membranes are worth exploring.The results delivered by the system suggest that the CMC can be applied to in vivo study.
2.Comparative analysis of essential oils found in Rhizomes Curcumae and Radix Curcumae by gas chromatography-mass spectrometry
Diya Lü ; Yan CAO ; Ling LI ; Zhenyu ZHU ; Xin DONG ; Hai ZHANG ; Yifeng CHAI ; Ziyang LOU
Journal of Pharmaceutical Analysis 2011;01(3):203-207
A comparison of the volatile compounds in Rhizomes Curcumae (Ezhu) and Radix Curcumae (Yujin) was undertaken using gas chromatography-mass spectrometry (GC-MS).Ultrasonic extraction and GC-MS methods were developed for the simultaneous determination of five sesquiterpenes,namely,α-pinene,β-elemene,curcumol,germacrone and curdione,in Ezhu and Yunjin.Good linearity (r>0.999) and high inter-day precision were observed over the investigated concentration ranges.The validated method was successfully used for the simultaneous determination of five sesquiterpenes in Ezhu and Yujin.The quantitative method can be effectively used to evaluate and monitor the quality of Chinese curcuma in clinical use.
3.Advances in the mechanism of Traditional Chinese Medicine by network pharma-cology method
Xinrui XING ; Diya LÜ ; Yifeng CHAI ; Zhenyu ZHU
Journal of Pharmaceutical Practice 2018;36(2):97-102
Network pharmacology is the basis of system biology,systematically expounded the interaction of the princi-ple and regulation between body and the drug,representing the idea of the modern biomedical philosophy and researching mod-el.It could be more easily understand the mechanism of action of Traditional Chinese Medicine treatment of diseases by the net-work pharmacology-related technical means.Network pharmacology is widely used in Traditional Chinese Medicine research in the current.The application and development of network pharmacology in the field of Traditional Chinese Medicine research in recent years were introduced in this paper from the aspects of Traditional Chinese Medicine concept,network pharmacology and network pharmacology application.
4.Application of surface plasmon resonance biosensor in quantitative analysis re-search
Langdong CHEN ; Zhongyun DONG ; Diya LÜ ; Zhenyu ZHU ; Bin LU ; Yan CAO
Journal of Pharmaceutical Practice 2018;36(1):18-23
Surface plasmon resonance(SPR) is an optical phenomenon arises from mass changes on sensors based on in-teraction between substances ,which could monitor the interaction between biomolecules quickly and accurately .It has the ad-vantage of label-free ,high specificity ,high accuracy ,real-time and on-line monitoring ,which has attracted comprehensive at-tention in recent years .SPR biosensors could be applied in drug discovery ,clinical diagnosis ,food safety ,environment monito-ring and proteomics .In this manuscript ,the application of SPR biosensors in food safety ,environment monitor and biomedical analysis have been reviewed ,which could provide reference to related research .