1.Oxidative refolding of proteins.
Chinese Journal of Biotechnology 2003;19(1):1-8
The mechanism of oxidative refolding of proteins was elucidated in more detail from the intensive and extensive studies in the past decades. 1. Most of the proteins examined so far proceed oxidative refolding via multiple pathways rather than a single and specific pathway. This is consistent with the folding energy landscape theory. 2. It is the native interactions rather than the non-native interactions that direct the folding process. This is not necessarily incompatible with the importance of the non-native disulfide intermediates in the bovine pancreatic trypsin inhibitor (BPTI) pathway, which are just a chemical necessity in the intramolecular arrangement to facilitate native disulfide formation. 3. Based on the BPTI refolding it was suggested that disulfide bonds have a stabilizing effect on the native state without determining either the folding pathway or the final three-dimensional structure of the protein. This point of view is not applicable to other proteins. Studies on the refolding of prochymosin unequivocally demonstrated that the formation of native disulfides is the prerequisite to the recovery of the native conformation. It is more likely that the interdependence between the native disulfide formation and the formation of native structure is a general rule. 4. At the early stage of oxidative refolding disulfide formation is essentially a random process, with the progress of refolding further disulfide formation is increasingly dependent on the conformations of the intermediates. Enhancing the renaturation yield of recombinant proteins is a major challenge in biotechnology. In addition to aggregation, the formation of species with mispaired disulfide bonds is a leading cause of decreased yield. Progress in understanding the mechanism of oxidative refolding has provided insight into how to solve this problem. As described above, at the later stage of refolding disulfide formation depends on the conformations of intermediates. The intermediates with native-like and flexible structure favourable for native disulfide formation and correct refolding are productive intermediates, while the unproductive intermediates tend to adopt stable conformations, which render the thiol groups and disulfide bond(s) inaccessible and further folding unfavourable energetically. Therefore, the principle to enhance the renaturation yield of disulfide-containing proteins is to cause the productive intermediates to predominate by destabilizing the unproductive intermediates. To approach this, alkaline pH, low temperature, labilizing agents, protein disulfide isomerase and its analogues and alteration of primary structure have been proved useful to adjusting the structure of the unproductive intermediates so as to facilitate thiol/disulfide interchange and in turn the native disulfide formation. The prospects for the oxidative refolding of proteins both in basic and applied researches are discussed in this review article.
Animals
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Biotechnology
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Disulfides
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chemistry
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Humans
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Oxidation-Reduction
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Protein Folding
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Proteins
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chemistry
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genetics
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metabolism
2.Protein crystal quality oriented disulfide bond engineering.
Mengchen PU ; Zhijie XU ; Yao PENG ; Yaguang HOU ; Dongsheng LIU ; Yang WANG ; Haiguang LIU ; Gaojie SONG ; Zhi-Jie LIU
Protein & Cell 2018;9(7):659-663
3.Improved biochemical characteristics of human disulfide-stabilized Fv fragment to rabies virus.
Xiao-Ling ZHAO ; Jun YIN ; Su-Juan ZHANG ; Wei-Qiang CHEN
Chinese Journal of Biotechnology 2005;21(2):265-269
This study is (1) to improve the stabilization of human scFv to rabies virus; (2) to prepare active human dsFv fragment; and (3) to evaluate the biological activities of dsFv. The dsFv V(H) and VL were separately expressed in PET22b(+)/BL21 (DE3), solublized and combined in appropriate molar ratio in refolding solution. The resultant dsFv fragments were evaluated for its protection against rabies virus, its affinity and stability, in reference to the cognate scFv. The dsFv was found to bind specifically to Vero vaccine of rabies virus. Compared to the scFv, the dsFv was more stable, had higher affinity, and was able to inhibit the infection of Rabies virus to Vero cell. This established a solid basis for the clinical application of dsFv to rabies virus.
Antibodies, Viral
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immunology
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Disulfides
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chemistry
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Humans
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Immunoglobulin Fragments
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immunology
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metabolism
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Immunoglobulin Variable Region
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immunology
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metabolism
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Rabies virus
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immunology
4.Mechanism of Fas/FasL signal transduction pathway in K562 cell apoptosis induced by diallyl disulfide.
Xiao-Cheng YIN ; Yan-Hui PENG ; Zheng-Xiang XIAO
Journal of Experimental Hematology 2011;19(3):634-637
The aim of this study was to investigate the effect of diallyl disulfide (DADS) on the apoptosis of K562 cells and to explore the mechanism of K562 apoptosis induced by DADS. The K562 cells were treated with different concentrations of DADS for 24, 48 and 72 hours. The concentrations of DADS were as follows: 0 (control group), 10, 20, 40 and 80 mg/L. The morphologic changes of leukemia K562 cells treated with DADS were observed by Hoechst33 258 staining. The apoptosis of K562 cells treated with different concentrations of DADS for 24, 48 and 72 hours was analyzed by flow cytometry. The mRNA expression changes of Fas and FasL were detected by reverse transcription-polymerase chain reaction (RT-PCR) after K562 cells were treated with different concentrations of DADS for 48 hours. The results indicated that the characteristics of apoptosis in K562 cells induced by DADS were as follows: reduction of nucleus, chromatin condensation and nuclear membrane rupture. The flow cytometry with PI straining showed that after 24 hours of DADS treatment the apoptosis rate of K562 cells increased from 11.60 ± 0.83% at the concentration of 10 mg/L to 37.94 ± 0.87% at the concentration of 40 mg/L. The apoptosis rate of K562 cells increased from 37.94 ± 0.87% (24 hours) to 47.02 ± 0.66% (72 hours) after treatment with DADS of 10 mg/L increasing to 40 mg/L DADS. The Fas mRNA expression levels of the related apoptotic genes increased after K562 cells were treated with different concentrations of DADS for 48 hours, while FasL mRNA expression decreased significantly after DADS treatment for 48 hours, compared with those in the control group (p < 0.05). It is concluded that DADS can induce the apoptosis of human leukemia K562 cells in a time-and concentration-dependent manners. The activation of Fas/FasL pathway may play an important role in the K562 cell apoptosis induced by DADS, which is associated with increasing Fas gene expression and decreasing FasL gene expression.
Allyl Compounds
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pharmacology
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Apoptosis
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drug effects
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Disulfides
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pharmacology
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Fas Ligand Protein
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metabolism
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Gene Expression Regulation, Leukemic
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Humans
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K562 Cells
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Signal Transduction
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fas Receptor
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metabolism
5.Tissue distribution of solid lipid nanoparticles loaded garlic oil in rats.
Xue-hui SUN ; Tao GUO ; Jin HE ; Ming-hong ZHAO ; Shu-fang NIE
China Journal of Chinese Materia Medica 2008;33(23):2772-2775
OBJECTIVETo investigate the tissue distribution of the diallyl disulfide (DADS) and diallyl trisulfide (DATS) in solid lipid nanoparticles loaded garlic oil (GO-SLN) in rats.
METHODThe gas chromatography-electron capture detection (GC-ECD) method was established to determined the DADS and DATS simultaneously in the biological samples of rats after administration of 0.5 mL garlic oil injection or GO-SLN (containing about 10 mg garlic oil) via jugular vein cannula. The conditions for gas chromatographic separation were as follows. The oven temperature was set at 110 degrees C and maintained for 15 min. Temperatures at the injection port and detector were 180 degrees C and 300 degrees C, respectively. Ultra-pure nitrogen (purity > 99.999%, Shenyang Kerui Special Gases Co. Ltd., China) was used as a carrier gas and made-up gas at flow-rates of 1 mL x min(-1) and 60 mL x min(-1), respectively. All injections were carried out in the split injection mode with a split ratio of 1:10.
RESULTThe GC-ECD method was fit for determing the concentration of DADS and DATS in garlic oil. The distribution character of GO-SLN in rats had changed to some extent and the concentration of GO-SLN in tissues was higher than that of GO-Injection.
CONCLUSIONThe SLN can elevate the passive targeting of drugs and lengthen their action time in tissues.
Allyl Compounds ; analysis ; pharmacokinetics ; Animals ; Disulfides ; analysis ; pharmacokinetics ; Female ; Garlic ; chemistry ; Male ; Nanoparticles ; administration & dosage ; chemistry ; Plant Oils ; administration & dosage ; chemistry ; pharmacokinetics ; Rats ; Rats, Wistar ; Sulfides ; analysis ; pharmacokinetics
6.Effect of diallyl disulfide on learning and memory abilities and hippocampal synapses in mouse models of Alzheimer's disease.
Ji-Xia CHAI ; Hui-Hui LI ; Yuan-Yuan WANG ; Qiang CHAI ; Wen-Xin HE ; Yan-Mei ZHOU ; Xiao-Dong HU ; Zhen-Huan WANG
Journal of Southern Medical University 2016;36(10):1417-1422
OBJECTIVETo explore the effect of diallyl disulfide (DADS) on hippocampal synapses and learning and memory abilities in a mouse model of A1zheimer's disease (AD).
METHODSMouse models of AD established by agglutinated Aβ1-42 injection in the lateral cerebral ventricle were randomized into 4 groups and treated with DADS at the daily doses of 0, 10, 50 and 100 mg/kg by gavage for 30 consecutive days. The learning and memory abilities of the mice were assessed with Morris water maze test; the structures of the dendritic spines and synapses in CA1 region of the hippocampus were observed under transmission electron microscope with silver staining; PSD95 and SYP protein and mRNA expressions in the hippocampus were detected with Western blotting and RT-PCR.
RESULTSCompared with the AD model mice, the mice treated with 50 and 100 mg/kg DADS showed enhanced learning and memory abilities in Morris water maze test. The dendritic spines and synapses in CA1 region of the hippocampus increased obviously and hippocampal expressions of PSD95 and SYP were enhanced in mice treated with 50 and 100 mg/kg DADS.
CONCLUSIONDADS at the daily doses of 50 and 100 mg/kg can improve the learning and memory abilities and increase the number of dendritic spines and synapses in the hippocampus in mouse models of AD.
Allyl Compounds ; pharmacology ; Alzheimer Disease ; drug therapy ; Animals ; CA1 Region, Hippocampal ; drug effects ; Disease Models, Animal ; Disulfides ; pharmacology ; Learning ; Male ; Memory ; Mice ; Synapses ; drug effects
7.Fmoc solid-phase synthesis of cyclopeptide FIK.
Junjun ZHANG ; Wei SHI ; Wuling CHEN
Chinese Journal of Biotechnology 2008;24(3):468-472
We study the techniques of synthesis of disulfide bond-bearing cyclopeptides FIK. This experimentation with the material of Fmoc-aa use Solid-Phase synthesis after condensation by HBTU/HOBt/DIEA to synthesize linear peptide, then cyclopeptide was synthesized by creation of intramolecular disulfide bond by means of 12 oxidation of bis cysteine sulfhydryl of the linear peptide. The crude production was cleaved from the resin together with all protecting group and identified and separated by MALDI-MS and RP-HPLC. The peptide yield was 18%, after purification the purity was more than 97%. It was identified on MALDI-MS and Ellman reagent detection. This method is effective, simple, rapid and obtained good yield, and it's fit for the large-scale production.
Amino Acids
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chemistry
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Combinatorial Chemistry Techniques
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methods
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Disulfides
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chemistry
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Fluorenes
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chemistry
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Molecular Structure
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Peptides, Cyclic
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chemical synthesis
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chemistry
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
8.Trans-splicing of Cys mutated coagulation factor VIII.
Fu-Xiang ZHU ; Ze-Long LIU ; Jing MIAO ; Hui-Ge QU ; Xiao-Yan CHI
Acta Pharmaceutica Sinica 2012;47(6):734-738
To investigate the improving effect of inter-chain disulfide formation on protein trans-splicing, we introduce a Cys point mutation at Tyr(664) in heavy chain and at Thr(1826) in light chain of B-domain-deleted FVIII (BDD-FVIII). By co-transfection of COS-7 cell with the two Cys mutated chain genes, the intracellular protein splicing, inter-chain disulfide formation, secreted BDD-FVIII and bioactivity in culture supernatant were observed. The data showed that a strengthened spliced BDD-FVIII with an inter-chain disulfide detected by Western blotting and an elevated secretion of spliced BDD-FVIII (128 +/- 24 ng mL(-1)) compared to control (89 +/- 15 ng mL(-1)), assayed by a sandwich ELISA. A Coatest was performed to assay the secretion of bioactivity in culture supernatant and shown a much higher value (0.94 +/- 0.08 u mL(-1)) compared to that of control (0.62 +/- 0.15 u mL(-1)). It suggests that inter-chain disulfide formation could improve protein trans-splicing based dual-vector delivery of BDD-FVIII gene providing experimental evidence for ongoing in vivo study.
Animals
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COS Cells
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Cercopithecus aethiops
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Cysteine
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genetics
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metabolism
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Disulfides
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metabolism
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Factor VIII
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genetics
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metabolism
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Gene Transfer Techniques
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Genetic Vectors
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Mutation
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Peptide Fragments
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genetics
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metabolism
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Protein Splicing
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Transfection
9.Effects of preoperatively selected gut decontamination on cardiopulmonary bypass-induced endotoxemia.
Jiang YU ; Ying-Bin XIAO ; Xian-Yuan WANG
Chinese Journal of Traumatology 2007;10(3):131-137
OBJECTIVETo evaluate the influence of preoperatively selected gut decontamination (SGD) on intestinally derived endotoxemia(ETM) in patients with rheumatic heart disease undergoing valve replacement operation with cardiopulmonary bypass(CPB).
METHODSThirty patients were randomly divided into control group and SGD group. The patients in control group underwent preoperative bowel preparation, i.e, diet preparation and enema. The patients in SGD group were administrated 100 mg Tobramycin, 40 mg garlicin and 20% Lactulose for 10 ml three times per day for 3 days besides routinely preoperative bowel preparation. Bacteria cultivation and identification and Gram staining of feces in both groups were used to evaluate species of intestinal flora and their ratios. The levels of endotoxin, D-lactate, TNF-alpha and complement 3 were determined at four time points of anesthetic induction, CPB end, 2 h after CPB, 24 h after CPB. And the related clinical biochemical and clinical markers were recorded.
RESULTSAerobic gram-negative bacilli (AGNB) ratio in post-SGD group decreased significantly as compared with that in control group and pre-SGD group (P less than 0.05). The level of D-lactate reduced significantly at time points of anesthetic induction and 2 h after CPB (P less than 0.05). Endotoxin levels of patients in both groups elevated significantly after CPB (P less than 0.05), and endotoxin levels of the patients in SGD group decreased significantly at points of CPB end (P less than 0.01) and 24 h after CPB (P less than 0.05) compared with those in control group. The levels of TNF-alpha and complement 3 were similar in both groups as well as clinical and biochemical markers.
CONCLUSIONSCPB induces endotoxemia, while the regime of SGD is an effective way to prevent endotoxemia but may not affect activation of inflammatory media and clinical outcomes.
Adult ; Allyl Compounds ; therapeutic use ; Cardiopulmonary Bypass ; adverse effects ; Decontamination ; Disulfides ; therapeutic use ; Endotoxemia ; prevention & control ; Humans ; Intestines ; microbiology ; Preoperative Care ; Rheumatic Heart Disease ; surgery ; Tobramycin ; therapeutic use
10.Effect of diallyl disulfide on expression and secretion of VEGF in HL-60 leukemic cells.
Yi XIE ; Zi-Li FAN ; Chen-Jiao YAO ; San-Qin TAN ; Ya-Li ZHAO
Journal of Experimental Hematology 2006;14(2):212-216
The study was aimed to investigate the expression of VEGF mRNA and VEGF protein in HL-60 cells treated with diallyl disulfide (DADS), and to explore the antileukemic mechanism of DADS in respect of VEGF production. Semi-quantitative RT-PCR and ELISA were used to detect the expression of VEGF mRNA and secretion of VEGF protein in HL-60 cell lines treated by DADS respectively. The results showed that the expression of VEGF mRNA and secretion of VEGF protein were found in HL-60 cells. The expression of VEGF mRNA and secretion of VEGF protein in HL-60 cells could be down regulated by treatment with 0.625, 1.25, and 2.5 microg/mL DADS for 48 and 72 hours and the effects had a dose dependent relationship (r > 0.9, P < 0.01). The differences between DADS treated HL-60 cell groups and the control group were statistically significant (P < 0.01), there were also statistically significant differences among three DADS-treated HL-60 cell groups (P < 0.05). It is concluded that DADS effectively inhibits the proliferation of human leukemia cell line HL-60 cells; DADS exerts its antileukemic effects by reduction of the expression of VEGF mRNA and VEGF protein secretion.
Allyl Compounds
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pharmacology
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Antineoplastic Agents
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pharmacology
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Cell Proliferation
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drug effects
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Disulfides
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pharmacology
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HL-60 Cells
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Humans
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RNA, Messenger
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biosynthesis
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genetics
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Vascular Endothelial Growth Factor A
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biosynthesis
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genetics