1.Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear.
Sun Joo LEE ; Hae Jin JEONG ; Ji Eun LEE ; Jong Soo LEE ; Ying Hua XUAN ; Hyun Hee KONG ; Dong Il CHUNG ; Mee Sun OCK ; Hak Sun YU
The Korean Journal of Parasitology 2006;44(4):313-320
In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patient's ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.
*Sequence Analysis, DNA
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RNA, Ribosomal, 18S/genetics
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Polymorphism, Restriction Fragment Length
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Parasitic Sensitivity Tests
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Myopia/therapy
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Itraconazole/administration & dosage
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Humans
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Female
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Disinfectants/administration & dosage
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DNA, Ribosomal/analysis
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DNA, Protozoan/analysis
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DNA, Mitochondrial/analysis
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Contact Lenses/*adverse effects
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Chlorhexidine/administration & dosage
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Biguanides/administration & dosage
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Astigmatism/therapy
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Antiprotozoal Agents/administration & dosage
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Animals
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Adolescent
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Acanthamoeba Keratitis/drug therapy/*parasitology
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Acanthamoeba/classification/*genetics/*isolation & purification