1.Establishment of scientific research performance evaluation index system based on Delphi method and Multi-AHP for medical universities in western China developing areas
Dingyun YOU ; Ting HE ; Lihua LI
Chinese Journal of Medical Science Research Management 2017;30(4):263-267
Objective Taking into account of the actual scientific research conducted at medical universities in Yunnan Province,Southwest China,to develop an appropriate evaluation index system of scientific research performance.Methods main methodologies and procedures adopted to develop the evaluation index system include literature analysis,Delphi method,Group Analytic Hierarchy Process and Expert authority calculation.Results The evaluation index system of scientific research performance consists of scientific research input index system and output index system.The scientific research input index system includes financial input and human resources input as two primary indicators.While the scientific research output index system is made up of two primary indicators,named quantitative output and qualitative output.There are another six second-level indicators,six third-level indicators and 37 fourth-level indicators to make up of the quantitative output index system.Calculation of the indicator weight will consider the natures of different units or departments,the mixed analysis of quantitative and qualitative methods,as well as one-vote veto decision were adopted to make sure classified assessment were actually implemented during the evaluation process.Conclusions The index system is suggested to be used for evaluating scientific research performance in different colleges or departments.Adopting such system can help the management of scientific research performances at both micro-and meso-levels.It may also inspire more researchers and research management personnel in a broader sense.
2.Effects of CYP3A5 * 3 polymorphisms on tacrolimus pharmacokinetic in renal transplantation recipients by PyrosequencingTM
Dingyun LI ; Zhi LI ; Lijun ZHU ; Qifa YE ; Bing DU ; Guo WANG ; Yingzi MING ; Ke CHENG ; Xingguo SE ; Honghao ZHOU
Chinese Journal of Organ Transplantation 2010;31(5):280-283
Objective To study the influence of CYP3A5 genetic polymorphism on tacrolimus metabolism in renal transplantation recipients and evaluate the methods of detecting CYP3A5* 3 polymorphism. Methods Ninety-seven renal recipients receiving the triple immunosuppressive (tacrolimus + mycophenolate mofetil + prednison) were genotyped for CYP3A5* 3 polymorphisms by the Pyrosequencing TM assays. Tacrolimus trough concentration of the patients was measured by enzyme multiplied immunoassay technique, and concentration/adjusted dose ratio (C/D) was investigated at the 7th day, 14th day, 1st month, 3rd month and 6th month after renal transplantation. Results The Pyrosequencing TM assays allowed for quick and accurate detection of CYP3A5* 3 genotypes. There were CYP3A5* 1/* 1 genotype in 17 cases (17. 5%), * 1/* 3 in 35cases (36. 1 %) and *3/* 3 in 45 cases (46.4 %) identified in 97 renal recipients. The C/D of CYP3A5 * 1/* 1 and * 1/* 3 patients was significantly lower than that of * 3/* 3 patients (P<0. 01)after renal transplantation. Conclusion The CYP3A5* 3 polymorphisms are significantly correlated with tacrolimus pharmacokinetic in renal transplant recipients. Detecting the CYP3AS* 3 genotype of the recipient before the transplantation by the Pyrosequencing TM assays can be used to help determine the optimal initial dosage after transplantation, resulting in individualized drug therapy.
3.Application of quantum dots immune fluorescent labelling in colorectal cancer tissues
Fengping HE ; Xin XU ; Qingsong WU ; Dingyun LI ; Zhanzhong MA ; Sheng TANG ; Weidong YIN ; Haitao GONG ; Hengxian LIN
International Journal of Laboratory Medicine 2016;37(18):2531-2533
Objective To investigate different antigens detected by a novel labelled reagent‐quantum dots(QDs) in the colorectal cancer tissues microarray(TMA) .Methods Depend on QDs streptavidin conjugate(QDs‐SA) combined specially with biotinylation IgG ,immune of luorescent histochemistry was utilized to examine expression of K‐ras ,matrix‐remodeling associated 5(MXRA5) proteins in the colorectal cancer TMA ,where the protein accurate location was observed .Results K‐ras ,matrix‐remodeling associ‐ated 5(MXRA5) proteins were high expressed in colorectal cancer tissue and located accurately in the cell membrane and nucleus of colorectal cancer cells ,respectively .Conclusion QDs exhibit excellent photostability ,broad emission spectrum and long fluorescence lifetime .Modified with streptavidin could accurately detect different protein locations in the colorectal cancer TMA .This is a novel approach for studying targeted imaging of colorectal cancer in vivo and vitro clinical diagnosis .
4.Clinical application of real-time fluorescence quantitative PCR for the detection of fecal tumor M2-pyruvate kinase in colorectal cancer patients
Yulan LIU ; Fengping HE ; Xin XU ; Qingsong WU ; Dingyun LI ; Zhanzhong MA ; Yanle GUO ; Sheng TANG ; Weidong YIN ; Haitao GONG ; Yi LIU ; Hengxian LIN
International Journal of Laboratory Medicine 2017;38(11):1444-1446
Objective To investigate the application valve of real-time fluorescence quantitative polymerase chain reaction(RT-PCR) for the detection of tumor M2-pyruvate kinase(tM2-PK) DNA in patients with colorectal cancer(CRC).Methods Fragment of tM2-PK DNA(162 bp) was amplified and inserted into PGM-T vector to construct recombinant plasmid,which was used to develop RT-PCR method.Sensitivity,specificity and repeatability of RT-PCR for the detection of tM2-PK were analyzed.From Jan.2014 to Jun.2016,200 CRC patients and 100 healthy subjects were enrolled and detected for fecal and serum tM2-PK DNA by using RT-PCR,and the detected results were compared with those detected by using enzyme linked immunosorbent assay(ELISA).Results Recombinant plasmid was successfully constructed,which was certified by sequencing.The sensitivity of RT-PCR for the detection of tM2-PK DNA was 10 copy/mL,with high specificity and 0.3%-2.9% of coefficient of variation.In patients,the positive rate of fecal tM2-PK DNA,detected by RT-PCR,was 92.50%,and that of ELISA to detect tM2-PK was 80.00%.Fecal and serum levels of tM2-PK were correlated with the pathologic stages of tumour.Conclusion Self-established RT-PCR could be specificity and sensitivity for the detection of fecal tM2-PK,which could be used for the early diagnosis of CRC.
5.Difference of the clinical characteristics and outcome between interstitial lung disease with dermatomyositis and idiopathic pneumonia fibrosis
Dingyun FENG ; Xiaoling ZOU ; Hailing YANG ; Heliang LI ; Yuqi ZHOU ; Tiantuo ZHANG
Journal of Chinese Physician 2018;20(10):1498-1501
Objective To compare the clinical features and prognosis between dermatomyositis-associated interstitial lung disease (DM-ILD) and idiopathic pulmonary fibrosis (IPF).Methods Patients with interstitial lung disease with dermatomyositis (DM-ILD) or idiopathic pneumonia fibrosis (IPF) from January 2003 to March 2014 in the third affiliated hospital of Sun Yat-sen University were included.Results Among the 64 patients enrolled,44 were DM-ILD and 20 were IPF.IPF was more common in the elderly (P =0.000),men (P =0.004) and smokers (P =0.000),and its high-resolution computed tomography (CT) mostly showed grid shadow (P=0.014) and honeycomb shadow (P=0.000).DM-ILD usually had cough symptoms (P =0.025).High-resolution CT showed patchy (P =0.048) and banded (P =0.000).Glucocorticoid (P =0.000) and immunosuppressive agents (P =0.000) were commonly used in the treatment of DM-ILD.However,there was no significant difference in 90d mortality between the two groups (P > 0.05).Conclusions IPF is more common in the elderly,men and smokers,and its high-resolution CT mostly shows grid shadow and honeycomb shadow,distribution is diffuse.DM-ILD often has cough symptoms,and its high resolution CT is mostly plaques and streaky shadows.Glucocorticoids and immunesuppressants are commonly used in DM-ILD,but there is no significant difference in 90-day mortality between them.
6.Effect of genetic polymorphism on disposition of calcineurin inhibitors in solid organ transplantation.
Dingyun LI ; Lijun ZHU ; Qifa YE
Journal of Central South University(Medical Sciences) 2010;35(9):1018-1022
Calcineurin inhibitors, tacrolimus and cyclosporine, characterized by a narrow therapeutic index and a high variability in pharmacokinetic behaviors, are 2 basic immunosuppressive drugs widely used in solid organ transplantation. Tailoring of immunosuppressive drug therapy to the specific requirements of individual patients to optimize the efficacy and minimize the toxicity remains one of the biggest challenges for doctors in solid organ transplantation. Pharmacogenetic and pharmacogenomic researches, studying the effect of genetic polymorphism encoding drug metabolizing enzymes, drug transporters and pharmacological target molecules on drug disposition and action, hold promise to produce useful clinical tools for individualizing immunosuppressive therapy.
Animals
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Calcineurin Inhibitors
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Cyclosporine
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pharmacokinetics
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pharmacology
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Cytochrome P-450 CYP3A
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genetics
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Humans
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Immunosuppressive Agents
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pharmacokinetics
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pharmacology
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Organ Transplantation
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Polymorphism, Genetic
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Tacrolimus
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pharmacokinetics
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pharmacology
7.Entropy、Dissipative Structure And Health
Sifan DUAN ; Mengmei LIU ; Yani LI ; Dingyun YOU
Journal of Kunming Medical University 2024;45(3):1-6
Dissipative structure refers to a self-organized and orderly structure that exists far from equilibrium.The human body,considered a classical example,generates negative entropy through the exchange of matter,energy,and information with the environment to counteract the increase in entropy.In this paper,we organized theories and related research on dissipative structure and entropy,discussing their significance in regulating various aspects such as the human body,cancer,aging,and more.By selecting the special population of pregnant women,focusing on the information dimension,developing the corresponding information exchange scale(Cronbach's α>0.9),and proposing the information exchange index,we preliminarily explored the influence of the dissipative structure's information dimension on pregnancy health.The results showed a negative correlation between the information exchange index and anxiety scores during pregnancy(r =-0.35,P<0.001),with an OR value of 0.26(95%CI:0.08~0.80),preliminarily confirming the feasibility of conducting empirical research based on dissipative structure theory.If further relevant empirical studies are conducted,it is expected that new disease prevention strategies will be developed and new theories and methods will be provided for the field of public health.
8. Analysis of teaching quality and influencing factors of undergraduate teachers in a Medical University in western China
Shunyue YANG ; Shan YAN ; Jianyun YU ; Chuanzhi XU ; Guofeng SONG ; Faqian LU ; Fuli LI ; Weitang SHAO ; Dingyun YOU
Chinese Journal of Medical Education Research 2019;18(12):1244-1248
Objective:
In order to understand the current situation and influencing factors of teaching quality in a Medical University in Yunnan, thus improving the teaching quality of the teachers.
Methods:
The self-made evaluation forms for teachers' teaching quality which include 9 first-level indicators were adopted. In December 2016, a survey was conducted on some students from grade 2013 to 2016 about the teachers who gave them lectures from September to December 2016, involving 7 different majors, 23 teachers and 18 courses. SPSS 21.0 was used for data analysis. Enumeration data were described by frequencies and percentages. Univariate and multivariate