Objective To analyze the etiological factor for hypo-side agglutination in cross match blood test(CMT)with microcolumn gel assay,and to provide a guide to the clinical blood transfusion.Methods The data were collected and analyzed about 81 cases with hypo-side agglutination in CMT with microcolumn gel assay and direct antiglobulin test(DAT)positive from Jan.2007 to Oct.2008.Results Among the 81 hype-side agglutinated cases,most were with kidney disease,liver and gall disease,hematologic disease and immunologic disease.Specially,the kidney disease was most,accounting for 16.2%.Conclusion The analysis contributes to disposal in CMT and the safety of clinical blood transfusion.