BACKGROUND: Although several kinds of treatment methods(destructive therapy, immunotherapy, etc) for plane warts have been attempted but there have been no entirely satisfactory treatments, because the plane wants are seen to recur frequently. OBJECTIVE: This study was aimed to evaluate the combination therapy of griseofulvin and immunotherapy(dinitrochlorobenzene=DNCB, diphenylcycloprope none=DPCP) on plane warts. METHOD: Sixteen patients(age range, 9 to 41; mean age, 21.8 years) with VPJ were treated with the combination therapy with griseofulvin(500mg/day) and single contact immunotherapy(DNCB or DPCP).
Dinitrochlorobenzene ; Griseofulvin* ; Immunotherapy ; Warts*

Fifty-one cases of various warts were treated with 1-cbloro-2,4-dmitrobenzene (DNCB), and divided these warts into two groups by the method of treatment. The first group was treated by challenge on normal skin a,fter sensitization and the second group was treated by challenge on wart lesion after sensitization. The number of first group was 27 consisted of 13 of verruca vulgaris, 12 of verruca plana and 2 of condyloma acuminatum. The number of second group was 24 consisted of 10 of verruca vulgaris, 11 of verruca plana and R of verruca plantaris. The results are summarized as follows; 1. Thirty-one of all 51 cases (60. 7%) were cured. 2. Fifteen of 27 cases (55.5%) were cured in first group. 3. Sixteen of 24 cases (66. 6%) were cured in second group. 4. All 3 cases of verruca planaris were cured in second group. 5. Eight cases were cured only by sensitization and consisted of 6 of verruca plana and of verruca vulgaris. 6. There was no significant difference in cure rates of warts between the group within 2 years of duration and the group over 2 years of duration. 7. There were no severe adverse reactions.
Dinitrochlorobenzene* ; Immunotherapy* ; Skin ; Warts*

No abstract available.
Animals ; Dinitrochlorobenzene* ; Guinea Pigs* ; Guinea*

No abstract available.
Child* ; Dinitrochlorobenzene* ; Humans ; Immunotherapy* ; Warts*

Tolerance to contact hypersensitivity was induced by feeding of different DNCB doses in mice. A total of 40 mice were divided into 4 groups(control group, 6 mg feeding group, 10 mg feeding group, 14 mg feeding group) in experiment I, Degree of tolerance to contact hypersensitivity was rneasured by incremert rate of ear swelling after challenge with DNFB. Experiment 2 was performed in the same method of cxperiment: I with addition of 3 mg DNCB feeding group. The increment ratee were significantly decreased in DNCB feeding groups in experirnent 1 and 2(p<0.0l). But there were no differences statisticalIy between increment rates of DNCB feeding groups.
Animals ; Dermatitis, Contact ; Dinitrochlorobenzene* ; Dinitrofluorobenzene ; Ear ; Mice*

The current experiment pursued the time course of UVB-induced immune suppression of DNCB contact hypersensitivity(CHS) in guinea pigs. Both group B and C, received suberythemal doses of UVB on days -3, -2, -1, 0 and -6, -5, -4, 3 respectively, showed marked suppressions of CHS (56.36p;and56.25p,). However, the recovery of CHS from the UVB-induced suppression started by group D(UVB on days -9, -8, -7, -6: 67. 36%) and further extended to subsequent group E(UVB on days -12, -11, -10, -9: 78.82%), Group F(UVB on days -15, -14, -13, -12: 79.86%) and the final group G(UVB on days -18, -17, -16, -15: 81. 94%) in which the recovery of CHS slightly exceeded the control (group A:79. 86%).
Animals ; Dermatitis, Contact* ; Dinitrochlorobenzene* ; Guinea Pigs* ; Guinea*

Warts are benign turnors caused by oncogenic human papovavirus which has been clearly identified on electronmicroscopy, although repeated atternpts at culture have not been successful. Various therapeutic modalities have been tried for the treatment of warts, however thete is no saisfactory method and the clinical course as well as the prognosis is always variable. Recently several authors have reported successful therapeutic results in the treatment of warts by DNCB sensitization method based on the findings that warts are often regressing spontaneously and that the spontaneous regression seems to depend on host's ability to mount an immune response against the wert virus. The present study was undertaken to evaluate the DNCB sensitization method for possible routine use in the treatment of warts. Fifteen cases including five verruca vulgaris and ten verruca plana juvenilis patients were sensitized with 1000ug of DNCB in acetone solution on either side of their inner forearms. Seven or ten days after sensitization, several warts, which were unintentionally selected, were challenged with 0.05 to 0.1ml of weaker DNCB solution(50ug-25ug/0.1ml)at weekly interval. Four patients with verruca vulgaris and three patients with verruca plana juvenilis did not return after 1 to 4 times of challenge, however six out of these 7 patients showed a slight decrease in size and number of the warts. One patient with verruca plana juvenilis developed marked irritative and delayed cutaneous hypersensitivity reaction to the 3 times of challenge, so that this patient was excluded from further study. The remaining 7 patients consisted of I verruca vulgaris and 6 verruca plana juvenilis patients showed complete disappearance of all warts including those which were never challenged, after 1 to 13 times of DNCB challenge application. The authors reviewed the possible therapeutic mechanism of DNCB sensitization in the wart patients, the possible advantage and disadvantage of this method and concluded that this method can be cautiously used in seleted patients with warts whose response to the conventional treatment methods are poor.
Acetone ; Dinitrochlorobenzene* ; Forearm ; Humans ; Hypersensitivity ; Prognosis ; Warts*

The effects of histamine type 2(H2) receptor antagonists, cimetidine and ranitidine, on the induction of contact hypersensitivity and UVB induced suppression of contact hypersensitivity were studied. Our experiment showed that treatment with cimetidine or ranitidine slightly enhanced contact hypersensitivity to DNCB, but the suppression of contact hypersensitivity elicited by UVB were moderatly reversed by ranitidine. These facts suggest that H2-receptor antagonist may be responsible for th blocking histamine induced suppressor factor by acting upon UV-induced suppressor T cell.
Cimetidine ; Dermatitis, Contact* ; Dinitrochlorobenzene ; Histamine ; Ranitidine

Previously we reported the result of topical immunotherapy of warts with I -chloro-2,4-dinitrobenzene(DNCB) solution using two different methods. In this study, we treated the warts with DNCB ointment and compared the therapeutic result with previous study. In addition, in order to evaluate the possible rnechanlarn of DNCB-induced wart regression by histologic rnethod, 5 regressing warts were biopsied and we observed the histopathologic findings. Thirty-six patients with various warts which were resistant to the conventional measures were treated by challeng application with 100mg% or 10mg% DNCB ointment directly on several randomly selected wart lesions after sensitization. with l?000mcg of DNCR in solution on normal skin of upper arm. In 5 pat:ients showing regression of warts, one wart lesion which was not challenged with DNCR was biopsied. The results are summarized as follows; 1. We treated the 36 patients having various warts with DNCB ointment and 25 patients (69.4%) were cured. Most(72%) of the eured warts began to regress within one month. 2. We cornpared the therapeutic result in this study with the results in theprevious study in which we used DNCB solution with two different methods, Comparing the results obtained by the R different methods, we found no statistically different cure rate, however the present method might be more convement because the ointment can be maintained longer without any change of the ingredient concentration and can be applied more easily than the solution. 3. On histologic examination, we observed moderate to severe patchy perivasce
Arm ; Dinitrochlorobenzene* ; Humans ; Immunotherapy* ; Skin ; Warts*

The aims of study presented here were to determine dose-dependent effects of UVB irradiation on CHS reaction and comparative effects of VVB on the induction and elicitation in guinea pigs. Pre-induction exposure to either suberythemal or supraerythemal doses of UVB resulted in suppreasion of CHS (53.47% & 57.64%) campared to positive control (79. 86%) However, suberythemal doses of UVB generated a more potent suppression than supraerythemal doses. Pre-elicitation exposure to suberythemal doses of UVB on the elicitation sites and adjacent to elicitation sites resulted in suppressed CHS reactions in both groups (60.4zg & 56.25p,). When the reaction by UVB was eliminated from the cutaneous reaction elicited in guinea pigs sensitized to DNCB, by UVB irradiation on the adjacent to elicitation sites, generated a more potent suppression than that UVB was directly irradiated on the elicitation sites.
Animals ; Dermatitis, Contact* ; Dinitrochlorobenzene* ; Guinea Pigs* ; Guinea*